CN1596585A - Large scale liquid deep fermentation for producing brilliant bacterial mycellium powder and its polysaccbaride technology - Google Patents
Large scale liquid deep fermentation for producing brilliant bacterial mycellium powder and its polysaccbaride technology Download PDFInfo
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Abstract
A technology for preparing the mycelia, powder and polyose of armillarisiae by large-scale deep liquid fermenting includes such steps as shak-flask liquid culture, enlarge culture, stage-one seed culture, stage-two seed culture, stage-three fermenting culture to obtain mycelia in fermented liquid, high-speed stirring, concentrating, sterilizing and spray drying to obtain powder, or heating fermented liquid to 90-100 deg.C while stirring, and press filter to obtain polyose.
Description
Technical field
The present invention relates to a kind of large-scale deep liquid fermentation and produce armillaria mellea mycelium body bacterium powder and polysaccharide process thereof.
Technical background
Armilariella tabescens (Armillariella tabescens (Scop.:Fr.) Sing), English name Ringles HoneyMushroom has another name called luminous Armillariella, the easy glass umbrella that dies, genus agaricus order, dried mushroom Cordycepps, Armillariella.The flat hemispherical of cap, after gradually open and flat, the middle part is recessed, diameter 2-7 centimetre, surperficial honey gold is to dark eggshell look, and is slightly than the middle part look shallow all around, and the hair shape ramentum of closeer shallow rotten leaf look, pitchy is arranged, the edge is complete, and unconspicuous striped is arranged; The light yellowish pink of lamella, rare slightly, different in size, closely prolong life with stem; Bacterial context white is to white yellow, and the middle part is thick, and thin edge is lightly seasoned, and is good to eat.Stem is cylindrical, and long 5-12 centimetre, thick 3-8 millimeter, the shallow taupe of base portion is to dark brown grey, and the look light slightly eggshell look that is in top has the fine fleece striga at the beginning, becomes smooth when aging, and cellulosic is inner soft; The spore oval, colourless, smooth, 7.5-10 micron * 5.3-7.5 micron does not have the reaction of the starchiness of plan, spore print white.Mycelia can send out fluorescence azury.This bacterium and young mistress encircle difference and are that cap is not sticking, asepsis ring, the flat deer horn shape that is of shoestring.Be born in the stake of broad-leaved tree summer and autumn and go up or butt, cause root-rot, grow thickly.In Yunnan, there is distribution in Sichuan, Henan, Fujian, Zhejiang, Anhui, Hebei.
According to one's analysis, these bacterium 100 gram dry products contain protein 18.3 grams, fat 1.9 grams, carbohydrate 58.9 grams, 329 kilocalories of heats, 400 milligrams in phosphorus, 3.96 milligrams in vitamin b3.Mycelia of this bacterium and rhzomorph contain halimasch coumarin (being halimasch first element), ergosterol etc., and its tunning preparation has the effect of treatment cholecystitis and catarrhal jaundice.
Armilariella tabescens preparation in the market is to adopt Armilariella tabescens solid culture mode to produce, and exists the production cycle long, shortcomings such as the low and easy microbiological contamination of production efficiency.Adopt production armillaria mellea mycelium body and polysaccharide thereof that tertiary liquid fermentation technology can be quick, a large amount of, greater advantage is arranged than solid fermentation.Have not yet to see the report that adopts tertiary liquid fermentation technology large-scale production armillaria mellea mycelium body bacterium powder and polysaccharide thereof.
Summary of the invention
The objective of the invention is to produce armillaria mellea mycelium body bacterium powder and polysaccharide is target, invents a kind of armillaria mellea mycelium body bacterium powder of Cheap highly effective and the large-scale deep liquid fermentation process of polysaccharide thereof, for from now on commercial application lays the first stone.
Realize technical scheme of the present invention: with the Armilariella tabescens fungi is starting strain, adopts slant strains to carry out liquid shaking bottle cultivation, liquid shaking bottle enlarged culture, first order seed cultivation, secondary seed cultivation and three grade fermemtation and cultivates.This bacterial strain is available from Jinxiang, Shandong fungal studies institute.
Realize that concrete steps of the present invention are as follows:
(1) takes out slant strains and insert in the fresh slant medium of preparing, cultivation temperature 28-30 ℃, cultivated 150-180 hour.
(2) slant strains in the step 1 is transferred the shaking in the bottle of shake-flask culture base is housed, 28-30 ℃ of cultivation, rotating speed 120-180rpm/min cultivates the enlarged culture of shaking bottle after 96-120 hour.
