CN1600855A - Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase - Google Patents
Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase Download PDFInfo
- Publication number
- CN1600855A CN1600855A CN 200410020239 CN200410020239A CN1600855A CN 1600855 A CN1600855 A CN 1600855A CN 200410020239 CN200410020239 CN 200410020239 CN 200410020239 A CN200410020239 A CN 200410020239A CN 1600855 A CN1600855 A CN 1600855A
- Authority
- CN
- China
- Prior art keywords
- polyvinyl alcohol
- cellulase
- solution
- film
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Nonwoven Fabrics (AREA)
Abstract
This invention relates to fixed type cellulase polyvinyl alcohol nanometer super fine fibrous membrane prepn. method. It is composed of (by wt.%): 80-99 polyvinyl alcohol and 1-20 cellulase to form the membrane which thickness is 10-50 num with average 200 nm fiber. The procedures are as follows: polyvinyl alcohol with molecular wt. 60 thousands-100 thousands is dissolved in acetic acid buffer solution to form 5-8% solution, then proceeding static textile method to form a membrane, then proceeding valeric dialdehyde vapor crosslinking to produce the product. Said fixed type cellulase membrane material is used for degradation of chitin, with high biological activity of enzyme and it can be used for several times. Its production process is simple.
Description
Technical field
The present invention relates to polyvinyl alcohol nano superfine fibre film of a kind of immobilized cellulase and preparation method thereof, belong to the immobilization technology of enzyme.
Background technology
Electrostatic spinning is polymers soln or melt form ultra-fine fibre by means of the high pressure static electricity effect a spinning technique.Can prepare the ultra-fine fibre that diameter is 3nm-1 μ m by electrostatic spinning, and can directly be gathered into superfine fibre film (Zheng M, Zhang Y Z, Kotaki M, Ramakrishna S.A review on polymernanofibers by electrospinning and their applications in nanocomposites.CompositesScience and Technology, 2003,63,2223-2253).Owing to have the advantages that specific surface area is big, porosity is high, with the carrier of electrostatic spinning ultra-fine fibre as immobilized enzyme, helping enzyme-to-substrate fully contacts, reduce sterically hindered, thereby vast potential for future development (Xie J B, You L.Ultra-high surfacefibrous membranes from electrospinning of natural proteins:casein and lipase enzyme.Journal of Materials Science, 2003 are arranged, 38,2125-133).
Enzyme is a kind of protein with catalytic capability that is produced by cell, can be under conditions such as normal temperature, normal pressure, pH neutrality highly single-minded and effectively catalytic substrate react.The immobilization of enzyme is exactly the treatment process by chemistry or physics, and original water miscible enzyme is combined or the suppressed by vector embedding with solid-state water insoluble carrier.Immobilized enzyme is compared with water-soluble enzyme, have very easily with immobilization enzyme-to-substrate and product separately, can repeated use, advantage such as good stability, reaction process be controlled (Luo Guimin. " enzyme engineering ". Beijing: Chemical Industry Press, 1-81).
Polyvinyl alcohol (PVA) is the semicrystalline polymeric of a kind of chemistry and good thermal stability, is the white powder resin, is to be formed by the Vinyl Acetate Copolymer hydrolysis.It is gas chemical industry's an important derived product, and a large amount of strongly hydrophilic oh groups are arranged in its molecule, is to have water miscible synthetic polymer.PVA also is widely used in aspects such as spinning sizing agent, coating, tackiness agent except as the vinylon raw material production fiber.In addition owing to have higher biocompatibility and a nontoxicity, PVA also have at aspects such as medicine, makeup, food important use (Yan Ruixuan. " water-soluble polymer ". Beijing: Chemical Industry Press, 2001,42-70).
With PVA is raw material, can obtain the nanometer ultra-fine fibre that mean diameter is 200nm by electrostatic spinning, as the carrier of lytic enzymes such as fixed enzyme vector, especially cellulase, does not see research report or patent of invention.
