CN101672789A - Quick detection method for nitrate nitrogen on vegetables and quick detection box thereof - Google Patents
Quick detection method for nitrate nitrogen on vegetables and quick detection box thereof Download PDFInfo
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- CN101672789A CN101672789A CN200810042909A CN200810042909A CN101672789A CN 101672789 A CN101672789 A CN 101672789A CN 200810042909 A CN200810042909 A CN 200810042909A CN 200810042909 A CN200810042909 A CN 200810042909A CN 101672789 A CN101672789 A CN 101672789A
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Abstract
The invention belongs to measurement of nitrogen nutrient components of crops. A quick detection method for nitrate nitrogen on vegetables is characterized by comprising the following steps: placing apetiole base of a vegetable leaf between two glass sheets, extruding the two glass sheets to make juice in the vegetable petiole base flow into a depression of a white porcelain board, dropping two drops of diphenylamine-sulfuric acid developing agent into the depression of the white porcelain board storing the vegetable juice by a dropper, standing the white porcelain board for 1 minutes, and then comparing the blue developed in the depression of the white porcelain board with standard color levels of five standard colors of micro blue, light blue, deep blue, black blue and ink blue containing 200 mg/kg, 400 mg/kg, 800 mg/kg, 1,200 mg/kg and 1,600 mg/kg of nitrate nitrogen so as to determine the content range of a nitrate nitrogen sample.
Description
Technical field:
The present invention relates to a kind of mensuration of crop nitrogen nutrition composition, particularly relate to a kind of nitrate nitrogen on vegetables fast determining method and speed survey box thereof.
Background technology:
Vegetables are to keep the required a kind of food of people's life activity nutrition, and with the continuous development of science and technology, and vegetables produce also from extensive field planting and promote facility cultivation for intensive cultivation, and the quality and yield of vegetables is greatly improved.In the growth course of vegetables, dystrophia mainly is the shortage and the surplus of nitrogen element, and still lack dystrophic method of early diagnosis in the planting technology of gardening at present, often diagnose from the mode of appearance of crop, even by dystrophia to the infringement of crop show as outward appearance unusual after, if can accurately diagnose and in time sue and labour to the ill, also will cause 10～20% production loss.So the early nutrition diagnostic techniques is the dystrophic key of control fast and accurately.
Owing to make nutrient situation in the object and be soil nutrient supply, crop concentrated expression to nutrient demand and its receptivity, so by nutrient situation detects in the object to doing, just can know the current nutrition condition of crop, and decide the scheme of fertilising as certificate.Therefore in view of nitrogen occupies the top priority in the crop alimentary element, weigh the diagnostic techniques that crop alimentary becomes with the plant total nitrogen content and successfully be applied to abroad instruct in the agricultural procedure.But full nitrogen analysis complex operation, workload are big, are not easy to apply.Discover that crop when its total nitrogen content surpasses a certain threshold value, begins to accumulate nitrate nitrogen (NO in the root of plant, stem, the leaf in growth course
3-N), early stage in plant growth, particularly when nitrogen supply when waning to abundant transition, nitrate nitrogen is as non-metabolite, is present in a kind of half deposit state and does in the object, in case when crop has slight nitrogen stress, the full nitrogen storehouse of crop does not also have significant change, and marked change has taken place in its nitrate nitrogen storehouse, if when surpassing the crop demand for nitrogen, also there is by a relatively large margin increase in the nitre attitude storehouse of crop than full nitrogen storehouse.Therefore,, more sensitiveer than full nitrogen, in time reflect current soil for the nitrogen state and make nitrogen nutrition level in the object with the concentration of the free nitrate nitrogen that contains in the crop as the nitrogen nutrition index, and instruct fertilising in view of the above.
The assay method of nitrate nitrogen has colourimetry, polarography, catalysis spectrophotometric method, ultraviolet spectrophotometry, reflectometer method, nitrate electrode method at present, these methods all need special instrument and equipment and professional testing staff, not only during check fee, the mensuration of single sample needs 4～5 hours, the testing expense height, and be only limited in the indoor mensuration of Specialty Experiment, be unsuitable for the real estate fast measuring of vegetables production and instruct the needs that apply fertilizer timely.
Summary of the invention:
The object of the present invention is to provide a kind of nitrate nitrogen on vegetables fast determining method and speed thereof to survey box, solve with fast, simple, be applicable to the plant nitrate nitrogen assay method that the vegetables field produces and the technical matters of equipment.
