CN1572138A - Ear signal for pig and livestock identifying method - Google Patents

Ear signal for pig and livestock identifying method Download PDF

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Publication number
CN1572138A
CN1572138A CN 200310123244 CN200310123244A CN1572138A CN 1572138 A CN1572138 A CN 1572138A CN 200310123244 CN200310123244 CN 200310123244 CN 200310123244 A CN200310123244 A CN 200310123244A CN 1572138 A CN1572138 A CN 1572138A
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China
Prior art keywords
ear
pig
ear tag
blade
axial region
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Pending
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CN 200310123244
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Chinese (zh)
Inventor
铃木安彦
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YOSHIMOTO MISUKE CO Ltd
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YOSHIMOTO MISUKE CO Ltd
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Publication of CN1572138A publication Critical patent/CN1572138A/en
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Abstract

The earmark for the pig is at least constituted of a first member A (a male member) having a flat flange part 3, and a shaft part 2 protruded from the flange part 3 and having a conical cusp part 1 formed at the tip position so as not to be separated, a second member B (a female member) having a hole part 4 to which the shaft part 2 passing through the ear of the livestock is attached, and a flat plate part 5 for nipping the ear by facing to the flange part 3 in an inserted state. The first member A is formed out of a flexible synthetic resin. The method for identifying the livestock comprises using the earmark for the pig, coloring each of the first member A and the second member B, and utilizing the combination of the colors for the identification of the livestock.