(3) bottle bacterial classification that shakes in the step 2 is transferred and carried out enlarged culture again into the bottle that shakes that shakes bottle enlarged culture base is housed, 28-30 ℃ of cultivation, rotating speed 120-180rpm/min cultivates after 30-60 hour the access first class seed pot and cultivates.
(4) bottle bacterial classification that shakes in the step 3 is transferred one and is equipped with in the fermentation tank of first order seed medium, inoculum concentration is 4-10%, keep warm 28-30 ℃ in jar, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 90-150 hour, and the fermentation of secondary seed culture tank is gone in switching then.
(5) bacterial classification in the first class seed pot is transferred one be equipped with in the fermentation tank of secondary seed medium, inoculum concentration is 4-10%, keep warm 28-30 ℃ in jar, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 40-60 hour, and the fermentation of three grade fermemtation jar is gone in switching then.
(6) bacterial classification in the secondary seed jar is transferred one be equipped with in the fermentation tank of three grade fermemtation medium, inoculum concentration is 4-10%, keeps jar warm 28-30 ℃, tank pressure 0.5-0.9kPa, stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 100-150 hour.Be reduced to 1.0% at content of reducing sugar and put jar when following.
Obtain the bacterium powder as need, then end zymotic fluid in fermentation tank to smash at a high speed 1 hour greater than the rotating speed of 300rpm/min, then it is concentrated, treat that solid content reaches 15-20% after, in 120 ℃ of sterilization 20-30min, adopt aseptic atomized drying to get the bacterium powder.
Obtain its polysaccharide as need, then end of a period zymotic fluid temperature is heated to 90-100 ℃, mixing speed is 200-250rpm/min, keeps 2-4 hour, with pump zymotic fluid is transported in the basin, obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide through plate compression.
The prescription of slant medium is (g/100ml) among the present invention: potato 10-30, glucose 1-3, agar 1.5-2.5, add water be settled to volume required, pH value nature.
Shake-flask culture among the present invention, shake a bottle enlarged culture, first order seed is cultivated and secondary seed is cultivated medium is formed is (g/100ml): glucose 1-3, yeast extract 0.3-0.7, peptone 0.1-0.3, NaCl 0.05-0.2, KH
2PO
40.05-0.2, MgSO
40.03-0.1, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
It is (g/100ml) that three grade fermemtation medium described in the present invention is formed: glucose 2-4, yeast extract 0.5-1.5, peptone 0.3-0.7, starch 0.5-2, growth hormone 0.3-0.7, NaCl 0.05-0.15, (NH
4)
2SO
40.05-0.2, KH
2PO
40.1-0.4, MgSO
40.05-0.15, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
The shake-flask culture base is 1: 5 with shaking bottle volumetric ratio in the step 2 of the present invention and 3.
The volume of first class seed pot is 100-300L in the step 4 of the present invention, and the first order seed medium that is equipped with in this first class seed pot should be 50-180L mutually; The volume of secondary seed jar is 1000-5000L in the step 5, and the fermentation medium that is equipped with in this fermentation tank should be 500-3000L mutually; The volume of three grade fermemtation jar is 10000L-15000L in the step 6, and the fermentation medium that is equipped with in this fermentation tank should be 5000L-9000L mutually.
Jar standard of putting of the present invention is that zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to below 1.0%, and microscopy finds that mycelium senesces.
The mensuration of mycelium dry weight adopts oven drying method, and polysaccharide extracts and adopts ethanol precipitation, adopts the method for document; Feng Huiqin, Yang Qingyao, waste can, Shen Yong: grifola frondosus liquid culture mycelia produces STUDY ON POLYSACHAROSE.The edible mushroom journal, 2000,7 (2): 5-10.
Beneficial effect of the present invention: the present invention is applicable to large-scale deep liquid fermentation production armillaria mellea mycelium body bacterium powder and polysaccharide thereof, solve large-scale production and cultivated a difficult problem of amplifying, significant for the development of from now on suitability for industrialized production and relevant application industry.The tunning color that the present invention obtains after fermentation ends is brown, has the fragrance of approximate honey, and dry mycelium powder is brown.Mycelium is counted 2-3% with dry weight in the zymotic fluid.The bacterium powder that the present invention produced can be used as the raw material of producing tablet, capsule etc.; The polysaccharide of being produced can be used as the raw material of producing various liquid preparations, can be used as the raw material of solid pharmaceutical preparations such as producing tablet, capsule after the drying.
Embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
Embodiment 1:
Inoculation armillaria mellea mycelium body in slant medium, 28 ℃ of cultivation temperature, incubation time 180 hours.Fresh slant strains access is equipped with in the 500ml triangular flask of 100ml medium, and 28 ℃ of shaking tables were cultivated shaking speed 150rpm/min 100 hours.Then gained is shaken transfer 500ml triangle that the 100ml medium is housed of bottle bacterial classification and shake and carry out enlarged culture in the bottle, 28 ℃ of shaking tables were cultivated shaking speed 150rpm/min 48 hours.During the bacterial classification 2200ml that triangular flask is enlarged transfers the 100L first class seed pot that the 50L seed culture medium is housed then, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 100 hours.The first class seed pot bacterial classification is transferred in the 1500L secondary seed culture tank that the 800L seed culture medium is housed, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 50 hours.Secondary seed jar bacterial classification is transferred in the 15000L three grade fermemtation jar that the 8000L fermentation medium is housed, keep 28 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, mycelium counts 2.5% with dry weight in 150 hours zymotic fluids of fermentation time, and polyoses content is 15% in the dry bacterium powder.
To form be (g/100ml) to slant medium in the present embodiment: potato 20, and glucose 2, agar 2 adds water and is settled to volume requiredly, and the pH value is a nature.
Shake-flask culture in the present embodiment, shake a bottle enlarged culture, first order seed is cultivated and secondary seed is cultivated medium is formed is (g/100ml): glucose 2, yeast extract 0.5, peptone 0.2, NaCl 0.1, KH
2PO
40.1, MgSO
40.05, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
It is (g/100ml) that three grade fermemtation medium described in the present embodiment is formed: glucose 3, and yeast extract 1.0, peptone 0.5, starch 1, growth hormone 0.5, NaCl 0.1, (NH
4)
2SO
40.1, KH
2PO
40.2, MgSO
40.1, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
The fermentation ends standard is in the present embodiment: zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to below 1.0%, and microscopy finds that mycelium senesces.
The method of obtaining the bacterium powder is: the end of a period zymotic fluid was smashed at a high speed 1 hour with the rotating speed of 320rpm/min in fermentation tank, then it was concentrated, treat that solid content reaches 18% after, in 120 ℃ of sterilization 30min, adopt aseptic atomized drying to get the bacterium powder.
The method of obtaining polysaccharide is: after fermentation of the present invention stops, temperature is heated to 90 ℃, and mixing speed is 220rpm/min, keeps 3 hours, with pump zymotic fluid is transported in the basin, through plate compression, concentrating filter liquor, making last volume is 1/5 of stoste, adding an amount of ethanol makes concentration of alcohol reach 75%, alcohol was analysed 24 hours, got the precipitate with deionized water dissolving, and atomized drying obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide.
Embodiment 2:
Inoculation armillaria mellea mycelium body in slant medium, 28.5 ℃ of cultivation temperature, incubation time 168 hours.Fresh slant strains access is equipped with in the 500ml triangular flask of 100ml medium, and 28.5 ℃ of shaking tables were cultivated shaking speed 150rpm/min 96 hours.Then gained is shaken transfer 500ml triangle that the 100ml medium is housed of bottle bacterial classification and shake and carry out enlarged culture in the bottle, 28.5 ℃ of shaking tables were cultivated shaking speed 150rpm/min 56 hours.During the bacterial classification 2500ml that triangular flask is enlarged transfers the 100L first class seed pot that the 60L seed culture medium is housed then, keep 28.5 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 110rpm/min, throughput 1: 0.5v/v.m, fermentation time 98 hours.The first class seed pot bacterial classification is transferred in the 1500L secondary seed culture tank that the 800L fermentation medium is housed, keep 28.5 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 110rpm/min, throughput 1: 0.5v/v.m, fermentation time 56 hours.Secondary seed jar bacterial classification is transferred in the 15000L three grade fermemtation jar that the 9000L fermentation medium is housed, keep 28.5 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 110rpm/min, throughput 1: 0.5v/v.m, mycelium counts 2.8% with dry weight in 132 hours zymotic fluids of fermentation time, and polyoses content is 17% in the dry bacterium powder.
To form be (g/100ml) to slant medium in the present embodiment: potato 20, and glucose 2, agar 2 adds water and is settled to volume requiredly, and the pH value is a nature.
Shake-flask culture in the present embodiment, shake a bottle enlarged culture, first order seed is cultivated and secondary seed is cultivated medium is formed is (g/100ml): glucose 2, yeast extract 0.5, peptone 0.2, NaCl 0.1, KH
2PO
40.1, MgSO
40.05, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
It is (g/100ml) that three grade fermemtation medium described in the present embodiment is formed: glucose 4, and yeast extract 1.5, peptone 0.5, starch 1.5, growth hormone 0.5, NaCl 0.1, (NH
4)
2SO
40.1, KH
2PO
40.2, MgSO
40.1, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
The fermentation ends standard is in the present embodiment: zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to below 1.0%, and microscopy finds that mycelium senesces.
The method of obtaining the bacterium powder is: the end of a period zymotic fluid was smashed at a high speed 1 hour with the rotating speed of 320rpm/min in fermentation tank, then it was concentrated, treat that solid content reaches 20% after, in 120 ℃ of sterilization 30min, adopt aseptic atomized drying to get the bacterium powder.
The method of obtaining polysaccharide is: after fermentation of the present invention stops, temperature is heated to 100 ℃, and mixing speed is 220rpm/min, keeps 3 hours, with pump zymotic fluid is transported in the basin, through plate compression, concentrating filter liquor, making last volume is 1/5 of stoste, adding an amount of ethanol makes concentration of alcohol reach 75%, alcohol was analysed 24 hours, got the precipitate with deionized water dissolving, and atomized drying obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide.
Embodiment 3:
Inoculation armillaria mellea mycelium body in slant medium, 29 ℃ of cultivation temperature, incubation time 160 hours.Fresh slant strains access is equipped with in the 500ml triangular flask of 100ml medium, and 29 ℃ of shaking tables were cultivated shaking speed 150rpm/min 96 hours.Then gained is shaken transfer 500ml triangle that the 100ml medium is housed of bottle bacterial classification and shake and carry out enlarged culture in the bottle, 29 ℃ of shaking tables were cultivated shaking speed 150rpm/min 44 hours.During the bacterial classification 2500ml that triangular flask is enlarged transfers the 100L first class seed pot that the 50L seed culture medium is housed then, keep 29 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 96 hours.The first class seed pot bacterial classification is transferred in the 1000L secondary seed culture tank that the 500L fermentation medium is housed, keep 29 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 40 hours.Secondary seed jar bacterial classification is transferred in the 10000L three grade fermemtation jar that the 5000L fermentation medium is housed, keep 29 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, mycelium counts 2.5% with dry weight in 120 hours zymotic fluids of fermentation time, and polyoses content is 13% in the dry bacterium powder.
To form be (g/100ml) to slant medium in the present embodiment: potato 20, and glucose 2, agar 2 adds water and is settled to volume requiredly, and the pH value is a nature.
Shake-flask culture in the present embodiment, shake a bottle enlarged culture, first order seed is cultivated and secondary seed is cultivated medium is formed is (g/100ml): glucose 2, yeast extract 0.5, peptone 0.2, NaCl 0.1, KH
2PO
40.1, MgSO
40.05, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
It is (g/100ml) that three grade fermemtation medium described in the present embodiment is formed: glucose 3, and yeast extract 1.2, peptone 0.5, starch 0.9, growth hormone 0.4, NaCl 0.1, (NH
4)
2SO
40.1, KH
2PO
40.2, MgSO
40.1, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
The fermentation ends standard is in the present embodiment: zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to below 1.0%, and microscopy finds that mycelium senesces.
The method of obtaining the bacterium powder is: the end of a period zymotic fluid was smashed at a high speed 1 hour with the rotating speed of 320rpm/min in fermentation tank, then it was concentrated, treat that solid content reaches 16% after, in 120 ℃ of sterilization 30min, adopt aseptic atomized drying to get the bacterium powder.
The method of obtaining polysaccharide is: after fermentation of the present invention stops, temperature is heated to 100 ℃, and mixing speed is 220rpm/min, keeps 3 hours, with pump zymotic fluid is transported in the basin, through plate compression, concentrating filter liquor, making last volume is 1/5 of stoste, adding an amount of ethanol makes concentration of alcohol reach 75%, alcohol was analysed 24 hours, got the precipitate with deionized water dissolving, and atomized drying obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide.
Embodiment 4:
Inoculation armillaria mellea mycelium body in slant medium, 30 ℃ of cultivation temperature, incubation time 150 hours.Fresh slant strains access is equipped with in the 500ml triangular flask of 100ml medium, and 30 ℃ of shaking tables were cultivated shaking speed 150rpm/min 94 hours.Then gained is shaken transfer 500ml triangle that the 100ml medium is housed of bottle bacterial classification and shake and carry out enlarged culture in the bottle, 30 ℃ of shaking tables were cultivated shaking speed 150rpm/min 44 hours.During the bacterial classification 2600ml that triangular flask is enlarged transfers the 100L first class seed pot that the 50L seed culture medium is housed then, keep 30 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 92 hours.The first class seed pot bacterial classification is transferred in the 1000L secondary seed culture tank that the 500L fermentation medium is housed, keep 30 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, fermentation time 40 hours.Secondary seed jar bacterial classification is transferred in the 10000L three grade fermemtation jar that the 5000L fermentation medium is housed, keep 30 ℃ of jar temperature, tank pressure 0.7kPa, stir speed (S.S.) 100rpm/min, throughput 1: 0.5v/v.m, mycelium counts 2.2% with dry weight in 100 hours zymotic fluids of fermentation time, and polyoses content is 12% in the dry bacterium powder.
To form be (g/100ml) to slant medium in the present embodiment: potato 20, and glucose 2, agar 2 adds water and is settled to volume requiredly, and the pH value is a nature.
Shake-flask culture in the present embodiment, shake a bottle enlarged culture, first order seed is cultivated and secondary seed is cultivated medium is formed is (g/100ml): glucose 2, yeast extract 0.5, peptone 0.2, NaCl 0.1, KH
2PO
40.1, MgSO
40.05, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
It is (g/100ml) that three grade fermemtation medium described in the present embodiment is formed: glucose 2.2, and yeast extract 1.4, peptone 0.5, starch 0.9, growth hormone 0.4, NaCl 0.1, (NH
4)
2SO
40.1, KH
2PO
40.2, MgSO
40.1, adding water and be settled to volume requiredly, the pH value is 4.5-5.0.
The fermentation ends standard is in the present embodiment: zymotic fluid begins the thickness that becomes, and content of reducing sugar is reduced to below 1.0%, and microscopy finds that mycelium senesces.
The method of obtaining the bacterium powder is: the end of a period zymotic fluid was smashed at a high speed 1 hour with the rotating speed of 320rpm/min in fermentation tank, then it was concentrated, treat that solid content reaches 15% after, in 120 ℃ of sterilization 30min, adopt aseptic atomized drying to get the bacterium powder.
The method of obtaining polysaccharide is: after fermentation of the present invention stops, temperature is heated to 100 ℃, and mixing speed is 220rpm/min, keeps 3 hours, with pump zymotic fluid is transported in the basin, through plate compression, concentrating filter liquor, making last volume is 1/5 of stoste, adding an amount of ethanol makes concentration of alcohol reach 75%, alcohol was analysed 24 hours, got the precipitate with deionized water dissolving, and atomized drying obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide.
Claims (4)
1, a kind of Armilariella tabescens liquid deep layer fermenting production technology, it is characterized in that: with the Armilariella tabescens fungi is starting strain, adopt that slant strains carries out that liquid shaking bottle cultivations, liquid shaking bottle enlarged culture, first order seed are cultivated, secondary seed cultivation and three grade fermemtation cultivate armillaria mellea mycelium body zymotic fluid; Obtain its bacterium powder as need, then zymotic fluid is smashed at a high speed in fermentation tank, after concentrating sterilization, aseptic atomized drying gets the bacterium powder; Or after the fermentation ends end of a period zymotic fluid is continued to be heated to uniform temperature, and stirring and keep certain hour, zymotic fluid obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide through plate compression;
Shake-flask culture, shake a bottle enlarged culture, first order seed are cultivated and secondary seed is cultivated medium and form and count: glucose 1-3, yeast extract 0.3-0.7, peptone 0.1-0.3, NaCl 0.05-0.2, KH with g/100ml
2PO
40.05-0.2, MgSO
40.03-0.1, adding water and be settled to volume requiredly, the pH value is 4.5-5.0;
