CN110106110A - A kind of microbial strains and its application in silk spinning raw material oil removing - Google Patents
A kind of microbial strains and its application in silk spinning raw material oil removing Download PDFInfo
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Abstract
The present invention discloses a kind of microbial strains and its application in silk spinning raw material oil removing, and the microbial strains are clostridium perfringen, is named as clostridium perfringenEnterobacterSp.TY-4, the microorganism are preserved in China typical culture collection center on June 3rd, 2018, and deposit number is CCTCC NO:M2018291；This microbial strains is to screen to obtain from silk spinning raw material corruption liquid, the microbial strains are directly acted on silk spinning raw material (or silk) by the present invention, the grease in silk spinning raw material can be effectively removed, and it can be further reduced the use of silk spinning raw material degreasing process chemical industry material, improve work situation, reduce pollution.
Description
Technical field
The present invention relates to microbial bacteria and its applied technical fields, specifically, a kind of microbial strains and its in silk spinning
Application in raw material oil removing.
Background technique
All kinds of spoiled cocoons, recotti, waste silk, the filature foot slag etc. that silk spinning raw material is rejected or generated from silk engineering.These
Leftover bits and pieces raw material is except being also stained with other pollutants and impurity etc. containing silk gum, in addition to grease, it is necessary to by concise arrangements removal grease,
Most of silk gum, wax etc..
The oil removing of silk spinning raw material is the main bugbear of thin,tough silk silk floss preparation, and currently used deoiling method has chemistry and biology-change
Two kinds of method.Chemical method mainly removes grease using chemical industry material high temperature scourings such as a large amount of bases, and effect is ideal not to the utmost, and dirty
Dye is serious；Immature earthen sites are first degenerated through spontaneous fermentation, using multiple-microorganism and the effect of mixed fungus fermentation system deposited,
Most grease is removed, then achievees the purpose that oil removing through more mild concise arrangement.But the system vulnerable to environmental factor such as
The influence of air, temperature, raw material sources etc. makes microbe species therein and quantity change, to influence the product of product
Matter and quality.
Therefore, the excellent de-oiling microorganism of screenability and it is applied in silk spinning raw material degreasing process, for into one
It walks improve refining silk spinning raw material quality, reduce environmental pollution etc. and have important practical significance.
Summary of the invention
The object of the present invention is to provide a kind of microbial strains and its applications in silk spinning raw material oil removing, available providing
While carrying out the microbial strains of silk spinning raw material de-oiling, provides and carry out silk spinning raw material oil removing using the microbial strains
Method.
The present invention is achieved through the following technical solutions: a kind of microbial strains can be used for silk spinning raw material oil removing, described micro-
Biological bacterial strain is clostridium perfringen, is named as clostridium perfringen Enterobacter sp.TY-4, the microbial strains are in 2018
It is preserved in China typical culture collection center on June 3, in, deposit number is CCTCC NO:M2018291.
A kind of application of microbial strains in silk spinning raw material oil removing, the microbial strains for silk spinning raw material (or
Silk) oil removing, remove the grease in silk spinning raw material (or silk).
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the microbial strains be used for silk spinning raw material oil removing when the following steps are included:
(1) preparation of oil removing microorganism；
(2) silk spinning raw material microorganism deoiling treatment；
(3) it washes: after de-oiling, taking out silk spinning raw material, wash with water；
(4) it dries: the silk spinning raw material after washing is dried；
(5) measurement of residual oil content.
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the step (1) the following steps are included:
(1.1) prepare seed culture medium: the beef extract of 3g/L, the peptone of 10g/L and the NaCl of 5g/L, pH are
7.0；
(1.2) microbial strains and glycerol are placed in -80 DEG C of guarantors at microorganism glycerol tube storing liquid
Hiding；
(1.3) described in (1.1) with inoculum concentration inoculation (1.2) described microorganism glycerol tube storing liquid of 1-2% (V/V)
Seed culture medium in, be placed in 37 DEG C of carry out shaking tables, 180rpm shake culture 12h obtains de-oiling microorganism.