Summary of the invention
The object of the present invention is to provide polyvinyl alcohol nano superfine fibre film of a kind of immobilized cellulase and preparation method thereof, the mould material of this immobilized cellulase is as the degraded of chitosan, but have advantages such as bioenzyme activity height and repeated multiple times use, its preparation process is simple.
For achieving the above object, the present invention is realized by following technical proposals.A kind of film of polyvinyl alcohol nano ultra-fine fibre of immobilized cellulase, it is characterized in that this mould material is for being that 80～99% polyvinyl alcohol and mass percent are that 1～20% cellulase is formed, mean diameter is that the fibroplastic thickness of 200nm is the film of 10 μ m～50 μ m by mass percent.
The preparation method of the film of the polyvinyl alcohol nano ultra-fine fibre of said fixing cellulase, it is characterized in that comprising following process: with relative molecular mass is that 60,000～100,000 polyvinyl alcohol is dissolved in the hac buffer, be made into concentration and be 5～8% solution, be 1/99～20/80 in solution, to add cellulase by cellulase/polyvinyl alcohol mass ratio then, the spinning solution for preparing is injected in the electrostatic spinning apparatus, at voltage is 5kV～30kV, solution flow is 0.1mL/h～0.5mL/h, receiving range is electrostatic spinning film forming material under the condition of 5cm～25cm, and is promptly crosslinked through the glutaraldehyde steam.
The invention has the advantages that the mould material of this immobilized cellulase is used as the degraded of chitosan, but have advantages such as bioenzyme activity height and repeated multiple times use, its preparation process is simple.
Embodiment
Embodiment 1:
Get the polyvinyl alcohol (polymerization degree is 1750 ± 50, alcoholysis degree be 98%) of 0.6g, under magnetic agitation and under the heating condition, be dissolved in the 9mL hac buffer (pH=4.6); Extracting cellulose enzyme 0.03g is dissolved in the 1mL hac buffer (pH=4.6).Then, two kinds of mixed transparent spinning solutions of 10mL that form.In having the 20mL syringe that internal diameter is the flat lancet head of 0.8mm, inject spinning solution, syringe is fixed on the micro-injection pump, the vertical metal receiving screen of placing ground connection at distance syringe needle 10cm place.Syringe needle being linked to each other with the high-voltage DC power supply of 10kV, set the 0.2mL/h flow and carry out electrostatic spinning, is that the thickness that the fiber of 200nm constitutes is the film of 25 μ m to collect mean diameter behind 12～14h on receiving screen, contains a spot of fusiformis pearl in the ultra-fine fibre.Superfine fibre film is taken off from receiving screen, placed the container top of 25% glutaraldehyde water solution, place 1h under 37 ℃, normal pressure, make it crosslinked, promptly obtain the PVA immobilized cellulase, the activity of this immobilized enzyme is 136.3U/g.
Embodiment 2:
Get the polyvinyl alcohol (polymerization degree is 1750 ± 50, alcoholysis degree be 98%) of 0.7g, under magnetic agitation and under the heating condition, be dissolved in the 8mL hac buffer (pH=4.6); Extracting cellulose enzyme 0.02g is dissolved in the 1mL hac buffer (pH=4.6).Then, two kinds of mixed transparent spinning solutions of 10mL that form.In having the 20mL syringe that internal diameter is the flat lancet head of 0.8mm, inject spinning solution, syringe is fixed on the micro-injection pump, the vertical metal receiving screen of placing ground connection at distance syringe needle 10cm place.Syringe needle is linked to each other with the high-voltage DC power supply of 10kV, set the 0.2mL/h flow and carry out electrostatic spinning,, contain a spot of fusiformis pearl in the ultra-fine fibre to be superfine fibre film about 200nm collecting diameter on the receiving screen behind 12～14h.Superfine fibre film is taken off from receiving screen, placed the container top of 25% glutaraldehyde water solution, place 1h under 37 ℃, normal pressure, make it crosslinked, promptly obtain the PVA immobilized cellulase, the activity of this immobilized enzyme is 149.2U/g.。
Claims (2)
1, a kind of polyvinyl alcohol nano superfine fibre film of immobilized cellulase is characterized in that: this mould material is for being that 80～99% polyvinyl alcohol and mass percent are that 1～20% cellulase is formed, mean diameter is that the fibroplastic thickness of 200nm is the film of 10 μ m～50 μ m by mass percent.
