CN1633875A - Duck's egg membrane extraction preparation method - Google Patents
Duck's egg membrane extraction preparation method Download PDFInfo
- Publication number
- CN1633875A CN1633875A CNA2004100235253A CN200410023525A CN1633875A CN 1633875 A CN1633875 A CN 1633875A CN A2004100235253 A CNA2004100235253 A CN A2004100235253A CN 200410023525 A CN200410023525 A CN 200410023525A CN 1633875 A CN1633875 A CN 1633875A
- Authority
- CN
- China
- Prior art keywords
- egg
- extract
- preparation
- hydrolysis
- duck
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000272525 Anas platyrhynchos Species 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 238000000409 membrane extraction Methods 0.000 title 1
- 230000007062 hydrolysis Effects 0.000 claims abstract description 16
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 16
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 208000007107 Stomach Ulcer Diseases 0.000 claims abstract description 6
- 239000003513 alkali Substances 0.000 claims abstract description 6
- 201000005917 gastric ulcer Diseases 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 3
- 230000000694 effects Effects 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 238000006386 neutralization reaction Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 6
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 229920001184 polypeptide Polymers 0.000 abstract description 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 3
- 239000000413 hydrolysate Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 abstract description 2
- 238000007865 diluting Methods 0.000 abstract 1
- 150000003384 small molecules Chemical class 0.000 abstract 1
- 235000013601 eggs Nutrition 0.000 description 29
- 102000002322 Egg Proteins Human genes 0.000 description 8
- 108010000912 Egg Proteins Proteins 0.000 description 8
- 208000025865 Ulcer Diseases 0.000 description 5
- 210000004379 membrane Anatomy 0.000 description 5
- 231100000397 ulcer Toxicity 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 4
- 210000003278 egg shell Anatomy 0.000 description 4
- 210000000969 egg white Anatomy 0.000 description 4
- 235000014103 egg white Nutrition 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 206010052428 Wound Diseases 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 210000000795 conjunctiva Anatomy 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 208000006673 asthma Diseases 0.000 description 2
- 210000004087 cornea Anatomy 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940012466 egg shell membrane Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 206010002953 Aphonia Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 208000004930 Fatty Liver Diseases 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 206010019708 Hepatic steatosis Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010020850 Hyperthyroidism Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 208000023652 chronic gastritis Diseases 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000001804 debridement Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 210000003717 douglas' pouch Anatomy 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 210000001156 gastric mucosa Anatomy 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 229960001520 ranitidine hydrochloride Drugs 0.000 description 1
- GGWBHVILAJZWKJ-KJEVSKRMSA-N ranitidine hydrochloride Chemical compound [H+].[Cl-].[O-][N+](=O)\C=C(/NC)NCCSCC1=CC=C(CN(C)C)O1 GGWBHVILAJZWKJ-KJEVSKRMSA-N 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 231100000240 steatosis hepatitis Toxicity 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
Abstract
The invention relates to a method for extracting small molecule polypeptides for medicament preparation, which comprises carrying out alkali hydrolysis to the duck's egg membrane, removing residue after the completion of hydrolysis, diluting the solution for use as medicament directly, which has the function for treating stomach ulcer. The hydrolysate can also be neutralized for alkali expelling, so as to prepare concentrated liquid or dried product after concentration.
Description
Technical field:
The present invention relates to a kind of method that material such as micromolecule polypeptide is used to prepare medicine of from membrane of duck egg, extracting.
Background technology
The egg film contains nutriments such as several amino acids, polypeptide.Burn can be used for the treatment of, ulcer can be cured.
Eggshell membrane can be controlled slight burn, is because of the viscosity of eggshell membrane attaching is very strong, not easily separated, can shield to the surface of a wound, generally need not after the use to change dressings, until wound healing again.The method of making is: select new fresh hen egg for use, with clear water shell is cleaned, be soaked in 75% alcohol and sterilized 15 minutes, break egg then, pour out egg white and yolk, with syringe water is injected between eggshell and the egg film, make its separation.After the egg film stripped out, with clear water that egg white rinsing residual on the film is clean, place in 95% alcohol.After to burn surface of a wound debridement, disinfection, standby egg film close is attached at the surface of a wound gets final product.
Fresh hen egg film therapy: with 75% alcohol disinfecting eggshell, break eggshell gently into pieces with the sterilization tweezers then, and with the sterilization pincet scrambled egg sheet is removed, expose the egg film, draw back palpebra inferior with drag hook, after dripping surface anaesthetic, center with the egg diaphragm is placed on the cornea, and the edge of film slides into the conjunctival cul-de-sac bottom, and papebral conjunctiva is not contacted with bulbar conjunctiva, cornea, wrapping is fixing, the egg film need be placed intraocular 7 to 12 days, changed a fresh-laid egg film in average 3 days, to improve curative effect, when changing the egg film, must be the egg white face towards bulbar conjunctiva.
