CN1648232A - Separating technology for oil removing strain for refining silk spinning raw material - Google Patents
Separating technology for oil removing strain for refining silk spinning raw material Download PDFInfo
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- CN1648232A CN1648232A CN 200410093041 CN200410093041A CN1648232A CN 1648232 A CN1648232 A CN 1648232A CN 200410093041 CN200410093041 CN 200410093041 CN 200410093041 A CN200410093041 A CN 200410093041A CN 1648232 A CN1648232 A CN 1648232A
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- oil removing
- raw material
- removing strain
- silk spinning
- spinning raw
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Abstract
The present invention relates to separation technology for oil removing strain for refining silk spinning raw material and belongs to the field of textile material processing technology. By means of double layer agar plate inoculation, culture medium A and sample liquid are inoculated onto plate to obtain colony for generating transparent circle; and the colony is inoculated on beef ointment peptone slant and maintained in refrigerator. The seed is inoculated in culture medium B inside Erlenmeyer flask, and cultured on shaking table to obtain the oil removing strain. The present invention has unique separation technology to screen out optimal strain for removing strain for refining silk spinning raw material.
Description
Technical field
The present invention relates to a kind of isolation technique of oil removing strain for refining silk spinning raw material, belong to the preliminary working technical field of textile raw material.
Background technology
Silk spinning raw material concise is in order to remove most of silk gum in the raw material, grease, impurity etc., to give full play to silky fibre inherent gloss and feel, keep the original physical and mechanical properties of silky fibre simultaneously as far as possible.Concise quality has influence on directly that can the back operation normally carry out, the quality of quality product and the height of production cost, and the Different Effects of method for refining reaches the pollution to environment to working strength of workers, work situation.
At present, on the silk spinning industry production there be method for refining commonly used: method and biological enzyme are practiced in soap alkaline process, corruption.The soap alkaline process is traditional method for refining, system adopts chemical agent to promote to come unstuck oil removing, speed is fast, output is high, but it is heavy to fibre-tendering, it is difficult that the grease of heavy oil feedstock is removed, often cause that residual gum content is on the low side, the raising of production cost, and the chemical agent environmental pollution is bigger in order to strengthen oil removing.Biological enzyme directly acts on silk spinning raw material with biological enzyme formulation exactly, can raise labour productivity, improve work situation, reduce environmental pollution and fibre-effects is relaxed, but prior biological zymin cost height, optional kind are few, are unsuitable for the application that factory mass is produced.
It is to utilize the action of microorganisms that exists in the physical environment to the silk spinning raw material oil removing of coming unstuck that method is practiced in corruption, is a spontaneous fermentation process.The corruption system is made up of multiple microorganism, and some is useful bacterial classification, and some is useless bacterial classification or even harmful bacterial classification.When the control aborning of this method is proper, have unique functional oil that removes, production cost is low, and the experienced liquid and the ecotope consistency of discharging are good, biodegradable.But the production cycle is long, and labour productivity is low, produces foul smell in the production process, and sanitary condition is poor, and the raw material residual gum content is on the low side, unstable product quality.
Summary of the invention
The isolation technique that the purpose of this invention is to provide a kind of bacterial strain of suitable refining silk spinning raw material oil removing, the bacterial strain that obtains are used for the concise concise quality that improves silk spinning raw material, reduce the concise time, improve work situation, reduce environmental pollution.
The present invention is the isolation technique of oil removing strain for refining silk spinning raw material, it is characterized in that carrying out as follows:
(1) preparation of sample to be separated: gather the silk spinning raw material corruption and practice liquid or silk spinning factory soil sample, the silkworm chrysalis that rots etc. is used for separating oil removing strain;
(2) preliminary screening of oil removing strain: adopt double-deck agar plate method inoculation, culture medium A and sample liquid are coated with inoculation on flat board, obtain producing the bacterium colony of transparent circle, colony inoculation on the beef extract-peptone inclined-plane, is preserved in refrigerator;
(3) the multiple sieve of oil removing strain: bacterial classification inoculation in the triangular flask that substratum B is housed, is placed on the shaking table and cultivates, and obtaining acting on silk spinning raw material and making the raw material residual oil content is minimum bacterial classification, is oil removing strain;
(4) preservation of oil removing strain: isolated oil removing strain is inoculated on the beef-protein medium inclined-plane places refrigerator to preserve.
The prescription of described culture medium A can be: the upper strata: water 1000ml, extractum carnis 1.0～10.0g, peptone 1～10g, NaCl 1.0～6.0g, agar 15.0～20.0g, pH6.5～9.0; Lower floor: water 1000ml, sweet oil 1～10ml, agar 15.0～20.0g, pH6.5～9.0.
The prescription of described substratum B can be: water 1000ml; Silk spinning raw material 80g; (NH
4)
2SO
41.0～10.0g; K
2HPO
41.0～10.0g; NaCl 1.0～6.0g; PH7.0～9.0.
The prescription of described beef-protein medium can be: water 1000ml, extractum carnis 1.0～5.0g, peptone 1～10g, NaCl 1.0～6.0g, agar 15.0～20.0g, pH7.0～9.0.
The inoculum size of described preliminary screening operation middle plateform coating inoculation can be 0.05～0.2ml, and inoculation temp is 30～45 ℃, and incubation time is 1～4 day.
The speed of shaking of shaking table can be 100～300r/min in described multiple riddler's preface, and culture temperature is 30～45 ℃, and incubation time is 24～96h.
The storage temperature of described oil removing strain in refrigerator can be 0～10 ℃.
Described culture medium A, substratum B shakes bottle, the 20～30min that can in Autoclave, sterilize respectively such as inclined-plane, the vapor pressure of Autoclave is 0.7～1.2kg/cm
2
The present invention's microbe groups in to existing corruption white silk liquid is analyzed, and by multiple separation method being compared on the basis of experiment, form unique isolation technique, filtered out the dominant strain of suitable refining silk spinning raw material oil removing, compared with prior art, have screening effect obviously, easily observe, outstanding advantage and positively effect such as easy and simple to handle.The advantage oil removing strain that filters out can directly act on the oil removing of silk spinning raw material, solved prior art production cycle long, work situation is poor, the shortcoming of unstable product quality, can effectively improve concise quality, reduce the concise time, raise labour productivity, improve work situation, reduce environmental pollution.
Embodiment
Embodiment 1:
(1) preparation of sample to be separated: gather the liquid of silk spinning raw material corruption after 24 hours, use for separating oil removing strain.
(2) preliminary screening of oil removing strain: the double-deck agar plate method of the screening method of oil removing strain.With vessel and culture medium A respectively at 1kg/cm
2Sterilization 20min.The sterilization back is fallen dull and stereotyped, adopts the coating method inoculation, and inoculum size is 0.1ml.37 ℃, cultivated 1～3 day.Obtain the bacterium colony of the oil-removing bacteria of three kinds of forms.
The prescription of culture medium A is: the upper strata: water 1000ml, extractum carnis 3.0g, peptone 5g, NaCl 5.0g, agar 17.0g, pH7.5; Lower floor: water 1000ml, sweet oil 5ml, agar 17.0g, pH7.5.
(3) the multiple sieve of oil removing strain: with vessel and substratum B respectively at 1kg/cm
2Sterilization 20min, inoculation then.To inoculate the good bottle that shakes again and place and cultivate on the shaking table, 39 ℃ of controlled temperature shake speed 220r/min, take out after cultivating 64h, measure the raw material residual oil content, obtain the minimum bacterial classification of residual oil content.
The prescription of substratum B is: water 1000ml; Silk spinning raw material 80g; (NH
4)
2SO
45g; K
2HPO
45.0g; NaCl2.0g; PH8.0;
(4) by the aforesaid method oil removing strain of gaining the upper hand.
Embodiment 2:
(1) preparation of sample to be separated: gather the silk spinning soil sample 1g of factory, add the 99ml sterilized water, dilution vibration back is used for separating oil removing strain.
(2) preliminary screening of oil removing strain: the double-deck agar plate method of the screening method of oil removing strain.
The prescription of culture medium A is: the upper strata: water 1000ml; Extractum carnis 4.0g; Peptone 4g; NaCl 6.0g; Agar 17.0g; PH8.5.Lower floor: water 1000ml; Sweet oil 7ml; Agar 17.0g; PH9.0.With vessel and substratum respectively at 1kg/cm
2Sterilization 20min.The sterilization back is fallen dull and stereotyped, adopts the coating method inoculation, and inoculum size is 0.2ml.40 ℃, cultivated 3～5 days.Obtain the bacterium colony of the oil-removing bacteria of three kinds of forms.
(3) the multiple sieve of oil removing strain:
The prescription of substratum B is: water 1000ml; Silk spinning raw material 80g; (NH
4)
2SO
43g; K
2HPO
45.0g; NaCl 2.0g; PH9.0; With vessel and substratum respectively at 1kg/cm
2Sterilization 20min.Inoculation then.To inoculate the good bottle that shakes again places and cultivates 36 ℃ on the shaking table; 200r/min; Take out after cultivating 72h, measure the raw material residual oil content, obtain the minimum bacterial classification of residual oil content.
(4) by the aforesaid method oil removing strain of gaining the upper hand.
Embodiment 3:
(1) preparation of sample to be separated: gather septic silkworm chrysalis 1g, add the 99ml sterilized water, dilution vibration back is used for separation advantage oil removing strain.
(2) preliminary screening of oil removing strain: the double-deck agar plate method of the screening method of oil removing strain.
The prescription of culture medium A is: the upper strata: water 1000ml; Extractum carnis 5.0g; Peptone 8g; NaCl 5.0g; Agar 17.0g; PH9.0.Lower floor: water 1000ml; Sweet oil 2ml; Agar 17.0g; PH7.0.With vessel and substratum respectively at 1kg/cm
2Sterilization 20min.The sterilization back is fallen dull and stereotyped, adopts the coating method inoculation, and inoculum size is 0.1ml.45 ℃, cultivated 3～5 days.Obtain the bacterium colony of the oil-removing bacteria of three kinds of forms.
(3) the multiple sieve of oil removing strain:
The prescription of substratum B is: water 1000ml; Silk spinning raw material 80g; (NH
4)
2SO
48.0g; K
2HPO
45.0g; NaCl 2.0g; PH7.0; With vessel and substratum respectively at 1kg/cm
2Sterilization 20min.Inoculation then.To inoculate the good bottle that shakes again and place and cultivate on the shaking table, 100r/min, 45 ℃ are taken out after cultivating 48h, measure residual oil content, obtain the minimum bacterial classification of residual oil content.
(4) by the aforesaid method oil removing strain of gaining the upper hand.
Claims (8)
1, a kind of isolation technique of oil removing strain for refining silk spinning raw material is characterized in that carrying out as follows:
(1) preparation of sample to be separated: gather the silk spinning raw material corruption and practice liquid or silk spinning factory soil sample, the silkworm chrysalis that rots is used for separating oil removing strain;
(2) preliminary screening of oil removing strain: adopt double-deck agar plate method inoculation, culture medium A and sample liquid are coated with inoculation on flat board, obtain producing the bacterium colony of transparent circle, colony inoculation on the beef extract-peptone inclined-plane, is preserved in refrigerator;
(3) the multiple sieve of oil removing strain: bacterial classification inoculation in the triangular flask that substratum B is housed, is placed on the shaking table and cultivates, and obtaining acting on silk spinning raw material and making the raw material residual oil content is minimum bacterial classification, is oil removing strain;
(4) preservation of oil removing strain: isolated oil removing strain is inoculated on the beef-protein medium inclined-plane places refrigerator to preserve.
2, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that the prescription of described culture medium A is: the upper strata: water 1000ml, extractum carnis 1.0～10.0g, peptone 1～10g, NaCl 1.0～6.0g, agar 15.0～20.0g, pH6.5～9.0; Lower floor: water 1000ml, sweet oil 1～10ml, agar 15.0～20.0g, pH6.5～9.0.
3, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that the prescription of described substratum B is: water 1000ml; Silk spinning raw material 80g; (NH
4)
2SO
41.0～10.0g; K
2HPO
41.0～10.0g; NaCl 1.0～6.0g; PH 7.0～9.0.
4, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that the prescription of described beef-protein medium is: water 1000ml, extractum carnis 1.0～5.0g, peptone 1～10g, NaCl 1.0～6.0g, agar 15.0～20.0g, pH 7.0～9.0.
5, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that the inoculum size of described preliminary screening operation middle plateform coating inoculation is 0.05～0.2ml, inoculation temp is 30～45 ℃, incubation time is 1～4 day.
6, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that the speed of shaking of shaking table in described multiple riddler's preface is 100～300r/min, culture temperature is 30～45 ℃, incubation time is 24～96h.
7, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that the storage temperature of described oil removing strain in refrigerator is 0～10 ℃.
8, by the isolation technique of the described oil removing strain for refining silk spinning raw material of claim 1, it is characterized in that described culture medium A, substratum B shakes bottle, the inclined-plane 20～30min that in Autoclave, sterilizes respectively, and the vapor pressure of Autoclave is 0.7～1.2kg/cm
2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410093041 CN1648232A (en) | 2004-12-10 | 2004-12-10 | Separating technology for oil removing strain for refining silk spinning raw material |
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|---|---|---|---|
| CN 200410093041 CN1648232A (en) | 2004-12-10 | 2004-12-10 | Separating technology for oil removing strain for refining silk spinning raw material |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899906A (en) * | 2012-10-16 | 2013-01-30 | 华东师范大学 | Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation |
| CN110106110A (en) * | 2019-04-26 | 2019-08-09 | 四川省丝绸科学研究院 | A kind of microbial strains and its application in silk spinning raw material oil removing |
-
2004
- 2004-12-10 CN CN 200410093041 patent/CN1648232A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102899906A (en) * | 2012-10-16 | 2013-01-30 | 华东师范大学 | Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation |
| CN102899906B (en) * | 2012-10-16 | 2014-10-29 | 华东师范大学 | Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation |
| CN110106110A (en) * | 2019-04-26 | 2019-08-09 | 四川省丝绸科学研究院 | A kind of microbial strains and its application in silk spinning raw material oil removing |
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