CN104498396B - One plant generation ovicidal trait material life dull gray streptomycete and its application - Google Patents
One plant generation ovicidal trait material life dull gray streptomycete and its application Download PDFInfo
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- CN104498396B CN104498396B CN201410726401.5A CN201410726401A CN104498396B CN 104498396 B CN104498396 B CN 104498396B CN 201410726401 A CN201410726401 A CN 201410726401A CN 104498396 B CN104498396 B CN 104498396B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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Abstract
The invention discloses one plant of life dull gray streptomycete for producing ovicidal trait material and its application, belong to parasite control technical field.Actinomyces preserving number of the present invention is CGMCC No.9784, and its 16S rDNA sequence is as shown in SEQ ID No.1.The purposes of the bacterium tunning in molluscacide is prepared.It is an advantage of the invention that：Described actinomyces CGMCC No.9784 are that a streptomycete belongs to novel species, and its zymotic fluid has good killing action to oncomelania, with higher DEVELOPMENT PROSPECT.
Description
Technical field
The present invention relates to the streptomyces of one plant of generation ovicidal trait material（Streptomyces caniferus）Unwrapping wire
Bacterium, belongs to parasite control technical field.
Background technology
Japanese schistosomiasis is a kind of healthy and life security the great communicable disease of serious harm our people,
By preventing and treating for many years, achievement highly visible is achieved, but schistosomiasis propagation still exists with trend's elements, as causing
Schistosomiasis epidemic repeatedly or again popular potential threat factor.Oncomelania hupensis is unique intermediate host of Japanese schistosomiasis,
It is one of important measure of achievement that prevention and control schistosomiasis are popular, consolidate prevention and cure of snail fever obtains to kill oncomelania.Cause
It is still current urgent and important task that this, which finds newly efficient, low toxicity, inexpensive molluscacide,.
The development of current biogenic pesticide is paid close attention to by countries in the world, and existing multiple biological pesticide products, which are obtained, extensively should
With including jinggangmeisu, gibberellin, AVM, kasugarnycin etc..It is currently to grind that novel agrochemical is screened from actinomyces
One of focus studied carefully.
The microorganism of enormous amount is multiplied in existence in soil, is formed to adapt to the microorganism in ecological environment, soil
Unique physiological property and metabolic characteristic, and then diversified secondary metabolite is generated, find and open as the mankind
The important source of compound of the hair with bioactivity.
The content of the invention
The invention solves the problems that technology be to provide one plant and can produce the streptomycete of ovicidal trait material.
To achieve the above object, the present invention uses following technical scheme：
The streptomyces of one plant of generation ovicidal trait material（Streptomyces caniferus）Actinomyces, its preserving number
For CGMCC No.9784.
Actinomyces CGMCC No.9784 are isolated in Tonghua, Jilin Province city Yuhuang Mount soil.
The actinomyces CGMCC No.9784, its 16S rDNA sequence are as shown in SEQ ID No.1.
The application of the actinomyces CGMCC No.9784, the purposes of the bacterium tunning in molluscacide is prepared.
The actinomyces have following microorganism feature：
（1）Cultural characteristic and morphological feature
The bacterial strain is in potato dextrose agar（PDA）, Gause I synthetic media, glycerine asparagine fine jade
Fat culture medium（ISP2）, inorganic salts starch agar medium（ISP3）, yeastex malt saccharin agar medium（ISP4）, oat
Powder agar medium（ISP5）, well-grown on Czapek culture mediums, can produce more rich aerial hyphae, but without solvable
Property pigment produce.Cultural characteristic of the bacterial strain on different culture media refers to table 1.The bacterial strain is observed under an optical microscope
Fibrillae of spores is hooked, and spore is rounded.
The bacterial strain CGMCC No.9784 of table 1 cultural characteristic
（2）Physiological and biochemical property
The bacterial strain can utilize glucose, sucrose, maltose, mannose, mannitol；Can not using arabinose, xylose,
Fructose, lactose.Cellulose, no melanin and H can not be utilized2S is produced.Gelatin liquefaction can be made, milk, which does not solidify, not to be peptonized.
（3）The actinomyces, its 16S rDNA sequence is shown in SEQ ID No.1.
Beneficial effects of the present invention：Described actinomyces CGMCC No.9784 are that a streptomycete belongs to novel species, its zymotic fluid
There is good killing action to oncomelania, with higher DEVELOPMENT PROSPECT.
Biological material specimens preservation：The streptomyces of one plant of generation ovicidal trait material（Streptomyces caniferus）Actinomyces, have been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, referred to as
CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, deposit number CGMCC
No.9784, preservation date on October 16th, 2014.
Brief description of the drawings
Fig. 1 bacterial strains of the present invention are in Gause I culture medium（Culture 10 days）Culture form.
The mycelia of Fig. 2 bacterial strains of the present invention and spore filament shapes, 400 ×.
Embodiment
With reference to specific embodiment, the present invention is further illustrated
Embodiment 1：Bacterial strain CGMCC No.9784 separation
Weigh 1g soil samples to be added in the triangular flask of the physiological saline containing 10mL, fully mix.10 times of series ladders are made to sample
Degree dilution, respectively takes 100 μ L 10-1、10-2、10-3、10-4、10-5The bacterium solution of dilution factor is coated on Gause I culture medium, 28 DEG C
It is inverted culture.The different bacterium colony of in good time picking form, color carries out line purifying.For purified actinomyces, according to bacterium colony
Morphological feature, excludes identical bacterial strain, is transferred to the preservation of 4 DEG C of inclined-plane, and numbering gives over to bacterial strain screening use.
Embodiment 2：Bacterial strain CGMCC No.9784 culture
（1）Inclined-plane culture：Culture 7 days.Culture medium：Soluble starch 2%, potassium nitrate 0.1%, sodium chloride 0.05%, phosphoric acid hydrogen
Dipotassium 0.05%, magnesium sulfate 0.05%, ferrous sulfate 0.01%, agar 1.5%.Distilled water is prepared, initial pH 7.2-7.4.
（2）Fermented and cultured：From the activated single bacterium colony access fermentation medium of inclined-plane picking, 28 DEG C, 180r/min shakes
Bottle culture 7 days.Culture medium：Glucose 2%, soluble starch 2%, potassium nitrate 0.5%, dipotassium hydrogen phosphate 0.05%.Matched somebody with somebody with distilled water
System, initial pH 7.2-7.4.
The bacterial strain CGMCC No.9784 zymotic fluid molluscicidal effects of embodiment 3
By zymotic fluid with 0.22 μm of filtering with microporous membrane, filtrate is diluted to 1/10,1/50 and the 1/ of fermenation raw liquid respectively
100, each concentration is put into 10 oncomelanias into spiral shell, and every group of amount of liquid medicine is 100mL, soaks and decoction is removed after oncomelania 72h, clear water punching
Wash, recovery 48h, hammering method observation oncomelania life or death is simultaneously counted；Each concentration do three groups it is parallel while setting clear water control.Proferment
1/10,1/50 and 1/100 mortality of snails of liquid concentration is respectively 100.00%, 100.00% and 93.33%, shows this zymotic fluid
There is good molluscicidal effect.
<210> SEQ ID NO: 1
<211> 1479
<212> DNA
<213>Streptomyces（Streptomycescaniferus）Actinomyces CGMCC No.9784
<400>1
TCGAACGCTG GCGGCGTGCT TAACACATGC AAGTCGAACG ATGAACCTCC TTCGGGAGGG 60
GATTAGTGGC GAACGGGTGA GTAACACGTG GGCAATCTGC CCTTCACTCT GGGACAAGCC 120
CTGGAAACGG GGTCTAATAC CGGATACGAC CACCGACCGC ATGGTCTGGT GGTGGAAAGC 180
TCCGGCGGTG AAGGATGAGC CCGCGGCCTA TCAGCTTGTT GGTGGGGTGA TGGCCTACCA 240
AGGCGACGAC GGGTAGCCGG CCTGAGAGGG CGACCGGCCA CACTGGGACT GAGACACGGC 300
CCAGACTCCT ACGGGAGGCA GCAGTGGGGA ATATTGCACA ATGGGCGAAA GCCTGATGCA 360
GCGACGCCGC GTGAGGGATG ACGGCCTTCG GGTTGTAAAC CTCTTTCAGC AGGGAAGAAG 420
CGAGAGTGAC GGTACCTGCA GAAGAAGCGC CGGCTAACTA CGTGCCAGCA GCCGCGGTAA 480
TACGTAGGGC GCAAGCGTTG TCCGGAATTA TTGGGCGTAA AGAGCTCGTA GGCGGCTTGT 540
CACGTCGGAT GTGAAAGCCC GGGGCTTAAC CCCGGGTCTG CATTCGATAC GGGCAGGCTA 600
GAGTTCGGTA GGGGAGATCG GAATTCCTGG TGTAGCGGTG AAATGCGCAG ATATCAGGAG 660
GAACACCGGT GGCGAAGGCG GATCTCTGGG CCGATACTGA CGCTGAGGAG CGAAAGCGTG 720
GGGAGCGAAC AGGATTAGAT ACCCTGGTAG TCCACGCCGT AAACGTTGGG AACTTAGGTG 780
TGGGCGAATT CCACGTCGTC CGTGCCGCAG CTAACGCATT AAGTTCCCCG CCTGGGGAGT 840
ACGGCCGCAA GGCTAAAACT CAAAGGAATT GACGGGGGCC CGCACAAGCA GCGGAGCATG 900
TGGCTTAATT CGACGCAACG CGAAGAACCT TACCAAGGCT TGACATACAC CGGAAACGTC 960
TGGAGACAGG CGCCCCCTTG TGGTCGGTGT ACAGGTGGTG CATGGCTGTC GTCAGCTCGT 1020
GTCGTGAGAT GTTGGGTTAA GTCCCGCAAC GAGCGCAACC CTTGTTCTGT GTTGCCAGCA 1080
TGCCCTTCGG GGTGATGGGG ACTCACAGGA GACTGCCGGG GTCAACTCGG AGGAAGGTGG 1140
GGACGACGTC AAGTCATCAT GCCCCTTATG TCTTGGGCTG ACACGTGCTA CAATGGCCGG 1200
TACAATGAGC TGCGATACCG CGAGGTGGAG CGAATCTCAA AAAGCCGGTC TCAGTTCGGA 1260
TTGGGGTCTG CAACTCGACC CCATGAAGTC GGAGTTGCTA GTAATCGCAG ATCAGCATTG 1320
CTGCGGTGAA TACGTTCCCG GGCCTTGTAC ACACCGCCCG TCACGTCACG AAAGTCGGTA 1380
ACACCCGAAG CCGGTGGCCC AACCCCTTGT GGGAGGGAAT CGTCGAAGGT GGGACTGGCG 1440
ATTGGGACGA AGTCGTAACA AGGTAGCCGT ACCGGAAGG 1479
Claims (3)
1. the streptomyces of one plant of generation ovicidal trait material（Streptomyces caniferus）Actinomyces, its preserving number is
CGMCC No.9784。
2. actinomyces CGMCC No.9784 according to claim 1, it is characterised in that its 16S rDNA sequence such as SEQ
Shown in ID No.1.
3. actinomyces CGMCC No.9784 application described in claim 1, it is characterised in that the bacterium tunning kills spiral shell in preparation
Purposes in medicine.
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| CN201410726401.5A CN104498396B (en) | 2014-12-04 | 2014-12-04 | One plant generation ovicidal trait material life dull gray streptomycete and its application |
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| CN201410726401.5A CN104498396B (en) | 2014-12-04 | 2014-12-04 | One plant generation ovicidal trait material life dull gray streptomycete and its application |
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| CN104498396B true CN104498396B (en) | 2017-10-03 |
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| CN111088199B (en) * | 2020-02-14 | 2021-10-19 | 江南大学 | Streptomyces fuselaphus and application thereof in production of proteolytic enzyme |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580243A (en) * | 2003-12-26 | 2005-02-16 | 杨建明 | Rhodo streptomycete and its spirillicide |
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- 2014-12-04 CN CN201410726401.5A patent/CN104498396B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580243A (en) * | 2003-12-26 | 2005-02-16 | 杨建明 | Rhodo streptomycete and its spirillicide |
Non-Patent Citations (2)
| Title |
|---|
| 微生物灭钉螺研究现状;杨建明 等;《湖北大学学报( 自然科学版)》;20031230;第25卷(第4期);337-341 * |
| 杀螺微生物的分离与杀螺及抑菌活性的研究;张桂敏 等;《环境科学与技术》;20050731;第28卷(第4期);8-9 * |
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