2, a kind of preparation method who presses the polyvinyl alcohol nano superfine fibre film of the described immobilized cellulase of claim 1, it is characterized in that comprising following process: with relative molecular mass is that 60,000～100,000 polyvinyl alcohol is dissolved in that to be made into concentration in the hac buffer be 5～8% solution, be 1/99～20/80 in solution, to add enzyme by cellulase/polyvinyl alcohol mass ratio then, the spinning solution for preparing is injected in the electrostatic spinning apparatus, at voltage is 5kV～30kV, solution flow is 0.1mL/h～0.5mL/h, receiving range is an electrostatic spinning film forming material under the condition of 5cm～25cm, and crosslinked with the steam of glutaraldehyde water solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100202391A CN100340663C (en) | 2004-08-06 | 2004-08-06 | Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100202391A CN100340663C (en) | 2004-08-06 | 2004-08-06 | Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1600855A true CN1600855A (en) | 2005-03-30 |
| CN100340663C CN100340663C (en) | 2007-10-03 |
Family
ID=34663187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100202391A Expired - Fee Related CN100340663C (en) | 2004-08-06 | 2004-08-06 | Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100340663C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100363542C (en) * | 2006-05-16 | 2008-01-23 | 苏州大学 | Polyvinyl alcohol electrostatic spinning solution |
| CN100371372C (en) * | 2006-03-22 | 2008-02-27 | 浙江大学 | Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof |
| CN101671665B (en) * | 2009-10-27 | 2010-12-15 | 北京师范大学 | Method for preparing immobilized laccase in electrospun fiber membrane |
| CN102174504A (en) * | 2011-01-14 | 2011-09-07 | 华南理工大学 | Granular carrier immobilized lipase for treating papermaking white water and preparation method thereof |
| CN102730818A (en) * | 2012-07-06 | 2012-10-17 | 北京师范大学 | Method for repairing water eutrophication pollution based on urease immobilized nanometer film |
| CN106223019A (en) * | 2016-08-22 | 2016-12-14 | 王利萍 | A kind of preparation method of cross-linking polyvinyl alcohol nano fibrous membrane |
| CN111560363A (en) * | 2020-07-16 | 2020-08-21 | 凯莱英生命科学技术(天津)有限公司 | PVA film immobilized enzyme and preparation method thereof |
| CN111876405A (en) * | 2020-08-10 | 2020-11-03 | 山东华熙海御生物医药有限公司 | Method for immobilizing enzyme on nanofiber |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2895690B2 (en) * | 1992-09-12 | 1999-05-24 | 鐘紡株式会社 | Weight loss processing method for cellulosic fiber structure |
| JPH0823974A (en) * | 1994-07-18 | 1996-01-30 | Kuraray Co Ltd | Production of gel holding immobilized biocatalyst |
| CN1137991C (en) * | 2000-12-15 | 2004-02-11 | 清华大学 | Process for immobilizing enzyme/microbe with polyvinyl alcohol |
-
2004
- 2004-08-06 CN CNB2004100202391A patent/CN100340663C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100371372C (en) * | 2006-03-22 | 2008-02-27 | 浙江大学 | Phospholipid modified acrylon nano fiber composite film and its preparation and immobilized enzyme thereof |
| CN100363542C (en) * | 2006-05-16 | 2008-01-23 | 苏州大学 | Polyvinyl alcohol electrostatic spinning solution |
| CN101671665B (en) * | 2009-10-27 | 2010-12-15 | 北京师范大学 | Method for preparing immobilized laccase in electrospun fiber membrane |
| CN102174504A (en) * | 2011-01-14 | 2011-09-07 | 华南理工大学 | Granular carrier immobilized lipase for treating papermaking white water and preparation method thereof |
| CN102730818A (en) * | 2012-07-06 | 2012-10-17 | 北京师范大学 | Method for repairing water eutrophication pollution based on urease immobilized nanometer film |
| CN102730818B (en) * | 2012-07-06 | 2013-08-14 | 北京师范大学 | Method for repairing water eutrophication pollution based on urease immobilized nanometer film |
| CN106223019A (en) * | 2016-08-22 | 2016-12-14 | 王利萍 | A kind of preparation method of cross-linking polyvinyl alcohol nano fibrous membrane |
| CN111560363A (en) * | 2020-07-16 | 2020-08-21 | 凯莱英生命科学技术(天津)有限公司 | PVA film immobilized enzyme and preparation method thereof |
| CN111876405A (en) * | 2020-08-10 | 2020-11-03 | 山东华熙海御生物医药有限公司 | Method for immobilizing enzyme on nanofiber |
| CN111876405B (en) * | 2020-08-10 | 2022-07-15 | 山东华熙海御生物医药有限公司 | Method for immobilizing enzyme on nano fiber |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100340663C (en) | 2007-10-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101798756B (en) | Method for preparing biomedical material of static self-assembly modified nano fiber | |
| Doğaç et al. | A comparative study for lipase immobilization onto alginate based composite electrospun nanofibers with effective and enhanced stability | |
| Huang et al. | Preparation and characterization of stable chitosan nanofibrous membrane for lipase immobilization | |
| JP2011056456A (en) | Method for producing bio-nanofiber | |
| CN1908039A (en) | Process for preparing chitosan nano fiber film with stable state | |
| CN103572507A (en) | Preparation method for antibiosis ultraviolet prevention silk fibroin nanofiber membrane | |
| Chen et al. | Fabrication of electrospun poly (methyl methacrylate) nanofibrous membranes by statistical approach for application in enzyme immobilization | |
| Narita et al. | Characterization of ground silk fibroin through comparison of nanofibroin and higher order structures | |
| CN1818163A (en) | Blending nanometer fibre with silk protein and vinol and production thereof | |
| CN100340663C (en) | Nano superfine fiber membrane of polyvinyl alcohol with immovable cellulase | |
| CN106480517A (en) | A kind of sodium alginate nano fiber and its scale electro spinning method for preparing and application | |
| CN111876405B (en) | Method for immobilizing enzyme on nano fiber | |
| CN107670115A (en) | Fibroin albumen/hydroxyapatite/poly-（Racemic lactic acid co caprolactones）The preparation method of composite nano-fiber membrane | |
| CN110511399A (en) | A kind of preparation method of control release type nano-cellulose antibacterial microgel | |
| Mohan et al. | Nanocellulosic materials in tissue engineering applications | |
| Kalluri et al. | Effect of electrospinning parameters on the fiber diameter and morphology of PLGA nanofibers | |
| CN112981556A (en) | Electrostatic spinning fiber collecting device and method for preparing nanofiber membrane with radial orientation structure | |
| Costa et al. | Nanopores structure in electrospun bacterial cellulose | |
| Jayakumar et al. | Perspectives of chitin and chitosan nanofibrous scaffolds in tissue engineering | |
| Zamani et al. | Response of human mesenchymal stem cells to patterned and randomly oriented poly (vinyl alcohol) nano-fibrous scaffolds surface-modified with Arg-Gly-Asp (RGD) ligand | |
| Islam et al. | Surface deposition of chitosan on wool substrate by electrospraying | |
| CN108728929A (en) | A kind of nano-fiber material and preparation method thereof of filtering heavy metal ion | |
| WO2018047905A1 (en) | ε-POLYLYSINE MICROFIBER AND FIBER STRUCTURE, AND METHOD FOR MANUFACTURING SAME | |
| El-Sherbiny et al. | Eco-friendly electrospun polymeric nanofibers-based nanocomposites for wound healing and tissue engineering | |
| CN107485578A (en) | A kind of electro-spinning for chitosan/oligopeptides biology cleawhite pack method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |