CN1632099A - Vinegar containing organic selenium from aspergillus niger and method for preparing same - Google Patents
Vinegar containing organic selenium from aspergillus niger and method for preparing same Download PDFInfo
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- CN1632099A CN1632099A CN 200410011259 CN200410011259A CN1632099A CN 1632099 A CN1632099 A CN 1632099A CN 200410011259 CN200410011259 CN 200410011259 CN 200410011259 A CN200410011259 A CN 200410011259A CN 1632099 A CN1632099 A CN 1632099A
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Abstract
The invention includes vinegar of molds organic selenium and its preparation belonging to the technical field of condiment and health products organic selenium is prepared by molds, by the content of Se in vinegar is 0.1-10mg/kg, it is prepared by the steps including cultivating molds in culture medium of liquid of bean sprouts containing sodium selenite, accumulating cells of molds, washing sodium selenite absorbed onto the cell wall, cracking the cell of germ and so on to prepare the vinegar containing organic Se which can be absorbed by human body. Using rice bran and rice hull as the raw material, by the course including shattering, boiling to make the general vinegar, then add the organic selenium into vinegar to prepare the vinegar containing organic Se which can be absorbed by human body. The method has increased the conversion efficiency of organic selenium, shortened the productive period, decreased the cost, can accurately control the content of organic selenium in vinegar, benefiting for the production and introduction of vinegar for supplementing Se. The vinegar made from molds organic selenium can be easily absorbed by body, as a continuous way for supplementing Se, it can be mainly applied in food, condiment etc., meeting the market's requirement.
Description
Technical field
The invention belongs to food, seasonings and health food technology field, relate to a kind of preparation method who adds organoselenium vinegar.
Technical background
At present, the seasonings of production and selling faces new opportunity to develop.Seasonings such as vinegar, soy sauce, sauce class development is very fast, scale in the structural adjustment of product, improves grade, and has novelty to break through, and will become the main flow of following flavoring industry development.Vinegar is with a long history in China as a kind of edible flavouring, especially when the various dish of the cooking, is absolutely necessary.The physiological hygiene function that vinegar has, the sterilization detoxification is paid attention to by people.
Selenium is present in the proteolytic enzyme of the multiple function of human body; participate in the metabolism of body endoperoxide and iodine; have and remove the effect that immunity is regulated in free radical, protection liver, anti-ageing beauty treatment etc., treatment of diseases such as hepatitis, sacroiliitis, cardiovascular diseases are had significant promotion restitution.Prevention now and treatment are because the disease main method that scarce selenium causes is manually to mend selenium.Inorganic selenium toxicity is big, absorption is less.Have only inorganic selenium is converted into physiologically active and the specific absorption that organoselenium could improve selenium, reduce its toxicity simultaneously, just have general edible and health care and be worth.
It is 96109725.6 patented product that present organoselenium vinegar has application number, is to be starting raw material with Chinese sorghum, corn and cereal, the conventional vinegar of brewageing through boiling, koji, solid state fermentation add organoselenium, vitamin A, vitamin-E and selenium-rich vinegar.The organoselenium that adds be in add after the fermentation or make conventional vinegar after add.Organoselenium carries out plant absorbing and changes in inorganic selenium salt and soybean and barley, wheat.Reality according to the Plant Transformation organoselenium, transform the yielding poorly of organoselenium, the cycle is long, cost is high, and the selenium content of its Plant Transformation is not easy control, moreover the soybean, barley, the wheat that add mainly are undressed protein and farinose form, addition just is restricted like this, and organic selenium content wherein will still less can not satisfy the needs that human body replenishes organoselenium.
Summary of the invention
The technical problem to be solved in the present invention is the shortcoming that overcomes the selenium-enriched vinegar of background technology, adopted the organoselenium of microbial transformation, improved organoselenium transformation efficiency, shortened the cycle of producing, the cost that greatly reduces organoselenium, the production and the popularization that help mending selenium vinegar.Nutritive substances such as rich in proteins, amino acid, nucleic acid, VITAMIN, polysaccharide in the extracellular microbial can significantly improve the quality of vinegar.
The invention provides a kind of selenium-rich vinegar, is to add organoselenium in the common vinegar of making, and it is characterized in that organoselenium is the organoselenium that derives from aspergillus niger, and the content of organoselenium is 0.1～10mg/kg in the vinegar.
