CN101779622B - Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease - Google Patents
Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease Download PDFInfo
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- CN101779622B CN101779622B CN2009101915721A CN200910191572A CN101779622B CN 101779622 B CN101779622 B CN 101779622B CN 2009101915721 A CN2009101915721 A CN 2009101915721A CN 200910191572 A CN200910191572 A CN 200910191572A CN 101779622 B CN101779622 B CN 101779622B
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Abstract
The invention relates to a method for preparing a teaching specimen of a left ventricular outflow tract view of a stillbirth suffered from a congenital heart disease. In the method, a free heart specimen is prepared after a selected artificially-labored fetus having a malformed heart is antiseptically treated; and in the left ventricular outflow tract view, the horizontal axis of the view is parallel to the long axis of the heart by taking a pulmonary conus as a start point of a cross section, the cross section is up to a superior vena cava through aorta ascendens and down to the apex of the heart through an anterior interventricular groove. The obtained apical four-chamber view specimen has the advantages of integrated and complete structures of all parts, no damage to microstructures in the heart, the same shape as that in a real living body, and capability of meeting the requirements of medical teaching and practice and the like, and is suitable for schools, hospitals or medical research institutes.
Description
Technical field
The present invention relates to the preparation method of teaching specimen, be specifically related to a kind of left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen.
Background technology
It is reported; Children with congenital heart disease accounts for 8%～12% of China's natus; Mean that there is the birth of 120,000～200,000 children with congenital heart disease every year in China, wherein complicated, at present treatment means still can not reach good result of treatment or the early stage dead children with congenital heart disease in birth back accounts for 20%, be the neonate and the childhood one of major causes of death; Brought heavy spirit and financial burden for family and society, bring endless misery to infant.Ultrasonic examination almost is the unique selection of the antenatal diagnostic imaging of heart of fetus deformity.Therefore, carrying out heart of fetus ultrasonic examination is the important component part of antenatal ultrasonic monitoring prevision foetus health, and it meets improving the overall quality of newborns of China, reduces the prenatal and postnatal care state basic policy of low age death rate.
The heart of fetus deformity is divided into lethal deformity and non-lethality deformity; Lethal heart malformations complexity is high, so heart of fetus ultrasonic examination is a technology that training property is stronger; Content is complicated, has comprised rudimentary knowledge such as human dissection, pathological anatomy, embryo growth and development and pathologic, physiologic.
Though most of ultrasonic doctors have ultrasound medicine basis and Ultrasonic Diagnosis experience for many years preferably now; But the basic theory of relevant heart of fetus Ultrasonic Diagnosis is weak; Standardization tangent plane and anatomical structure to heart of fetus lack understanding; And blindly inspection indeterminate to the ultrasonographic content of heart of fetus; The heart special construction that can reflect ventricular septal defect, double outlet of right ventricle congenital heart disease on the left ventricular outflow tract view tangent plane, so the left ventricular outflow tract view of fetus suffered from congenital heart disease teaching specimen can reflect the heart special construction of ventricular septal defect, double outlet of right ventricle congenital heart disease intuitively, for teaching research provides reference.
Summary of the invention
The purpose of this invention is to provide the left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen; Utilize this method can produce the left ventricular outflow tract view tangent plane sample of the heart of fetus of suffering from congenital heart disease, each bilge construction is complete complete, does not damage the inner micro-structure of heart; The special construction that can reflect congenital heart disease on the left ventricular outflow tract view tangent plane intuitively; And sample has the light cleaning, characteristics with long preservation period, and being used to impart knowledge to students to reach the effect of intuitivism apprehension.
For achieving the above object, technical scheme proposed by the invention is: the left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen may further comprise the steps:
1) the selection of material is the induced labor stillborn foetus of heart development deformity, and prepares impregnating fluid;
2) umbilical cord is repaiied cut neatly, comprise a bigger umbilical vein and two less arteria umbilicalis tangent planes on the umbilical cord cross section, umbilical vein catheterization perfusion impregnating fluid;
3) after sample successfully pours into, place week age, treat that impregnating fluid is penetrated into each one of whole body;
4) make free heart lung preparation, connective tissue is divested totally, flowing water flushing, dried for standby;
5) make the left ventricular outflow tract view of fetus suffered from congenital heart disease sample, concrete operations are following: begin from apex, make the longitudinal axis of section parallel with long axis of heart, the right and left bit parallel of the transverse axis of section and heart; 1～2cm place, front upper place of edge along the heart left and right sides, bottom direction is entad broken off heart near the pulmonary vein inlet of the middle part of atrium dextrum and atrium sinistrum;
6) treat that the tangent plane sample is air-dry after, to the colouring of the emphasis anatomical structure that shown, treat to smear animal glue after the pigment drying, use transparent box body fixed preparation, encapsulation again, inject 5% formalin solution at last and seal preservation.
Adopt the technique effect that technique scheme reached to be: resulting each bilge construction of left ventricular outflow tract view tangent plane sample is complete complete; Do not damage the inner micro-structure of heart, with truly in vivo shape is identical, consistent with ultrasonic examination standard tangent plane structure; The sample light is clean; But long preservation, cardiac ultrasonic dissec fabrication cycle is short, and is superior in quality.Problems such as present fetal ultrasound cardiogram teaching is abstract, thereby the complicacy teaching efficiency is bad, influence application that it has solved; It is easy to have method, and Production Time is short, and transparence is effective; Be well positioned to meet the advantage that medical teaching and practice etc. need, be suitable for school, hospital or medical research mechanism.
Further, the emphasis anatomical structure is damaged interventricular septum structure in the described step 6).Its structure that shows abnormality is carried out red-label, can produce the congenital heart disease left ventricular outflow tract view tangent plane teaching specimen of ventricular septal defect.
Further, the emphasis anatomical structure is two a main arteries origin and out of shape in the described step 6).Carry out red-label and can produce double outlet of right ventricle congenital heart disease left ventricular outflow tract view tangent plane teaching specimen.
Embodiment
We can know by anatomical knowledge, and atrium dextrum, chamber are positioned at the right front on room, interventricular septum plane, and right ventricle is the chambers of the heart of forefront, and the atrium dextrum is the chambers of the heart the most on the right side, constitute heart right border; Atrium sinistrum, chamber are positioned at the left back on room, interventricular septum plane, and the atrium sinistrum is the chambers of the heart at rear, and left ventricle is the chambers of the heart of side of keeping left most, constitutes left heart edge.The primary section of cardiac structure is three mutual rectangular sections along its major axis and minor axis, the i.e. section of sagittal section of the horizontal section of long axis of heart, long axis of heart (anteroposterior position section) and heart minor axis.The major axis of heart is the line between apex and the heart bottom central; The minor axis of heart is another axis that at right angles intersects with its major axis.Cut heart according to each standard tangent plane of cardiac ultrasonic inspection respectively, the knife edge is sharp keen when making tangent plane, guarantees neat in edge; Disposable success.The concrete operations step is following:
The first step, the selection of material are the induced labor stillborn foetus of heart development deformity, and prepare 10% formalin solution as impregnating fluid.
Second goes on foot, and umbilical cord is repaiied cut neatly, comprises a bigger umbilical vein and two less arteria umbilicalis tangent planes on the umbilical cord cross section, adopts the well-bucket perfusion to carry out the umbilical vein catheterization perfusion.
In the 3rd step, after sample successfully pours into (under the unblocked situation of umbilical vein, get final product in general 3 hours), approximately pass through the week age impregnating fluid and may penetrate into the whole body each several part; In order to next step operation.
The 4th step, produce free heart lung preparation (prop up about the descending aorta of reservation certain-length and pulmonary artery, show) with convenient observation and structure, connective tissue is divested totally, flowing water washes, dried for standby.
The 5th step; Make the left ventricular outflow tract view of fetus suffered from congenital heart disease sample; Concrete operations are following: the left ventricular outflow tract view tangent plane of heart is with the starting point of pulmonary conus as section; Make the axis of the transverse axis and the heart of tangent plane, upwards through aorta ascendens to superior vena cava, make a tangent plane through the through apex of anterior interventricular groove downwards.Sustainer origin and traveling, interventricular septum continuity, the developmental state of bicuspid valve and aorta petal etc. can observed and recognize to this tangent plane.
The 6th step, treat that the tangent plane sample is air-dry after, to the colouring of the emphasis anatomical structure that shown, treat to smear animal glue after the pigment drying, use lucite box fixed preparation, encapsulation again, inject 5% formalin solution at last and seal preservation.
Said emphasis anatomical structure is the pathological structure of the congenital heart disease that can reflect on the left ventricular outflow tract view tangent plane in above-mentioned the 6th step; To different congenital heart diseases, can make the corresponding different congenital heart disease teaching specimen of left ventricular outflow tract view tangent plane to different emphasis anatomical structure colourings.
For example: can observe and recognize damaged interventricular septum on this left ventricular outflow tract view tangent plane, carry out red-label and can produce ventricular septal defect congenital heart disease left ventricular outflow tract view tangent plane teaching specimen; On the left ventricular outflow tract view tangent plane, demonstrate two main arteries origin and out of shape unusual, carry out the congenital heart disease left ventricular outflow tract view tangent plane teaching specimen that red-label can be produced double outlet of right ventricle.
Above embodiment is the preferred embodiment of left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen of the present invention, and any simple substitution of on basis of the present invention, making all will fall within protection scope of the present invention.
Claims (3)
1. left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen may further comprise the steps:
1) the selection of material is the induced labor stillborn foetus of heart development deformity, and prepares impregnating fluid;
2) umbilical cord is repaiied cut neatly, comprise a bigger umbilical vein and two less arteria umbilicalis tangent planes on the umbilical cord cross section, umbilical vein catheterization perfusion impregnating fluid;
3) after sample successfully pours into, place week age, treat that impregnating fluid is penetrated into each one of whole body;
4) make free heart lung preparation, connective tissue is divested totally, flowing water flushing, dried for standby;
5) make the left ventricular outflow tract view of fetus suffered from congenital heart disease sample; Concrete operations are following: with the starting point of pulmonary conus as section; Make the axis of the transverse axis and the heart of tangent plane, upwards through aorta ascendens to superior vena cava, make a tangent plane through the through apex of anterior interventricular groove downwards;
6) treat that the tangent plane sample is air-dry after, to the colouring of the emphasis anatomical structure that shown, treat to smear animal glue after the pigment drying, use transparent box body fixed preparation, encapsulation again, inject 5% formalin solution at last and seal preservation.
2. left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is damaged interventricular septum structure in the described step 6).
3. left ventricular outflow tract view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is two a main arteries origin and out of shape in the described step 6).
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101915721A CN101779622B (en) | 2009-11-25 | 2009-11-25 | Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease |
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| CN2009101915721A CN101779622B (en) | 2009-11-25 | 2009-11-25 | Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease |
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| CN101779622A CN101779622A (en) | 2010-07-21 |
| CN101779622B true CN101779622B (en) | 2012-11-21 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101739880B (en) * | 2009-11-19 | 2015-01-14 | 裴秋艳 | Pretreatment method of heart of foetus |
| GB2492115B (en) * | 2011-06-22 | 2014-03-05 | Royal Brompton & Harefield Nhs Foundation Trust | Simulation apparatus |
| CN115132046B (en) * | 2022-07-07 | 2023-08-11 | 中山大学 | Functional anatomy inspection method for right ventricular outflow tract |
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2009
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Non-Patent Citations (6)
| Title |
|---|
| 低龄胎儿标本的制作;周郴龙;《实验教学与仪器》;20011231(第4期);第29页 * |
| 周郴龙.低龄胎儿标本的制作.《实验教学与仪器》.2001,(第4期),第29页. |
| 心脏腔内结构标本设计与制作;高叶梅等;《卫生职业教育》;20061231(第10期);第137页 * |
| 示教心脏标本改良剖切法;蔡雪彦等;《解剖学杂志》;20061231;第29卷(第2期);第262-264页 * |
| 蔡雪彦等.示教心脏标本改良剖切法.《解剖学杂志》.2006,第29卷(第2期),第262-264页. |
| 高叶梅等.心脏腔内结构标本设计与制作.《卫生职业教育》.2006,(第10期),第137页. |
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