The technical scheme that the present invention solves the problems of the technologies described above is:
A kind of nitrate nitrogen on vegetables fast determining method, it is characterized in that: the petiole base of vegetables blade is placed between two blocks of glass sheet, pushing this two glass sheet flows in the depression of white plaque the interior juice of vegetables petiole base wherein, in the depression of the white plaque of contain vegetables juice, splash into 2 of diphenylamine-sulfuric acid developer with dropper, after leaving standstill 1 minute, to show blue in this white plaque depression and contain 200mg/kg, 400mg/kg, 800mg/kg, 1200mg/kg, little indigo plant of 1600mg/kg nitrate nitrogen, light blue, dark blue, blue-black, the standard color range of five marks of blue black look compares, and determines the content range of this sample nitrate nitrogen.
A kind of speed of above-mentioned nitrate nitrogen on vegetables fast determining method of using is surveyed box, comprise box body, have white plaque, glass sheet, dropper, developer bottle, the standard color comparison card of depression, it is characterized in that: the developer in this developer bottle is for being dissolved in the diphenylamine-sulphate reagent of proportional arrangement in the 100ml concentrated sulphuric acid by the 1g diphenylamine; This standard color comparison card is little blueness of containing the 200mg/kg nitrate nitrogen, contain the 400mg/kg nitrate nitrogen light blue, the mazarine that contains the 800mg/kg nitrate nitrogen, contain the black-and-blue of 1200mg/kg nitrate nitrogen and contain multicolored rank standard color card of the blue black look of 1600mg/kg nitrate nitrogen.
Beneficial effect of the present invention:
1. in the different phase of vegetable growth, use this nitrate nitrogen speed survey technology can obtain nitrogen shape total value diagnostic result in the body in good time, can instruct economically according to diagnostic result and to topdress, the output of in time avoiding causing because of fertilizer deficiency descends, and retrieves economic losses.
2. it is simple and reasonable that speed provided by the present invention is surveyed box, stores easy to carryly, is fit to field on-site sampling detection;
3. the detection method that the present invention set up is easy, and sample process only needs juice is got in the petiole extruding, need not heating and extracts, filters complex operations processes such as colorimetric, does not also need professional and technical personnel and special instruments and equipment;
4. detection speed is fast, and whole process only needs 3～5 minutes, detects with low costly, is easy to popularization;
5. color development is stable, can accurately judge nitrogen nutritional status in the vegetables body;
6. according to the recommendation tables of rationally topdressing of vegetables nitrogen situation formulation, data are reliable, and good reproducibility can in time be that vegetables are replenished nutrient.
Embodiment:
The present invention should carry out at fine day 9:00～10:00 in the morning, and vegetable plant strain internal metabolism level is in dynamic balance state in this time period, and the nitrate nitrogen that stores in the body can reflect the relativeness between Nutrient Absorption and the assimilation.The sample section of petiole base clip 2～3cm length of the leaf that launches fully at the vegetable plant strain middle part, survey rapidly and take out glass sheet in the box, above-mentioned sample section is placed between two blocks of glass sheet, firmly push this two blocks of glass sheet with hand, juice in the petiole base sample section that is clipped in is wherein extruded, making it to flow into speed surveys in the box in the depression on the white plaque, with speed survey dropper in the box survey rapidly draw diphenylamine-sulphate reagent in the developer bottle in the box after, splash into 2 diphenylamine-sulphate reagents in the juice in the white plaque depression, left standstill 1 minute, juice in the white plaque depression will stably form blue quinoid biphenylamine, survey little indigo plant of the standard color comparison card in the box then with this blue colored intensity and speed, light blue, dark blue, blue-black, five mark color ratios of five standard color ranges of blue black, the colour developing of judging sample is in that color range of standard color comparison card, and (little indigo plant is 200mg/kg to the nitrate nitrogen content of corresponding each color range, light blue is 400mg/kg, dark blue is 800mg/kg, blue-black is 1200mg/kg, black blue be 1600mg/kg) just can diagnose out the nitrogen nutrition levels of vegetables.At last, contrast different vegetable nitrate nitrogen speed is surveyed the fertilising consumption of diagnostic fertilization reference table 1～3 recommendation to the vegetables application in time.
Table 1 cucumber petiole nitrate nitrogen speed is surveyed diagnostic fertilization with reference to (being used for standard color range method)
Table 2 tomato petiole nitrate nitrogen speed is surveyed diagnostic fertilization with reference to (being used for standard color range method)
Table 3 wild cabbage petiole nitrate nitrogen speed is surveyed diagnostic fertilization with reference to (being used for standard color range method)
Diphenylamine-sulphate reagent that above-mentioned speed is surveyed in the interior developer bottle of box is prepared in following ratio:
Diphenylamine 1g;
The concentrated sulphuric acid 100ml of percentage concentration 98%.
Be loaded on after the dissolving in the developer bottle.
The speed of above-mentioned nitrate nitrogen on vegetables was surveyed process generally only time-consuming 3～5 minutes, tested the lid that closes that finishes, and just can carry easily.Speed is surveyed in the box and is preferably disposed blade or little scissors again, more helps the collection of petiole base sample.
Claims (2)
1, a kind of nitrate nitrogen on vegetables fast determining method, it is characterized in that: the petiole base of vegetables blade is placed between two blocks of glass sheet, pushing this two glass sheet flows in the depression of white plaque the interior juice of vegetables petiole base wherein, in the depression of the white plaque of contain vegetables juice, splash into 2 of diphenylamine-sulfuric acid developer with dropper, after leaving standstill 1 minute, to show blue in this white plaque depression and contain 200mg/kg, 400mg/kg, 800mg/kg, 1200mg/kg, little indigo plant of 1600mg/kg nitrate nitrogen, light blue, dark blue, blue-black, the standard color range of five marks of blue black look compares, and determines the content range of this sample nitrate nitrogen.
2, a kind of speed of above-mentioned nitrate nitrogen on vegetables fast determining method of using is surveyed box, comprise box body, have white plaque, glass sheet, dropper, developer bottle, the standard color comparison card of depression, it is characterized in that: the developer in this developer bottle is for being dissolved in the diphenylamine-sulphate reagent of proportional arrangement in the 100ml concentrated sulphuric acid by the 1g diphenylamine; This standard color comparison card is little blueness of containing the 200mg/kg nitrate nitrogen, contain the 400mg/kg nitrate nitrogen light blue, the mazarine that contains the 800mg/kg nitrate nitrogen, contain the black-and-blue of 1200mg/kg nitrate nitrogen and contain multicolored rank standard color card of the black and blue color of 1600mg/kg nitrate nitrogen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN200810042909A CN101672789A (en) | 2008-09-12 | 2008-09-12 | Quick detection method for nitrate nitrogen on vegetables and quick detection box thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810042909A CN101672789A (en) | 2008-09-12 | 2008-09-12 | Quick detection method for nitrate nitrogen on vegetables and quick detection box thereof |
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| CN101672789A true CN101672789A (en) | 2010-03-17 |
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| CN200810042909A Pending CN101672789A (en) | 2008-09-12 | 2008-09-12 | Quick detection method for nitrate nitrogen on vegetables and quick detection box thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267157A (en) * | 2014-10-21 | 2015-01-07 | 中国科学院新疆生态与地理研究所 | Method for rapidly testing and judging ricinus communis phosphorus nutrition by utilizing tissue juice |
| CN104472090A (en) * | 2014-11-20 | 2015-04-01 | 中国科学院新疆生态与地理研究所 | Method for rapidly testing tissue juice to judge nitrogen nutrition of ricinus communis |
| CN105758854A (en) * | 2016-03-07 | 2016-07-13 | 东莞翔液体肥料有限公司 | Plant nutrient rapid measurement method and rapid measurement kit |
-
2008
- 2008-09-12 CN CN200810042909A patent/CN101672789A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104267157A (en) * | 2014-10-21 | 2015-01-07 | 中国科学院新疆生态与地理研究所 | Method for rapidly testing and judging ricinus communis phosphorus nutrition by utilizing tissue juice |
| CN104472090A (en) * | 2014-11-20 | 2015-04-01 | 中国科学院新疆生态与地理研究所 | Method for rapidly testing tissue juice to judge nitrogen nutrition of ricinus communis |
| CN105758854A (en) * | 2016-03-07 | 2016-07-13 | 东莞翔液体肥料有限公司 | Plant nutrient rapid measurement method and rapid measurement kit |
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Open date: 20100317 |