CN1603401A - Ganoderma lucidum mycelium with tea as matrix by double fermentation and its production method - Google Patents
Ganoderma lucidum mycelium with tea as matrix by double fermentation and its production method Download PDFInfo
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Abstract
Take tea as matrix double fermentation production ganoderma lucidum mycelium and its preparation method, ganoderma lucidum mycelium's Mars effective component for tea polyphenol 100~~250g/Kg, ganoderma lucidum polysaccharide 20~~30g/Kg, protein 200~~400g/Kg, vitamin 40~~100g/Kg. Its preparation method is, uses double lives the fermentation, the single layer vaccinates the yeast in the tea foundation, ferments through the solid state, obtains including the tea polyphenol, the inorganic mineral substance element, the vitamin, the yeast egg waits in vain the many kinds of active nutrition metabolism product, the tea area south of Yellow River has has the side effect to the human body Coffee Alkalito obtain the decomposition, the content reduces. Double in in above metabolism product foundation, after deactivation vaccinates the ganoderma lucidum fungus, the second solid state ferments, obtains including the tea polyphenol, massive organic mineral substance element, many kinds of vitamins, amino acid, protein, ganoderma lucidum polysaccharide and so on many kinds of effective active constituent ganoderma lucidum mycelium. He retained the tea unique flavor and the feeling in the mouth, after causes the processing the broken tea leaves to recycle waste.
Description
Technical field:
The present invention relates to field of deep tea processing technology, more specifically to tealeaves being Ganoderma mycelium of matrix production and preparation method thereof.
Background technology:
Traditional tealeaves deep processing, general employing goes out the molten water logging of tealeaves, adds chemical agent heat again and carries, and makes the precipitations such as effective constituent tea-polyphenol in the tealeaves, and filtering then purifies obtains.The deficiency of this method is: the effective constituent in the tealeaves is except that catechin, Flavonoid substances part are suggested with tea-polyphenol, and other effective constituent such as inorganic mineral element, VITAMIN etc. fail to obtain to extract.
Traditional Ganoderma mycelium is a matrix with wood chip, wheat bran, sugarcane slag or soybean protein, and the inoculation Ganderma lucidum by liquid state fermentation, filters purification and obtains.
Have at present a kind of with tealeaves as matrix, add wheat bran, Semen Maydis powder etc., the inoculation Ganderma lucidum obtains the production technique of " fermented glossy ganoderma tea " by solid state fermentation, the effective constituent in this technology tealeaves has obtained a certain proportion of conversion, but complete inadequately.Owing to added wheat bran, Semen Maydis powder, influenced the taste flavor of fermented tea to a great extent in addition.
Summary of the invention:
Technical problem to be solved by this invention is to overcome the existing deficiency that the fermented glossy ganoderma tea mouthfeel is not good, its production technique fails to make the effective constituent in the tealeaves fully to be transformed, and is that the safety non-toxic of matrix two-fold fermentative production is convenient to Ganoderma mycelium that absorption of human body utilizes and preparation method thereof with tealeaves and provide a kind of.
The present invention is achieved through the following technical solutions:
With tealeaves is matrix two-fold fermented mycelia, and the active effective constituent of this Ganoderma mycelium is as follows by weight,
Tea-polyphenol 100～250g/Kg
Ganoderan 20～30g/Kg
Protein 20～400g/Kg
VITAMIN 40～100g/Kg
Organic mineral element 30～350g/Kg
Wherein, organic mineral is elementary composition for by weight
Potassium 14～30g/Kg
Silicon 14～30g/Kg
Magnesium 150～500mg/Kg
Manganese 380～800mg/Kg
Fluorine 150～500mg/Kg
Calcium 300～400mg/Kg
Sodium 200～800mg/Kg
Sulphur 500～800mg/Kg
Iron 60～100mg/Kg
Copper 50～60mg/Kg
Zinc 40～70mg/Kg
Nickel 5～28mg/Kg
Selenium 100～250mg/Kg
Germanium 100～350mg/Kg
VITAMIN consists of by weight,
Vitamins B 80～130mg/Kg
Vitamins C 500～3000mg/Kg
Vitamin-E 200～800mg/Kg
Vitamin U 150～250mg/Kg
Vitamin K 3000～5000mg/Kg.
