CN1587961A - Collodion investment and use in fault anatomy research - Google Patents
Collodion investment and use in fault anatomy research Download PDFInfo
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- CN1587961A CN1587961A CN 200410072234 CN200410072234A CN1587961A CN 1587961 A CN1587961 A CN 1587961A CN 200410072234 CN200410072234 CN 200410072234 CN 200410072234 A CN200410072234 A CN 200410072234A CN 1587961 A CN1587961 A CN 1587961A
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Abstract
The invention relates to a method of colloxylin embedding used insurgery and its application in fault anatomy research, which includes the steps that (1) choose specimen anywhere of corpse fixation by formaldehyde; (2) specimen decalcification : use 10% hydrochloric acid to soak specimen fifteen to thirty days; (3) combine colloxylin embedding method with L-type microtome to prepare continuous fault section. Its effect is that compared with common tissue slice, the fault section has continuity and no loss, whose thickness can be chosen willingly during 25mum to 30mum match magnetic resonance image, and it can show clearly any adjoining structure of fault dissection and reflect local fault anatomy relationship of significant clinical meaning. Being five to ten times larger than common fault section observation range and one twentieth to one tenth of thickness, the manufacture of fault section has optimized preservation method of colloxylin section.
Description
Technical field
The present invention relates to surgery and adopts a kind of method of celloidin embedding and the application in sectional anatomy thereof.
Background technology
Collodion (Colloidion, Nitrocellulose or Soluble gun cotton) investment technology be a kind of to single organization or organ (as: nerve fiber or heart) sample fix, dewater, soak into, the method for embedding, sclerosis, clamp dog and section; Be only limited to histology, the observation in 3 * 3mm or 5 * 5mm visual field under the light microscopic.The organization embedding block is long-pending generally thick at 2 * 3 * 3mm or 3 * 5 * 5mm, can not reflect the local sectional anatomy relation that clinical meaning is great; As: the neurovascular cloth relation of joining, the sectional anatomy relation of joining cloth of nerve, blood vessel and internal organs.
Sectional anatomy (Sectional anatomy) is the science with tomography method research and expression human body normal morphology structure and basic function thereof.The research method of at present domestic sectional anatomy has:
1, freezing microtome section technology (Cryotomy) is the conventional method of human body tomography sample preparations, and its basic step has:
1. draw materials the integral body of corpse or local;
2. fixing, with 10% formaldehyde fixed more than 6 months;
3. graticule;
4. freezing;
5. section is made the tomography sample with carpenter's saw or band-sawing machine, and also available large freezing microtome or milling machine carry out milling, or with other tool makings such as planes.
2, biological plasticized technology (Plastination), be the good liquid macroimolecule poly-compounds of selecting some permeance property for use be monomer as plasticiser, the curing of getting together of the intracellular moisture of replacement tissue, whole plasticizing process comprises substantially:
1. fixing, fix with the formaldehyde arterial perfusion;
2. dehydration is dewatering agent usually with acetone;
3. vacuum impregnation is under negative pressure or vacuum condition, with the acetone of plasticiser replacement tissue;
4. cure process;
5. plasticizing section is made the tomography sample with scroll saw.
The problem that the above method of the research of sectional anatomy exists is as follows:
1. freezing sawing, slice thickness are generally at 1-2cm, and along with the development of clinical magnetic resonance image, scanning slice is more and more thinner, the sectional slice trend slimming that matches.Can not satisfy the development need of iconography and surgery, can not provide full and accurate for the tomography structure of 1-2cm thickness, particularly mate the sectional slice data and the difficult operative approach data of magnetic resonance image (MRI); The magnetic resonance image (MRI) scanning slice is generally 1.0-3.0mm.
2. freezing milling can not keep the sample that milling is fallen, can only to substrate take pictures or as calculated machine handle and to obtain simulation data.
3. biological plasticized scroll saw, sectional slice thickness are 1.2mm, and the swage set loss is more than 0.3mm; The continuity that can not truly reflect section.The requirement of plastifying technology appointed condition is high, expense is expensive, and is at present domestic universal as yet.
