CN1645113A - Rapid detecting box for vegetable nitrate content, its detecting reagent and microsampling needle - Google Patents
Rapid detecting box for vegetable nitrate content, its detecting reagent and microsampling needle Download PDFInfo
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- CN1645113A CN1645113A CN 200410065343 CN200410065343A CN1645113A CN 1645113 A CN1645113 A CN 1645113A CN 200410065343 CN200410065343 CN 200410065343 CN 200410065343 A CN200410065343 A CN 200410065343A CN 1645113 A CN1645113 A CN 1645113A
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Abstract
A test kit for fast detecting out nitrate content contained in vegetables includes body box, colourmetric plate and colourmetric card set on the body, detection reagent and sampling needle of trace amount. The reagent paste is prepared by adding white carbon in concentrated sulfuric acid-diphenylamine.
Description
Technical field
The present invention relates to a kind of nitrate salts content of vegetable speed and survey box and detectable and microsampling pin, be applicable to particularly that general layman is easy to grasp and speed that can on-the-spot quantitative determination Nitrate in Vegetable is surveyed box and detectable and microsampling pin.
Background technology
Nitrate in the vegetables is that its nitrogen nutrition of drawing from soil in growth course fails to be converted into organic principle and residual mineral compound in vivo.Excessive edible nitre hydrochlorate is unfavorable to health.The United Nations's health organization (WHO) in 1973 proposition, the amount that human body day is taken in nitrate should not surpass the 3.6mg/kg body weight.Proportionately everyone body weight 60kg counts for each person, and the nitrate of everyone absorption every day should not surpass 220mg.Because chemical nitrogen fertilizer used in a large number during vegetables produced in recent years, the Nitrate in Vegetable that China produces is generally very high, leaf vegetables especially, and its nitrate content often reaches thousands of mg/kg, become one of important hidden danger of human food's safety, paid more and more attention.
In order to control the intake of nitrate, people need a kind of method of simple fast measuring Nitrate in Vegetable.The method of measuring nitrate content is a lot, and what have is time-consuming bothersome, as phenol two yellow acid colourimetrys; The need special instruments and equipment that has, as ultraviolet spectrophotometry, sulfuric acid-salicylic acid colourimetry; Application invention disclosed patents such as increasing letter " quick detection test paper of nitrite, nitrate and detection method thereof in the vegetables " (03116663.6) is thanked to measuring the improvement of nitrate " nitric acid examination powder method " by University of Fuzhou, need earlier with zinc powder with nitrate reduction be behind the nitrite again row measure, because highly sensitive (10mg/kg) that measures, must be with the dilution in advance of dish juice, the nitrate content of vegetables juice mostly is hundreds of thousands of mg/kg, so practical operation is still comparatively loaded down with trivial details." diphenylamine sulfate method " once was used for the method for Dry crop Nitrogen Nutrition Diagnosis by introduction, by measuring the nitric acid content in the dry farming object, instruct crop fertilization, have the characteristics of rapid sensitive, but use it for the mensuration of vegetable nitrate, then have two aspect shortcomings, one of its shortcoming is the same with nitric acid examination powder method, sensitivity is too high, and color range is 1-10mg/kg, and vegetables juice is difficult to distinguish the height of its nitrate content without dilution; Two of shortcoming is that agents useful for same has severe corrosive, and the layman uses easily and has an accident.
Summary of the invention
The objective of the invention is to: provide nitrate salts content of vegetable speed to survey box and detectable and microsampling pin, simplify trace routine, improve detection speed, be convenient to on-site measurement, be applicable to that the layman operates.
Technical solution of the present invention is: according to Nitrate in Vegetable, and the concrete condition of the edible grade scale of vegetables safety of hygiene department's proposition, according to " sulfuric acid-diphenylamine method " is the basis, proterties to reagent is improved, and chromogenic reagent sulfuric acid-diphenylamine is changed into body of paste by liquid; And detectable, microsampling pin, colorimetric card and grade scale color board concentrated in the box, this speed is surveyed box and is included box body, and color board and colorimetric card are installed on box body, placement detectable and microsampling pin in box the chamber in.
The detectable that speed is surveyed in the box is clear, colorless or light yellow paste, include the concentrated sulphuric acid, diphenylamine and in the concentrated sulphuric acid stable solid material, this solid material is white carbon, barium sulphate or aluminium sulphate.Contain proportion 1.84 sulfuric acid 75-85 gram as every 100g reagent, white carbon 15-25 gram, diphenylamine 0.3-0.5 gram.Reagent is contained in the plastic tube, and the mouth of pipe has lid, and is salable.
Speed is surveyed in the box three sampling probes, and the front end of sampling probe is hydrophilic plane, and the sampling of dish juice is relevant with the hydrophilic plane and the surface tension thereof of sampling probe.Wherein the diameter of No. 1 sampling probe sample face is 1mm, and the sampling face diameter of No. 2 sampling probes is 2mm, and No. 3 sampling face diameter is 3mm.Can dip in the dish juice of getting about 0.75mg, 1.5mg, 6mg respectively.
Speed is surveyed color board and colorimetric card is installed in the box, color board is the cardboard of surface coated polyethylene film, can directly on color board, show colorimetric during mensuration, little orchid, shallow orchid, deep blue and blue black four mark looks are arranged on the colorimetric card, represent the colour developing result of different nitrate contents.Different according to sampling, can measure vegetables NO
3-100-6400mg/kg varying level.
The present invention adopts a kind of semiquantitative microsampling method, saves sample and need dilute the back and measure tiredly, is convenient to on-site measurement, is applicable to that the layman operates, and mensuration can be finished in tens of seconds, and is fast simple.
Description of drawings
Fig. 1 surveys the box synoptic diagram for nitrate speed of the present invention
Among the figure: 1 box body, 2 color boards, 3 colorimetric cards, 4 box chambeies, 5 detectable, 6 microsampling pins, 7 standard color ranges, 8 sampling spots, 9 compare color dot
Embodiment
As shown in the figure, this speed is surveyed box and is included box body 1, and color board 2 and colorimetric card 3 are installed on box body 1, places detectable 5 and several microsampling pins 6 in the box chamber 4; The standard of arranging on colorimetric card 3 color range 7 is established one group of sampling spot 8 and one group than color dot 9 on color board 2.
The step of using vegetable nitrate speed survey box mensuration Nitrate in Vegetable is as follows:
1, pushes vegetable sample to be measured, get a juice and put on the sampling spot of color board.
2, on the ratio color dot of color board, add a sulfuric acid-diphenylamine paste detectable that is about 1cm, about 10 microlitres of volume.
3, with No. 2 sampling probes dip in get dish juice after, in sulfuric acid-diphenylamine detectable, fully stir, and with colorimetric card on standard color range colorimetric, i.e. NO in its vegetables of decidable
3Contents level.If color is shallow excessively, the available No. 3 sampling probes colorimetric of resampling.If the color is too dark, the available No. 1 sampling probe colorimetric of resampling.After colorimetric finished, available thieving paper was dried the back with the color board surface and is continued to use.
Claims (10)
1. nitrate salts content of vegetable speed is surveyed box, includes box body (1), it is characterized in that: place detectable (5) and microsampling pin (6) in box chamber (4).
2. nitrate salts content of vegetable speed according to claim 1 is surveyed box, it is characterized in that: this speed is surveyed the box body (1) of box and is gone up installation color board (2) and colorimetric card (3).
3. nitrate salts content of vegetable speed according to claim 1 is surveyed box, and it is characterized in that: colorimetric card (3) is made up of surperficial acid resisting material, and the standard color range (7) black from little orchid to orchid arranged.
4. nitrate salts content of vegetable according to claim 1 speed is surveyed box, it is characterized in that: color board (2) surface is made up of acid resisting material, establishes one group of sampling spot (8) and one group than color dot (9).
5. the detectable that nitrate salts content of vegetable according to claim 1 speed is surveyed is characterized in that: this detectable comprises the concentrated sulphuric acid, diphenylamine and stable solid material in the concentrated sulphuric acid.
