CN101779621B - Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease - Google Patents
Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease Download PDFInfo
- Publication number
- CN101779621B CN101779621B CN 200910191570 CN200910191570A CN101779621B CN 101779621 B CN101779621 B CN 101779621B CN 200910191570 CN200910191570 CN 200910191570 CN 200910191570 A CN200910191570 A CN 200910191570A CN 101779621 B CN101779621 B CN 101779621B
- Authority
- CN
- China
- Prior art keywords
- heart
- apical
- fetus
- suffered
- heart disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for preparing a teaching specimen of an apical four-chamber view of a stillbirth suffered from a congenital heart disease. The method comprises the following steps: selecting an artificial labored fetus having a malformed heart; antiseptically treating the artificial labored fetus to prepare a free heart specimen, and making the vertical axis of the view parallel to the long axis of the heart and making the horizontal axis of the view parallel to the left-right direction of the heart from the apex of the heart,; and along the direction from the front upper position of 1 to 2 centimeters of left and right edges of the heart to the base of the heart, cutting the heart from the center of the right atrium and a position nearby an orifice of a pulmonary vein of the left atrium. The obtained apical four-chamber view specimen has the advantages of integrated and complete structures of all parts, no damage to microstructures in the heart, the same shape as that in a real living body, and capability of meeting the requirements of medical teaching and practice and the like, and is suitable for schools, hospitals or medical research institutes.
Description
Technical field
The present invention relates to the preparation method of teaching specimen, be specifically related to a kind of apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen.
Background technology
It is reported; Children with congenital heart disease accounts for 8%～12% of China's natus; Mean that there is the birth of 120,000～200,000 children with congenital heart disease every year in China, wherein complicated, at present treatment means still can not reach good result of treatment or the early stage dead children with congenital heart disease in birth back accounts for 20%, be the neonate and the childhood one of major causes of death; Brought heavy spirit and financial burden for family and society, bring endless misery to infant.Ultrasonic examination almost is the unique selection of the antenatal diagnostic imaging of heart of fetus deformity.Therefore, carrying out heart of fetus ultrasonic examination is the important component part of antenatal ultrasonic monitoring prevision foetus health, and it meets improving the overall quality of newborns of China, reduces the prenatal and postnatal care state basic policy of low age death rate.
The heart of fetus deformity is divided into lethal deformity and non-lethality deformity; Lethal heart malformations complexity is high, so heart of fetus ultrasonic examination is a technology that training property is stronger; Content is complicated, has comprised rudimentary knowledge such as human dissection, pathological anatomy, embryo growth and development and pathologic, physiologic.
Though most of ultrasonic doctors have ultrasound medicine basis and Ultrasonic Diagnosis experience for many years preferably now; But the basic theory of relevant heart of fetus Ultrasonic Diagnosis is weak; Standardization tangent plane and anatomical structure to heart of fetus lack understanding; And blindly inspection indeterminate to the ultrasonographic content of heart of fetus; The heart special construction that can reflect the congenital heart disease of tricuspid atresia, Ebstein deformity, ventricle dysplasia and holocentric chamber on anxious of the apical four-chamber; Therefore the apical four-chamber view of fetus suffered from congenital heart disease teaching specimen can reflect the heart special construction of the congenital heart disease of tricuspid atresia, Ebstein deformity, ventricle dysplasia and holocentric chamber intuitively, for teaching research provides reference.
Summary of the invention
The purpose of this invention is to provide the apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen; Utilize this method can produce anxious sample of apical four-chamber of the heart of fetus of suffering from congenital heart disease, each bilge construction is complete complete, does not damage the inner micro-structure of heart; Can reflect anxious special construction of going up congenital heart disease of apical four-chamber intuitively; And sample has the light cleaning, characteristics with long preservation period, and being used to impart knowledge to students to reach the effect of intuitivism apprehension.
For achieving the above object, technical scheme proposed by the invention is: the apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen may further comprise the steps:
1) the selection of material is the induced labor stillborn foetus of heart development deformity, and prepares impregnating fluid;
2) umbilical cord is repaiied cut neatly, comprise a bigger umbilical vein and two less arteria umbilicalis tangent planes on the umbilical cord cross section, umbilical vein catheterization perfusion impregnating fluid;
3) after sample successfully pours into, place week age, treat that impregnating fluid is penetrated into each one of whole body;
4) make free heart lung preparation, connective tissue is divested totally, flowing water flushing, dried for standby;
5) make the apical four-chamber view of fetus suffered from congenital heart disease sample, concrete operations are following: begin from apex, make the longitudinal axis of tangent plane parallel with long axis of heart, the right and left bit parallel of the transverse axis of tangent plane and heart; 1～2cm place, front upper place of edge along the heart left and right sides, bottom direction is entad broken off heart near the pulmonary vein inlet of the middle part of atrium dextrum and atrium sinistrum;
6) treat that the tangent plane sample is air-dry after, to the colouring of the emphasis anatomical structure that shown, treat to smear animal glue after the pigment drying, use transparent box body fixed preparation, encapsulation again, inject 5% formalin solution at last and seal preservation.
Adopt the technique effect that technique scheme reached to be: anxious each bilge construction of sample of resulting apical four-chamber is complete complete; Do not damage the inner micro-structure of heart, with truly in vivo shape is identical, consistent with ultrasonic examination standard tangent plane structure; The sample light is clean; But long preservation, cardiac ultrasonic dissec fabrication cycle is short, and is superior in quality.Problems such as present fetal ultrasound cardiogram teaching is abstract, thereby the complicacy teaching efficiency is bad, influence application that it has solved; It is easy to have method, and Production Time is short, and transparence is effective; Be well positioned to meet the advantage that medical teaching and practice etc. need, be suitable for school, hospital or medical research mechanism.
Further, the emphasis anatomical structure is that the attachment point of cuspis medialis vlvulae tricuspidalis moves down in the described step 6).Carry out red-label and can produce anxious teaching specimen of Ebstein deformity congenital heart disease apical four-chamber.
Further, the emphasis anatomical structure is that tricuspid valve is membrane thickness, aperture locking, septal defect in the described step 6).Carry out red-label and can produce anxious teaching specimen of congenital heart disease apical four-chamber of tricuspid atresia.
Further, the emphasis anatomical structure is that the left and right ventricles ratio is obviously not normal in the described step 6).Carry out red-label and can produce anxious teaching specimen of congenital heart disease apical four-chamber of ventricle dysplasia.
Further, the emphasis anatomical structure is that ventricle is single chamber in the described step 6).Carry out red-label and can produce anxious teaching specimen of holocentric chamber congenital heart disease apical four-chamber.
Further, the emphasis anatomical structure is that a side ventricle is residual cavity in the described step 6).Carry out red-label and can produce anxious teaching specimen of holocentric chamber congenital heart disease apical four-chamber.
Embodiment
We can know by anatomical knowledge, and atrium dextrum, chamber are positioned at the right front on room, interventricular septum plane, and right ventricle is the chambers of the heart of forefront, and the atrium dextrum is the chambers of the heart the most on the right side, constitute heart right border; Atrium sinistrum, chamber are positioned at the left back on room, interventricular septum plane, and the atrium sinistrum is the chambers of the heart at rear, and left ventricle is the chambers of the heart of side of keeping left most, constitutes left heart edge.The primary section of cardiac structure is three mutual rectangular sections along its major axis and minor axis, the i.e. section of sagittal section of the horizontal section of long axis of heart, long axis of heart (anteroposterior position section) and heart minor axis.The major axis of heart is the line between apex and the heart bottom central; The minor axis of heart is another axis that at right angles intersects with its major axis.Cut heart according to each standard tangent plane of cardiac ultrasonic inspection respectively, the knife edge is sharp keen when making tangent plane, guarantees neat in edge; Disposable success.The concrete operations step is following:
The first step, the selection of material are the induced labor stillborn foetus of heart development deformity, and prepare 10% formalin solution as impregnating fluid.
Second goes on foot, and umbilical cord is repaiied cut neatly, comprises a bigger umbilical vein and two less arteria umbilicalis tangent planes on the umbilical cord cross section, adopts the well-bucket perfusion to carry out the umbilical vein catheterization perfusion.
In the 3rd step, after sample successfully pours into (under the unblocked situation of umbilical vein, get final product in general 3 hours), approximately pass through the week age impregnating fluid and may penetrate into the whole body each several part; In order to next step operation.
The 4th step, produce free heart lung preparation (prop up about the descending aorta of reservation certain-length and pulmonary artery, show) with convenient observation and structure, connective tissue is divested totally, flowing water washes, dried for standby.
The 5th step, make the apical four-chamber view of fetus suffered from congenital heart disease sample, concrete operations are following: four chambers of the heart tangent planes of heart, begin from apex, make the longitudinal axis of tangent plane parallel, the right and left bit parallel of the transverse axis of tangent plane and heart with long axis of heart.1～2cm place, front upper place of edge along the heart left and right sides, bottom direction is entad broken off heart near the pulmonary vein inlet of the middle part of atrium dextrum and atrium sinistrum.Developmental states such as venous drainage, position, atrium, the chamber order of connection, four chambers of the heart chamber ratios, chamber valve and pericardium can observed and recognize to this tangent plane.Its upper limb of atrium dextrum that on four chambers of the heart sections of heart, is shown is the middle part of upper and lower vena cave rear wall, i.e. the sinus of venae cavae of atrium dextrum, inner wall smooth, the cross section of the visible boundary of the right-hand member of upper limb ridge.Right border is the right side wall of atrium dextrum, and lining endothelium is uneven because of there being musculi pectinati to join cloth.Left border is the right flank of atrial septum, just the middle part through fossa ovalis.The left surface of atrial septum lower edge links to each other with aortic cusp with right fibrous trigone; The chamber portion and the tricuspid septal cusp of its right flank and interventricular septum join; Therefore; The attachment point that demonstrates cuspis medialis vlvulae tricuspidalis on the tangent plane is more on the low side than the position of aortic cusp attachment point, and this is the important symbol of the left and right atrioventricular valve of difference on four chambers of the heart tangent planes.Lower edge is the lower wall of atrium dextrum, and its front end is a membranous part of interventri cular septum, and the rear end is an orifice of coronary sinus.Right ventricle is positioned at the lower right of tangent plane, is equivalent to back 1/3 place of right ventricle; Upper limb is a right atrioventricular orifice, and right border is the right side wall of right ventricle, and its lining endothelium is because of being imbued with the coarse injustice of myocardium girder.The right border of right ventricle is a right atrioventricular groove with the right border joint of atrium dextrum upwards, the cross section of visible arteria coronaria dextra in the ditch.Left border is an interventricular septum.The atrium sinistrum is horizontal oval, is positioned at the left ventricle top, divides atrium and auricle two parts.Left ventricle is positioned at the lower left, and the position of tangent plane is equivalent to the inclined to one side rear at left ventricle middle part, and the suitable for reading of tangent plane is the left ventricle mouth, ovalize, and it right 1/3 is the interventricular septum upper limb, all the other 23 are the left side coronary sulcus; Left atrioventricular orifice is divided into two parts by aortic cusp: right front portion is a left ventricular outflow tract view, and is identical with aortic orifice; Left rearward is that left chamber flows into the road, i.e. left atrioventricular orifice, and this tangent plane is positioned at this one just.
The 6th step, treat that the tangent plane sample is air-dry after, to the colouring of the emphasis anatomical structure that shown, treat to smear animal glue after the pigment drying, use lucite box fixed preparation, encapsulation again, inject 5% formalin solution at last and seal preservation.
Said emphasis anatomical structure is the pathological structure of the congenital heart disease that can reflect on anxious of the apical four-chamber in above-mentioned the 6th step; To different congenital heart diseases, can make the different congenital heart disease teaching specimen of anxious correspondence of apical four-chamber to different emphasis anatomical structure colourings.
For example: the attachment point that demonstrates cuspis medialis vlvulae tricuspidalis on anxious of this apical four-chamber moves down, and carries out red-label and can produce anxious teaching specimen of Ebstein deformity congenital heart disease apical four-chamber; On anxious of apical four-chamber, demonstrate tricuspid valve and be membrane thickness, aperture locking, septal defect, carry out anxious teaching specimen of congenital heart disease apical four-chamber that red-label can be produced tricuspid atresia; It is obviously not normal on anxious of apical four-chamber, to demonstrate the left and right ventricles ratio, carries out anxious teaching specimen of congenital heart disease apical four-chamber that red-label can be produced ventricle dysplasia; On anxious of apical four-chamber, demonstrate ventricle and be single chamber, carry out red-label and can produce anxious teaching specimen of holocentric chamber congenital heart disease apical four-chamber; On anxious of apical four-chamber, demonstrate a side ventricle and be residual cavity, carry out red-label and can produce anxious teaching specimen of holocentric chamber congenital heart disease apical four-chamber.
Above embodiment is the preferred embodiment of apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen of the present invention, and any simple substitution of on basis of the present invention, making all will fall within protection scope of the present invention.
Claims (6)
1. apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen may further comprise the steps:
1) the selection of material is the induced labor stillborn foetus of heart development deformity, and prepares impregnating fluid;
2) umbilical cord is repaiied cut neatly, comprise a bigger umbilical vein and two less arteria umbilicalis tangent planes on the umbilical cord cross section, umbilical vein catheterization perfusion impregnating fluid;
3) after sample successfully pours into, place week age, treat that impregnating fluid is penetrated into each one of whole body;
4) make free heart lung preparation, connective tissue is divested totally, flowing water flushing, dried for standby;
5) make the apical four-chamber view of fetus suffered from congenital heart disease sample, concrete operations are following: begin from apex, make the longitudinal axis of tangent plane parallel with long axis of heart, the right and left bit parallel of the transverse axis of tangent plane and heart; 1～2cm place, front upper place of edge along the heart left and right sides, bottom direction is entad broken off heart near the pulmonary vein inlet of the middle part of atrium dextrum and atrium sinistrum;
6) treat that the tangent plane sample is air-dry after, to the colouring of the emphasis anatomical structure that shown, treat to smear animal glue after the pigment drying, use transparent box body fixed preparation, encapsulation again, inject 5% formalin solution at last and seal preservation.
2. apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is that the attachment point of cuspis medialis vlvulae tricuspidalis moves down in the described step 6).
3. apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is that tricuspid valve is membrane thickness, aperture locking, septal defect in the described step 6).
4. apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is that the left and right ventricles ratio is not normal in the described step 6).
5. apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is that ventricle is single chamber in the described step 6).
6. apical four-chamber view of fetus suffered from congenital heart disease method for preparing teaching specimen as claimed in claim 1 is characterized in that: the emphasis anatomical structure is that a side ventricle is residual cavity in the described step 6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910191570 CN101779621B (en) | 2009-11-25 | 2009-11-25 | Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910191570 CN101779621B (en) | 2009-11-25 | 2009-11-25 | Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101779621A CN101779621A (en) | 2010-07-21 |
| CN101779621B true CN101779621B (en) | 2012-11-21 |
Family
ID=42519874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910191570 Expired - Fee Related CN101779621B (en) | 2009-11-25 | 2009-11-25 | Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101779621B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101739880B (en) * | 2009-11-19 | 2015-01-14 | 裴秋艳 | Pretreatment method of heart of foetus |
-
2009
- 2009-11-25 CN CN 200910191570 patent/CN101779621B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 低龄胎儿标本的制作;周郴龙;《实验教学与仪器》;20011231(第4期);第29页 * |
| 周郴龙.低龄胎儿标本的制作.《实验教学与仪器》.2001,(第4期),第29页. |
| 心脏腔内结构标本设计与制作;高叶梅等;《卫生职业教育》;20061231;第24卷(第10期);第137页 * |
| 示教心脏标本改良剖切法;蔡雪彦等;《解剖学杂志》;20061231;第29卷(第2期);第262-264页 * |
| 蔡雪彦等.示教心脏标本改良剖切法.《解剖学杂志》.2006,第29卷(第2期),第262-264页. |
| 高叶梅等.心脏腔内结构标本设计与制作.《卫生职业教育》.2006,第24卷(第10期),第137页. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101779621A (en) | 2010-07-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Crick et al. | Anatomy of the pig heart: comparisons with normal human cardiac structure | |
| Freedom et al. | The morphological spectrum of ventricular noncompaction | |
| CN101779622B (en) | Method for preparing teaching specimen of left ventricular outflow tract view of fetus suffered from congenital heart disease | |
| CN101779621B (en) | Method for preparing teaching specimen of apical four-chamber view of fetus suffered from congenital heart disease | |
| Wang et al. | Cardiovascular cast model fabrication and casting effectiveness evaluation in fetus with severe congenital heart disease or normal heart | |
| CN101810159B (en) | Method for preparing fetal congenital heart disease aortic arch long-axis section teaching specimen | |
| Dmitrieva et al. | The coronary sinus and adjacent structures in human adult hearts: morphometrical cadaveric anatomy | |
| Kilner et al. | Cardiovascular cavities cast in silicone rubber as an adjunct to post-mortem examination of the heart | |
| CN211349868U (en) | Ultrasonic teaching model for fetal heart and great vessel | |
| FYFE et al. | Transesophageal echocardiography for congenital heart disease | |
| CN101739880B (en) | Pretreatment method of heart of foetus | |
| Mądry et al. | Supravalvar mitral ring with a parachute mitral valve and subcoarctation of the aorta in a child with hemodynamically significant VSD. A study of the morphology, echocardiographic diagnostics and surgical therapy | |
| Trainini et al. | Fulcrum and torsion of the helical myocardium | |
| CN114677895B (en) | Manufacturing method of heart ultrasonic standardized human body model and human body model system | |
| Scheuermann | The roentgenological picture of the normal and the pathologic sella turcica | |
| Gómez et al. | Morphological expression of the pig coronary sinus and its tributaries: a comparative analysis with the human heart | |
| RU2766761C1 (en) | Method for making cast of left atrium in diastole phase | |
| CN113611201B (en) | Method for constructing aortic dissection model by adopting biological material | |
| Esmaeilzadeh et al. | Aortic atresia with normally developed left ventricle in a young adult | |
| Rigby | Echocardiography and Doppler | |
| Yang et al. | Bedside echocardiography-based hemodynamic assessment in the intensive care unit | |
| Pickoff et al. | Pulsed Doppler echocardiographic findings in total anomalous pulmonary venous drainage to the coronary sinus | |
| Pei et al. | Construction of a three-dimensional anatomical database of the human fetal heart | |
| Hung et al. | Prenatal diagnosis of dextrotransposition of the great arteries | |
| Liu et al. | Ultrasonic Frame of Reference for the Downward Displacement of the Posterior and Anterior Leaflets of the Tricuspid Valve in Children |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121121 Termination date: 20171125 |