Summary of the invention
The objective of the invention is to remedy weak point of the prior art, and provide a kind of
Can take following technical scheme to realize purpose.
The celloidin embedding method, the investment step:
1. the sample of drawing materials: any position of corpse after formaldehyde fixed;
2. sample decalcification: the sample of drawing materials that will design can be with till the acupuncture people until bone tissue with 10% salt acid soak 15-30 days;
3. the celloidin embedding method is produced L type microtome with Germany and combine the continuous sectional slice of preparation.
Prior art has following advantage and effect relatively:
Realize that its key of the application of celloidin embedding law technology in sectional anatomy is the method for thinking and the technological innovation of science: celloidin embedding method, the decalcification of cross sectional anatomy piece of tissue and Germany are produced infusing drugs in wine L type microtome (JUNG AG.) integrate the section of preparation cross sectional anatomy.
1, is different from general histotomy, particularly is different from present domestic cross sectional anatomy section.
2, the cross sectional anatomy section of present technique development has continuity, does not have the saw consumption, can select the thin and thick coupling magnetic resonance image (MRI) (MRI) of cutting into slices arbitrarily; Section thin and thick range of choice is at 25 μ m-3.0mm, and general magnetic resonance image (MRI) scanning slice is at 1.0-3.0mm.
3, any adjacent structure of the clear displaying cross sectional anatomy of cross sectional anatomy section energy of present technique development:, can be able to reflect the local sectional anatomy relation that clinical meaning is great as cranium brain, eye socket or visual organ, spinal area, extremities joint, pelvic part etc. and clinical labyrinth in close relations and neighbouring relationship thereof; As: the neurovascular cloth relation of joining, the sectional anatomy relation of joining cloth of nerve, blood vessel and internal organs.
4, the cross sectional anatomy sections observation scope of technology development is than the big 5-10 of general tissue times, than the little 10-20 of general cross sectional anatomy slice thickness doubly.
5, optimized the store method (referring to the store method of aforementioned collodion cross sectional anatomy section) of cellodion section.
Embodiment
Embodiment 1, celloidin embedding method, the investment step:
1. the sample of drawing materials: any position of corpse after formaldehyde fixed, as head, spinal area, pelvic part, extremities joint etc.; 2. sample decalcification: the sample of drawing materials that will design can be with till the acupuncture people until bone tissue with 10% salt acid soak 15-30 days; 3. the celloidin embedding method is produced L type microtome with Germany and combine the continuous sectional slice of preparation.
Embodiment 2, celloidin embedding method, on embodiment 1 basis, the volume range of drawing materials is in that (50 * 50 * 50mm) to (180 * 180 * 150mm), slice thickness is arranged on (0.5-1.0mm), or is arranged on (25-100 μ m).
Embodiment 3, the application of celloidin embedding method in sectional anatomy, on embodiment 1 or 2 bases, preparation of cross sectional anatomy piece of tissue and embedding step
1. cross sectional anatomy piece of tissue decalcification;
2. flushing: after the taking-up of decalcification piece of tissue, use circulating water flushing 24 hours;
3. conventional dehydration;
4. impregnation: the piece of tissue after will dewatering immerses in 5% collodion earlier, and the pressure with 66kPa in vacuum tank vacuumized 30 minutes, leave standstill 1 day after, vacuumize again, leave standstill again and immerse in 8% collodion after 1 day; Vacuumize 2 times with similarity condition.
5. embedding and clamp dog: with 15% collodion as embedding medium, the about 15-30 of the formation of embedded block days (this time depends primarily on the velocity of evaporation of collodion solvent).Embedded block is put sclerosis preservation in people's 70% alcohol.
Embodiment 4, the application of celloidin embedding method in sectional anatomy, on embodiment 3 bases, the preparation method of cross sectional anatomy section:
Above-mentioned celloidin embedding block welding is produced to Germany on the weldering pallet of infusing drugs in wine L type microtome, and weldering pallet maximum gauge is (200mm), and weldering holding tray surface to slicer distance is (180mm); The volume range of drawing materials is set in that (50 * 50 * 50mm) to (180 * 180 * 150mm), section displaying scope is also in that (50 * 50 * 50mm) to (in 180 * 180 * 150mm).
Application example:
3 routine spinal area (spinal nerve root in waist interverbebral disc, intervertebral disc joint, the canalis spinalis) had been carried out experimental study to example to 20 routine basis cranii saddle districts (cavernous sinus and cranial nerve thereof, internal carotid and sphenoid sinus) in 1,1998 to 2000.Published this technical method paper " application of celloidin embedding method on preparation sectional anatomy sample " (, rolling up the 91st page of the 1st phase in 2000 the 18th) in 2000 first at home referring to Chinese clinical anatomy magazine.
Example was finished 9 routine corpse brain collodion cross sectional anatomy serial section in 2,2000 to 2003, and cross sectional anatomy is combined with magnetic resonance image (MRI) (MRI), had a learned dissertation published 2 pieces.
1. " sectional anatomy of medial temporal lobe structure " (referring to Chinese fault image anatomy magazine, 2000 the 4th volume the 1st phase 9-12 pages or leaves);
2. " cross sectional anatomy of medial temporal lobe structure and the comparative study of MRI " (referring to Chinese clinical anatomy magazine, 2003 the 21st volume the 1st phase 30-32 pages or leaves.
Example was finished adult head and neck vein porose area 7 examples, eye socket (visual organ) 50 examples, knee joint 8 routine collodion cross sectional anatomy serial section researchs in 3,2002 so far.It is as follows to have a learned dissertation published:
1. " cross sectional anatomy of outside portion structure and clinical meaning behind the knee ", anatomy magazine, 2003 the 26th volume the 5th phase 480-483 pages or leaves.
2. " cross sectional anatomy in flesh awl gap ", anatomy magazine, 2003 the 26th volume the 4th phase 381-385 pages or leaves.
3. the thin layer cross sectional anatomy of the Jugular Foramen " research " Chinese clinical anatomy magazine, 2003 the 21st volume the 5th phase 451-453 pages or leaves.
4. " sectional anatomy of optic nerve and clinical meaning thereof ", Chinese clinical anatomy magazine, 2003 the 21st volume the 5th phase 454-456 pages or leaves.
5. " knee joint transversal section dissect and clinical meaning ", symposial when the 3rd whole nation dissected with clinical medicine, dissect and the clinical journal article 34-36 page or leaf that collects in Mount Huang.
6. " at the bottom of the internal auditory meatus cross sectional anatomy research ", symposial when the 3rd whole nation dissected with clinical medicine, dissect and the clinical journal article 36-37 page or leaf that collects in Mount Huang.
Symposial when 7. " store method that the cellodion section sample is box-packed, " the 3rd whole nation is dissected with clinical medicine, dissect and the clinical journal article 265-266 page or leaf that collects in Mount Huang.
Example 4, Chinese famous anatomist, the vice-president Liu Shuwei of medical college of Shandong University professor, Li Yun gave birth to such evaluation is arranged in doctoral advisor's recommendation on July 18th, 2004: " the Li Yunsheng professor writes many books in the clinical anatomy field; formed the research characteristic of oneself; especially take the course of its own aspect the fault image anatomical research utilizing the cellodion section technology to carry out ... " (referring to copy the material).
Claims (4)
1, a kind of celloidin embedding method is characterized in that the investment step:
1. the sample of drawing materials: any position of corpse after formaldehyde fixed;
2. sample decalcification: the sample of drawing materials that will design can be with till being needled into until bone tissue with 10% salt acid soak (15-30) day;
3. the celloidin embedding method is produced L type microtome with Germany and combine the continuous sectional slice of preparation.
2, celloidin embedding method according to claim 1 is characterized in that: the volume range of drawing materials is in that (50 * 50 * 50mm to 180 * 180 * 150mm), slice thickness are arranged on (0.5-1.0mm), or are arranged on (25-100 μ m).
3, the application of celloidin embedding method according to claim 1 and 2 in sectional anatomy is characterized in that preparation of cross sectional anatomy piece of tissue and embedding step:
1. cross sectional anatomy piece of tissue decalcification;
2. flushing: after the taking-up of decalcification piece of tissue, use circulating water flushing 24 hours;
3. conventional dehydration;
4. impregnation: the piece of tissue after will dewatering immerses in (5%) collodion earlier, and the pressure with (66kPa) in vacuum tank vacuumized 30 minutes, leave standstill 1 day after, vacuumize again, leave standstill again and immerse in (8%) collodion after 1 day; Vacuumize 2 times with similarity condition;
5. embedding and clamp dog: as embedding medium, embedded block inserted in (70%) alcohol sclerosis and preserving with 15% collodion.
4, the application of celloidin embedding method according to claim 3 in sectional anatomy is characterized in that the preparation method that cross sectional anatomy is cut into slices:
Above-mentioned celloidin embedding block welding is produced to Germany on the weldering pallet of infusing drugs in wine L type microtome, and weldering pallet maximum gauge is (200mm), and weldering holding tray surface to cutting knife distance is (180mm); The volume range of drawing materials is set in that (50 * 50 * 50mm) to (180 * 180 * 150mm), section displaying scope is also in that (50 * 50 * 50mm) to (in 180 * 180 * 150mm).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410072234 CN1587961A (en) | 2004-09-29 | 2004-09-29 | Collodion investment and use in fault anatomy research |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410072234 CN1587961A (en) | 2004-09-29 | 2004-09-29 | Collodion investment and use in fault anatomy research |
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| CN1587961A true CN1587961A (en) | 2005-03-02 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101739880A (en) * | 2009-11-19 | 2010-06-16 | 裴秋艳 | Pretreatment method of heart of foetus |
| CN102580695A (en) * | 2012-02-17 | 2012-07-18 | 温州大学 | Method for adsorbing copper ions in water by using gamma-polyglutamic acid and pyroxylin formed film |
| CN102037342B (en) * | 2008-05-19 | 2013-03-13 | 樱花精机株式会社 | Tissue piece treating apparatus |
| CN103050043A (en) * | 2013-01-25 | 2013-04-17 | 胡光强 | Method for manufacturing imitative sectional imaging specimen |
| CN103975229A (en) * | 2011-09-29 | 2014-08-06 | 迈阿密大学 | Ultra-rapid diagnostic tissue preparation as an alternative to frozen section |
-
2004
- 2004-09-29 CN CN 200410072234 patent/CN1587961A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102037342B (en) * | 2008-05-19 | 2013-03-13 | 樱花精机株式会社 | Tissue piece treating apparatus |
| CN101739880A (en) * | 2009-11-19 | 2010-06-16 | 裴秋艳 | Pretreatment method of heart of foetus |
| CN101739880B (en) * | 2009-11-19 | 2015-01-14 | 裴秋艳 | Pretreatment method of heart of foetus |
| CN103975229A (en) * | 2011-09-29 | 2014-08-06 | 迈阿密大学 | Ultra-rapid diagnostic tissue preparation as an alternative to frozen section |
| CN103975229B (en) * | 2011-09-29 | 2017-09-22 | 迈阿密大学 | It is used as the ultrafast diagnosis tissue preparation of the substitute of frozen section |
| CN102580695A (en) * | 2012-02-17 | 2012-07-18 | 温州大学 | Method for adsorbing copper ions in water by using gamma-polyglutamic acid and pyroxylin formed film |
| CN103050043A (en) * | 2013-01-25 | 2013-04-17 | 胡光强 | Method for manufacturing imitative sectional imaging specimen |
| CN103050043B (en) * | 2013-01-25 | 2015-07-22 | 胡光强 | Method for manufacturing imitative sectional imaging specimen |
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