With tealeaves is the preparation method of matrix two-fold fermented mycelia,
A, the first heavy solid state fermentation i.e. inoculation yeast bacterium in tealeaves matrix, it with tealeaves matrix according to tealeaves by weight: vacuum-packed active 90% yeast: sucrose: lime carbonate: potassium primary phosphate: water=90～96: 1～5: 1～2: 1～1.5: 1～1.5: 60～65 ratio, in tealeaves matrix, add yeast, sucrose, lime carbonate, potassium primary phosphate, water and transfer pH value to 6.5～7.0, fully mix relief tealeaves matrix solid state fermentation 9～12 hours under saccharomycetic effect;
B, deactivation will be through the product behind the first heavy solid state fermentation with the high pressure steam sterilizations of 120 ℃～125 ℃ of pressure 0.13～0.15Mpa, temperature 1.3～2 hours;
C, the second heavy solid state fermentation are promptly inoculated Ganderma lucidum in the product after deactivation, wherein tealeaves matrix provides the Ganoderma lucidum mycelium bulk-growth required carbon source, and yeast provides the Ganoderma lucidum mycelium bulk-growth required nitrogenous source.When treating that product after deactivation is cooled to 20～25 ℃, in this product,, fermented 27～33 days according to the every 100Ml inoculation of volume ratio 1～5Ml liquid Ganderma lucidum strain;
D, detection are carried out ganoderan to the meta-bolites behind the second heavy solid state fermentation and are detected, and require ganoderma polyoses content greater than carrying out next process at 2% o'clock;
E, vacuum-drying are carried out vacuum-drying to the meta-bolites after greater than 2% the second heavy solid state fermentation of back ganoderma polyoses content after testing, and drying conditions is 50～70 ℃ of vacuum tightness 0.9～0.96Mpa, temperature; After making its water content be lower than 9%, carry out next process again;
F, to be broken to through the product after the vacuum-drying particle diameter less than 60 orders below, again with carrying out irradiation sterilization after the paper pack, irradiation dose is less than 10KGY, when bacteria content less than 10
4Get product behind the cfu/g.
Described tealeaves is chosen the fannings after ordinary tea leaves or the Tea Processing.
The Na that adds 100～250ppm in the described tealeaves matrix
2SeO
3Or the GeO of 100～350ppm
2Or the ZnSO of 100～450ppm
4Can be made into the tealeaves Ganoderma mycelium of rich selenium or rich germanium or rich zinc.
Outstanding effect of the present invention is:
1), patent of the present invention is matrix with tealeaves, adopt double hair tonic ferment, first focuses on inoculation yeast bacterium on the tealeaves basis, pass through solid state fermentation, acquisition comprises the meta-bolites of various active nutritive ingredients such as tea-polyphenol (containing catechin, Flavonoid substances), inorganic mineral element (part begins to organise through fermentation), VITAMIN, Yeast protein, original in the tealeaves have the trimethyl-xanthine of side effect to obtain decomposing to human body, and content reduces.Second is heavy then on above-mentioned meta-bolites basis, inoculate Ganderma lucidum after the deactivation, solid state fermentation for the second time obtains comprising the Ganoderma mycelium of multiple effective active compositions such as tea-polyphenol, a large amount of organic mineral element, multivitamin, amino acid, protein, ganoderan.
2), the suitable all kinds of tealeaves of the raw materials for production of patent of the present invention, especially the fannings after the Tea Processing, wide material sources, cheap, such one of China is produced tea big country,, rationally utilize the tealeaves bioactive ingredients improving tealeaves deep processing ratio, fannings after the processing is turned waste into wealth, significant.
3), the present invention has improved the conversion and the utilization of tealeaves active nutrient component greatly, do not add other ancillary components, distinctive local flavor of tealeaves and mouthfeel have been kept, both can make confession people such as teabag, electuary particle, tea drink drinks, also can be processed into capsule and take, make those crowds that are not suitable for drinking tea obtain the chance of additional effective content of tea for the people.
4), in tealeaves matrix, add inorganic selenium (germanium, zinc), utilize enrichment and the organic transformation effect of yeast and Ganderma lucidum to micro-rare elements selenium (germanium, zinc), make and be rich in organoselenium (germanium, zinc) in the Ganoderma mycelium, thereby utilize organoselenium (germanium, zinc) the distinctive health-care effect of human body to be made the tealeaves matrix Ganoderma mycelium that is rich in organoselenium (germanium, zinc).
Description of drawings:
Accompanying drawing 1 is a process flow sheet of the present invention.
