CN1625951A - Microbe fungus agent of raising copper absorption for plant and preparation method - Google Patents
Microbe fungus agent of raising copper absorption for plant and preparation method Download PDFInfo
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- CN1625951A CN1625951A CN 200310106561 CN200310106561A CN1625951A CN 1625951 A CN1625951 A CN 1625951A CN 200310106561 CN200310106561 CN 200310106561 CN 200310106561 A CN200310106561 A CN 200310106561A CN 1625951 A CN1625951 A CN 1625951A
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- mycorrhizal fungi
- arbuscular mycorrhizal
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Abstract
A microbial prararation for increasing the absorptivity of plant to copper contains more useful microbes including scotch blastocyst, panicillium sp., etc. It is prepared by culturing them in liquid-state Ca's culture medium.
Description
Technical field
The present invention relates to a kind of high-effective microorganism microbial inoculum and preparation method thereof, especially a kind of plant copper that can improve absorbs microbial bacterial agent and preparation method thereof.
Background technology
At present, mostly public microbial manure is to be made up of bacteriums such as azotobacter, phosphobacteria, potassium bacterium, photosynthetic bacteria, bacillus, silicate bacteriums, improves the yield and quality of plant by the nutrition condition of improving plant.For chemical fertilizer, the application of these products has play a part positive for the protection of ecotope, but these microbial manures are often powerless for the biological restoration of soil pollution especially heavy metal pollution.
And mostly the arbuscular mycorrhiza microbial inoculum that occurs in recent years is to concentrate on the mineral nutrition that improves plant, the several aspects such as growth that improve drought resistance, promotion plant also, disclose a kind of arbuscular mycorrhiza microbial inoculum and production method thereof in the specification as No. 00137712.4, Chinese patent, but yet there are no patent or the product that improves plant heavy metal resistance aspect with drought resisting phosphorus decomposing function.
Summary of the invention
The object of the present invention is to provide a kind of microbial bacterial agent that can improve the absorption of plant copper and preparation method thereof, this microbial bacterial agent can improve the resistance that plant pollutes copper effectively, promotes the growth of plant, improves the absorptive amount to copper.
Above-mentioned purpose of the present invention is achieved in that a kind of microbial bacterial agent that can improve the absorption of plant copper, it is the composite microorganism viable bacteria agent that contains the multiple beneficial microorganism, comprise Scotland sacculus mould (Glomus caledonium), the huge sporangiocyst of pearl mould (Gigaspora margarita), easy mistake huge sporangiocyst mould (Gigaspora decipens), David Gilmour Scutellospora (Scutellospora gilmori), it is characterized in that: the Penicillium notatum that also comprises multiple arbuscular mycorrhizal fungi and anti-copper, described arbuscular mycorrhizal fungi is the arbuscular mycorrhizal fungi of acaulospora specie (Acaulospora) and Paraglomus (Glomus), and the Penicillium notatum of described anti-copper is pale purple mould (Penicillium lilacinum) and point-like mould (Penicillium notatum).
Microbial bacterial agent of the present invention, it is characterized in that: also comprise and contain several mineral materials and organic medium, described medium is matrix river sand, peat and the plant root segment of cultivation arbuscular mycorrhizal fungi, and sodium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, potassium chloride, ferrous sulfate and the sucrose of cultivating Penicillium notatum.
The present invention also provides a kind of preparation method who improves the microbial bacterial agent of plant copper absorption, it is characterized in that having following process:
A, comprise the expansion production process of an arbuscular mycorrhizal fungi microbial inoculum at least, the expansion production process of this arbuscular mycorrhizal fungi microbial inoculum has following steps:
1. prepare inoculum: with the soil sample that contains arbuscular mycorrhizal fungi spore, mycelia and infected the host plant root segment as inoculum;
2. prepare matrix: as matrix, and sneak into urea and superphosphate with river sand and peat;
3. plant host plant: in matrix, excute a law with layer or the mixed above-mentioned inoculum of access of excuting a law, in matrix, add entry then by the inoculum concentration of mass ratio 3%-5%, the seed of sowing host plant, Routine Management made the host plant growth 3-4 month;
4. the overground part of host plant is cut off, get the soil of host plant root segment and host plant root segment, shred host plant root segment mixing, promptly get the arbuscular mycorrhizal fungi microbial inoculum after air-dry;
B, comprise the expansion production process of a Penicillium notatum at least, it is that the brood body of Penicillium notatum is inserted in the liquid Czapek's medium, and concussion was cultivated 3 days, must contain the liquid nutrient medium of Penicillium notatum;
C, the liquid nutrient medium that contains Penicillium notatum that is obtained by process B is admixed mixing in the arbuscular mycorrhizal fungi that is obtained by process A, promptly get after air-dry and improve the microbial bacterial agent that plant copper absorbs.
