CN101412964A - Method for separating degumming strains for degumming silk - Google Patents
Method for separating degumming strains for degumming silk Download PDFInfo
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- CN101412964A CN101412964A CNA2008102034990A CN200810203499A CN101412964A CN 101412964 A CN101412964 A CN 101412964A CN A2008102034990 A CNA2008102034990 A CN A2008102034990A CN 200810203499 A CN200810203499 A CN 200810203499A CN 101412964 A CN101412964 A CN 101412964A
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Abstract
The invention relates to a method for separating degumming strains used for refining cocoon fibers, which comprises the following steps: (1) the preparation of a sample to be separated, which is to collect silk spinning raw material rotting liquid or a soil sample from a silk spinning factory; (2) the preliminary screening of the degumming strains, which is to adopt a pour plate method of high-protein culture medium, coat a culture medium A and a sample liquid on a pour plate for inoculation and cultivation so as to obtain a colony generating a transparent circle, inoculate the colony on a beef extract peptone inclined plane, and store the colony in a refrigerator; (3) the repeated screening of the degumming strains, which is to inoculate the strains in a conical flask which is filled with a culture medium B, and place the conical flask on a shaking bed to cultivate the strains so as to obtain to the strains acting on the cocoon fibers with the lowest raw material residual gum rate, namely the degumming strains; and (4) inoculation of the separated degumming strains on the inclined plane of a beef extract peptone culture medium, and preservation of the degumming strains in the refrigerator at a temperature of between 1 and 10 DEG C. The strains screened out by the method can directly act on the cocoon fibers for refining and degumming, and have the advantages of short refining time, less environmental pollution, and low production cost; besides, the method has the advantages of obvious effect, easy observation, and simple and convenient operation.
Description
Technical field
The invention belongs to the preparation field of strains separation, particularly relate to a kind of separation method of degumming strains for degumming silk.
Background technology
The concise purpose of silk is most of silk gum, grease and the impurity of removing in the raw material etc., gives full play to silk fiber inherent gloss and feel, keeps the original physical and mechanical properties of silk simultaneously as far as possible.Concise quality directly influence fall that can subsequent handling normally carry out, the quality of quality product and the height of production cost, the Different Effects of method for refining reaches the pollution to environment to working strength of workers, work situation.
At present, in the silk production there be method for refining commonly used: soap alkaline process and biological enzyme.The soap alkaline process is traditional method for refining, adopts chemical agent to impel the silk oil removing of coming unstuck, and speed is fast, output is high, but big to fibre-tendering, and contaminate environment is serious.Biological enzyme directly acts on the silk raw material with biological enzyme formulation exactly, makes its oil removing of coming unstuck, and can raise labour productivity, reduce environmental pollution, also little to fibre-tendering, but prior biological zymin cost height, optional kind is few, is unsuitable for factory mass production.
Summary of the invention
Technical problem to be solved by this invention provides a kind of separation method of degumming strains for degumming silk, the bacterial strain that the present invention filters out can directly act on that silk is concise to come unstuck, can effectively improve concise quality, reduce the concise time, raise labour productivity, reduce environmental pollution, reduce production costs, obvious, the easily observation, easy and simple to handle of this separation method effect.
The separation method of a kind of degumming strains for degumming silk of the present invention comprises:
(1) preparation of sample to be separated: gather the silk spinning raw material corruption and practice liquid or silk spinning factory soil sample;
(2) preliminary screening of degumming strain: adopt high protein culture medium flat plate method, culture medium A and sample liquid are coated with inoculation, cultivate on flat board, obtain producing the bacterium colony of transparent circle, with colony inoculation 37 ℃ of constant temperature culture 1d on the beef extract-peptone inclined-plane, 1～10 ℃ of preservation in refrigerator;
(3) the multiple sieve of degumming strain: above-mentioned bacterial classification inoculation in the triangular flask that substratum B is housed, is placed on the shaking table and cultivates, and obtaining acting on silk and making the raw material residual gum content is minimum bacterial classification, is the bacterial classification that comes unstuck;
(4) isolated degumming strain is inoculated into 37 ℃ of constant temperature culture 1d on the beef-protein medium inclined-plane, 1～10 ℃ of preservation in refrigerator.