Embodiment:
See accompanying drawing 1,
With tealeaves is the preparation method of matrix two-fold fermented mycelia,
A, the first heavy solid state fermentation i.e. inoculation yeast bacterium in tealeaves matrix, with the fannings after the Tea Processing is matrix according to tealeaves by weight: vacuum-packed active 90% yeast: sucrose: lime carbonate: potassium primary phosphate: water=90～96: 1～5: 1～2: 1～1.5: 1～1.5: 60～65 ratio, in tealeaves matrix, add yeast, sucrose, lime carbonate, potassium primary phosphate, water and transfer pH value to 6.5～7.0, fully mix relief tealeaves matrix solid state fermentation 9～12 hours under saccharomycetic effect; Also can in the tea mesostroma, add a spot of inorganic selenium (germanium, zinc) element.
B, deactivation will be through the product behind the first heavy solid state fermentation with the high pressure steam sterilizations of 120 ℃～150 ℃ of 0.13～0.15Mpa, temperature 1.3～2 hours;
C, the second heavy solid state fermentation are promptly inoculated Ganderma lucidum in the product after deactivation, when treating that product after deactivation is cooled to 20～25 ℃, according to the every 100Ml inoculation of volume ratio 1～5Ml liquid Ganderma lucidum strain, fermented 27～33 days in this product;
D, detection are carried out ganoderan to the meta-bolites behind the second heavy solid state fermentation and are detected, and require ganoderma polyoses content greater than carrying out next process at 2% o'clock;
E, vacuum-drying are carried out vacuum-drying to the meta-bolites after greater than 2% the second heavy solid state fermentation of back ganoderma polyoses content after testing, and drying conditions is 50～70 ℃ of vacuum tightness 0.9～0.96Mpa, temperature; After making its water content be lower than 9%, carry out next process again;
F, to be broken to through the product after the vacuum-drying particle diameter less than 60 orders below, again with carrying out irradiation sterilization after the paper pack, irradiation dose is less than 10KGY, when bacteria content less than 10
4Get product behind the cfu/g.
By the Ganoderma mycelium that above method production makes, active effective constituent is as follows by weight,
Tea-polyphenol 100～250g/Kg
Ganoderan 20～30g/Kg
Protein 200～400g/Kg
VITAMIN 40～100g/Kg
Organic mineral element 30～350g/Kg
Wherein, organic mineral is elementary composition for by weight
Potassium 14～30g/Kg
Silicon 14～30g/Kg
Magnesium 150～500mg/Kg
Manganese 380～800mg/Kg
Fluorine 150～500mg/Kg
Calcium 300～400mg/Kg
Sodium 200～800mg/Kg
Sulphur 500～800mg/Kg
Iron 60～100mg/Kg
Copper 50～60mg/Kg
Zinc 40～70mg/Kg
Nickel 5～28mg/Kg
Selenium 100～250mg/Kg
Germanium 100～350mg/Kg
VITAMIN consists of by weight,
Vitamins B 80～130mg/Kg
Vitamins C 500～3000mg/Kg
Vitamin-E 200～800mg/Kg
Vitamin U 150～250mg/Kg
Vitamin K 3000～5000mg/Kg.
Claims (4)
1, be matrix two-fold fermented mycelia with tealeaves, it is characterized in that: the active effective constituent of this Ganoderma mycelium is as follows by weight,
Tea-polyphenol 100～250g/Kg
Ganoderan 20～30g/Kg
Protein 200～400g/Kg
VITAMIN 40～100g/Kg
Organic mineral element 30～350g/Kg
Wherein, organic mineral is elementary composition for by weight
Potassium 14～30g/Kg
Silicon 14～30g/Kg
Magnesium 150～500mg/Kg
Manganese 380～800mg/Kg
Fluorine 150～500mg/Kg
Calcium 300～400mg/Kg
Sodium 200～800mg/Kg
Sulphur 500～800mg/Kg
Iron 60～100mg/Kg
Copper 50～60mg/Kg
Zinc 40～70mg/Kg
Nickel 5～28mg/Kg
Selenium 100～250mg/Kg
Germanium 100～350mg/Kg
VITAMIN consists of by weight,
Vitamins B 80～130mg/Kg
Vitamins C 500～3000mg/Kg
Vitamin-E 200～800mg/Kg
Vitamin U 150～250mg/Kg
Vitamin K 3000～5000mg/Kg.
