CN1580243A - Rhodo streptomycete and its spirillicide - Google Patents
Rhodo streptomycete and its spirillicide Download PDFInfo
- Publication number
- CN1580243A CN1580243A CN 200410055326 CN200410055326A CN1580243A CN 1580243 A CN1580243 A CN 1580243A CN 200410055326 CN200410055326 CN 200410055326 CN 200410055326 A CN200410055326 A CN 200410055326A CN 1580243 A CN1580243 A CN 1580243A
- Authority
- CN
- China
- Prior art keywords
- streptomyces violaceoruber
- powder
- plant
- streptomyces
- violaceoruber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a Streptomyces violaceoruber and its molluscicides, wherein said Streptomyces violaceoruber is Streptomyces violaceoruber I which is preserved at China typical culture preservation center (CCTCC) with a preservation identification serial number of M203102 and a preservation date at 20030803. The bacterial strain is separated and activated by adopting Gao synthesized number 1 vegetable gelatine flat plate or inclined surface culture medium, and the Streptomyces violaceoruber bacterium solution with molluscidal functions is then cultured through fermentation by using a swing bottle; the Streptomyces violaceoruber bacterium solution and the plant pelleting additive are mixed to constitute a molluscicide. The Streptomyces violaceoruber in accordance with the present invention possesses stronger molluscidal effect, and the molluscicide formulated by the Streptomyces violaceoruber bacterium solution and plant pelleting additive possesses the following advantages: 1, without toxic and side effects and pollutions for the environment, it can be naturally degraded rapidly; 2, without toxic and side effects for persons and animals, it can maintain a long molluscidal time (longer than 15 days); 3, it is convenient to store, convey and use; 4, it has more improved molluscidal effect than pure bacterium solution and pure bacterium powder, with reduced drug amount; 5, relative pure plant powder, it has improved medical effects and prolonged time for maintaining the medical effects.
Description
Technical field
The present invention relates to a kind of microorganism and kill the spiral shell preparation, a kind of specifically Streptomyces violaceoruber and snail killing agent thereof with oncomelania effect extremely.
Background technology
Oncomelania is bilharzial intermediate host, is the important media that infects schistosomicide, thereby to kill oncomelania be one of effective way of control schistosomicide; The employed snail killing agent in present countries in the world, mostly be chemical snail killing agent, sodium pentachlorophenate as early stage use, the bromoacetamide of applying in recent years and nicotinanilide and niclosamide etc., these snail killing agents exist to some extent that selectivity is weak, persistence is poor, easily cause problem such as environmental pollution, for fear of the pollution of chemosynthesis molluscacide to environment, people seek out the pure natural plants snail killing agent always.From Archibsld (1933) goes out for the first time spiral shell with the fruit of Balanites aegyptiace since, have 1000 so far surplus kind of plant be used for snail killing test (Marstor, 1985; Amusan et al., 1995; Belot et al., 1993; Cepleanu et al., 1994; Yamamoto et al., 1996; El-Sawy et al., 1981; Mi Liuxi etc., 1997; Chen Guang, 1995; Sun Zhen is medium, and 1957), find that wherein 20 various plants have intensive molluscicide effect (Guo, 1987); And for example Wuhan plant research institute and Hubei Province's prevention and cure of schistosomiasis institute obtain the spiral shell rate of going out and reach 98.8% effect (Cui Tianyi etc., 1998) with Rhizome of Peltate Yam and the rhizome powder spiral shell that goes out; In recent years, the jointly tackling key problem of expert of units such as Wuhan City schistosomicide study on prevention institute, parasitosis teaching and research room of medical university of Tongji University, torrid zone institute of Heidelberg, Germany university, Wuhan City schistosomicide prevention and cure of endemic diseases office, obtain good effect (Gao Xia with " plant killer " Cortex jatrophae element spiral shell that goes out better than chemicals, 1999), though but dry product of plant or extract less contamination, but the same with the chemosynthesis molluscacide basically, effect is only disposable, effect can not be lasting, can not prevent (Li Shunyi etc., 1999 moved into of oncomelania; Horse peacefulness etc., 1999c, 2000), especially for beach, the river,lake and reservior continent oncomelania many Luo Qu of chance that move into, its expense money of taking a lot of work is just more difficult satisfactory with actual sn ail control effect.
In order to remedy the deficiency of these aspects of chemical snail killing agent, carry out the exploitation of biological snail-killing method research and biological molluscacide, screening is effectively killed and is shown necessary spiral shell microorganism day, and very meaningful to environment protection.