Method of the present invention is characterized in that, described host plant is clover, Sudan grass or corn.
Method of the present invention is characterized in that, described matrix is earlier river sand and peat to be crossed the 1mm sieve, mixes in 1: 1 by volume then to make, and the amount of sneaking into urea is 0.078 gram/kilogram matrix, and the amount of sneaking into superphosphate is 0.33 gram/kilogram matrix.
Method of the present invention is characterized in that, the spore density of arbuscular mycorrhizal fungi is greater than 10/gram matrix in the described arbuscular mycorrhizal fungi microbial inoculum.
Method of the present invention is characterized in that, the temperature that described concussion is cultivated is 30 ℃.
Method of the present invention is characterized in that, the spore count of described Penicillium notatum is greater than 10
9Individual/milliliter.
Method of the present invention is characterized in that, the liquid nutrient medium that contains Penicillium notatum is admixed mixing in the arbuscular mycorrhizal fungi microbial inoculum according to the ratio of 100 ml/kg.
Below, in conjunction with specific embodiments, the present invention is described in further detail.
Embodiment
River sand and peat are crossed the 1mm sieve, according to 1: 1 volume mixture, and sneak into certain amount of urea (0.078 gram/kilogram matrix) and superphosphate (0.33 gram/kilogram matrix), in the plastic box of packing into after weighing.As inoculum, inoculum concentration is 3%-5% with the soil sample of multiple arbuscular mycorrhizal fungi that contains Scotland sacculus mould (Glomus caledonium), the huge sporangiocyst of pearl mould (Gigasporamargarita), easily miss huge sporangiocyst mould (Gigaspora decipens), David Gilmour Scutellospora (Scutellospora gilmori) and acaulospora specie (Acaulospora) and Paraglomus (Glomus).Mixing excutes a law admixes in the matrix, pours into the water about 20%, the sowing Sudan grass.Routine Management, the spore density of checking arbuscular mycorrhiza in the soil after 3 months whether＞10/gram dry ground, reach standard after, the Sudan grass acrial part is cut off, root is shredded, with the matrix mixing, air-dry stand-by.
Insert the triangular flask that liquid Czapek's medium is housed (in 250～500ml), 30 ℃ of concussions were cultivated 3 days being kept at Penicillium notatum brood body on the fungi culture medium inclined-plane.Check the spore count (＞10 in the medium
9Individual/milliliter).If meet the requirements, the liquid nutrient medium that contains Penicillium notatum is admixed mixing in the air-dry arbuscular mycorrhizal fungi microbial inoculum according to the ratio of 100 ml/kg, application can be preserved in air-dry back under normal temperature condition.
With being seeded in the copper contaminated soil after this microbial inoculum and the plant seed seed dressing, to compare with the plant that does not mix microbial inoculum, the upgrowth situation of plant obviously improves, and biomass and copper content significantly increase, thereby have improved the phytoremediation efficient that copper pollutes.
Claims (6)
1, improves the microbial bacterial agent that plant copper absorbs, it is the composite microorganism viable bacteria agent that contains the multiple beneficial microorganism, comprise that Scotland sacculus is mould, the huge sporangiocyst of pearl is mould, miss easily that huge sporangiocyst is mould, the David Gilmour Scutellospora, it is characterized in that: the Penicillium notatum that also comprises multiple arbuscular mycorrhizal fungi and anti-copper, described arbuscular mycorrhizal fungi is the arbuscular mycorrhizal fungi of acaulospora specie and Paraglomus, and the Penicillium notatum of described anti-copper is pale purple mould and point-like mould.
2, microbial bacterial agent as claimed in claim 1, it is characterized in that: also comprise and contain several mineral materials and organic medium, described medium is matrix river sand, peat and the plant root segment of cultivation arbuscular mycorrhizal fungi, and sodium nitrate, dipotassium hydrogen phosphate, magnesium sulfate, potassium chloride, ferrous sulfate and the sucrose of cultivating Penicillium notatum.
