CN1570632A - Biochemical detection liquid for glucose in urine - Google Patents
Biochemical detection liquid for glucose in urine Download PDFInfo
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- CN1570632A CN1570632A CN 03141648 CN03141648A CN1570632A CN 1570632 A CN1570632 A CN 1570632A CN 03141648 CN03141648 CN 03141648 CN 03141648 A CN03141648 A CN 03141648A CN 1570632 A CN1570632 A CN 1570632A
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Abstract
This is a biochemistry detection liquid of urine glucose comprising 9.8-17.3w/v percents citric acid trisodium (sodium citrate) 5.3-100w/v percents anhydrous sodium carbonate, 1.3-1.73w/v percents blue copperas and distilled water. This invention offers biochemistry detection liquid and self-checking method of urine glucose for the mass scoutting and checking dynamically. Through the recording curve diagram in accordance with series detecting color result one can self-judge something: 1, warning signal of health and pathogenic factor; 2. forepart detectable rate of diabetes mellitus; 3, the synchronous tracking monitoring of curative effect during the treating course. The above liquid offers the likelihood of carrying out all this function.
Description
Technical field
The present invention relates to a kind of glucose in urine biochemistry detection liquid based on glucose in urine and uses thereof
Background technology
Diabetes are a kind of diseases occurred frequently, early stage clinically or do not have a classical symptom phase and often be difficult to find, be the chronic disease process, the patient often knows and once suffers from diabetes for many years after 15～20 years and because of having produced beginning of seeking medical advice just such as complication-cardiovascular and cerebrovascular diseases, hypopsia, hypercoagulable blood.It is the main foundation of examining that hospital inspection surpasses 160mg% kidney threshold value with blood sugar.
Owing to the detection of blood sugar tolerance, blood sugar test, refer to that the end measures detection a little, all need especially can not in the masses, generally carry out the regulation and control level of periodicity detection of dynamic repeatedly self insulin secretion function on the one in hospital with instrument detecting and interpretation.The chance of having ignored the diabetes early diagnosiss clinically because of the Static Detection value.But at present the detection technique of hospital and expensive detection equipment all are difficult in broad masses to promote the use of as the general inspection technology of the long-acting sub-health population of examination stably.
On the other hand, along with people for the concern of health and the raising constantly of conscious health care consciousness, hope can be beyond clinical such as ordinary conditions such as family, community, tourist sites under, can do self-dynamic tracking detection to Human Physiology or pathological condition, realize easy self-health monitoring, unhealthy early warning signal be provided, improve the early detective rate of chronic or latent diabetes, then to the synchronization monitoring of early treatment and curative effect process, to improve cure rate.
But up to now, for glycometabolic Function detection, Shang Weiyou crosses the method for suitable clinical outer " use among the people " and the relevant report of product.
Therefore, no matter be clinical diabetes curative effect monitoring or the oneself of family early prevention, all need a kind of quick, easy, glucose in urine biochemistry detection liquid and glucose in urine self-detection method accurately.The present invention has just satisfied this demand, provides possibility for realizing glycometabolic family oneself's tracking and monitoring and clinical preliminary examination.
Summary of the invention
Technical matters:
The invention provides a kind of mensuration glucose in urine biochemistry detection liquid
The present invention also provides the instructions of measuring the glucose in urine biochemistry detection liquid
The present invention also provides the gradual change of measuring the differential responses look that the glucose in urine variable concentrations shown to quantize chromatogram, for the tester carry out testing result contrast, and will detect color bits that the period shows by difference and be recorded in detection of dynamic outcome record figure and carry out the glucose in urine self-detection method and the judgement of glucose in urine testing result illustrates: " zero " health "? " not too healthy "+" is unhealthy.
Technical scheme:
The invention provides a kind of glucose in urine biochemistry detection liquid, it comprises 9.8-17.3w/v% trisodium citrate (sodium citrate), 5.3-100w/v% natrium carbonicum calcinatum, 1.3-1.73w/v% copper sulphate and distilled water.
The present invention also provides a kind of instructions that detects glucose in urine, and wherein content comprises glucose in urine self-detection method, urine ketoboidies biochemistry detection agent and urine ketoboidies self-detection method, test tube and glass sheet.
The prescription of described glucose in urine detectable is as follows
| Composition | Content |
| Trisodium citrate (sodium citrate) | 9.8-17.3 gram |
| Anhydrous acid sodium | 5.3-100 gram |
| Copper sulphate | 1.3-1.73 gram |
| Distilled water adds to | 100 milliliters |
Described instructions comprises:
The reaction that glucose in urine biochemistry detection liquid of preparing according to above-mentioned prescription and freshly voided urine sample produce exists if show the reducing substances based on glucose of contained variable concentrations in pale blue, bluish-green, yellowish green, yellowish-brown, reddish brown, the red all promptings of the firebrick urine.The positive test symbol of showed different.Indication: blood sugar surpasses the kidney threshold value of 160 milligrams of %, and the reflection of the terminal glucose in urine testing result of I type after heavily absorbing by glomerular filtration and renal tubule or type ii diabetes; Perhaps blood sugar surpasses the kidney threshold value of 160 milligrams of %, and the kidney glucose in urine terminal testing result reflection of renal tubule when heavily absorbing the glucose dysfunction.
According to the different colours that reaction presented that different glucose in urine concentration in glucose in urine biochemistry detection liquid and the freshly voided urine produce, provide gradual change to quantize chromatogram and contrasted use for the tester.
Difference according to the glycometabolic physiology of personalization, pathology regulation and control amplitude.Therefore the dynamic tracking that must carry out complete cycle when judging glycometabolic terminal situation detects, can be according to the glycometabolism physiological law with the result of continuous detection day part institute reaction solution for several times, fill in the record diagram that instructions provides, the result is linked to be curve, be depicted as dynamic curve diagram, just can obtain judging comparatively accurately from diagram: whether prompting is the synchronization monitoring of diabetes, diabetes spp curative effect in pathogenesis, raising diabetes early detective rate and the treating diabetes process of which kind of type, diabetes.
This instructions also provides a kind of tradition urine ketoboidies biochemistry detection agent that detects urine ketoboidies metabolic function.
| Composition | Content |
| Sodium nitroprusside | 1.24 gram |
| Natrium carbonicum calcinatum | 37.04 gram |
| Sulfuric acid amine | 61.73 gram |
| Total amount | 100 grams |
Behind classical maneuver dropping freshly voided urine sample, all point out the ketoboidies that contain different amounts in urine existence as if apparent pale red, aubergine, the demonstration positive test symbol indicates that diabetes have entered the severe stage.
The instructions of glucose in urine biochemistry detection liquid also comprises test tube, slide, so that the user uses.The amount of dripping of used drop bottle is 20 ± 2/ml, and " one " is equivalent to about 0.045-0.05ml.
The described sample freshly voided urine-urine specimen that has body temperature of promptly in time testing oneself promptly is fresh.
Beneficial effect
Compared with prior art, the invention provides quick, easy, glucose in urine biochemistry detection liquid and glucose in urine self-detection method accurately.Be the tracking dynamic monitoring of realization glycometabolism adjusting function, and of a mass character self-examination, the preliminary examination of diabetes control provide possibility.And, on criterion, realized good coincideing, thereby had very strong versatility and society popularizes meaning with the glucose in urine testing result of classics.
Embodiment
Embodiment 1
Glucose in urine detects the preparation of liquid
Technological requirement:
Trisodium citrate (sodium citrate) adds natrium carbonicum calcinatum earlier with behind the minor amount of water heating hydrotropy, adds copper sulphate again and is stirred to moltenly entirely, and adding distil water be to need joining total amount, send and tests if there is impurity need filter bottling.
Products obtained therefrom is blue solution, and PH is 10.7 ± 0.2, and the 430nm absorbance is that 0.15～0.32A preserves with white bottle sealing normal temperature.
Embodiment 2
The sensitivity of glucose in urine biochemistry detection liquid
Take by weighing 4 gram glucose dry powder, use the 100ml dissolved in distilled water, be prepared into the 4w/v% glucose solution.According to 2 times of serial dilutions, be prepared into 2w/v%, 1w/v%, 0.5w/v%, 0.25w/v%, 0.125w/v%, 0.0625w/v%, 0.03w/v%, the simulation glycosuria person urine of 0.015w/v%, and indicate concentration.
Each is drawn blue glucose in urine and detects liquid 0.5ml in 8 glass test tubees, drip 2w/v% again, 1w/v%, 0.5w/v%, 0.25w/v%, 0.125w/v%, 0.0625w/v%, 0.03w/v%, each 1 of 0.015w/v% simulation glycosuria person urine is put into 98-100 ℃ of boiling water bath immediately and was taken out in 5 minutes and observe in test tube:
| ??g％ | The detectable concentration scope | |||||||
| 2％ | 1％ | 0.5％ | 0.25％ | ??0.125％ | ??0.0625％ | ??0.03％ | ??0.015％ | |
| The reaction look | Reddish brown | Pale brown | Yellowish green | Light yellow green | Green | Emerald green | Bluish-green | Light blue |
More than experiment shows that the sensitivity that the present invention detects liquid can reach about 0.03-0.015%.