Description

Pig is with ear tag and domestic animal individual discrimination method
Technical field
The invention relates to ear tag (ear-tag) of the porker of delivering for sale after only being born 6-8 month and the domestic animal individual discrimination method that utilizes this ear tag.
Background technology
For realizing, in cattle breeding industry now, generally use ear tag (or " ear-tag ") to carry out provenance identification and individual management to ox, sheep etc., the particularly traceability of fat stock feeding process.This ear tag is installed on the domestic animal ear, is made by metal or synthetic resin usually.Particularly in the raising of milk cow, beef cattle and sheep, this type of ear tag is used widely.
This type of ear tag majority is made up of male member and female member.For perforate on ear easily, male member is provided with the axial region of bar-shaped or column, and its head end (hereinafter referred to as pointed nose) is sharp keen tip; Female member is provided with hole portion, and above-mentioned axial region just is inserted in the portion of hole after running through ear.This ear tag need use the erecting tools of regulation to be installed on the ear of every domestic animal, comes off to prevent ear tag.
When the workman handles the pig that ear tag has been installed, for preventing the workman, in patent documentation 1 and patent documentation 2, the design of above-mentioned ear tag is improved by head end institute scratch sharp keen on the male member, make on the axial region pointed nose can with shaft portion from.
In addition, disclosed domestic animal is with in the ear tag in patent documentation 3, demountable approximate cone shape sharp-pointed body (being equivalent to above-mentioned " pointed nose ") is installed on the male member, core bar (being equivalent to above-mentioned axial region) and base portion are made by hard resin, and only the flange part of blade of a sword shape is made by the rubber-like soft synthetic resin; Female member is also made by soft synthetic resin, and the core bar of male member is inserted on this female member movably.Because the flange part of this ear tag is very soft, so even collude extension mutually and be subjected to external force and do the time spent when ear tag and animal house, the ear of domestic animal can not bear very big burden yet.
Patent documentation 1: the spy opens clear 60-207534 number newspaper (with reference to claim 1 etc.)
Patent documentation 2: the spy opens flat 5-30874 number newspaper (with reference to claim 1 etc.)
Patent documentation 3: the spy opens flat 8-322415 number newspaper (with reference to claim 1 etc.)
But, need the problem that solves below in original technology, also existing.
In general, people are carried out to group feeding to pig to support in narrow space.Particularly as a rule, sow and piggy (about 10) are raised in same place (in the identical pigsty of identical pig house), up to piggy long to enough greatly till.In narrow pigsty, owing to piggy natural disposition is liked running foul of each other, quarrelling and fighting noisily, but also in pigsty, to run back and forth, ear happens occasionally with the situation that wall or pillar etc. colludes extension mutually.So, if the pointed nose of the male member of ear tag can with shaft portion from, then probably can come off, and drop on the ground of pigsty.Because the tip of pointed nose is very sharp-pointed, when it drops on the ground of pigsty, just has and make the injured danger of other pigs in raiser or the same pigsty.
In addition, after the ground ight soil of pigsty is collected, carry out processing procedures such as drying, compost, active sludge treatment.When the pointed nose of male member is sneaked in the ight soil of collection, will enter handling process with ight soil, thereby produce breakage science and engineering journey with machinery or make mechanical problems such as performance reduction.And, when the pointed nose of Damxung member is sneaked in the compost of handling again through above-mentioned flow process, can be to using the people of these compost, as the safety of managing the workman of the farmers' of cheese industry and other farmers' etc. constitutes a threat to.
In addition, if the pointed nose of male member can separate, when processing and manufacturing, can produce again can not ear tag is integrally formed problem.For example, when the above-mentioned patent documentation 3 middle ear targets hero of processing member, must make three assemblies such as sharp-pointed body, core bar and base portion, flange part respectively.Like this, because can not integrally formed male member, therefore be difficult to produce in enormous quantities at low cost the ear tag product.
And in the ear tag described in the patent documentation 3, though only the blade of a sword shape flange part of its male member is made by the rubber-like soft synthetic resin, its axial region is still by hard resin to be made.Therefore, when ear tag is subjected to external force and does the time spent because of colluding mutually to hang with animal house since axial region inadequately softness make the ear of domestic animal bear bigger burden, so this ear tag is unsuitable for being used on the body of the not only soft but also thin pig of ear.
And,, when utilizing ear tag that pig is carried out individual identification, must ear tag just be installed in the piggy stage because the growth of pig is very fast.Because the ear of piggy is not only soft but also thin, when colluding mutually, ear and wall hang or when contacting with other piggys, and be easy to tear and ear tag is come off.Therefore, the used ear tag of pig than other domestic animals use more soft, can also be securely fixed on the not only soft but also thin ear simultaneously.
Because the ear of piggy is very little, its ear tag is inevitable also very little.Therefore, if on ear tag, print literal that individual identification uses, numeral etc., then be difficult to identification probably because do not see.Thereby should satisfy such condition with ear tag as pig: though very little, but essential information also should come into plain view.
Summary of the invention
Therefore, main purpose of the present invention provides the special-purpose ear tag of the very little pig of a kind of burden that the pig ear is caused, and, even the very little also individual discrimination method of the pig of easy individual identification of ear tag
In order to solve above-mentioned technical task, adopted following scheme among the present invention.
At first, be made of first member and second member, wherein, above-mentioned first member is made by flexible synthetic resin, the axial region that has flat blade of a sword portion at least and give prominence to and be provided with in head-end location indissociable cone shape pointed nose from this blade of a sword portion; Above-mentioned second member has at least and is used for the above-mentioned hole portion of running through the axial region of domestic animal ear of plug-in mounting, and under above-mentioned plug-in mounting state, with the flat part of above-mentioned blade of a sword portion relative and common clamping domestic animal ear with it.
One of feature of this ear tag be pointed nose can not with shaft portion from.For example, can be by with the integrally formed method of resin the two can not being separated with axial region pointed nose, also the head end of this pig with the pointed nose of ear tag first member can be shaped separately with hard resin, and then the axial region that makes it to make with flexible synthetic resin by crimping, interlocking, welding, method such as bonding combines.And, when pointed nose and axial region are integrally formed with resin, can produce pig at low cost in enormous quantities and use the ear tag product.
By pointed nose can not be separated with axial region, can also get rid of owing to sharp-pointed pointed nose drops on the danger that makes the raiser injured at work in the pigsty, also avoided pointed nose to sneak in the fecaluria.If pointed nose has been sneaked in the ight soil, then can produce the problem of ight soil processing engineering of damaging with plant equipment.And because the ight soil of domestic animal utilizes with forms such as compost after treatment through being everlasting again, the pointed nose that therefore also will consider to sneak into compost spreads to the problem in the field.So,, just above problem can be solved in the lump if adopt the pointed nose of first member and the ear tag that axial region is connected as a single entity.
In addition, when this boar of installation is used ear tag on the pig ear, expose out for preventing sharp-pointed pointed nose, the pointed nose of first member can not project into the outside from the hole portion of second member.So just can guarantee the safety of raising work that the raiser is daily and animal doctor's vaccine inoculation work, also eliminate the danger that other pig is stabbed by sharp-pointed pointed nose in the same pigsty simultaneously.
Middle ear target first member of the present invention is made by flexible synthetic resin.Because blade of a sword portion and axial region are made by flexible synthetic resin, make the softness of ear tag integral body be improved.Therefore, even ear tag and animal house collude extension and be subjected to the external force effect, increase burden also can not for the not only soft but also thin ear of pig, thereby can not damage the pig ear.
Except that the first above-mentioned member, second member also can be made by flexible synthetic resin.Consequently the flat part of the blade of a sword portion of first member and second member all has softness.Because blade of a sword portion and flat part are all very soft, so ear tag can be securely fixed on the pig ear and closely contact with it, thereby can not injure the pig ear because of the ear tag swing.
Can indicate the lettering that individual identification is used this boar on the blade of a sword portion of ear tag and flat part, comprise the management number of individual identification number, birthplace, producer's (enterprise's name), farm name, groups of individuals etc.For example, by printing the management number of groups of individuals, can obtain to import the detailed recorded information of farm lane database at an easy rate.So-called detailed recorded information comprises the father and mother's (blood relationship chain), date of birth, raising state (the feed kind of being fed and the variation of body weight etc.) of the pig of investigating etc.
Secondly, when using this boar to use ear tag, can also make first member and second member be with look respectively, thus the method that provides a kind of combination that utilizes different colours that the domestic animal individuality is discerned.Like this, even ear tag is very little, also can come into plain view to basic identifying information.For example, by the simple combination of first member and the second member color, just can express the place of production of pig.In addition, can also identify more specifying information by on ear tag, printing characters such as identification number.
Here, when carrying out the domestic animal individual identification with this ear tag, first member of ear tag and second member also can be made with coloured synthetic resin.Promptly not to smear shades of colour on ear tag, but make with coloured synthetic resin in advance, so just do not worry that color comes off, can reduce manufacturing process yet the pig that makes.
Description of drawings
Fig. 1 is the stereoscopic figure of first member (A) of ear tag of the present invention
Fig. 2 is the vertical view during from the blade of a sword portion (3) of this first member (A) of back side unilateral observation
Fig. 3 is the sectional side view of an embodiment of this first member (A)
Fig. 4 is the stereoscopic figure of second member (B) of ear tag of the present invention
Fig. 5 is the vertical view during from the flat part 5 of back side unilateral observation second member (B)
Fig. 6 is the local section schematic diagram when being installed in ear tag on the pig ear (E)
Fig. 7 is the schematic appearance when being installed in ear tag on the pig ear (E)
Among the figure: 1-pointed nose, 2-axial region, 3-blade of a sword portion, 4-hole portion, 5-flat part, 6-end difference, 42-clamping part, A-first member, B-second member, E-pig ear.
Embodiment
Below, describe with reference to the example of accompanying drawing the preferred embodiments of the present invention.But the present invention is not limited to following embodiment.
Fig. 1 is the stereoscopic figure of pig of the present invention with the ear tag first member A; Fig. 2 is the vertical view of the bottom surface of the first member A.At first, the first member A is made up of the sharp-pointed approximate conical pointed nose 1 of head end, the discoideus blade of a sword portion 3 that supports the axial region 2 of this pointed nose 1 and be deployed in axial region 2 lower ends.
Pointed nose 1 can not separate with axial region 2.The effect of pointed nose 1 is to give pig mounting ear timestamp, perforate on ear, and run through ear.
Axial region 2 is cylindrical, and its diameter diminishes gradually along the direction towards top pointed nose 1.In addition, the inside of axial region 2 is hollow, makes axial region 2 have the flexibility of appropriateness.The height of axial region 2 is by factors such as the thickness decision of pig ear.
Pig should migrate to the stage of fattening in the circle in the piggy stage or from little pigsty with the installation of ear tag and carry out.In little pigsty, the piggy in together all ages is used as a big groups of individuals and raises together.Migrate to fatten circle after, before delivering for sale as pork pig, the pig of same groups of individuals is raised together in a narrow space.In same pigsty, strong pig can sting the ear tag of small and weak pig fall or bite into pieces.And when pig moved around in narrow pigsty, ear also had the danger of being colluded extension by fence or trough.
Therefore wish that ear tag can be securely fixed on the ear.For this reason, when the height of decision axial region 2, must take into full account this point.Specifically, the height of axial region 2 should be complementary with the thickness of mounting ear timestamp (piggy) ear.After the size design when installing according to ear tag, along with piggy enters fattening stage, the continuous thickening of ear, ear tag also just can be closely attached on the ear.Like this, because ear tag and ear fluid-tight engagement, just can prevent effectively that strong pig from biting ear tag into pieces or ear tag is colluded extension by pigsty fence etc. and situation about coming off.
Blade of a sword portion 3 is laminal flanges.It is discoideus that but the shape of blade of a sword portion 3 also is not limited to, and can also be other arbitrary shapes.The surface 31 of blade of a sword portion 3 is mounting ear timestamp and the contacted plane of pig ear.The back side 32 of blade of a sword portion 3 can be used to print characters such as identification number.Because blade of a sword portion 3 is to form by flexible synthetic resin is integrally formed with axial region 2, thereby have the elasticity and the softness of appropriateness.Be subjected to external force at ear tag and do the time spent, such structure just can alleviate the burden of pig ear.
In addition, the central portion in the bottom surface (back side 32 of blade of a sword portion) of the first member A is provided with a hole 21 (with reference to Fig. 2).Hole 21 is equivalent to the hollow bulb of axial region 2.Giving pig mounting ear timestamp, hole 21 is used to place mounting ear target " pliers ".
Fig. 3 is the cross section of the embodiment of the first member A.Head end at the pointed nose 1 of the first member A shown in Fig. 3 is to be made by hard resin.Because the intensity of this hard resin portion 13 far above the axial region of being made by soft synthetic resin 2 etc., therefore can make ear tag run through the ear of pig more like a cork.
When making the first member A, produce hard resin portion 13 at first separately, when the first member A that is shaped with soft synthetic resin etc., hard resin portion 13 and axial region 2 are fused by crimping, interlocking, welding or means such as bonding.According to such method, hard resin portion 13 will imbed in the soft synthetic resin part of pointed nose 1, thereby can not separate.In addition, can not separate with axial region 2 as long as can guarantee pointed nose 1, embodiments of the invention are not limited in that this is a kind of.
Simultaneously, the first member A makes by flexible synthetic resin is integrally formed.For example adoptable material has polypropylene, vinyl chloride, polyethylene, polyurethane, rubber etc.Even the integrally formed first member A, the thickness and the shape of pointed nose 1, axial region 2, blade of a sword portion 3 also can have nothing in common with each other, so pointed nose 1 is harder, and axial region 2 has the flexibility of appropriateness and is convenient to bending, and 3 in blade of a sword portion is quite soft.The first member A can also make with coloured synthetic resin is integrally formed.
Fig. 4 is the stereoscopic figure of the second member B, and Fig. 5 then is the vertical view of the bottom surface of second member.At first, the second member B is provided with to the hole cylindraceous of upper process portion 4, is deployed in the discoid flat part 5 of hole portion 4 lower ends, is located at the end difference 6 of the pedestal shape of hole portion 4 lower ends.
The inner hollow of hole portion 4.The inner cylinder portion 41 of hole portion 4 is positions of 1 plug-in mounting of pointed nose of the mounting ear timestamp first member A.Do not limit though the height of the cylinder of hole portion 4 is special, should guarantee mounting ear timestamp on pig ear E, the head end 11 of pointed nose 1 can not project into the outside from hole portion 4.Because sharp-pointed pointed nose 1 can not be exposed, it is hereby ensured the safety of raiser's daily raising work and animal doctor's vaccine inoculation work.Also eliminated simultaneously enter same pigsty other pig because of contact injured danger with pointed nose.
Laminal flat part 5 is parts that the mounting ear timestamp is positioned at blade of a sword portion 3 opposites of the first member A.At the mounting ear timestamp, the back side 52 of flat part 5 contacts with the pig ear.The surface 31 of the blade of a sword portion 3 of the first member A is relative with the back side 52 of the flat part 5 of the second member B, common clamping pig ear.On the surface 51 of flat part 5, can print characters such as identification number.The same with blade of a sword portion 3, flat part 5 also has the elasticity and the softness of appropriateness.Such design can be subjected to external force alleviates the pig ear as the time spent burden at ear tag.
End difference 6 is to be used for second member is installed in step portion on the regulation installation portion of " pliers " (not shown).So-called pliers is the instrument of a kind of similar cutting pliers (pinchers), and the mounting ear timestamp need be with the pliers of this special use on the pig ear.Pliers one side's installation portion inserts in the hole at the back side of the first member A, and the end difference 6 of the opposing party and second member is closely chimeric.Like this, by the pliers application of force is made its closure, just ear tag can be installed on the pig ear.Therefore, the shape of end difference 6, pattern must be complementary with size, the shape of the part that contacts with the second member B that ear tag is installed the pincers of usefulness.
Central portion in the bottom surface (back side 52 of flat part 5) of the second member B has hole portion 4 (Fig. 5).Be the back side 52 that the hole portion 4 of hollow is opened on the second member B.At the mounting ear timestamp, with the pointed nose 1 of the first member A from the back side 4 li in 52 1 side patchhole portions.
Though the second member B also can be made by hard resin, preferably also with first member A is the same is made by flexible synthetic resin.When the synthetic resin by flexibility forms the second member B, can adopt above-mentioned and the first member A identical materials.In addition, the second member B can also be integrally formed with coloured synthetic resin.
Fig. 6 is the local skiagraph when being installed in ear tag on the pig ear that is designated as E.As shown in Figure 6, the surface 31 of the blade of a sword portion 3 of the first member A is relative with the back side 52 of the flat part 5 of the second member B, common clamping pig ear E.The pointed nose 1 of the first member A is inserted in the hole portion 4 of the second member B after running through pig ear E.Be provided with in the lower end of the hole portion 4 of the second member B and be designated as 42 clamping part.This clamping part 42 is stepped, and to compare internal diameter littler with the inner cylinder portion 41 of hole portion 4.
Bottom surface sections 12 diameters of the pointed nose 1 of the first member A are greater than the inner cylinder portion diameter of the clamping part 42 of the second member B, but are slightly less than inner cylinder portion 41 diameters of hollows in the hole portion 4.Though the diameter of bottom surface sections 12 is greater than the inner cylinder portion diameter of clamping part 42, but because pointed nose 1 has the shape of approximate circle frustum, therefore when being imposed by force external force from the below, can pass clamping part 42 (inner cylinder portion) and enter among the inner cylinder portion 41 of hole portion 4.In case the pointed nose 1 of the first member A has entered inner cylinder portion 41, the bottom surface sections 12 of pointed nose 1 is owing to by 42 buckles of the clamping part of the second member B, just can't be pulled out.
Mounting ear timestamp on pig ear E wishes to guarantee that ear tag can be securely fixed on the pig ear E.Thereby just should decide the height (thickness) of the clamping part 42 of the length of axial region 2 of the first member A and the second member B according to the thickness of pig ear E.In addition, if clamping part 42 is too high or hole portion 4 is low excessively, the sharp-pointed cephalic par 11 of the first member A Tipping Center head 1 will project upwards from hole portion 4 and cause danger.
Fig. 7 is installed in schematic diagram on the pig ear E with ear tag.By the first member A and the second member B are with look respectively, just can utilize ear tag to carry out the individual identification of pig.
For example, when first member uses 7 kinds of colors, when second member also uses 7 kinds of colors, just can constitute 49 kinds of color combination.Therefore, by make up the place of production (as the title in Dou Daofu county) that defines pig with different colours, just can the information profile in the place of production of relevant pig be come into plain view.
In addition, also can adopt another kind of recognition methods, promptly represent to represent the place of production concrete in these places (title in Dou Daofu county) with the color of the second member B simultaneously in the big places of production (regional title) such as the Northeast, nine divisions of China in remote antiquity with the color of the first member A.In addition, about raising the various information on farm,, can after determining built-up sequence, be printed on above blade of a sword portion 3 and the flat part 5 etc. with literal or sign format as close pig numbering, pigsty numbering, date of birth etc.
(invention effect)
The main effect that the present invention brought into play is as follows.
By pig can not be separated with axial region with the pointed nose of first member of ear tag, can avoid pointed nose to drop on the ground of pigsty, thus the safety that has improved ear tag.In addition, blade of a sword portion and axial region are all used the flexible synthetic resin manufacturing, can increase the softness of ear tag, be subjected to external force at ear tag and do the time spent, can alleviate the suffered burden of pig ear.And, because first member of ear tag is one of the forming, so can produce in enormous quantities at low cost.
When the installation pig is used ear tag on the pig ear, the head end that can guarantee the pointed nose of first member can not project into the outside from the upper end of the hole portion of second member, because sharp-pointed pointed nose can not come out, so can guarantee the safety of raiser's daily raising work and animal doctor's vaccine inoculation work.Also eliminated simultaneously enter same pigsty other pig because of contact injured danger with pointed nose.
By ear tag first member and second member are with look respectively, just can come into plain view according to the customizing messages of different color combination to mounting ear target pig individuality.

Claims (6)

1. a boar is used ear tag, it is characterized in that:
Constitute by first member and second member,
Wherein, described first member is formed by flexible synthetic resin, and the axial region that has flat blade of a sword portion at least and give prominence to and be provided with in head-end location indissociable cone shape pointed nose from this blade of a sword portion;
Described second member has the hole portion that plug-in mounting runs through the described axial region of domestic animal ear that is used at least, and under described plug-in mounting state, relative with described blade of a sword portion and with the flat part of this blade of a sword portion common clamping domestic animal ear.
2. pig as claimed in claim 1 is used ear tag, it is characterized in that: except first member, described second member is also formed by flexible synthetic resin.
3. pig as claimed in claim 1 is used ear tag, it is characterized in that: described first member is integrally formed by resin.
4. pig as claimed in claim 1 is used ear tag, it is characterized in that: be marked with the lettering that individual identification is used in described blade of a sword portion or described flat part.
5. a domestic animal individual discrimination method is characterized in that: utilize the described pig of claim 1 to use ear tag, and make first member and the second member band look respectively, utilize its different color combination to carry out the individual identification of domestic animal.
6. domestic animal individual discrimination method as claimed in claim 5 is characterized in that: first member and second member are formed by colouring resin.
CN 200310123244 2003-05-29 2003-12-18 Ear signal for pig and livestock identifying method Pending CN1572138A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003153282 2003-05-29
JP2003153282A JP2004350598A (en) 2003-05-29 2003-05-29 Earmark for pig, and method for identifying livestock

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Publication Number Publication Date
CN1572138A true CN1572138A (en) 2005-02-02

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100357955C (en) * 2005-09-13 2007-12-26 上海农业信息有限公司 Pork supply system and method
CN112654242A (en) * 2018-08-28 2021-04-13 松下知识产权经营株式会社 Individual recognition member for animal and animal information management system
CN113367093A (en) * 2021-04-29 2021-09-10 福建省水产研究所(福建水产病害防治中心) Fixing method of fin-shaped spine sleeve for fish in-vitro marker

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
FR2879399B1 (en) * 2004-12-22 2007-02-16 Affflex Europ Soc Par Actions ANIMAL MARKING BUTTON HAVING A HULL COMPRISING TWO SHADED ELEMENTS, ONE TO BLACK CARBON AND THE OTHER COLOR LIVED
JP4830892B2 (en) * 2007-02-19 2011-12-07 オムロン株式会社 Fixture and non-contact IC tag
KR100969215B1 (en) 2007-12-18 2010-07-09 한국과학기술원 Marking method for the discrimination of turtles
JP5339148B2 (en) * 2009-08-26 2013-11-13 久雄 三浦 New livestock identification method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100357955C (en) * 2005-09-13 2007-12-26 上海农业信息有限公司 Pork supply system and method
CN112654242A (en) * 2018-08-28 2021-04-13 松下知识产权经营株式会社 Individual recognition member for animal and animal information management system
US11612144B2 (en) 2018-08-28 2023-03-28 Panasonic Intellectual Property Management Co., Ltd. Animal individual identification member and animal data management system
CN113367093A (en) * 2021-04-29 2021-09-10 福建省水产研究所(福建水产病害防治中心) Fixing method of fin-shaped spine sleeve for fish in-vitro marker

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