2, be the preparation method of matrix two-fold fermented mycelia with tealeaves, it is characterized in that:
A, the first heavy solid state fermentation i.e. inoculation yeast bacterium in tealeaves matrix, it with tealeaves matrix according to tealeaves by weight: vacuum-packed active 90% yeast: sucrose: lime carbonate: potassium primary phosphate: water=90～96: 1～5: 1～2: 1～1.5: 1～1.5: 60～65 ratio, in tealeaves matrix, add yeast, sucrose, lime carbonate, potassium primary phosphate, water and transfer pH value to 6.5～7.0, fully mix relief tealeaves matrix solid state fermentation 9～12 hours under saccharomycetic effect;
B, deactivation will be through the product behind the first heavy solid state fermentation with the high pressure steam sterilizations of 120 ℃～125 ℃ of 0.13～0.15Mpa, temperature 1.3～2 hours;
C, the second heavy solid state fermentation are promptly inoculated Ganderma lucidum in the product after deactivation, when treating that product after deactivation is cooled to 20～25 ℃, according to the every 100Ml inoculation of volume ratio 1～5Ml liquid Ganderma lucidum strain, fermented 27～33 days in this product;
D, detection are carried out ganoderan to the meta-bolites behind the second heavy solid state fermentation and are detected, and require ganoderma polyoses content greater than carrying out next process at 2% o'clock;
E, vacuum-drying are carried out vacuum-drying to the meta-bolites after greater than 2% the second heavy solid state fermentation of back ganoderma polyoses content after testing, and drying conditions is 50～70 ℃ of vacuum tightness 0.9～0.96Mpa, temperature; After making its water content be lower than 9%, carry out next process again;
F, to be broken to through the product after the vacuum-drying particle diameter less than 60 orders below, again with carrying out irradiation sterilization after the paper pack, irradiation dose is less than 10KGY, when bacteria content less than 10
4Get product behind the cfu/g.
3, described according to right 1 is the preparation method of matrix two-fold fermented mycelia with tealeaves, and it is characterized in that: tealeaves is chosen the fannings after ordinary tea leaves or the Tea Processing.
4, described according to right 1 is the preparation method of organic selenium-rich (germanium, the zinc) Ganoderma mycelium of matrix two-fold fermentative production with tealeaves, it is characterized in that: the Na that adds 100～250ppm in the tealeaves matrix
2SeO
3Or the GeO of 100～350ppm
2Or the ZnSO of 100～450ppm
4Can be made into the tealeaves Ganoderma mycelium of rich selenium or rich germanium or rich zinc.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101492174B1 (en) | 2013-01-24 | 2015-02-10 | 주식회사 코시스바이오 | Anti-inflammatory and immune-boosting composition containing fermented green coffee bean which is fermented with monascus |
| CN105009930A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Production method for selenium-enriched ganoderma mycelium |
| CN107593955A (en) * | 2017-11-03 | 2018-01-19 | 于葵 | Ferment edible mushroom tea preparation process and fermentation edible mushroom tea |
| CN107647009A (en) * | 2017-08-14 | 2018-02-02 | 湖南味菇坊生物科技股份有限公司 | Wave fine and soft ganoderma tea and preparation method |
| CN110537597A (en) * | 2019-09-29 | 2019-12-06 | 横县南方茶厂 | processing method of ganoderma lucidum green tea |
| CN114250153A (en) * | 2021-12-15 | 2022-03-29 | 江南大学 | High-selenium-resistance Ganoderma lucidum JNUSE-200 and selenium-rich fermentation strategy thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1076591C (en) * | 1998-02-27 | 2001-12-26 | 福建省农业科学院土壤肥料研究所 | Technology for producing fermented glossy ganoderma tea |
-
2004
- 2004-10-13 CN CNB2004100518178A patent/CN100455652C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101492174B1 (en) | 2013-01-24 | 2015-02-10 | 주식회사 코시스바이오 | Anti-inflammatory and immune-boosting composition containing fermented green coffee bean which is fermented with monascus |
| CN105009930A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Production method for selenium-enriched ganoderma mycelium |
| CN105009930B (en) * | 2014-04-25 | 2018-03-09 | 江苏中祥高科技实业有限公司 | A kind of Se-rich lucid ganoderma mycelium production method |
| CN107647009A (en) * | 2017-08-14 | 2018-02-02 | 湖南味菇坊生物科技股份有限公司 | Wave fine and soft ganoderma tea and preparation method |
| CN107593955A (en) * | 2017-11-03 | 2018-01-19 | 于葵 | Ferment edible mushroom tea preparation process and fermentation edible mushroom tea |
| CN110537597A (en) * | 2019-09-29 | 2019-12-06 | 横县南方茶厂 | processing method of ganoderma lucidum green tea |
| CN114250153A (en) * | 2021-12-15 | 2022-03-29 | 江南大学 | High-selenium-resistance Ganoderma lucidum JNUSE-200 and selenium-rich fermentation strategy thereof |
| CN114250153B (en) * | 2021-12-15 | 2023-07-25 | 江南大学 | High selenium-resistant ganoderma Ganoderma lucidum JNUSE-200 and selenium-rich fermentation strategy thereof |
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