Embodiment 3
Glycometabolism detects
In test tube, drip blue glucose in urine biochemistry detection liquid 0.5ml, drip 1 of urine again, test tubes is put into 98-100 ℃ of boiling water bath took out observation in 5 minutes;
| ??g％ | The detectable concentration scope | |||||||
| ??2％ | ??1％ | ??0.5％ | ??0.25％ | ??0.125％ | ??0.0625％ | ??0.03％ | ??0.015％ | |
| The reaction look | Reddish brown | Pale brown | Yellowish green | Light yellow green | Green | Emerald green | Bluish-green | Light blue |
| Qualitative sign | ??++++ | ??+++ | ??++ | ??++ | ??+ | ??+ | ??± | ??± |
In full accord according to the concentration range that the suggested glucose in urine concentration range of above colour developing result and classical way detect.
Claims (5)
- A glucose in urine biochemistry detection liquid its comprise 9.8-17.3w/v% trisodium citrate (sodium citrate), 5.3-100w/v% natrium carbonicum calcinatum, 1.3-1.73w/v% copper sulphate and distilled water.
- 2. the description of glucose in urine biochemistry detection liquid comprises glucose in urine oneself detection technique, comprises that the described glucose in urine biochemistry detection liquid of claim 1 consumption is that the ratio of main urine is 10: 1 with containing the glucose reducing substances.Contain the glucose in urine biochemistry detection liquid and need react 5 minutes in 98 ℃ of-100 ℃ of boiling water baths with the test tubes of urine, take out and observe: be the firebrick redness, precipitation is arranged, glucose in urine content is then greater than 2%; Being rufous has precipitation, and glucose in urine concentration is about 2%; Being yellow green has precipitation, and glucose in urine concentration is about 1%; Being green has small amount of precipitate, and glucose in urine concentration is about 0.5%; Being blue-green has the minute quantity precipitation, and glucose in urine concentration is about 0.25%; Pale blue is green does not have a precipitation, glucose in urine concentration be about 0.1%; Light blue is less than 0.1%.
- 3. description according to claim 2 comprises that also gradual change quantizes chromatogram; The different continuously performance graph record diagrams that detect the detection colour developing result of period.
- 4. content according to claim 2 also comprises glucose in urine self-detection method, urine ketoboidies detection agent and urine ketoboidies self-detection method, test tube and slide, moulds bottle its amount of groove clip is 20 ± 2 droplets/milliliter.
- 5. the purposes that is used for the qualitative determination of glucose in urine chromogenic reaction according to the described glucose in urine biochemistry detection liquid of claim 1.Mainly can carry out in the family one day repeatedly the oneself follow the tracks of detection of dynamic, show personalized glucose in urine metabolism dynamic regulation function whether balance zero, negative balance?, unbalance+early warning signal and probe into virulence factor; Improve the diabetes early detective rate, and the synchronization monitoring of diabetic's curative effect in therapeutic process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03141648 CN1570632A (en) | 2003-07-17 | 2003-07-17 | Biochemical detection liquid for glucose in urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03141648 CN1570632A (en) | 2003-07-17 | 2003-07-17 | Biochemical detection liquid for glucose in urine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1570632A true CN1570632A (en) | 2005-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03141648 Pending CN1570632A (en) | 2003-07-17 | 2003-07-17 | Biochemical detection liquid for glucose in urine |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102749327A (en) * | 2012-07-25 | 2012-10-24 | 中国科学院上海有机化学研究所 | Urine sugar rapid detecting method based on chemical chromogenic reaction at room temperature |
| TWI729663B (en) * | 2020-01-06 | 2021-06-01 | 財團法人亞洲大學 | Fast urine analyzing gel |
-
2003
- 2003-07-17 CN CN 03141648 patent/CN1570632A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102749327A (en) * | 2012-07-25 | 2012-10-24 | 中国科学院上海有机化学研究所 | Urine sugar rapid detecting method based on chemical chromogenic reaction at room temperature |
| TWI729663B (en) * | 2020-01-06 | 2021-06-01 | 財團法人亞洲大學 | Fast urine analyzing gel |
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