The method of egg film treatment ulcer: open quarter from the centre, the yellow and white are poured out, to be close to some broad thin transparent film of one deck on the eggshell tearing it down gently with tweezers, piecemeal will have the side of egg white to be attached on the ulcer surface of skin, and several descend of blowing gently dries up back egg film with it and just be attached on the skin, after 24 hours, wash off with clear water and to dry skin surface, continue to stick the egg film, until the ulcer surface healing, a lot of patients of this way are good with the back effect.
In addition, get dry egg film (preferably chick hatches the egg film behind the shell) 2～5 grams, decoction, moistening lung is only chanted, and controls diseases such as asthma, aphonia, dizziness, order screen, also has report to extract and makes cosmetics, is used to brighten and improve looks.
Above-mentioned tradition method among the people shows medically have good effect on the egg film, but it all is to use with original egg film, uses inconvenience, and this method can not the most effectively provide using dosage simultaneously, can't accurately determine use amount.
Summary of the invention:
The objective of the invention is to propose the effective ingredient of membrane of duck egg, be used for treatment of diseases such as gastric ulcer.
Method of the present invention is at first membrane of duck egg to be carried out basic hydrolysis, removes residue after hydrolysis is finished, and the solution dilution directly as drug use, is gastric ulcer and has the obvious treatment effect.Also the hydrolysate neutralization that makes can be removed alkali, concentrate and make concentrate or dry product use.
Described basic hydrolysis ratio be egg film and alkali by 1: 0.7-1.4.
Described hydrolysis time is 150-200 hour.
Described dilution ratio is 100-800 times.
Described extract can be made oral liquid, spray, tablet, capsule, injection.
Through facts have proved, this extract is suitable for following illness: gastric ulcer, gastric mucosa, chronic gastritis, cancer, hypertension, rheumatoid arthritis, the proliferation of mammary gland, hepatitis B, fatty liver, diabetes, hyperthyroidism, barrier, leukaemia, decrease of platelet, asthma, climacteric metancholia again.Has raising immunity, antiviral, analgesic activity.Directly with 100 times of this extract dilutions, to the pharmacodynamics test of the experimental gastric ulcer of big white mouse, can significantly dwindle the ulcer volume, compare with ranitidine hydrochloride capsules, the two drug effect is approaching.
The specific embodiment:
Concrete steps are as follows:
1, handle: FRESH DUCK EGGS film water is rinsed well, rub do after, the chopping of egg film is standby.
2, hydrolysis: get egg film 5 grams, pour concentration 5% NaOH 100ml into, the hydrolysis 180 hours that is mixed was stirred once hydrolysis temperature 20-45 in per 12 hours.
Annex 1 is the report of extract of the present invention (annex name stomach health peptide) pharmaceutical test.
Attached 2 is membrane of duck egg basic hydrolysis extract molecular weight mass spectral analysis report.
Claims (6)
1, a kind of membrane of duck egg preparation method of extract is characterized in that at first the egg film being carried out basic hydrolysis, removes residue after hydrolysis is finished, and the solution dilution directly as drug use, is gastric ulcer and has the obvious treatment effect.
2, membrane of duck egg preparation method of extract according to claim 1 is characterized in that above-mentioned basic hydrolysis thing, and neutralization removes alkali, concentrates and makes concentrate or dry product.
3, membrane of duck egg preparation method of extract according to claim 1, it is characterized in that described basic hydrolysis ratio be egg film and alkali by 1: 0.7-1.4.
4,, it is characterized in that described hydrolysis time is 150-200 hour according to the described egg film of claim 1 preparation method of extract.
5,, it is characterized in that described hydrolysis time is 180 hours according to the described egg film of claim 4 preparation method of extract.
6, egg film preparation method of extract according to claim 1 is characterized in that described extract can make oral liquid, spray, tablet, capsule, injection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100235253A CN1633875A (en) | 2004-01-13 | 2004-01-13 | Duck's egg membrane extraction preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2004100235253A CN1633875A (en) | 2004-01-13 | 2004-01-13 | Duck's egg membrane extraction preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1633875A true CN1633875A (en) | 2005-07-06 |
Family
ID=34845692
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004100235253A Pending CN1633875A (en) | 2004-01-13 | 2004-01-13 | Duck's egg membrane extraction preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1633875A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104292364A (en) * | 2014-09-30 | 2015-01-21 | 浙江大学 | A method for extracting biologically active substances from eggshell membranes |
-
2004
- 2004-01-13 CN CNA2004100235253A patent/CN1633875A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104292364A (en) * | 2014-09-30 | 2015-01-21 | 浙江大学 | A method for extracting biologically active substances from eggshell membranes |
| CN104292364B (en) * | 2014-09-30 | 2016-08-03 | 浙江大学 | A kind of method extracting bioactive substance from egg shell membrane |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20050706 |