The condition of culture of shake-flask culture is cultivation temperature 28-30 ℃, and rotating speed 120-180rpm/min cultivated 96-120 hour, and switching is gone into to shake bottle and carried out enlarged culture then;
The condition of culture that shakes bottle enlarged culture is cultivation temperature 28-30 ℃, and rotating speed 120-180rpm/min cultivated 30-60 hour, and the first class seed pot cultivation is gone in switching then, and inoculum concentration is 4-10%;
The condition of culture that first order seed is cultivated is cultivation temperature 28-30 ℃, tank pressure 0.5-0.9kPa, and stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 90-150 hour, and the cultivation of secondary seed jar is gone in switching then, and inoculum concentration is 4-10%;
The condition of culture that secondary seed is cultivated is cultivation temperature 28-30 ℃, tank pressure 0.5-0.9kPa, and stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 40-60 hour, and the cultivation of three grade fermemtation jar is gone in switching then, and inoculum concentration is 4-10%;
Three grade fermemtation medium composition is counted with g/100ml: glucose 2-4, yeast extract 0.5-1.5, peptone 0.3-0.7, starch 0.5-2, growth hormone 0.3-0.7, NaCl 0.05-0.15, (NH
4)
2SO
40.05-0.2, KH
2PO
40.1-0.4, MgSO
40.05-0.15, adding water and be settled to volume requiredly, the pH value is 4.5-5.0; Condition of culture is cultivation temperature 28-30 ℃, tank pressure 0.5-0.9kPa, and stir speed (S.S.) 80-150rpm/min, throughput 1: 0.3-0.5v/v.m fermented 100-150 hour;
Three grade fermemtation is cultivated, and can select the 10000L-15000L fermentation tank to carry out fermented and cultured.
2, Armilariella tabescens liquid deep layer fermenting production technology according to claim 1 is characterized in that: being starting strain available from Jinxiang, Shandong fungal studies institute Armilariella tabescens fungi.
3, Armilariella tabescens liquid deep layer fermenting production technology according to claim 1, it is characterized in that: obtain the bacterium powder as need, then end zymotic fluid in fermentation tank to smash at a high speed 1 hour greater than the rotating speed of 300rpm/min, then it is concentrated, after treating that solid content reaches 15-20%, in 120 ℃ of sterilization 20-30min, adopt aseptic atomized drying to get the bacterium powder.
4, Armilariella tabescens liquid deep layer fermenting production technology according to claim 1, it is characterized in that: obtain its polysaccharide as need, then end of a period zymotic fluid temperature is heated to 90-100 ℃, mixing speed is 200-250rpm/min, kept 2-4 hour, with pump zymotic fluid is transported in the basin, obtains Armillaria mella tabescens (Scop.ex Fr.) Sing. Polysaccharide through plate compression.
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| CN107056513A (en) * | 2017-06-27 | 2017-08-18 | 山东昆仲知识产权代理有限公司 | Microbial bacteria agent and process for producing same |
| CN110037202A (en) * | 2019-04-30 | 2019-07-23 | 广州保泰特生物科技有限公司 | A kind of modified fish meal and its preparation method and application |
| CN111253496A (en) * | 2020-01-21 | 2020-06-09 | 安徽大学 | Armillariella tabescens mycelium polysaccharide with blood sugar reducing effect |
| CN115010822A (en) * | 2022-06-09 | 2022-09-06 | 合肥诚志生物制药有限公司 | Armillariella tabescens mycelium polysaccharide and its application |
-
2004
- 2004-08-12 CN CN 200410041692 patent/CN1281113C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313594C (en) * | 2005-09-19 | 2007-05-02 | 王永年 | Preparation method of epiphyte cellular fluid for dual purpose of edible and medicinal use |
| CN102351580A (en) * | 2011-10-14 | 2012-02-15 | 福建农林大学 | Method for preparing black fungus nutrient solution by utilizing food processing residues |
| CN102351580B (en) * | 2011-10-14 | 2013-01-09 | 福建农林大学 | Method for preparing black fungus nutrient solution by utilizing food processing residues |
| CN107056513A (en) * | 2017-06-27 | 2017-08-18 | 山东昆仲知识产权代理有限公司 | Microbial bacteria agent and process for producing same |
| CN110037202A (en) * | 2019-04-30 | 2019-07-23 | 广州保泰特生物科技有限公司 | A kind of modified fish meal and its preparation method and application |
| CN111253496A (en) * | 2020-01-21 | 2020-06-09 | 安徽大学 | Armillariella tabescens mycelium polysaccharide with blood sugar reducing effect |
| CN115010822A (en) * | 2022-06-09 | 2022-09-06 | 合肥诚志生物制药有限公司 | Armillariella tabescens mycelium polysaccharide and its application |
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|---|---|
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