CN101739880B - Pretreatment method of heart of foetus - Google Patents
Pretreatment method of heart of foetus Download PDFInfo
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- CN101739880B CN101739880B CN200910226304.9A CN200910226304A CN101739880B CN 101739880 B CN101739880 B CN 101739880B CN 200910226304 A CN200910226304 A CN 200910226304A CN 101739880 B CN101739880 B CN 101739880B
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- 210000002216 heart Anatomy 0.000 title claims abstract description 46
- 238000002203 pretreatment Methods 0.000 title claims abstract description 12
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 72
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000001605 fetal effect Effects 0.000 claims description 29
- 210000003754 fetus Anatomy 0.000 claims description 28
- 230000033764 rhythmic process Effects 0.000 claims description 21
- 230000008014 freezing Effects 0.000 claims description 11
- 238000007710 freezing Methods 0.000 claims description 11
- 238000003325 tomography Methods 0.000 claims description 11
- 239000008098 formaldehyde solution Substances 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000002592 echocardiography Methods 0.000 description 8
- 230000000747 cardiac effect Effects 0.000 description 6
- 239000000523 sample Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 3
- 210000003462 vein Anatomy 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004351 coronary vessel Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003801 milling Methods 0.000 description 2
- 238000003793 prenatal diagnosis Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 208000002330 Congenital Heart Defects Diseases 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000003698 chordae tendineae Anatomy 0.000 description 1
- 208000028831 congenital heart disease Diseases 0.000 description 1
- 210000003748 coronary sinus Anatomy 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000003709 heart valve Anatomy 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003540 papillary muscle Anatomy 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 210000003516 pericardium Anatomy 0.000 description 1
- 210000003492 pulmonary vein Anatomy 0.000 description 1
- 210000001562 sternum Anatomy 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
Abstract
The invention discloses a pretreatment method of the heart of foetus. The invention is realized by using the following technical scheme: the pretreatment method of the heart of foetus is provided; and the method is characterized by comprising the following steps: 1. soaking the heart of foetus for four weeks in a container with 4% of formaldehyde solution; 2. placing the soaked heart of foetus in a -20 DEG C of refrigerator to freeze for 12h; and 3. taking the heart of foetus out of the refrigerator, and performing continuous frozen section to obtain 60mu m of slices while performing macro shooting with a digital camera to continuously obtain data on section of the heart of foetus. The method of the invention is novel, the operation method is easy to handle and the cost is cheap, thus the establishment of a three-dimensional model of the heart of foetus can be possible.
Description
Technical field: the invention belongs to medical pathologies histological techniques field, particularly a kind of pretreatment method of heart of foetus.
Background technology: congenital heart disease accounts for life birth baby's 4.05 ‰-12.3 ‰, being current modal congenital abnormality, is also the first cause causing Infant and child deaths.Prenatal diagnosis of fetal CMH can reduce the birth rate of serious complicated CMH, improves the life quality of viable fetus.Current Study of Fetal Echocardiography inspection is the only method of pre-natal diagnosis CHD, but is subject to the impact of fetus position and specific cycle thereof, compares with adult's echocardiography, and Study of Fetal Echocardiography technical difficulty is comparatively large, up to the present non-popularization and application.Set up heart of fetus anatomical atlas and heart of fetus three-dimensional model, be beneficial to beginner and understand heart of fetus tomography and three-D space structure, grasp Study of Fetal Echocardiography technology, also can be used for the research of heart of fetus function and CMH surgical field.Since the nineties, along with raising that is ultrasonic, MRI and CT resolution, in succession establish human adult heart image faultage image data storehouse, and build cardiac three-dimensional model based on this, be applied to teaching, scientific research, Diagnosis and Treat field.Along with the foundation of Visible Human Dataset, be established by the three-dimensional model that human adult heart is dissected based on section, further increase picture quality.But up to the present, there is not the report of heart of fetus three-dimensional model.
Cardiac tomogram anatomical atlas in the past and three-dimensional reconstruction data come from " Visible man " (Visible Human Proiect of the U.S. respectively, VHP) and China (Virtual Chinese Male, VCH) database, it is the continuous crosscut to human body integral sample, heterogeneity and the acquisition of large volume to tomography of sample material bring larger difficulty, defining instrument and equipment and tomography thickness, is therefore select milling machine to carry out the continuous crosscut of the thick 0.2-1.0mm of tomography to the total preparation after freezing.And heart of fetus structure is trickle, thin tomography is the prerequisite of complete display cardiac structure.The present invention is using heart of fetus and partly other thoracic organs are as cutting object, and removal breastbone, backbone and rib, decrease heterogeneity and the cutting volume of sample, use freezing microtome to carry out thickness 60um serial section, effectively reduce cutting thickness.VHP does not do process in early stage to cardiovascular system, VCH fills with the red filling agent of people in arterial system, be convenient to form a sharp contrast with vein, but all special process in early stage is not carried out to heart, adopt direct freezing method to the crosscut of total preparation milling machine, cardiac tomogram tangent plane is clear shows chamber chamber, valve, trunk and coronary vasculature, therefore we once attempted directly carrying out thin layer frozen section to the heart lung preparation after freezing, but at the freezing tangent plane of image data, chamber cavity configuration is unclear, locular wall and surrounding tissue are demarcated unclear, consider relevant with fetal tissue feature.Therefore a kind of pretreatment method of heart of foetus is invented.
Summary of the invention: the object of the invention is the deficiency for overcoming existing for prior art, and invent a kind of pretreatment method of heart of foetus.
The present invention is realized by following technical scheme:
Invent a kind of pretreatment method of heart of foetus, it is characterized in that: its method comprises the following steps:
1, fetal rhythm is soaked in the formalin of in container 4%, soak time surrounding.
2, in the refrigerator that the fetal rhythm after being soaked by 4% formaldehyde is positioned over-20 DEG C freezing 12 hours.
3, after the fetal rhythm after being soaked by 4% formaldehyde takes out in refrigerator, carry out the continuous frozen section of thickness 60um, use digital camera microshot simultaneously, obtain heart of fetus tomography tangent plane data continuously.
Advantage of the present invention and beneficial effect:
1, after pre-service being carried out to heart of fetus with formaldehyde fixation, show chamber chamber, trunk and valve structure image data tangent plane is not only clear, simultaneously clearly show coronary sinus vein, main coronary artery and branch thereof, heart and surrounding tissue boundary clearly, therefore think that formaldehyde fixation is desirable pretreatment method of heart of foetus.
2, by being analyzed the heart of fetus frozen section of different pretreatments, formaldehyde fix after sample tangent plane cardiac structure clear, not only clearly show chamber chamber, cardiac valves, chordae tendineae, papillary muscle, pulmonary vein, simultaneously clear show ultrasonic under be not easy the cardiac structure that shows or do not show, as coronary artery master props up and branch, coronary vein and pericardium.Can be used for setting up heart of fetus anatomical atlas and three-dimensional model.
3, the inventive method is novel, easily grasps method of operating, low cost, and making to establish heart of fetus three-dimensional model has had possibility.
Embodiment:
Be described further the present invention further below by embodiment, following examples are descriptive is not determinate, can not limit protection scope of the present invention with this.
A kind of pretreatment method of heart of foetus, its step comprises:
1, fetal rhythm is soaked in the formalin of in container 4%, soak time surrounding, without the need to stirring in soak period, without the need to changing formalin.Formaldehyde involved in the present invention is by commodity formaldehyde formulations, and concentration is 4%.
2, in the refrigerator that the fetal rhythm after being soaked by 4% formaldehyde is positioned over-20 DEG C freezing 12 hours, the refrigerator short time can be opened between ice period and observe fetal rhythm, be no more than one minute observing time.
3, the sagittal sections of thickness 60um, crown and horizontal frozen section is carried out respectively after the fetal rhythm after being soaked by 4% formaldehyde takes out in refrigerator, use digital camera microshot simultaneously, continuous acquisition heart of fetus tomography tangent plane data, obtained tangent plane and Study of Fetal Echocardiography tangent plane used are compared analysis, to build heart of fetus three-dimensional model and heart of fetus anatomical atlas.
Embodiment 1
(1), the fetal rhythm of 25 weeks is soaked in the formalin of 4%, soak time surrounding, without the need to stirring in soak period, without the need to changing formalin.
(2) in the refrigerator that the fetal rhythm after being soaked by 4% formaldehyde is positioned over-20 DEG C freezing 12 hours.
(3) the sagittal sections section of thickness 60um is carried out after the fetal rhythm after being soaked by 4% formaldehyde takes out in refrigerator, use digital camera microshot simultaneously, continuous acquisition heart of fetus tomography tangent plane data, obtained tangent plane and Study of Fetal Echocardiography tangent plane used are compared analysis, to build heart of fetus three-dimensional model and heart of fetus anatomical atlas.
Embodiment 2
(1), the fetal rhythm fetal rhythm of 28 weeks is soaked in the formalin of 4%, soak time surrounding, without the need to stirring in soak period, without the need to changing formalin.
(2) in the refrigerator that the fetal rhythm after being soaked by 4% formaldehyde is positioned over-20 DEG C freezing 12 hours.
(3) the continuous coronal frozen section of thickness 60um is carried out after the fetal rhythm after being soaked by 4% formaldehyde takes out in refrigerator, use digital camera microshot simultaneously, continuous acquisition heart of fetus tomography tangent plane data, obtained tangent plane and Study of Fetal Echocardiography tangent plane used are compared analysis, to build heart of fetus three-dimensional model and heart of fetus anatomical atlas.
Embodiment 3
(1), the fetal rhythm fetal rhythm of 30 weeks is soaked in the formalin of in container 4%, soak time surrounding, without the need to stirring in soak period, without the need to changing formalin.
(2) in the refrigerator that the fetal rhythm after being soaked by 4% formaldehyde is positioned over-20 DEG C freezing 12 hours.
(3) the continuous transverse frozen section of thickness 60um is carried out after the fetal rhythm after being soaked by 4% formaldehyde takes out in refrigerator, use digital camera microshot simultaneously, continuous acquisition heart of fetus tomography tangent plane data, obtained tangent plane and Study of Fetal Echocardiography tangent plane used are compared analysis, to build heart of fetus three-dimensional model and heart of fetus anatomical atlas.
Claims (1)
1. a pretreatment method of heart of foetus, is characterized in that: its method comprises the following steps:
(1) fetal rhythm is soaked in the formalin of in container 4%, soak time surrounding;
(2) in the refrigerator that the fetal rhythm after being soaked by 4% formaldehyde is positioned over-20 DEG C freezing 12 hours;
(3) after the fetal rhythm after being soaked by 4% formaldehyde takes out in refrigerator, carry out the continuous frozen section of thickness 60um, use digital camera microshot simultaneously, obtain heart of fetus tomography tangent plane data continuously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910226304.9A CN101739880B (en) | 2009-11-19 | 2009-11-19 | Pretreatment method of heart of foetus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910226304.9A CN101739880B (en) | 2009-11-19 | 2009-11-19 | Pretreatment method of heart of foetus |
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| Publication Number | Publication Date |
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| CN101739880A CN101739880A (en) | 2010-06-16 |
| CN101739880B true CN101739880B (en) | 2015-01-14 |
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| CN200910226304.9A Expired - Fee Related CN101739880B (en) | 2009-11-19 | 2009-11-19 | Pretreatment method of heart of foetus |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018195874A1 (en) * | 2017-04-27 | 2018-11-01 | 深圳迈瑞生物医疗电子股份有限公司 | Ultrasonic detection method and ultrasonic imaging system for fetal heart |
| CN108182725A (en) * | 2017-12-20 | 2018-06-19 | 中国人民解放军陆军军医大学第二附属医院 | A kind of method for establishing digitlization Single Cardiac Ventricle heart of fetus structured teaching model |
| CN111929095A (en) * | 2020-07-10 | 2020-11-13 | 南京农业大学 | Small sample frozen section rapid imaging method |
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|---|---|---|---|---|
| CN2607635Y (en) * | 2002-12-20 | 2004-03-24 | 张导华 | Human heart model |
| CN1587961A (en) * | 2004-09-29 | 2005-03-02 | 李云生 | Collodion investment and use in fault anatomy research |
| CN1588489A (en) * | 2004-08-31 | 2005-03-02 | 吕滨 | Coronary artery stenosis model and its producing method |
| CN101779622A (en) * | 2009-11-25 | 2010-07-21 | 中国人民解放军第三军医大学第二附属医院 | Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease |
| CN101779621A (en) * | 2009-11-25 | 2010-07-21 | 中国人民解放军第三军医大学第二附属医院 | Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease |
| CN101810159A (en) * | 2009-11-25 | 2010-08-25 | 中国人民解放军第三军医大学第二附属医院 | Method for preparing fetal congenital heart disease aortic arch long-axis section teaching specimen |
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| US7813785B2 (en) * | 2003-07-01 | 2010-10-12 | General Electric Company | Cardiac imaging system and method for planning minimally invasive direct coronary artery bypass surgery |
| CN101379960A (en) * | 2008-09-30 | 2009-03-11 | 太原钢铁(集团)有限公司 | Heart left ventricle ventricular aneurysm model and production method thereof |
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2009
- 2009-11-19 CN CN200910226304.9A patent/CN101739880B/en not_active Expired - Fee Related
Patent Citations (6)
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|---|---|---|---|---|
| CN2607635Y (en) * | 2002-12-20 | 2004-03-24 | 张导华 | Human heart model |
| CN1588489A (en) * | 2004-08-31 | 2005-03-02 | 吕滨 | Coronary artery stenosis model and its producing method |
| CN1587961A (en) * | 2004-09-29 | 2005-03-02 | 李云生 | Collodion investment and use in fault anatomy research |
| CN101779622A (en) * | 2009-11-25 | 2010-07-21 | 中国人民解放军第三军医大学第二附属医院 | Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease |
| CN101779621A (en) * | 2009-11-25 | 2010-07-21 | 中国人民解放军第三军医大学第二附属医院 | Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease |
| CN101810159A (en) * | 2009-11-25 | 2010-08-25 | 中国人民解放军第三军医大学第二附属医院 | Method for preparing fetal congenital heart disease aortic arch long-axis section teaching specimen |
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| CN101739880A (en) | 2010-06-16 |
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Owner name: PEOPLE'S HOSPITAL OF BEIJING UNIV. Free format text: FORMER OWNER: PEI QIUYAN Effective date: 20150128 |
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Effective date of registration: 20150128 Address after: 100044 Beijing city Xicheng District Xizhimen Street No. 11 Patentee after: PEKING UNIVERSITY PEOPLE'S Hospital Address before: 100044 Department of Obstetrics and Gynecology, Beijing people's Hospital, 11 South Avenue, Beijing, Xicheng District, Xizhimen Patentee before: Pei Qiuyan |
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