The invention provides a kind of preparation method who derives from aspergillus niger organoselenium vinegar that added, can be divided into the preparation of organoselenium, the preparation and the organoselenium of vinegar adds three steps in the vinegar to, strengthen the organoselenium in the vinegar, make conventional vinegar become the health vinegar that contains organoselenium.The preparation process of vinegar is identical substantially with background technology with the processing step that adds organoselenium, is starting raw material with high-quality jowar, corn, cereal, makes conventional vinegar through pulverizing, boiling, koji, solid state fermentation; In the vinegar of making, add organoselenium.The invention is characterized in:
The organoselenium that adds in the vinegar prepares by using aspergillus niger conversion very widely in food and the seasonings industry;
The preparation of organoselenium comprises the preparation that contains seleno culture medium, inoculation culture, the collection washing of cell, the processes such as fragmentation of cell: the said preparation that contains seleno culture medium is that Sodium Selenite is added in the wheat bran juice substratum, and the concentration that makes selenium is at 0.1～6g/L; Said inoculation culture is the aspergillus niger robe to be inoculated into contain in the seleno culture medium, cultivates 15～90 hours at 30～40 ℃; The collection washing of said cell is that the aspergillus niger cell that contains organoselenium is separated from contain seleno culture medium, uses with the isoosmotic physiological saline washing of aspergillus niger cell and removes the outer selenium of born of the same parents; Said cytoclasis, be to use high salt cell autolysis method or acid heat aqtocytolysis method lysing cell, nutritive substances such as organoselenium, amino acid, protein, ribose in the cell are discharged, make the organoselenium of aqueous solution form, and to make the content of organoselenium be 1～50mg/ml.
High salt cell autolysis method is to make the NaCl concentration in the self-dissolving system reach 1%～5%, temperature between 20～80 ℃, self-dissolving 5h～40h.Acid heat aqtocytolysis method be make the self-dissolving system the pH value in 2～7 scopes, temperature range is 20 ℃～70 ℃, self-dissolving 10min～30h regulates self-dissolving system pH to 6.3～7.3 with NaOH after self-dissolving is finished.
The preparation method of the finer selenium-rich vinegar of the present invention may further comprise the steps:
One, the preparation of organoselenium:
1, the substratum of preparation aspergillus niger growth.The aspergillus niger growth medium comprises the beans of bran mass, rich in proteins, comprise soya bean, black soya bean, mung bean etc., cereal with rich in starch, comprise barley, wheat, corn etc., and byproduct is the liquid nutrient medium that main raw material is made, also comprising the substratum that can support the aspergillus niger growth, also can be that the mixture of above two or more substratum is formed.In substratum, add the VITAMIN to support the aspergillus niger growth, vitamin H, biological micromolecule material, organic and inorganic salt, mineral substance, elemental metals etc., these materials include but not limited to vitamin H, VITMAIN B1, vitamin B6, calcium pantothenate, inositol, copper, copper sulfate, zinc, zinc sulfate, iron, and ferric sulfate.Also add an amount of microbiotic in the substratum and prevent microbiological contamination, microbiotic comprises other microbiotic such as tsiklomitsin, Streptomycin sulphate.The pH of substratum is controlled between 3～9, and preferred range is controlled between 4～7.
2, Sodium Selenite is added in the wheat bran juice substratum of preparation, the concentration that makes Sodium Selenite is between 10mg/L～500mg/L.
3, inoculation, cultivation aspergillus niger.Vaccination ways comprises the seed liquor inoculation, uses the spore direct inoculation, the song inoculation of making when aspergillus niger is produced, but be not limited to above several inoculation method.Culture temperature is at 30 ℃～40 ℃, preferably at 35 ℃～38 ℃.Incubation time is 15h～90h, and preferable range is 40h～70h.When using liquid nutrient medium in process of production, cultivate on vibrator or shaking table, rotating speed is at 50rpm～200rpm, and preferable range is at 120rpm～170rpm.Producing aspergillus niger selected in the rich selenium aspergillus niger can screen from environment, can be directly to buy from the type culture collection center, also can be to separate in song used from industrial production or the fermented liquid.
4, rich selenium aspergillus niger cell is collected in centrifugal or filtration, and the cell that washing is collected 2～10 times is removed and is adsorbed on the outer Sodium Selenite of cell walls.The aspergillus niger cell that will contain organoselenium is separated from contain selenium aspergillus niger substratum by centrifugal or filtration.Centrifugal rotation speed is 1000rpm～10000rpm, and preferred version is at 4000rpm～8000rpm.Filtration can be selected methods such as filter paper, gauze, absorbent cotton, filter funnel, suction filtration for use.
5, the applied chemistry method discharges the somatic cells fragmentation with nutritive ingredients such as the organoselenium in the born of the same parents, protein, nucleic acid, VITAMIN, polysaccharide, and making the aqueous solution volume of making that contains organoselenium is 1/100 of initial medium volume.Chemical process is meant that specifically the somatic cells self-dissolving adopts high salt cell autolysis method, perhaps acid heat aqtocytolysis method.Filter insoluble substances such as removing cell walls after the somatic cells self-dissolving, contain the organoselenium of 1～50mg/ml in the final aqueous solution organic selenium product.Salt in the high salt cell autolysis method is selected NaCl for use, adds the NaCl of solid form, and perhaps the NaCl with high concentration aqueous solution makes the NaCl concentration in the self-dissolving system reach 1%～5%, and preferred concentration is 2.5%～3.5%.The constant temperature self-dissolving, the temperature of self-dissolving can be between 20 ℃～80 ℃, and preferable range is between 50 ℃～60 ℃, and the self-dissolving time can be at 5h～40h, and the preferred time is 20h～30h.Acid heat aqtocytolysis method is to add HCl in the aspergillus niger cell of selenium outside the flush away born of the same parents, the pH value that makes the self-dissolving system is in 2～7 scopes, preferred pH value is 3.5～4.5, temperature range is 20 ℃～70 ℃, preferred temperature is 40 ℃～50 ℃, the self-dissolving time is 10min～30h, and the preferred self-dissolving time is 30min～2h.After finishing, self-dissolving regulates self-dissolving system pH to 6.3～7.3 with NaOH.
6, fluorescent method is measured the accurate content of selenium in the organoselenium solution, according to this volume of the organoselenium solution that adds in the vinegar that data computation needs to make toward per kilogram.The concrete steps of fluorescence spectrometry selenium content are carried out according to GB/T12399-1996.
Two, the fermentative production of common vinegar:
1, will be even, wetting with the raw material thorough mixing of other rich in starch such as the rice behind the water infiltration, wheat bran, rice husk, after soaking into 2 hours, steam material;
2, after raw material took the dish out of the pot, cooling immediately inserted kind of a bent and yeast/aspergillus niger, supplies moisture, fully turns evenly;
3, the song of having inoculated expects to carry out zymamsis into cylinder, will detect the temperature of vinegar unstrained spirits in the process of fermentation at any time, is controlled at suitable temperature, ferments 5-7 days, and after descending automatically to the unstrained spirits temperature, saccharification/zymamsis finishes;
4, open cylinder and add compound acetic acid bacteria strain, after fully mixing thoroughly, sealing cylinder mouth, change the acetic fermentation of vinegar unstrained spirits over to, monitoring temperature, through 6-7 days, acetic fermentation, measured the result in continuous two days, when acetic bacteria does not increase, residual alcohol is very little, below the unstrained spirits temperature drop to 38 ℃, when pickle sweet-smelling in the cylinder, little sweet, no astringent taste, acetic fermentation finishes substantially;
5, in time add salt and stir well, put the vinegar unstrained spirits of fermenting-ripening into the pouring pond and drench vinegar,
Three, in common vinegar, add organoselenium, make selenium-rich vinegar.
The organoselenium of making add after the fermentation of common vinegar or make conventional vinegar after add.The amount of the organoselenium that adds is strictly controlled, and the common vinegar of per kilogram adds 0.1～10mg organoselenium.
The vinegar that adds organoselenium is heated to 70 ℃-80 ℃, keeps sterilization in 30 minutes, clarifies 2-5 days, removes impurity after filtration, after the assay was approved packing.
The method for preparing selenium-rich vinegar of the present invention, because use the organoselenium that derives from aspergillus niger, therefore improved organoselenium transformation efficiency, shortened the cycle of producing, greatly reduced the cost of organoselenium, can control the organic selenium content in the vinegar exactly, help mending the production and the popularization of selenium vinegar.The organoselenium of prepared selenium-rich vinegar is by aspergillus niger Sodium Selenite to be converted into the selenium of organic form, and biosynthetic selenium derivative is because its toxicity is less and compare the easier Mammals institute metabolism that is with inorganic selenium salt, is selemium nutrition source preferably.The vinegar that derives from the aspergillus niger organoselenium is used by every canteen, for the crowd provides a kind of easy, micro-, lasting benefit selenium method, thereby can effectively prevent because the various diseases that causes of selenium deficiency, effectively improve the immunizing power of body, play preventing disease and function of health care.Therefore method of the present invention can be widely used in going in the productions such as food, seasonings, allows the vinegar that derives from the aspergillus niger organoselenium meet the need of market to greatest extent.
Embodiment
Concrete implementation step of the present invention is as follows, but and does not mean that limitation of the scope of the invention.
Embodiment 1
The 1kg soybean sprout adds water boil 30min, use filtered through gauze, add water to 10L, add 500g glucose again, 121 ℃ of sterilization 30min, add the 3.28g Sodium Selenite, make the liquid wheat bran juice substratum that contains 150mg/L selenium, picking aspergillus niger spore direct inoculation, 40 ℃, 200rpm, cultivate 20h, 8 layers of filtered through gauze are collected aspergillus niger cell, water washing 3 times, flush away is adsorbed on the outer Sodium Selenite of cell walls, add solid NaCl and reach 3%, 55 ℃ until concentration, self-dissolving 24h, self-dissolving is finished after-filtration and is removed insoluble composition, make the organoselenium of aqueous solution form, be settled to 100ml, the content that fluorescent method is measured organoselenium in the solution is 1.08mg/ml.
Will be even, wetting with the raw material thorough mixing of other rich in starch such as the rice behind the water infiltration, wheat bran, rice husk, after soaking into 2 hours, steam material.After raw material takes the dish out of the pot, be cooled to inoculation temp immediately, be about 30 ℃ summer, be below 40 ℃ winter, insert kind of a bent and yeast/aspergillus niger, supply moisture, fully turn evenly, go into the cylinder fermentation, to detect the temperature of vinegar unstrained spirits in the process of fermentation at any time, be controlled at suitable temperature, ferment 5～7 days, after the automatic decline of unstrained spirits temperature, saccharification/zymamsis finishes.Open cylinder and add compound acetic acid bacteria strain, after fully mixing thoroughly, sealing cylinder mouth, change the acetic fermentation of vinegar unstrained spirits over to, monitoring temperature, through 6～7 days, acetic fermentation, measured the result in continuous two days, when acetic bacteria does not increase, residual alcohol is very little, below the unstrained spirits temperature drop to 38 ℃, when pickle sweet-smelling in the cylinder, little sweet, no astringent taste, acetic fermentation finishes substantially.In time add salt and stir well, put the vinegar unstrained spirits of fermenting-ripening into the pouring pond and drench vinegar.
Per kilogram drenches the organoselenium aqueous solution that adds the 5ml preparation in the vinegar that, and the content that makes organoselenium in the vinegar is 5.4mg/Kg.The vinegar that will contain organoselenium is heated to 70 ℃-80 ℃, keeps 30 minutes, after the sterilization, clarifies 2～5 days, removes by filter impurity, packing after the assay was approved again.
Embodiment 2
Raw material and the organoselenium preparation process that derives from aspergillus niger are with embodiment 1, just acid heat aqtocytolysis method is adopted in the somatic cells fragmentation, promptly add HCl to pH and be worth 4.5,45 ℃, self-dissolving 1.5h, 4000rpm regulates self-dissolving system pH to 7 with NaOH after self-dissolving is finished, remove by filter insoluble composition, make the organoselenium of aqueous solution form.
The vinegar preparation is with embodiment 1.Afterwards, per kilogram drenches the organoselenium aqueous solution that adds the 0.46ml preparation in the vinegar that, and the content that makes organoselenium in the vinegar is 0.5mg/Kg.The aftertreatment that contains the selenium vinegar is also with embodiment 1.
Embodiment 3
Raw material and the organoselenium preparation process that derives from aspergillus niger are with embodiment 1, just in wheat bran juice substratum, add the 131.2g Sodium Selenite, make the wheat bran juice substratum of 6g/L selenium, inoculate back 30 ℃ and cultivate 90h, the content that the organoselenium solution that finally makes is measured organoselenium with fluorescent method is 39.65mg/ml.
The vinegar preparation is with embodiment 1.Afterwards, per kilogram drenches the organoselenium aqueous solution that adds the 0.25ml preparation in the vinegar that, and the content that makes organoselenium in the vinegar is 9.82mg/Kg.The aftertreatment that contains the selenium vinegar is also with embodiment 1.
Claims (3)
1, a kind of vinegar that derives from the aspergillus niger organoselenium that contains is to add organoselenium in vinegar, it is characterized in that organoselenium is the organoselenium that derives from aspergillus niger, and the content of organoselenium is 0.1～10mg/kg in the vinegar.
2, a kind of preparation method who contains the vinegar that derives from the aspergillus niger organoselenium, the said vinegar that derives from the aspergillus niger organoselenium that contains is to add organoselenium in vinegar, the content of organoselenium is 0.1～10mg/kg in the vinegar; Its preparation method is divided into the preparation of organoselenium, the preparation and the organoselenium of vinegar adds three steps in the vinegar to; With rice, wheat bran, rice husk is starting raw material, makes conventional vinegar through pulverizing, boiling, koji, solid state fermentation; In the vinegar of making, add organoselenium; It is characterized in that,
The organoselenium that adds in the vinegar transforms preparation by aspergillus niger;
The preparation of organoselenium comprises the preparation that contains seleno culture medium, inoculation culture, the collection washing of cell, the processes such as fragmentation of cell: the said preparation that contains seleno culture medium, be that Sodium Selenite is added in the wheat bran juice substratum, the concentration that makes Sodium Selenite is at 0.1～6g/L; Said inoculation culture is the aspergillus niger robe to be inoculated into contain in the seleno culture medium, cultivates 15～90 hours at 30～40 ℃; The collection washing of said cell is that the aspergillus niger cell that contains organoselenium is separated from contain seleno culture medium, uses with the isoosmotic physiological saline washing of aspergillus niger cell and removes the outer selenium of born of the same parents; Said cytoclasis, be to use high salt cell autolysis method or acid heat aqtocytolysis method lysing cell, nutritive substances such as organoselenium, amino acid, protein, ribose in the cell are discharged, make the organoselenium of aqueous solution form, and to make the content of organoselenium be 1～50mg/ml.
3, according to the described preparation method who contains the vinegar that derives from the aspergillus niger organoselenium of claim 2, it is characterized in that, in the said cytoclasis process, using high salt cell autolysis method lysing cell is to make the NaCl concentration in the self-dissolving system reach 1%～5%, temperature between 20～80 ℃, self-dissolving 5h～40h; Use acid heat aqtocytolysis method lysing cell and be make the self-dissolving system pH value in 2～7 scopes, temperature range is 20 ℃～70 ℃, self-dissolving 10min～30h, after self-dissolving is finished with NaOH adjusting self-dissolving system pH to 6.3～7.3.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410011259 CN1632099B (en) | 2004-11-25 | 2004-11-25 | Vinegar containing organic selenium from aspergillus niger and method for preparing same |
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| CN 200410011259 CN1632099B (en) | 2004-11-25 | 2004-11-25 | Vinegar containing organic selenium from aspergillus niger and method for preparing same |
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| CN1632099A true CN1632099A (en) | 2005-06-29 |
| CN1632099B CN1632099B (en) | 2010-09-08 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102839132A (en) * | 2012-09-21 | 2012-12-26 | 中粮生物化学（安徽）股份有限公司 | Aspergillus niger moldy bran culture medium and preparation method thereof, and Aspergillus niger moldy bran culture method |
| CN104109622A (en) * | 2014-01-22 | 2014-10-22 | 湖北工业大学 | Preparation method of selenium-rich edible vinegar |
| CN112553037A (en) * | 2020-12-24 | 2021-03-26 | 江苏大学 | Preparation method of vinegar rich in active selenium |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1062597C (en) * | 1996-06-28 | 2001-02-28 | 牛西午 | Selenium-strontium-rich black-buckwheat health-care vinegar and preparation method thereof |
| CN1148089A (en) * | 1996-09-09 | 1997-04-23 | 孙学 | Selenium-rich vinegar and method for prepn. of same |
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2004
- 2004-11-25 CN CN 200410011259 patent/CN1632099B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102839132A (en) * | 2012-09-21 | 2012-12-26 | 中粮生物化学（安徽）股份有限公司 | Aspergillus niger moldy bran culture medium and preparation method thereof, and Aspergillus niger moldy bran culture method |
| CN104109622A (en) * | 2014-01-22 | 2014-10-22 | 湖北工业大学 | Preparation method of selenium-rich edible vinegar |
| CN104109622B (en) * | 2014-01-22 | 2017-01-18 | 湖北工业大学 | Preparation method of selenium-rich edible vinegar |
| CN112553037A (en) * | 2020-12-24 | 2021-03-26 | 江苏大学 | Preparation method of vinegar rich in active selenium |
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| CN1632099B (en) | 2010-09-08 |
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