3, a kind of preparation method who improves the microbial bacterial agent of plant copper absorption is characterized in that having following process:
A, comprise the expansion production process of an arbuscular mycorrhizal fungi microbial inoculum at least, the expansion production process of this arbuscular mycorrhizal fungi microbial inoculum has following steps:
1. prepare inoculum: with the soil sample that contains arbuscular mycorrhizal fungi spore, mycelia and infected the host plant root segment as inoculum;
2. prepare matrix: as matrix, and sneak into urea and superphosphate with river sand and peat;
3. plant host plant: in matrix, excute a law with layer or the mixed above-mentioned inoculum of access of excuting a law, in matrix, add entry then by the inoculum concentration of mass ratio 3%-5%, the seed of sowing host plant, Routine Management made the host plant growth 3-4 month;
4. the overground part of host plant is cut off, get the soil of host plant root segment and host plant root segment, shred host plant root segment mixing, promptly get the arbuscular mycorrhizal fungi microbial inoculum after air-dry;
B, comprise the expansion production process of a Penicillium notatum at least, it is that the brood body of Penicillium notatum is inserted in the liquid Czapek's medium, and 30 ℃ of concussions were cultivated 3 days, must contain the liquid nutrient medium of Penicillium notatum;
C, the liquid nutrient medium that contains Penicillium notatum is admixed mixing in the arbuscular mycorrhizal fungi microbial inoculum, promptly get after air-dry and improve the microbial bacterial agent that plant copper absorbs.
4, method as claimed in claim 3 is characterized in that, described host plant is clover, Sudan grass or corn.
5, method as claimed in claim 3, it is characterized in that described matrix is that the 1mm sieve is crossed with river sand and peat by elder generation, mixes in 1: 1 by volume then to make, the amount of sneaking into urea is 0.078 gram/kilogram matrix, and the amount of sneaking into superphosphate is 0.33 gram/kilogram matrix.
6, method as claimed in claim 3 is characterized in that, the liquid nutrient medium that contains Penicillium notatum is admixed mixing in the arbuscular mycorrhizal fungi microbial inoculum according to the ratio of 100 ml/kg.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200310106561 CN1625951A (en) | 2003-12-09 | 2003-12-09 | Microbe fungus agent of raising copper absorption for plant and preparation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200310106561 CN1625951A (en) | 2003-12-09 | 2003-12-09 | Microbe fungus agent of raising copper absorption for plant and preparation method |
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| CN1625951A true CN1625951A (en) | 2005-06-15 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104707864A (en) * | 2015-03-04 | 2015-06-17 | 蒋常德 | Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
| CN107227509A (en) * | 2017-06-27 | 2017-10-03 | 江苏大学 | A kind of nano material microfibre using fungal hyphae as template and preparation method thereof |
| CN107821023A (en) * | 2017-11-20 | 2018-03-23 | 内蒙古医科大学 | The method that Actions of Clematis Species general flavone content and yield are improved using AMF |
| CN109731909A (en) * | 2019-02-13 | 2019-05-10 | 辽宁大学 | The method of reed-AMF symbiosis consolidation system reparation Cu-W ore deposit |
| CN112028713A (en) * | 2020-09-15 | 2020-12-04 | 湖南亿康环保科技有限公司 | Passivator for treating soil arsenic pollution and treatment method |
| CN112889564A (en) * | 2021-01-12 | 2021-06-04 | 南京农业大学 | Native AMF mixed microbial inoculum of fruit trees as well as propagation method and application thereof |
-
2003
- 2003-12-09 CN CN 200310106561 patent/CN1625951A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104707864A (en) * | 2015-03-04 | 2015-06-17 | 蒋常德 | Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof |
| CN107227509A (en) * | 2017-06-27 | 2017-10-03 | 江苏大学 | A kind of nano material microfibre using fungal hyphae as template and preparation method thereof |
| CN107227509B (en) * | 2017-06-27 | 2020-06-09 | 江苏大学 | Nano material microfiber using mold hyphae as template and preparation method thereof |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
| CN107821023A (en) * | 2017-11-20 | 2018-03-23 | 内蒙古医科大学 | The method that Actions of Clematis Species general flavone content and yield are improved using AMF |
| CN109731909A (en) * | 2019-02-13 | 2019-05-10 | 辽宁大学 | The method of reed-AMF symbiosis consolidation system reparation Cu-W ore deposit |
| CN112028713A (en) * | 2020-09-15 | 2020-12-04 | 湖南亿康环保科技有限公司 | Passivator for treating soil arsenic pollution and treatment method |
| CN112889564A (en) * | 2021-01-12 | 2021-06-04 | 南京农业大学 | Native AMF mixed microbial inoculum of fruit trees as well as propagation method and application thereof |
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