CN1592791B - 肌醇六磷酸酶,其编码核酸及其制造和使用方法 - Google Patents

肌醇六磷酸酶,其编码核酸及其制造和使用方法 Download PDF

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Publication number
CN1592791B
CN1592791B CN028103270A CN02810327A CN1592791B CN 1592791 B CN1592791 B CN 1592791B CN 028103270 A CN028103270 A CN 028103270A CN 02810327 A CN02810327 A CN 02810327A CN 1592791 B CN1592791 B CN 1592791B
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Prior art keywords
nucleic acid
sequence
phytase
polypeptide
mutagenesis
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Expired - Lifetime
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CN028103270A
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Chinese (zh)
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CN1592791A (zh
Inventor
J·M·肖特
K·克雷茨
K·A·格雷
N·R·巴顿
J·B·加勒特
E·奥多诺休
E·J·马瑟
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BASF Enzymes LLC
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Verenium Corp
Diversa Corp
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    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00Methods for preparing or baking dough
    • A21D8/02Methods for preparing dough; Treating dough prior to baking
    • A21D8/04Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/30Removing undesirable substances, e.g. bitter substances
    • A23L11/33Removing undesirable substances, e.g. bitter substances using enzymes; Enzymatic transformation of pulses or legumes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/104Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
    • A23L7/107Addition or treatment with enzymes not combined with fermentation with microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030083-Phytase (3.1.3.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/03Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/030264-Phytase (3.1.3.26), i.e. 6-phytase
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Agronomy & Crop Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Beans For Foods Or Fodder (AREA)
CN028103270A 2001-05-24 2002-05-24 肌醇六磷酸酶,其编码核酸及其制造和使用方法 Expired - Lifetime CN1592791B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/866,379 US6855365B2 (en) 1997-08-13 2001-05-24 Recombinant bacterial phytases and uses thereof
US09/866,379 2001-05-24
PCT/US2002/016482 WO2002095003A2 (en) 2001-05-24 2002-05-24 Phytases, nucleic acids encoding them and methods for making and using them

Publications (2)

Publication Number Publication Date
CN1592791A CN1592791A (zh) 2005-03-09
CN1592791B true CN1592791B (zh) 2012-09-05

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CN028103270A Expired - Lifetime CN1592791B (zh) 2001-05-24 2002-05-24 肌醇六磷酸酶,其编码核酸及其制造和使用方法

Country Status (9)

Country Link
US (1) US6855365B2 (enExample)
EP (1) EP1478769A4 (enExample)
JP (1) JP2005505254A (enExample)
KR (1) KR20040099097A (enExample)
CN (1) CN1592791B (enExample)
BR (2) BR122014019327B1 (enExample)
CA (1) CA2441666A1 (enExample)
IN (1) IN2004DE03129A (enExample)
WO (1) WO2002095003A2 (enExample)

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US7432097B2 (en) * 1997-08-13 2008-10-07 Verenium Corporation Phytases, nucleic acids encoding them and methods of making and using them
US6720014B1 (en) 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US7078035B2 (en) * 1997-08-13 2006-07-18 Diversa Corporation Phytases, nucleic acids encoding them and methods for making and using them
US6183740B1 (en) * 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
ES2597503T3 (es) * 2001-10-31 2017-01-19 Huvepharma Eood Alimento para animales que contiene fitasa y método
TWI262083B (en) * 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
TW200305430A (en) * 2001-12-28 2003-11-01 Syngenta Participations Ag Thermotolerant phytase for animal feed
DE60335195D1 (de) * 2002-08-12 2011-01-13 Danisco Us Inc Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon
DE60335497D1 (de) 2002-09-13 2011-02-03 Cornell Res Foundation Inc Ergillus-phytasen
CA2413240A1 (en) * 2002-11-29 2004-05-29 Mcn Bioproducts Inc. Purification of inositol from plant materials
WO2004085638A1 (en) * 2003-03-25 2004-10-07 Republic Of National Fisheries Research And Development Institute Phytase produced from citrobacter braakii
AU2004263844A1 (en) * 2003-07-07 2005-02-17 Syngenta Participations Ag Reagents, methods and kits for detecting feed enzymes
US7374786B2 (en) 2004-01-09 2008-05-20 Biosys Corporation Bioimmune-aggressin composition for suppression of xanthomonad infections in agriculture crops
US20060073193A1 (en) * 2004-09-27 2006-04-06 Novozymes A/S Enzyme granules
WO2006034710A1 (en) 2004-09-27 2006-04-06 Novozymes A/S Enzyme granules
DE102004050410A1 (de) 2004-10-15 2006-06-08 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und dieses codierende Nucleotidsequenz
ES2679282T3 (es) 2004-10-22 2018-08-23 Revivicor Inc. Porcinos transgénicos que carecen de cadena ligera de inmunoglobulina endógena
WO2006081825A1 (en) * 2005-02-04 2006-08-10 University Of Aarhus A method for recycling important nutritional elements from waste
US7658922B2 (en) * 2005-06-24 2010-02-09 Ab Enzymes Gmbh Monoclonal antibodies, hybridoma cell lines, methods and kits for detecting phytase
GB0600913D0 (en) 2006-01-17 2006-02-22 Syngenta Ltd Improvements in or relating to organic compounds
WO2007136428A2 (en) 2006-01-19 2007-11-29 Solazyme, Inc. Nutraceutical compositions from microalgae and related methods of production and administration
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
WO2007128160A1 (en) * 2006-04-30 2007-11-15 Feed Research Institute Chinese Academy Of Agricultural Sciences Cloning and expression of a novel phytase
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2617823B1 (en) * 2006-09-21 2015-07-01 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
DE102006053059A1 (de) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
WO2008116878A1 (en) * 2007-03-26 2008-10-02 Novozymes A/S Hafnia phytase
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
ES2835955T3 (es) * 2008-04-18 2021-06-23 Danisco Us Inc Variantes de fitasa de Buttiauxella sp.
US8367392B2 (en) * 2008-09-05 2013-02-05 Transalgae Ltd. Genetic transformation of algal and cyanobacteria cells by microporation
WO2010027505A2 (en) * 2008-09-05 2010-03-11 Doron Eisenstadt Decreasing rubisco content of algae and cyanobacteria cultivated in high carbon dioxide
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
KR101336213B1 (ko) * 2008-11-14 2013-12-05 후지안 후다 바이오테크 코퍼레이션 리미티드 열내성 비 k12 에스체리치아 콜리 피타아제 및 그 제조
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CN102559632B (zh) * 2010-12-22 2013-06-26 武汉新华扬生物股份有限公司 一种在酸性范围内催化活性提高的优化改良的大肠杆菌植酸酶appa-m及其基因和应用
CN102629304B (zh) * 2012-04-05 2015-02-04 天津大学 基于基因组尺度代谢网络模型的代谢工程设计预测方法
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GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
AU2015279057B2 (en) 2014-06-27 2019-02-28 Dsm Ip Assets B.V. A method for improving the nutritional value of animal feed
BR112018067862A2 (pt) * 2016-03-08 2019-04-16 Basf Enzymes Llc método para melhorar a eficiência de produção de etanol por levedura.
US10351832B2 (en) 2016-06-30 2019-07-16 Fornia Biosolutions, Inc. Phytases and uses thereof
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US9528096B1 (en) * 2016-06-30 2016-12-27 Fornia Biosolutions, Inc. Phytases and uses thereof
CN109536399B (zh) * 2018-10-16 2021-05-14 北京泛球生物科技有限公司 一种维吉尼亚霉素高产菌株的高通量筛选方法
WO2021239206A1 (en) * 2020-05-25 2021-12-02 N.V. Nutricia Process for lowering phytic acid in cereals
CN112979356A (zh) * 2021-05-24 2021-06-18 中国农业科学院农业环境与可持续发展研究所 微生物基因丰度及胞外酶活性在秸秆还田固碳中的应用

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US5593963A (en) 1990-09-21 1997-01-14 Mogen International Expression of phytase in plants
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US6183740B1 (en) * 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
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Patent Citations (1)

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Publication number Publication date
IN2004DE03129A (en) 2005-04-01
CN1592791A (zh) 2005-03-09
WO2002095003A2 (en) 2002-11-28
BR0208851A (pt) 2006-02-07
EP1478769A2 (en) 2004-11-24
CA2441666A1 (en) 2002-11-28
EP1478769A4 (en) 2006-07-19
BRPI0208851B1 (pt) 2015-07-21
KR20040099097A (ko) 2004-11-26
JP2005505254A (ja) 2005-02-24
WO2002095003A3 (en) 2004-09-23
BR122014019327B1 (pt) 2015-12-15
US6855365B2 (en) 2005-02-15
US20020136754A1 (en) 2002-09-26

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