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the proportion of the microbial strains and glycerol is 7:3.
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the step (2) the following steps are included:
(2.1) preparation of microorganism de-oiling culture medium: 10g/L peptone；2.5g/L sucrose；5g/L NaCl, 1g/L
SDS；pH 7.0；
(2.2) the de-oiling microorganism being prepared with the inoculum concentration inoculation step (1.3) of 1-2% (V/V) is in step
(2.1) in the microorganism de-oiling culture medium described in, silk spinning raw material is added by bath raio 2:100, in 37 DEG C of shaking tables, 180rpm concussion training
Support 48h.
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the washing concrete mode of the step (3) are as follows: clean using the water that water temperature is 30-40 DEG C de-
Silk spinning raw material after oil 2-4 times.
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the drying concrete mode of the step (4) are as follows: by the silk spinning raw material after washing in 100-110
2-4h is dried under the conditions of DEG C.
Further is that a kind of application of the microbial strains of the present invention in silk spinning raw material oil removing is better achieved,
Especially use following set-up modes: the measurement of the residual oil content in the step (5) is carried out according to soxhlet extraction methods.
Compared with prior art, the present invention have the following advantages that and the utility model has the advantages that
Clostridium perfringen Enterobacter sp.TY-4 provided by the invention can effectively degrade silk spinning within a short period of time
Grease in raw material.The method of grease removal is carried out compared with the conventional method using the microbial strains, operating method is easy,
Production process environmental protection, mild to fibration, fiber good luster that treated, strength are high, best in quality.
Detailed description of the invention
Fig. 1 is bacterial strain phylogenetic tree.
Specific embodiment
Embodiment is given below so that the present invention to be specifically described, it is necessary to which indicated herein is that following embodiment is only used
In invention is further explained, it should not be understood as limiting the scope of the invention, the people that is skilled in technique in the field
Member content can make some nonessential modifications and adaptations to the present invention according to the present invention.
Embodiment 1:
A kind of microbial strains can be used for silk spinning raw material oil removing, the microbial strains are clostridium perfringen, are named as
Clostridium perfringen Enterobacter sp.TY-4, the microbial strains are preserved in Chinese Typical Representative culture on June 3rd, 2018
Collection, deposit number are CCTCC NO:M2018291.
Embodiment 2:
The present embodiment is further optimized based on the above embodiments, and a kind of microbial strains are in silk spinning raw material oil removing
In application, the microbial strains be used for silk spinning raw material (or silk) oil removing, removal silk spinning raw material (or silk) in oil
Rouge.
Embodiment 3:
The present embodiment is advanced optimized on the basis of embodiment 2, further of the present invention to be better achieved
A kind of application of microbial strains in silk spinning raw material oil removing, especially use following set-up modes: the microbial strains
Be used for silk spinning raw material (or silk) oil removing when the following steps are included:
(1) preparation of oil removing microorganism；
(2) silk spinning raw material microorganism deoiling treatment；
(3) it washes: after de-oiling, taking out silk spinning raw material, wash with water；
(4) it dries: the silk spinning raw material after washing is dried；
(5) measurement of residual oil content.
Embodiment 4:
The present embodiment is advanced optimized on the basis of embodiment 3, further of the present invention to be better achieved
A kind of application of microbial strains in silk spinning raw material oil removing, especially use following set-up modes: the step (1) include with
Lower step:
(1.1) prepare seed culture medium: the beef extract of 3g/L, the peptone of 10g/L and the NaCl of 5g/L, pH are
7.0；
(1.2) microbial strains and glycerol are placed in -80 DEG C of guarantors at microorganism glycerol tube storing liquid
Hiding；
(1.3) described in (1.1) with inoculum concentration inoculation (1.2) described microorganism glycerol tube storing liquid of 1-2% (V/V)
Seed culture medium in, be placed in 37 DEG C of carry out shaking tables, 180rpm shake culture 12h obtains de-oiling microorganism；Preferably, with 1%
(V/V) inoculum concentration inoculation (1.2) described microorganism glycerol tube storing liquid is placed in 37 in the seed culture medium described in (1.1)
DEG C carry out shaking table, 180rpm shake culture 12h obtain de-oiling microorganism.
Embodiment 5:
The present embodiment is advanced optimized on the basis of embodiment 3 or 4, further for institute of the present invention is better achieved
A kind of application of the microbial strains stated in silk spinning raw material oil removing especially uses following set-up modes: the microbial strains
Proportion with glycerol is 7:3.
Embodiment 6:
The present embodiment is advanced optimized on the basis of embodiment 3 or 4 or 5, and further is that this hair is better achieved
A kind of application of the bright microbial strains in silk spinning raw material oil removing, especially uses following set-up modes: the step
(2) the following steps are included:
(2.1) preparation of microorganism de-oiling culture medium: 10g/L peptone；2.5g/L sucrose；5g/L NaCl, 1g/L
SDS；pH 7.0；
(2.2) the de-oiling microorganism being prepared with the inoculum concentration inoculation step (1.3) of 1-2% (V/V) is in step
(2.1) in the microorganism de-oiling culture medium described in, silk spinning raw material is added by bath raio 2:100, in 37 DEG C of shaking tables, 180rpm concussion training
Support 48h；Preferably, the de-oiling microorganism being prepared with the inoculum concentration inoculation step (1.3) of 1% (V/V) is in step
(2.1) in the microorganism de-oiling culture medium described in, silk spinning raw material is added by bath raio 2:100, in 37 DEG C of shaking tables, 180rpm concussion training
Support 48h.
Embodiment 7:
The present embodiment is advanced optimized on the basis of embodiment 3-6 any embodiment, and further is preferably real
A kind of application of the microbial strains now of the present invention in silk spinning raw material oil removing, especially uses following set-up modes: described
The washing concrete mode of step (3) are as follows: cleaned using the water that water temperature is 30-40 DEG C silk spinning raw material 2-4 times after de-oiling.
Embodiment 8:
The present embodiment is advanced optimized on the basis of embodiment 3-7 any embodiment, and further is preferably real
A kind of application of the microbial strains now of the present invention in silk spinning raw material oil removing, especially uses following set-up modes: described
The drying concrete mode of step (4) are as follows: the silk spinning raw material after washing is dried into 2-4h under the conditions of 110 DEG C of 100-.
Embodiment 9:
The present embodiment is advanced optimized on the basis of embodiment 3-8 any embodiment, and further is preferably real
A kind of application of the microbial strains now of the present invention in silk spinning raw material oil removing, especially uses following set-up modes: described
The measurement of residual oil content in step (5) is carried out according to soxhlet extraction methods.
Embodiment 10:
A kind of microbial strains can be used for silk spinning raw material oil removing, are named as clostridium perfringen Enterobacter
Sp.TY-4, the microbial strains are preserved in China typical culture collection center on June 3rd, 2018, and deposit number is
CCTCC NO:M2018291。
Embodiment 11
One of embodiment 10 can be used for the screening technique of the microbial strains of silk spinning raw material de-oiling, including following step
It is rapid:
(1) resulting silk spinning raw material corruption liquid 10mL will be sampled to be added in the seed culture medium of 90mL, the seed culture medium
It prepares are as follows: the beef extract of 3g/L, the peptone of 10g/L and the NaCl of 5g/L, pH 7.0.Be placed on 37 DEG C of shaking tables,
180rpm shake culture 12h, obtains bacteria suspension；
(2) it will be coated on after above-mentioned cultured bacteria suspension dilution different multiples using tributyrin as sole carbon source
On Selective agar medium agar plate, which is prepared are as follows: 1g/L ammonium nitrate, 0.5g/L potassium dihydrogen phosphate, 1.5g/L
Disodium hydrogen phosphate, 1g/L NaCl, 0.2g/L MgSO4.7H2O, 5 mL/L tributyrins are placed in 37 DEG C of culture 48h.
(3) according to the ratio of transparent circle size and colony diameter, select ratio it is larger (i.e. expression bacterial strain de-oiling ability compared with
Bacterial strain by force).
(4) by the above-mentioned stronger bacterial strain of de-oiling ability selected, (100mL) is seeded in de-oiling culture medium, de-oiling training
Feeding basigamy is made as: 10g/L peptone；2.5g/L sucrose；5g/L NaCl, 1g/L SDS； pH 7.0.It is former to add 2g silk spinning
Material, is placed in 37 DEG C of shaking tables, 180rpm shake culture 48h.
(5) silk spinning raw material de-oiling rate is measured.
After the completion of de-oiling, culture solution is discarded, the silk spinning raw material after cleaning de-oiling with the water that water temperature is 30-40 DEG C 2-4 times,
The silk spinning raw material after washing is dried into 2-4h under the conditions of 100-110 DEG C again, silk spinning raw material is placed in Soxhlet extractor, is measured
Residual oil content.
The calculation of residual oil content are as follows: the silk spinning raw material after deoiling treatment is after extracting, the increase of flask weight and silk spinning
The loss of raw material weight characterizes silk spinning raw material residual oil content.The formula that it is calculated is as follows:
Residual oil content (%)=flask variation weight/(dry weight after weight+silk spinning raw material de-oiling of flask variation) ×
100%.
(6) the minimum bacterial strain of the residual oil content of acquisition is subjected to 16S rDNA identification, is named as clostridium perfringen
Enterobacter sp.TY-4 (bacterial strain phylogenetic tree as shown in Figure 1), and bacterial strain is sent to Chinese Typical Representative culture and is protected
Hiding center, deposit number are CCTCC NO:M2018291, and preservation address is Wuhan, China Wuhan University.
Embodiment 12:
Clostridium perfringen bacterial strain described in above-described embodiment can be applied to the grease in removal silk spinning raw material (or silk).
Specific silk spinning raw material oil removing application the following steps are included:
(1) preparation of de-oiling microorganism；
(2) silk spinning raw material microorganism deoiling treatment；
(3) it washes: after de-oiling, taking out silk spinning raw material, wash with water；
(4) it dries: the silk spinning raw material after washing is dried；
(5) measurement of residual oil content.
Embodiment 13:
Clostridium perfringen bacterial strain in Application Example 10 and 11 carries out silk spinning raw material oil removing as described in Example 12
Method first prepares seed culture medium: the beef extract of 3g/L, the peptone of 10g/L, 5g/ when carrying out the preparation of de-oiling microorganism
The NaCl of L, pH 7.0；Later by the clostridium perfringen bacterial strain and glycerol at the (culture of microorganism glycerol tube storing liquid
Object (clostridium perfringen bacterial strain): glycerol=7:3), it is placed in -80 DEG C of preservations；It is inoculated in next step with the inoculum concentration of 1-2% (V/V)
Microorganism glycerol tube storing liquid (preferably, is inoculated with micro- life in seed culture medium with the inoculum concentration of 1% (V/V) or 2% (V/V)
Object glycerol tube storing liquid is in seed culture medium), 37 DEG C of carry out shaking tables are placed in, 180rpm shake culture 12h obtains the micro- life of de-oiling
Object.Remaining step is identical as embodiment 12, and details are not described herein.
Embodiment 14:
Clostridium perfringen bacterial strain in Application Example 10 and 11 carries out silk spinning raw material oil removing as described in Example 12
Method, when carrying out silk spinning raw material microorganism deoiling treatment, first preparing microorganism de-oiling culture medium: 10g/L peptone；2.5g/L
Sucrose；5g/L NaCl, 1g/L SDS；pH 7.0.
It is strictly according to the facts with the inoculum concentration of 1-2% (V/V) (preferred, with the inoculum concentration of 1% (V/V) or 2% (V/V)) inoculation later
The de-oiling microorganism being prepared described in example 13 is applied in de-oiling culture medium, silk spinning raw material is added by bath raio 2:100, is shaken in 37 DEG C
Bed, 180rpm shake culture 48h.Remaining step is identical as described in embodiment 12 and embodiment 13, and details are not described herein.
Embodiment 15:
Aeromonas bacterial strain in Application Example 10 and 11 carries out the side of silk spinning raw material oil removing as described in Example 12
Method is cleaned silk spinning raw material 3 times after de-oiling when carrying out washing process using the water that water temperature is 35 DEG C.
Embodiment 16:
Clostridium perfringen bacterial strain in Application Example 10 and 11 carries out silk spinning raw material oil removing as described in Example 12
Method is cleaned silk spinning raw material 2 times after de-oiling when carrying out washing process using the water that water temperature is 30 DEG C.
Embodiment 17:
Clostridium perfringen bacterial strain in Application Example 10 and 11 carries out silk spinning raw material oil removing as described in Example 12
Method is cleaned silk spinning raw material 4 times after de-oiling when carrying out washing process using the water that water temperature is 40 DEG C.
Embodiment 18:
Clostridium perfringen bacterial strain in Application Example 10 and 11 carries out silk spinning raw material oil removing as described in Example 12
Silk spinning raw material after washing is dried 2-4h under the conditions of 100-110 DEG C when being dried by method.
Embodiment 19:
Silk spinning raw material oil removing as described in Example 12 is carried out with the clostridium perfringen bacterial strain in embodiment 10 and 11
Method, when carrying out residual oil content measurement, method therefor is soxhlet extraction methods.
Embodiment 20:
The method that clostridium perfringen bacterial strain in Application Example 10 and 11 carries out silk spinning raw material oil removing, including following step
It is rapid:
(1) preparation of de-oiling microorganism；
(2) silk spinning raw material microorganism deoiling treatment；
(3) it washes: after de-oiling, taking out silk spinning raw material, wash with water；
(4) it dries: the silk spinning raw material after washing is dried；
In the preparation of de-oiling microorganism, seed culture medium is first prepared: the beef extract of 3g/L, the peptone of 10g/L, 5g/L's
NaCl, pH 7.0；Later by the clostridium perfringen bacterial strain and glycerol at microorganism glycerol tube storing liquid, be placed in-
80 DEG C of preservations (culture: glycerol=7:3)；In next step with the inoculum concentration of 1-2% (V/V) (preferably, with 1% (V/V) or 2%
(V/V) inoculum concentration) microbe inoculation glycerol tube storing liquid in seed culture medium, is placed in 37 DEG C of carry out shaking tables, 180rpm shake
12 h of culture are swung, de-oiling microorganism is obtained.
Silk spinning raw material microorganism deoiling treatment, first preparing microorganism de-oiling culture medium: 10g/L peptone are carried out later；
2.5g/L sucrose；5g/L NaCl, 1g/L SDS；pH 7.0；Later with the inoculum concentration of 1-2% (V/V) (preferably, with 1% (V/
V) or the inoculum concentration of 2% (V/V)) the de-oiling microorganism that is prepared as described in embodiment 3 or embodiment 4 of inoculation is in de-oiling culture
In base, silk spinning raw material is added by bath raio 2:100, in 37 DEG C of shaking tables, 180rpm shake culture 48h.
It is washed, is cleaned using the water that water temperature is 30-40 DEG C silk spinning raw material 2-4 times after de-oiling later.
It is dried after washing, the silk spinning raw material after washing is dried into 2-4h under the conditions of 100-110 DEG C.
Soxhlet type is carried out later, measures residual oil content.
Using the silk spinning raw material result according to step and condition progress deoiling treatment in embodiment 12 as experimental group, and it is another
Two groups of results as a control group, are carried out residual oil content by one group of silk spinning raw material result for not being inoculated with the microbial strains of outer preparation respectively
Calculating, the results are shown in Table 1, show experimental group residual oil content be 0.17%, far below 0.48% Residual oil of control group
Rate, it can be seen that, microbial strains provided by the present invention can effectively degrade the oil in silk spinning raw material raw material within a short period of time
Rouge, de-oiling efficiency are high.
1 clostridium perfringen de-oiling efficiency of table
The above is only presently preferred embodiments of the present invention, not does limitation in any form to the present invention, it is all according to
According to technical spirit any simple modification to the above embodiments of the invention, equivalent variations, protection of the invention is each fallen within
Within the scope of.
Claims (10)
1. a kind of microbial strains, it is characterised in that: the microbial strains are clostridium perfringen, are named as clostridium perfringen
Enterobacter sp.TY-4, the microorganism are preserved in China typical culture collection center, preservation on June 3rd, 2018
Number is CCTCC NO:M2018291.
2. a kind of application of the microbial strains as described in claim 1 in silk spinning raw material oil removing, it is characterised in that: described
Microbial strains are used for silk spinning raw material oil removing.
3. a kind of application of the microbial strains according to claim 2 in silk spinning raw material oil removing, it is characterised in that: described
Microbial strains be used for silk spinning raw material oil removing when the following steps are included:
(1) preparation of oil removing microorganism；
(2) silk spinning raw material microorganism deoiling treatment；
(3) it washes: after de-oiling, taking out silk spinning raw material, wash with water；
(4) it dries: the silk spinning raw material after washing is dried；
(5) measurement of residual oil content.
4. a kind of application of the microbial strains according to claim 3 in silk spinning raw material oil removing, it is characterised in that: described
Step (1) the following steps are included:
(1.1) seed culture medium: the beef extract of 3g/L, the peptone of 10g/L and the NaCl of 5g/L, pH 7.0 is prepared；
(1.2) microbial strains and glycerol are placed in -80 DEG C of preservations at microorganism glycerol tube storing liquid；
(1.3) seed with inoculum concentration inoculation (1.2) described microorganism glycerol tube storing liquid of 1-2% (V/V) described in (1.1)
In culture medium, 37 DEG C of carry out shaking tables are placed in, 180rpm shake culture 12h obtains de-oiling microorganism.
5. a kind of application of the microbial strains according to claim 4 in silk spinning raw material oil removing, it is characterised in that: described
The proportion of microbial strains and glycerol is 7:3.
6. a kind of application of the microbial strains according to claim 3 or 4 or 5 in silk spinning raw material oil removing, feature exist
In: the step (2) the following steps are included:
(2.1) preparation of microorganism de-oiling culture medium: 10g/L peptone；2.5g/L sucrose；5g/L NaCl, 1g/L SDS；pH
7.0；
(2.2) the de-oiling microorganism being prepared with the inoculum concentration inoculation step (1.3) of 1-2% (V/V) is in step (2.1)
In the microorganism de-oiling culture medium, silk spinning raw material is added by bath raio 2:100, in 37 DEG C of shaking tables, 180rpm shake culture
48h。
7. a kind of application of the microbial strains according to claim 6 in silk spinning raw material oil removing, it is characterised in that: described
The washing concrete mode of step (3) are as follows: cleaned using the water that water temperature is 30-40 DEG C silk spinning raw material 2-4 times after de-oiling.
8. a kind of application of the microbial strains according to claim 3 or 4 or 5 in silk spinning raw material oil removing, feature exist
In: the washing concrete mode of the step (3) are as follows: the silk spinning raw material 2-4 after de-oiling is cleaned using the water that water temperature is 30-40 DEG C
It is secondary.
9. a kind of application of the microbial strains according to claim 3 or 4 or 5 or 7 in silk spinning raw material oil removing, feature
It is: the drying concrete mode of the step (4) are as follows: the silk spinning raw material after washing is dried into 2-4h under the conditions of 100-110 DEG C.
10. a kind of application of the microbial strains according to claim 3 or 4 or 5 or 7 in silk spinning raw material oil removing, special
Sign is: the measurement of the residual oil content in the step (5) is carried out according to soxhlet extraction methods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910344834.7A CN110106110B (en) | 2019-04-26 | 2019-04-26 | Microbial strain and application thereof in silk spinning raw material oil removal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910344834.7A CN110106110B (en) | 2019-04-26 | 2019-04-26 | Microbial strain and application thereof in silk spinning raw material oil removal |
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