Described culture medium A prescription is: Zulkovsky starch 1.0～10.0g, the high 1.0～5.0g of yeast, casein 5.0～10.0g, KH
2PO
40.1～2.0g, MgSO
40.1～1.0g, agar 15.0～20.0g, pH6.0～9.0, water 1000mL;
Described substratum B prescription is: silk raw material 25.0g, K
2HPO
40.2～5.0g, KH
2PO
41.0～10.0g, NaCl 1.0～5.0g, pH 6.0～8.0, water 1000mL;
Described beef-protein medium prescription is: extractum carnis 1.0～5.0g, and peptone 1.0～10.0g, NaCl 1.0～6.0g, agar 15.0～20.0g, pH 6.0～9.0, water 1000mL;
The inoculum size of the flat board coating inoculation in the described step (2) is 0.05～0.2mL, 30～45 ℃ of inoculation temps, incubation time 1～3d;
Shaking speed in the described step (3) is 100～300r/min, and culture temperature is 30～45 ℃, incubation time 1～5d;
Described culture medium A, substratum B, triangular flask, the beef extract-peptone inclined-plane 20～30min that in Autoclave, sterilizes respectively, Autoclave pressure is 0.7～1.2Kg/cm
2
Beneficial effect
(1) bacterial strain that filters out of the present invention can directly act on that silk is concise to come unstuck, and can effectively improve concise quality, reduces the concise time, raises labour productivity, and reduces environmental pollution, reduces production costs;
(2) obvious, the easily observation, easy and simple to handle of this separation method effect.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
(1) preparation of sample to be separated: gather the liquid after silk spinning raw material is degenerated 24h, use for separating degumming strain;
(2) preliminary screening of degumming strain: the screening method of degumming strain is with the dull and stereotyped coating of high protein partition method, with culture dish and culture medium A respectively at 1Kg/cm
2Sterilization 20min, the sterilization back is fallen dull and stereotyped, adopts the coating method inoculation, and inoculum size is 0.1mL, 37 ℃, cultivate 1～3d, obtain the bacterium colony of the degumming bacterium of five kinds of forms;
The culture medium A prescription is: Zulkovsky starch 8.0g, the high 3.0g of yeast, casein 6.0g, KH
2PO
41.0g, MgSO
40.2g, agar 20.0g, pH7.0, water 1000mL;
(3) the multiple sieve of degumming strain: will shake bottle and substratum B respectively at 1Kg/cm
2Sterilization 20min, inoculation then will inoculate the good bottle that shakes again and place and cultivate on the shaking table, 37 ℃ of temperature, rotating speed 200r/min takes out after cultivating 72h, and the residual gum content of mensuration raw material obtains the minimum bacterial strain of residual gum content,
Substratum B prescription is: silk raw material 10.0g, K
2HPO
41.0g, KH
2PO
44.0g, NaCl 2.0g, pH 7.0, water 1000mL;
(4) by the aforesaid method degumming strain of gaining the upper hand.
Embodiment 2
(1) preparation of sample to be separated: gather the silk spinning soil sample 1g of factory, add sterilized water 99mL, vibration dilution back is used for separating degumming bacterium;
(2) preliminary screening of degumming strain: the screening method of degumming strain is with the dull and stereotyped coating of high protein partition method, with culture dish and culture medium A respectively at 1Kg/cm
2Sterilization 20min, the sterilization back is fallen dull and stereotyped, adopts the coating method inoculation, and inoculum size is 0.2mL, 39 ℃, cultivates 3～5d.Obtain the bacterium colony of the degumming bacterium of four kinds of forms;
The culture medium A prescription is: Zulkovsky starch 10.0g, the high 5.0g of yeast, casein 10.0g, KH
2PO
42.0g, MgSO
40.5g, agar 18.0g, pH8.0, water 1000mL;
(3) the multiple sieve of degumming strain: will shake bottle and substratum B respectively at 1Kg/cm
2Sterilization 20min, inoculation then will inoculate the good bottle that shakes again and place and cultivate on the shaking table, 39 ℃ of temperature, rotating speed 220r/min takes out after cultivating 84h, and the residual gum content of mensuration raw material obtains the minimum bacterial strain of residual gum content;
Substratum B prescription is: silk raw material 25.0g, K
2HPO
42.0g, KH
2PO
46.0g, NaCl 1.0g, pH 8.0, water 1000mL;
(4) by the aforesaid method degumming strain of gaining the upper hand.
Claims (7)
1. the separation method of a degumming strains for degumming silk comprises:
(1) preparation of sample to be separated: gather the silk spinning raw material corruption and practice liquid or silk spinning factory soil sample;
(2) preliminary screening of degumming strain: adopt high protein culture medium flat plate method, culture medium A and sample liquid are coated with inoculation, cultivate on flat board, obtain producing the bacterium colony of transparent circle, with colony inoculation 37 ℃ of constant temperature culture 1d on the beef extract-peptone inclined-plane, 1～10 ℃ of preservation in refrigerator;
(3) the multiple sieve of degumming strain: above-mentioned bacterial classification inoculation in the triangular flask that substratum B is housed, is placed on the shaking table and cultivates, and obtaining acting on silk and making the raw material residual gum content is minimum bacterial classification, is the bacterial classification that comes unstuck;
(4) isolated degumming strain is inoculated into 37 ℃ of constant temperature culture 1d on the beef-protein medium inclined-plane, 1～10 ℃ of preservation in refrigerator.
2. the separation method of a kind of degumming strains for degumming silk according to claim 1, it is characterized in that: described culture medium A prescription is: Zulkovsky starch 1.0～10.0g, the high 1.0～5.0g of yeast, casein 5.0～10.0g, KH
2PO
40.1～2.0g, MgSO
40.1～1.0g, agar 15.0～20.0g, pH6.0～9.0, water 1000mL.
3. the separation method of a kind of degumming strains for degumming silk according to claim 1 is characterized in that: described substratum B prescription is: silk raw material 25.0g, K
2HPO
40.2～5.0g, KH
2PO
41.0～10.0g, NaCl 1.0～5.0g, pH6.0～8.0, water 1000mL.
4. the separation method of a kind of degumming strains for degumming silk according to claim 1, it is characterized in that: described beef-protein medium prescription is: extractum carnis 1.0～5.0g, peptone 1.0～10.0g, NaCl 1.0～6.0g, agar 15.0～20.0g, pH6.0～9.0, water 1000mL.
5. the separation method of a kind of degumming strains for degumming silk according to claim 1 is characterized in that: the inoculum size of the flat board coating inoculation in the described step (2) is 0.05～0.2mL, 30～45 ℃ of inoculation temps, incubation time 1～3d.
6. the separation method of a kind of degumming strains for degumming silk according to claim 1, it is characterized in that: the shaking speed in the described step (3) is 100～300r/min, culture temperature is 30～45 ℃, incubation time 1～5d.
7. the separation method of a kind of degumming strains for degumming silk according to claim 1, it is characterized in that: described culture medium A, substratum B, triangular flask, the beef extract-peptone inclined-plane 20～30min that sterilizes in Autoclave respectively, Autoclave pressure is 0.7～1.2Kg/cm
2
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008102034990A CN101412964A (en) | 2008-11-27 | 2008-11-27 | Method for separating degumming strains for degumming silk |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102034990A CN101412964A (en) | 2008-11-27 | 2008-11-27 | Method for separating degumming strains for degumming silk |
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|---|---|
| CN101412964A true CN101412964A (en) | 2009-04-22 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892184A (en) * | 2010-08-02 | 2010-11-24 | 海南省农业科学院农产品加工设计研究所 | Black pepper peel degumming strain and use thereof |
| CN102899906A (en) * | 2012-10-16 | 2013-01-30 | 华东师范大学 | Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation |
| CN107354107A (en) * | 2017-07-25 | 2017-11-17 | 四川省丝绸工程技术研究中心 | A kind of microbial strains and its application in silk degumming |
| CN107723811A (en) * | 2017-11-02 | 2018-02-23 | 大连民族大学 | The unwinding method of degumming of the fermentation of bacillus method to tussah cocoon |
-
2008
- 2008-11-27 CN CNA2008102034990A patent/CN101412964A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101892184A (en) * | 2010-08-02 | 2010-11-24 | 海南省农业科学院农产品加工设计研究所 | Black pepper peel degumming strain and use thereof |
| CN102899906A (en) * | 2012-10-16 | 2013-01-30 | 华东师范大学 | Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation |
| CN102899906B (en) * | 2012-10-16 | 2014-10-29 | 华东师范大学 | Biological preparation for spinning silk and refining and removing oil as well as preparation method and application of biological preparation |
| CN107354107A (en) * | 2017-07-25 | 2017-11-17 | 四川省丝绸工程技术研究中心 | A kind of microbial strains and its application in silk degumming |
| CN107354107B (en) * | 2017-07-25 | 2020-02-07 | 四川省丝绸工程技术研究中心 | Microbial strain and application thereof in cocoon silk degumming |
| CN107723811A (en) * | 2017-11-02 | 2018-02-23 | 大连民族大学 | The unwinding method of degumming of the fermentation of bacillus method to tussah cocoon |
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Open date: 20090422 |