6. the detectable that nitrate salts content of vegetable speed according to claim 5 is surveyed, it is characterized in that: solid material is white carbon, barium sulphate or aluminium sulphate.
7. the detectable that nitrate salts content of vegetable speed according to claim 5 is surveyed, it is characterized in that: the solid material addition is the 1/2-1/4 of the concentrated sulphuric acid.
8. the detectable that nitrate salts content of vegetable speed according to claim 5 is surveyed, it is characterized in that: the diphenylamine addition is the 0.1%-1% of the concentrated sulphuric acid.
9. the microsampling pin that nitrate salts content of vegetable speed according to claim 1 is surveyed, it is characterized in that: the microsampling pin is that a kind of front end is the acid resisting material on hydrophilic plane, and the diameter on its hydrophilic plane is 1-5mm.
10. the microsampling pin that nitrate salts content of vegetable speed according to claim 9 is surveyed, it is characterized in that: three sampling probes are arranged, wherein the diameter of No. 1 sampling probe sampling face is 1mm, the sampling face diameter of No. 2 sampling probes is 2mm, No. 3 sampling probe sampling face diameter is 3mm, can dip in the dish juice of getting about 0.75mg, 1.5mg, 6mg respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100653432A CN100395539C (en) | 2004-11-26 | 2004-11-26 | Rapid detecting box for vegetable nitrate content, its detecting reagent and microsampling needle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100653432A CN100395539C (en) | 2004-11-26 | 2004-11-26 | Rapid detecting box for vegetable nitrate content, its detecting reagent and microsampling needle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1645113A true CN1645113A (en) | 2005-07-27 |
| CN100395539C CN100395539C (en) | 2008-06-18 |
Family
ID=34868863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100653432A Expired - Fee Related CN100395539C (en) | 2004-11-26 | 2004-11-26 | Rapid detecting box for vegetable nitrate content, its detecting reagent and microsampling needle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100395539C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965180A (en) * | 2020-08-28 | 2020-11-20 | 中国石油化工股份有限公司 | Quantitative detection method for alkyl nitrate in diesel oil |
| CN114088896A (en) * | 2022-01-20 | 2022-02-25 | 深圳市汇知科技有限公司 | Detection device and detection method for heavy metals in agricultural products |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3405912A1 (en) * | 1984-02-18 | 1985-08-22 | Merck Patent Gmbh, 6100 Darmstadt | METHOD AND MEANS FOR DETERMINING NITRATIONS |
| US5296380A (en) * | 1992-09-03 | 1994-03-22 | Israel Institute For Biological Research | Method and kit for detecting explosives |
| DE19600521C2 (en) * | 1995-01-10 | 1998-07-23 | Christian Stoehr | Device for determining the nitrate content of food |
| CN1320816A (en) * | 2000-04-24 | 2001-11-07 | 攀枝花市卫生防疫站 | Rapid detection method of nitrite in food |
| KR20040090356A (en) * | 2003-04-17 | 2004-10-22 | (주) 휴마스 | The Simplied method of analysis for Nitrate and Nitrite |
| CN1445531A (en) * | 2003-04-26 | 2003-10-01 | 福州大学 | Test paper for fast testing nitrite, azotate in vegetables and its testing method |
-
2004
- 2004-11-26 CN CNB2004100653432A patent/CN100395539C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965180A (en) * | 2020-08-28 | 2020-11-20 | 中国石油化工股份有限公司 | Quantitative detection method for alkyl nitrate in diesel oil |
| CN114088896A (en) * | 2022-01-20 | 2022-02-25 | 深圳市汇知科技有限公司 | Detection device and detection method for heavy metals in agricultural products |
| CN114088896B (en) * | 2022-01-20 | 2022-04-05 | 深圳市汇知科技有限公司 | Detection device and detection method for heavy metals in agricultural products |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100395539C (en) | 2008-06-18 |
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| C06 | Publication | ||
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| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080618 Termination date: 20091228 |