Through research worker's unremitting searching and experiment for many years, up to now, filtered out the microorganism strains that some can effectively kill oncomelania, and carried out the research of related fields, as convex pseudomonas (Psudomonas conrexa chester) is that Prevention ﹠ Cure Inst. for Parasitic Diseases, Hunan Prov. in 1967 isolates a strain Thp1 bacterial strain in the river snail body of living, 1971 again from natural mortality rate up to isolating a strain Lb bacterial strain 48% the ill oncomelania, these two bacterial strains are the convex pseudomonas of Rhodopseudomonas through identifying (Beijing institute of microbiology of the Chinese Academy of Sciences), snail killing experiment and result show: use infusion method and spray the infection oncomelania, any method no matter, the convex pseudomonas is killed the equal highly significant of effect of oncomelania, mortality of snails all reaches more than 90%, and infected oncomelania is generally most of dead in 2～4 days.But when using infusion method, every milliliter of bacterium liquid contains the bacterium number and need reach millions and (ten million/mL) time, just show sn ail control effect; Use spraying, every square metre contains the bacterium number and need reach TERA-SCALE (trillion/m
2) time, sn ail control effect is just stable.During sprinkling, when soil moisture content is lower than 45%, sn ail control effect descends;
And for example this bacterial strain of light gray streptomycete 230 (Streptomyces griseolus 230) be 1978 from the eyot soil of Lou Xi gulf, yueyang, hunan city an isolated strain be numbered 230 actinomyces strain, through being accredited as light gray streptomycete (Streptomycesgriseolus 230)
[2～4]Chamber and on-the-spot repetition test find that its culture all has the oncomelania effect of killing preferably, experimental results show that it kills the oncomelania effective ingredient is in mycelium, is indole carbazole and oxygen helerocyclics (C by experiment
28H
26N
4O
3).The spiral shell effective ingredient of killing of light gray streptomycete 230 is referred to as microbiotic 230.Microbiotic 230 kills the oncomelania effect and ambient moisture has certain relation.The high molluscicidal effect of humidity is good, particularly use back first day particularly important, this may be relevant soon with microbiotic 230 Decomposition in water, so toxic action was mainly being used back first day.But fishing gear there is certain toxicity;
The sn ail control effect of spiral shell effective microbe is killed in one strain, closely related with its application process, generally speaking, spiral shell must make microorganism contact just with breechblock and can give full play to effectiveness owing to go out, this point has greatly limited the application process of microorganism molluscacide, and because the strain of microorganism system is different, its biological characteristics is also different, simultaneously because the influence of many-sided chemical factors, temperature for example, illumination, soil moisture content, the formulation of molluscacide, pH value etc., therefore the microorganism spiral shell also not practical application of real meaning so far of going out, the great majority test is the laboratory, and minority is carried out the bacterial classification or the preparation of test in place, and the also mostly of use are meal, the on-the-spot sprinkling tested large usage quantity, but wherein the composition of substratum is more; Mostly employed in addition is on-the-spot direct isolating wild strain, without seed selection.And use the microbial preparation spiral shell that goes out, effectively the screening of bacterial classification is a critical step.In addition, the selection of its formulation, can reduce consumption, use microorganism snail killing agent and environment to mix also be the key that really use it to promote the use of.
Summary of the invention
The technical problem to be solved in the present invention is: a kind of Streptomyces violaceoruber and cultural method thereof of killing the spiral shell effect more by force that have is provided, and as the application and the good and practical Streptomyces violaceoruber snail killing agent of a kind of sn ail control effect of snail killing agent.
The technical scheme that the present invention solves the problems of the technologies described above is: a kind of spiral shell Streptomyces violaceoruber extremely, described Streptomyces violaceoruber (Streptomyces violaceoruber) is Streptomyces violaceoruber I (Streptomyces violaceoruber I), depositary institution is Chinese typical culture collection center (CCTCC), its deposit number is: M203102, preservation date are 20030803.
Cultivation and the fermentation process of described spiral shell Streptomyces violaceoruber extremely are as follows:
First: strain separating: getting frequently, ill oncomelania grinds after washing clean sterilization, be inoculated into after the 8-12 times of water dilution on synthetic No. 1 agar plate of Gao Shi or the slant medium, be inoculated on synthetic No. 1 agar plate of Gao Shi or the slant medium after maybe will diluting with 8-12 times of water from the soil of schistosomicide and the collection of non-schistosomiasis endemic district, 26-30 ℃ of cultivation, lucifuge is chosen individual plant and is separated bacterial classification after 48-96 hour; The second, spawn culture: will choose individual plant and separate bacterial classification and be inoculated into and make flat board or inclined-plane on synthetic No. 1 agar plate of Gao Shi or the slant medium and increase bacterium and cultivate 26-30 ℃ of cultivation, lucifuge, 48-96 hour; The 3rd, bacterium liquid shake flask fermentation is cultivated: with sterilization 300ml triangular flask, dress 120ml shake flask fermentation nutrient solution is got 0.5-1cm
2Flat board or inclined-plane increase bacterium activation bacterial strain, and gauze tampon envelope bottleneck shakes bottle rotating speed 120-180 rev/min, 26-28 ℃, 72-96 hour, must have the Streptomyces violaceoruber bacterium liquid of snail killing function;
Synthetic No. 1 agar plate of above-mentioned Gao Shi or slant medium are that Zulkovsky starch 8-12g, oatmeal 8-10g, saltpetre 1-2g, dipotassium hydrogen phosphate 0.5-1g, sal epsom 0.5-0.8mg, sodium-chlor 0.5-0.8g, ferrous sulfate 8-12mg, agar 18-22g and water 1000ml are mixed; The shake flask fermentation nutrient solution is with Zulkovsky starch, sucrose, lime carbonate, dipotassium hydrogen phosphate 0.5-1g, sal epsom 0.5-0.8mg, sodium-chlor 0.5-0.8g, oatmeal 8-10g, Semen Maydis powder, ammonium sulfate, yeast powder, vegetables oil, reaches water 1000ml mixing; Synthetic No. 1 agar plate of above-mentioned Gao Shi or slant medium and shake flask fermentation nutrient solution are transferred pH7.2～7.4 with sodium hydroxide or sodium bicarbonate, 110-131 ℃ of sterilization 20-30min, the cooling back is standby to room temperature.
Streptomyces violaceoruber utilizes its snail killing function and plant excipient to be mixed with the snail killing agent with good snail killing function as the application of snail killing agent specifically.
A kind of Streptomyces violaceoruber snail killing agent is mixed with the plant excipient by Streptomyces violaceoruber bacterium liquid and to form, wherein, and bacterium liquid: plant excipient=1000ml: 1000g.
Described plant excipient is to have the thick bits powder of oncomelania effect plant extremely, is about to plant stem-leaf, pericarp drying and grinding or microwave disinfection drying and grinding powdered that tool kills the oncomelania effect, and powder is crossed the 60-80 mesh sieve.
Described plant excipient with oncomelania effect extremely is the mixture that Folium seu Cortex Nerii powder, maple are raised leaf powder and ginkgo pericarp powder; Folium seu Cortex Nerii powder, maple raise the leaf powder and ginkgo pericarp powder weight ratio is 40: 30: 30.
Streptomyces violaceoruber of the present invention has snail killing function, has following advantage with the snail killing agent of its bacterium liquid and plant excipient preparation: 1, to environment have no side effect, pollution-free, natural degradation is rapid; 2, people and animals are had no side effect, keep the spiral shell time long (more than 15 days) of going out; 3, preservation, transportation, easy to use; 4, improve sn ail control effect than pure bacterium liquid, pure bacterium powder, reduced dosage; When 5, having improved drug effect and having prolonged effect than pure plant powder.
Further specify advantage of the present invention and purposes below in conjunction with embodiment.
Embodiment:
1 material and method
1.1 kill spirillum strain: Streptomyces violaceoruber I (Streptomyces violaceoruber I) is one of bacterial strain with strong molluscicidal effect that filters out from beach, continent, the oncomelania breeding place soil of lakeside, yueyang, hunan Dongting Lake, belong to actinomycetales (Actinomycetales), Streptomycetaceae (Streptomycetaceae), streptomyces (Streptomyces Waksman et al., 1943), Streptomyces violaceoruber (Streptomyces violaceoruber).
1.2 bacterial classification detects
Bacterium colony: circle, protuberance varies in size, and is vertebration, and the visible central authorities of outward appearance are different with the edge; Spore chain 3-5 encloses spiral (Fig. 1); Aerial hyphae is in medium oatmeal (shallow cinder grey), yeast extract paste malt extract medium (central authorities' white, purple all around), czapek's solution growth luxuriant (lilac), go up growth generally at organic starch culture-medium (yellow) and inorganic starch culture-medium (purple), go up poor growth at gelatine culture (brown); Not having water-soluble pigment produces; The nitrate reduction positive is a little less than digestion maltose, semi-lactosi, seminose, lactose, aldehydic acid sodium, the sucrose; Produce H
2The S feminine gender.
1.3 substratum and method
Separate synthetic No. 1 agar plate of bacterial classification culture medium prescription: Gao Shi or slant medium
| Zulkovsky starch 10g | ?NaCl???????????0.5g |
| Oatmeal 10g | ?FeSO 4?????????10mg |
| ?KNO 3??????????1g | Agar 20g |
| ?K 2HPO 4???????0.5g | ?H 2O???????????1000ml |
| ?MgSO 4·7H 2O??0.5mg | Be cooled to after the room temperature standby |
| PH7.2～7.4 121 ℃ sterilization 30min |
Get ill oncomelania 5-10 frequently, wash clean back and rinse wash disinfection with 1 ‰ mercury aqueous solution top layers, rinse with distilled water and to wash the back and grind rapidly, 10 times of water dilution back streak methods are inoculated on above-mentioned flat board or the slant medium, or get 5g from beach, continent, the oncomelania breeding place soil of lakeside, yueyang, hunan Dongting Lake to fine earth put Erlenmeyer flask and add 45ml distilled water, fully be mixed, tilt-pour process is inoculated on above-mentioned flat board or the slant medium after doing continuous 10 times of dilutions, 28 ℃ of cultivations, lucifuge is chosen individual plant and is separated bacterial classification behind the 3d.
The inclined-plane increases bacterium (activation bacterial strain) and uses synthetic No. 1 agar plate of culture medium prescription: Gao Shi or slant medium
| Zulkovsky starch 10g | ?NaCl??????0.5g |
| Oatmeal 10g | ?FeSO 4????10mg |
| ?KNO 3????????????g | Agar 20g |
| ?K 2HPO 4????????0.5g | H 2O????????????1000ml |
| ?MgSO 4·7H 2O???0.5mg | Be cooled to after the room temperature standby |
| PH7.2～7.4 121 ℃ sterilization 30min |
To choose individual plant separates bacterial classification and is inoculated on the above-mentioned substratum 28 ℃, 72h-96h, normal pressure ventilation.
Kill spirillum strain shake flask fermentation culture medium prescription:
| A solubility shallow lake part 12g | (NH 4) 2SO 4???2g |
| Sucrose 6g | Yeast powder 6g |
| ?CaCO 3??????????3g | Vegetables oil 2ml |
| ?K 2HPO 4????????1g | ?H 2O???????????1000ml |
| ?MgSO 4·7H 2O???1g | |
| ?NaCl?????????????1g | |
| Oatmeal 18g | |
| Semen Maydis powder 18g |
Use NaHCO
3Or NaOH accent pH7.2-7.4,121 ℃ of sterilization 30min inoculate after the cooling room temperature; Sterilization 300ml drop bottle, dress 120ml shake flask fermentation nutrient solution, 0.5cm
2Flat board or slant activation bacterial strain, gauze tampon envelope bottleneck shakes 120 rev/mins of bottle rotating speeds, and 28 ℃, 72-96h.Contain the bacterium number: 5,000 ten thousand-100,000,000 bacterium/1ml.
2 experimental results
2.1 water logging molluscicidal effect
Get bacterium liquid behind the above-mentioned shake flask fermentation 4d of 2ml, splash in the 998ml dechlorination water beaker and stir, soak oncomelania test extremely in the Streptomyces violaceoruber liquid chamber, 28 ℃ of room temperatures, (every sample is handled 30 spiral shells, and experiment repeats 3 mean values)
| Reagent concentration (‰) | Mortality of snails (%) | ||
| ????24h | ????48h | ????72h | |
| Streptomyces violaceoruber liquid 2 | ????10 | ????90 | ????95 |
| Napcp?10ppm | ????85 | ????100 | ????100 |
| The contrast of dechlorination water | ????0 | ????1 | ????1 |
Find in the experiment to reach millions (during as 3-5 10,000,000 bacterium/ml), show sn ail control effect, (hundred million/ml) time, effect significantly is 100%, dead spiral shell mensuration hammering method to reach hundred million grades when every milliliter of bacterium liquid contains the bacterium number.
2.2 bacterium powder spray effect is slightly carried at the scene
Test in place is puddles of water place outside the three groups of dikes in Tian Xing township, eastern suburb, Wuhan City, selects the higher area of oncomelania density, and experimental period is the 10-11 month in 2003, between temperature 16-24 ℃, and soil table pH7.5, moist, soil moisture content reaches 40%.After Streptomyces violaceoruber is cultivated 3-5d, mix husky vacuum-drying (W: W=100ml: 100g), sand bacterium powder 30g/m
2, spray 10m
23d, 7d are tested, 12d, 15d add up dead spiral shell number in sample ground, and molluscicidal effect is not obvious, and dead spiral shell absolute number is not high, and 15d is the highest only to be reached about 20%, but it is more obvious to press down spiral shell, the not busy operculums of promptly most spiral shells, and outage is stopped eating.
3 discuss
3.1 Streptomyces violaceoruber I water logging experiment molluscicidal effect is obvious, open and imitate soon, molluscicidal effect can reach more than 90% behind the 48h, but requires water logging to kill containing the bacterium number and need reaching 3,000 ten thousand bacterium/1ml and just show molluscicidal effect of spirillum liquid, reach the above spirillum liquid that kills of 5,000 ten thousand bacterium/1ml, molluscicidal effect is remarkable.
3.2 Streptomyces violaceoruber I coarse sand stirring vacuum drying (50 ℃ with interior oven dry) bacterium powder, on-the-spot molluscicidal effect is not good, though mixing, sand can reach the purpose of evenly splashing, but the performance of bacterium powder is renderd a service and also need be borrowed moist and the water propagation, and especially on the difficult waterline in continent, visible coarse sand bacterium powder is if be spilled into waterline place, waterside in the experiment, molluscicidal effect is significantly better than the nonirrigated farmland, be spilled into the visible spiral shell effect that obviously presses down in the careless beach, promptly spiral shell generally closes operculum, and it is inactive to creep.
Use above-mentioned Streptomyces violaceoruber bacterium liquid molluscicidal effect good, but its consumption is big, transportation, use inconvenience; Then sn ail control effect is not good enough with the bacterium powder.Owing to kill the spiral shell stem leaves of plants, the existing people's test of thick crumbs powder such as pericarp, to use consumption big but crude extract kills spiral shell, and effect is not good enough, and source tannin plant resource is also sent one's regards to topic.Can reduce consumption, reinforced effects, both solved part source material difficulty, improve molluscicidal effect again, we as the attached shape thing that kills the spiral shell microbial inoculum, manufacture Powdered novel snail killing agent to plant powder for this reason, not only are convenient to transportation, packing, anti-storage, synergy also easy to use, test in place show that sn ail control effect is good.
The Streptomyces violaceoruber snail killing agent:
1 materials and methods
1.1 Streptomyces violaceoruber (Streptomyces violaceoruber I)
Improvement Gao Shi No. 1 shake-flask culture base, the 300ml triangular flask, the 100-120ml substratum of packing into, 150 rev/mins, 28 ℃ of lucifuges, aerated culture 4-7d results, microscopy 5,000 ten thousand bacterium are above/and ml gets final product.
1.2 Folium seu Cortex Nerii (Nerium undicum) maple is raised (Ptercarya stenoptera) ginkgo (Ginkgo biloba) fresh leaf, pericarp, stem epidermis microwave are dry rapidly.Grind respectively, powder gets the homogeneous powder by 60 mesh sieves (particle 0.25mm).50 ℃ must not be above 3% with interior vacuum-drying powder moisture.The Folium seu Cortex Nerii powder: maple is raised the leaf powder: ginkgo pericarp powder=(W.W.W weight ratio) 40: 30: 30
1.3 Streptomyces violaceoruber snail killing agent:
Kill spiral shell plant mixed powder end and mix at 1: 1 with Streptomyces violaceoruber liquid, promptly 1000ml bacterium liquid adds 1000g plant mixed powder, after fully stirring, and vacuum-drying 12h below 50 ℃, plastic closures packing, storage or rig-site utilization.
2 results
2.1 the spiral shell test is killed in the indoor immersion of Streptomyces violaceoruber snail killing agent:
Streptomyces violaceoruber snail killing agent 2 gram powders are poured in the 1000ml dechlorination water, and every beaker packing 100ml snail killing agent liquid is inserted one bag of negative one-tenth spiral shell, every packed 30 spiral shells, 28 ℃ of room temperatures., soak 24-72h, experiment repeats 4 times and averages, and the inspection comparative result is as follows:
| Snail killing agent concentration ‰ | Mortality of snails % 24h 48h 72h | ||
| Streptomyces violaceoruber stoste 2 | ????10 | ????90 | ????95 |
| Streptomyces violaceoruber snail killing agent pulvis 2 | ????90 | ????100 | ????100 |
| Napcp???10.0ppm | ????85 | ????100 | ????100 |
| Dechlorination water blank | ????0 | ????1 | ????1 |
Dead spiral shell check water is surveyed method and hammering method.
2.2 test in place and effect are relatively
30g/m
2The Streptomyces violaceoruber snail killing agent evenly sprays close ribbon on the moist waterline, or place, the close spiral shell in beach, river grass beach, looks into the spiral shell counting in the unit surface, and the indirect labor throws spiral shell, satisfies requirement of experiment; During test, when ground temperature 16-24 ℃, gentle breeze, weather mostly are cloudy day, fine, light rain, conversion is frequent; Test-results is as follows:
Date of test 2003.10.5---11.2, when ground temperature 16---24 ℃, soil table pH7.5.
| Snail killing agent | Consumption (g/m 2) | Spray area (m 2) | Oncomelania quantity (only) | The dead spiral shell rate of 3d (%) | The dead spiral shell rate of 7d (%) | The dead spiral shell rate of 15d (%) |
| Streptomyces violaceoruber (sand is mixed) | ????30 | ????3 | ????100 | ????2 | ????15 | ????20 |
| The Sweetscented Oleander Leaf meal | ????30 | ????3 | ????100 | ????16 | ????58 | ????75 |
| Maple is raised the leaf meal | ????30 | ????3 | ????100 | ????3 | ????15 | ????30 |
| Ginkgo pericarp meal | ????30 | ????3 | ????100 | ????3 | ????17 | ????54 |
| The Streptomyces violaceoruber snail killing agent | ????30 | ????3 | ????100 | ????26 | ????86 | ????92 |
| Napcp | ????10 | ????3 | ????100 | ????70 | ????95 | |
| Niclosamide | ????2 | ????3 | ????100 | ????52 | ????77 |
Test repeats secondary, and water seaoning test spiral shell anyway.
2.3 moist water logging soil table is good than thick grass nonirrigated farmland molluscicidal effect
Powdered medicament is sprinkled upon moist soil table or the soil table is soaked in watering every other day, can strengthen drug effect, increases molluscicidal effect, the soil table oncomelania after spiral shell plant powder and the Streptomyces violaceoruber snail killing agent is killed in sprinkling, majority closes the operculum outage, stops eating, and original position does not move in a couple of days, but when water logging detects, mostly be spiral shell alive, it is to press down spiral shell that visible biological snail killing agent at first acts on, along with fate after the dispenser increases, effective constituent is dissolved to the environment face of land, just sees molluscicidal effect after about 1 week more.
It is low that molluscicide of the present invention has a consumption, good drug efficacy, the characteristics that timeliness is long.
Claims (6)
1, a kind of Streptomyces violaceoruber, it is characterized in that described Streptomyces violaceoruber (Streptomyces violaceoruber) is Streptomyces violaceoruber I (Streptomyces violaceoruber I), depositary institution is Chinese typical culture collection center (CCTCC), its deposit number is: M203102, preservation date are 20030803.
2, the production method of a kind of Streptomyces violaceoruber is characterized in that its cultivation and fermentation process are as follows:
First: strain separating: getting frequently, ill oncomelania grinds after washing clean sterilization, be inoculated into after the 8-12 times of water dilution on synthetic No. 1 agar plate of Gao Shi or the slant medium, be inoculated on synthetic No. 1 agar plate of Gao Shi or the slant medium after maybe will diluting with 8-12 times of water from the soil of schistosomicide and the collection of non-schistosomiasis endemic district, 26-30 ℃ of cultivation, lucifuge is chosen individual plant and is separated bacterial classification after 48-96 hour; The second, spawn culture: will choose individual plant and separate bacterial classification and be inoculated into and make flat board or inclined-plane on synthetic No. 1 agar plate of Gao Shi or the slant medium and increase bacterium and cultivate 26-30 ℃ of cultivation, lucifuge, 48-96 hour; The 3rd, bacterium liquid shake flask fermentation is cultivated: with sterilization 300ml triangular flask, dress 120ml shake flask fermentation nutrient solution is got 0.5-1cm
2Flat board or inclined-plane increase bacterium activation bacterial strain, and gauze tampon envelope bottleneck shakes bottle rotating speed 120-180 rev/min, and 26-28 ℃, 72-96 hour; The Streptomyces violaceoruber bacterium liquid that must have snail killing function;
Synthetic No. 1 agar plate of above-mentioned Gao Shi or slant medium are that Zulkovsky starch 8-12g, oatmeal 8-10g, saltpetre 1-2g, dipotassium hydrogen phosphate 0.5-1g, sal epsom 0.5-0.8mg, sodium-chlor 0.5-0.8g, ferrous sulfate 8-12mg, agar 18-22g and water 1000ml are mixed; The shake flask fermentation nutrient solution is with Zulkovsky starch, sucrose, lime carbonate, dipotassium hydrogen phosphate 0.5-1g, sal epsom 0.5-0.8mg, sodium-chlor 0.5-0.8g, oatmeal 8-10g, Semen Maydis powder, ammonium sulfate, yeast powder, vegetables oil, reaches water 1000ml mixing; Synthetic No. 1 agar plate of above-mentioned Gao Shi or slant medium and shake flask fermentation nutrient solution are transferred pH7.2～7.4 with sodium hydroxide or sodium bicarbonate, 110-131 ℃ of sterilization 20-30min, the cooling back is standby to room temperature.
3, Streptomyces violaceoruber is as the application of snail killing agent.
4, a kind of Streptomyces violaceoruber snail killing agent, its feature are mixed with the plant excipient by Streptomyces violaceoruber bacterium liquid as claimed in claim 2 at described snail killing agent and form, wherein, and bacterium liquid: plant excipient=1000ml: 1000g;
5, purplish red mould snail killing agent as claimed in claim 4 is characterized in that described plant excipient is to have the thick bits powder of oncomelania effect plant extremely, and thick quiet powder is the 60-80 order.
6, purplish red mould snail killing agent as claimed in claim 4 is characterized in that described plant excipient with oncomelania effect extremely is the mixture that Folium seu Cortex Nerii powder, maple are raised leaf powder and ginkgo pericarp powder; Folium seu Cortex Nerii powder, maple raise the leaf powder and ginkgo pericarp powder weight ratio is 40: 30: 30.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410055326 CN1242054C (en) | 2003-12-26 | 2004-08-20 | Rhodo streptomycete and its spirillicide |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200310111659.6 | 2003-12-26 | ||
| CN200310111659 | 2003-12-26 | ||
| CN 200410055326 CN1242054C (en) | 2003-12-26 | 2004-08-20 | Rhodo streptomycete and its spirillicide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1580243A true CN1580243A (en) | 2005-02-16 |
| CN1242054C CN1242054C (en) | 2006-02-15 |
Family
ID=34593127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410055326 Expired - Fee Related CN1242054C (en) | 2003-12-26 | 2004-08-20 | Rhodo streptomycete and its spirillicide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1242054C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388362A (en) * | 2014-12-04 | 2015-03-04 | 江苏省血吸虫病防治研究所 | Strain of streptomyces nigrogriseolus capable of generating molluscicidal active substance and application of streptomyces nigrogriseolus |
| CN104450570A (en) * | 2014-12-04 | 2015-03-25 | 江苏省血吸虫病防治研究所 | Light streptomyces coelicolor for generating molluscicidal active substance and application of light streptomyces coelicolor |
| CN104498396A (en) * | 2014-12-04 | 2015-04-08 | 江苏省血吸虫病防治研究所 | Deep grey streptomyces caniferus for producing molluscicidal active substances and application thereof |
| CN104531557A (en) * | 2014-12-04 | 2015-04-22 | 江苏省血吸虫病防治研究所 | Streptomyces subrutilus capable of generating molluscicide active substance and application thereof |
| CN113621533A (en) * | 2021-07-09 | 2021-11-09 | 湖南师范大学 | Streptomyces rubiginosus Z1-26, microecological preparation and preparation method thereof |
-
2004
- 2004-08-20 CN CN 200410055326 patent/CN1242054C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388362A (en) * | 2014-12-04 | 2015-03-04 | 江苏省血吸虫病防治研究所 | Strain of streptomyces nigrogriseolus capable of generating molluscicidal active substance and application of streptomyces nigrogriseolus |
| CN104450570A (en) * | 2014-12-04 | 2015-03-25 | 江苏省血吸虫病防治研究所 | Light streptomyces coelicolor for generating molluscicidal active substance and application of light streptomyces coelicolor |
| CN104498396A (en) * | 2014-12-04 | 2015-04-08 | 江苏省血吸虫病防治研究所 | Deep grey streptomyces caniferus for producing molluscicidal active substances and application thereof |
| CN104531557A (en) * | 2014-12-04 | 2015-04-22 | 江苏省血吸虫病防治研究所 | Streptomyces subrutilus capable of generating molluscicide active substance and application thereof |
| CN104388362B (en) * | 2014-12-04 | 2017-01-11 | 江苏省血吸虫病防治研究所 | Strain of streptomyces nigrogriseolus capable of generating molluscicidal active substance and application of streptomyces nigrogriseolus |
| CN104498396B (en) * | 2014-12-04 | 2017-10-03 | 江苏省血吸虫病防治研究所 | One plant generation ovicidal trait material life dull gray streptomycete and its application |
| CN113621533A (en) * | 2021-07-09 | 2021-11-09 | 湖南师范大学 | Streptomyces rubiginosus Z1-26, microecological preparation and preparation method thereof |
| CN113621533B (en) * | 2021-07-09 | 2023-09-05 | 湖南师范大学 | Streptomyces erythropolis Z1-26, microecological preparation and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1242054C (en) | 2006-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103992968B (en) | Preparation method of household garbage deodorizing and sterilizing composite inoculant | |
| CN103555624B (en) | One strain fixed nitrogen Bacillus licheniformis and uses thereof | |
| CN102027998B (en) | Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof | |
| CN101974430A (en) | Environmentally-friendly microorganism preparation for eliminating house-fitment pollution and preparation method thereof | |
| CN101781569B (en) | Soil improvement and anti continuous cropping multifunctional biological agent | |
| CA2874890A1 (en) | Bio-available mineral fertilizer and derivative applications, including product processes | |
| CN105586298A (en) | Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide | |
| CN102765972B (en) | Organic garbage decomposing bacterium agent, as well as preparation method and application of organic garbage composting bacterium agent | |
| CN101518265A (en) | Paecilomyces cicadae biological bacteria preparation, method for producing same and application of same to plant nematode prevention | |
| CN101497542A (en) | Biological preparation containing plant growth promoting bacteria, as well as preparation and use thereof | |
| CN102180729A (en) | Strontium-containing organic microorganism fertilizer and preparation method thereof | |
| CN103484396A (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN105948842B (en) | Livestock and poultry dung recovery produces lichens micro-organisms bacillus fertilizer | |
| CN105475363A (en) | Cucumber growth promotion composite microbial agent and application thereof | |
| CN101781565B (en) | Multifunctional biological agent containing Trichoderma pseudokiningii | |
| CN101892173A (en) | Bacillus subtilis with bio-control effect on continuous cropping diseases of strawberries and application thereof | |
| CN102286400B (en) | Rice endotrophic azotobacter for improving disease resistance and stress resistance of crops and purpose thereof | |
| CN1242054C (en) | Rhodo streptomycete and its spirillicide | |
| CN103382449B (en) | Streptomyces sp. T2-10 with broad-spectrum insecticidal and antibacterial activity and its application | |
| CN105219674A (en) | A kind of hydrocarbon degradation bacteria and application thereof | |
| CN1597930B (en) | Yellow bacillus and its snail killing agent | |
| CN112725213A (en) | Arthrobacter and application thereof as decomposing agent for decomposing vegetable straws | |
| CN102242066B (en) | Acremonium hansfordii Ahy1 and Acremonium hansfordii wettable powder | |
| CN102618469B (en) | Rhodobacter capsulatus and application thereof | |
| CN106244486B (en) | The bacillus of one high-efficiency degradation nitrogen pollutant and its composite bacteria preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: HUBEI UNIVERSITY Free format text: FORMER OWNER: YANG JIANMING Effective date: 20070511 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20070511 Address after: 430062 Department of science and technology, Hubei University, Wuchang 11, Wuhan, Xueyuan Road, Hubei Patentee after: Hubei University Address before: 430062 School of life science, Hubei University, Wuchang 11, Wuhan, Hubei, Xueyuan Road Patentee before: Yang Jianming |
|
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |