CN1570635A - Biochemical detection liquid for calcium in urine - Google Patents
Biochemical detection liquid for calcium in urine Download PDFInfo
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- CN1570635A CN1570635A CN 03141652 CN03141652A CN1570635A CN 1570635 A CN1570635 A CN 1570635A CN 03141652 CN03141652 CN 03141652 CN 03141652 A CN03141652 A CN 03141652A CN 1570635 A CN1570635 A CN 1570635A
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Abstract
This is a biochemistry detection liquid of urine calcium comprising 1.5-2w/v percents oxalic acid, 1.5-2w/v percents oxalic acid amine, 3.2- 3.5v/v percents glacial acetic acid and distilled water. This invention offers biochemistry detection liquid and self-checking method of urine calcium for the mass scoutting and checking dynamically. Through the recording curve diagram in accordance with series detecting color result one can self-judge something: 1, warning signal of health and pathogenic factor; 2, forepart detectable rate of calcium metabolism overbalancing; 3, the synchronous tracking monitoring of curative effect during the treating course. The above liquid offers the likelihood of carrying out all this function.
Description
Technical field
The present invention relates to a kind of urine calcium biochemistry detection liquid and uses thereof based on urine calcium
Background technology
1 kilogram of human body calcic total amount, 1.5% of percentage of liveweight, wherein 99% in bone calcium, when the X line finds have bone density to reduce decalcification more than 30%.
About 10 gram (1% is free in blood and the extracellular fluid) plasma calciums of calcic are less than 0.3 gram in the human body fluid, and serum total calcium concentration is 9-11mg%, accounts for 35% of the total calcium of human body.
Serum calcium has three kinds of form calcium: 1. in conjunction with calcium 2. non-binding calcium 3. ionized calciums.Ionized calcium has direct physiological action, participates in the physiological regulation reaction, accounts for 53% of TC.
The calcium physiological metabolism: calcium storehouse and blood calcium have the maintenance mobile equilibrium from steady system.Bone calcium surface has calcium 428 grams approximately, and frequent and plasma calcium exchanges.
From steady system do as one likes hormone, parathormone participates in calcium element, cholecalciferol etc. in regulation and control.Intestinal absorption calcium is more than the 20%-40%.Discharging from bone bank during hypocalcia keeps blood calcium concentration to keep whole physiological function.
CaE: when discharging the calcium amount in the every day urine according to what of intake, about about 100-200mg/day.Calcium content about 0.5 gram every day in the excrement.Just calcium balance can be kept so take the photograph calcium 0.8-1.0 gram every day, general physiological requirements can be kept.The urine calcium concentration that serum calcium drops to 8-4mg% significantly reduces or disappearance.
Calcium metabolism is unbalance to be a kind of common disease, early stage clinically or do not have a classical symptom phase and often be difficult to find that be the chronic disease process, carry out urine detection this moment can be from healthy population, and screening goes out to have the inferior health colony of suffering from the calcium deficiency tendency.The unbalance early detection of calcium metabolism is very easily effected a radical cure.
The chance of having ignored the unbalance early diagnosis treatment of calcium metabolism clinically because of the Static Detection value.Hospital also is difficult to generally carry out periodicity dynamic tracking detection repeatedly on the one in the masses at present.
On the other hand, along with people for the concern of health and improving constantly of self consciousness, hope can be beyond clinical such as ordinary conditions such as family, community, tourist sites under to the dynamic tracking monitoring of Human Physiology or pathological condition with detect, realization can improve easy oneself's screening of this disease early detective rate.Like this, can provide early warning signal, improve the unbalance early detective rate of calcium metabolism, improve early treatment and cure rate.But up to now, for the detection of calcium metabolism, Shang Weiyou crosses the method for suitable clinical outer " use among the people " and the relevant report of product.
Therefore, no matter be clinical or the monitoring of family self-prevention, need a kind of quick, easy, whether unbalance urine calcium biochemistry detection liquid and the urine calcium self-detection method of calcium metabolism accurately.The present invention has just satisfied this demand, for family's self-prevention monitoring and the clinical preliminary examination that realizes calcium metabolism provides possibility.
Summary of the invention
Technical matters:
The invention provides a kind of mensuration urine calcium biochemistry detection liquid
The present invention also provides the instructions of measuring urine calcium biochemistry detection liquid
The present invention also provide measure urine calcium urinate qualitatively gradual change that the calcium biochemistry detection liquid is used to detect the qualitative colour developing of urine calcium in preparation quantize the chromatogram explanation for the tester contrast, continuously different period results' detection of dynamic outcome record figure and urine calcium self-detection method and testing result judge diagram: " zero " health "? " not too healthy "+" is unhealthy.
Technical scheme:
The invention provides a kind of urine calcium and urinate the calcium biochemistry detection liquid qualitatively, it comprises 1.5-2w/v% oxalic acid, 1.5-2w/v% oxamide, 3.2-3.5v/v% glacial acetic acid and distilled water.
The prescription of described urine calcium detectable is as follows
| Composition | Content |
| Oxalic acid | 1.5-2 gram |
| Oxamide | 1.5-2 gram |
| Glacial acetic acid | 3.2-3.5 milliliter |
| Distilled water adds to | 100 milliliters |
Described instructions comprises:
The reaction that urine calcium biochemistry detection liquid of preparing according to above-mentioned prescription and freshly voided urine produce is if show milky white precipitate, white microprecipitation, white opacity, shallow muddy, micro-muddy, little muddy, positive test symbol that vestige shows 0.2%, 0.1%, 0.05%, 0.025%, 0.012%, 0.006%, 0.003% different urine calcium concentrations.
React according to contained calcium concentration in urine calcium biochemistry detection liquid and the freshly voided urine and to present different colors, provide the gradual change quantification chromatogram of urine calcium variable concentrations to illustrate and contrasted use for the tester.
According to the physiology of personalization urine calcium metabolism, the difference of pathology regulation and control amplitude.Therefore the dynamic tracking that must carry out complete cycle when judging the terminal situation of calcium metabolism detects, can be with continuous testing result for several times according to the time period of calcium metabolism physiological law and the result of day part reaction solution, fill in the record diagram that instructions provides, then the result is linked to be curve, be depicted as urine calcium and detect dynamically recording figure,, can obtain judging comparatively accurately from curve diagram: whether be calcium metabolism balance, and unbalance reason.Improve the unbalance early detective rate of calcium metabolism, and to curative effect synchronization monitoring in the therapeutic process of taking the photograph hypocalcia and endocrine disturbance person.
Described freshly voided urine-the urine specimen that has body temperature of in time testing oneself immediately is fresh.
This instructions also provides the uric acid alkali biochemistry detection liquid of traditional classical, and its prescription is as follows
| Composition | Content |
| Phenol red (phenol red) | 0.01 gram |
| Distilled water adds to | 100 milliliters |
Uric acid alkali biochemistry detection liquid is 1: 1 with the ratio of freshly voided urine detection, can be to react on the slide.Alkaline urine takes on a red color, and neutral urine is orange, and it is yellow that aciduria is.And provide uric acid alkali biochemistry detection liquid and freshly voided urine reaction solution result's gradual change quantification chromatogram to contrast use for the tester.Prompting differentiates that acid urine forms the disease of crystallization and alkaline urine formation crystallization.
The present invention also provides a kind of detection urine calcium to urinate calcium biochemistry detection liquid instructions qualitatively, comprising urinating calcium oneself detection method, uric acid alkali biochemistry detection liquid, reaching urine calcium self-detection method, test tube, slide.The amount of dripping of used dropper is 20 ± 2/ml, and " one " is equivalent to about 0.045-0.05ml.
Beneficial effect
Compared with prior art, the invention provides quick, easy, urinate calcium biochemistry detection liquid and urine calcium self-detection method accurately.Be the self-monitoring of realization urine calcium function, and the unbalance clinical preceding preliminary examination of calcium metabolism provide possibility.Realize good coincideing with the urine calcium testing result of classics, thereby had the universal meaning of very strong versatility and society.
Embodiment
Embodiment 1
The preparation of urine calcium biochemistry detection liquid
Technological requirement:
Oxalic acid adds oxamide and adds glacial acetic acid and be blended in and be stirred to moltenly entirely greater than thermosol in 60 ℃ of containers, and last adding distil water is to needing dosage.。
Products obtained therefrom is colourless transparent solution, and PH is 2.25 ± 0.25, preserves with white bottle lucifuge sealing normal temperature.
Embodiment 2
The sensitivity of urine calcium biochemistry detection liquid
Getting calcium carbonate aqueous solution, to make concentration be that 0.2%, 0.1%, 0.05%, 0.025%, 0.012%, 0.006%, 0.003% capacity is in 7 test tubes of 0.5ml, and indicate concentration.
In above-mentioned 7 test tubes, observe after the urine calcium biochemistry detection liquid of dropping equivalent:
| The detectable concentration scope | |||||||
| ??g％ | ??0.2 | ??0.1 | ??0.05 | ??0.025 | ??0.012 | ??0.006 | ??0.003 |
| The reaction look | Milky white precipitate | White microprecipitation | White opacity | Shallow muddy | Micro-muddy | Little muddy | Vestige |
| Clinical judgment | ??++++ | ??+++ | ??++ | ??+ | ??+ | ??- | ??- |
More than experiment shows that the sensitivity of this urine calcium biochemistry detection liquid can reach about 0.01-0.003%.
Embodiment 3
Calcium metabolism detects
What drip that 24 hours (12 hours * 2) always urinate total volume of urine shakes up 10 in urine, adds equivalent urine calcium biochemistry detection liquid and observes: if show milky white precipitate, white microprecipitation, white opacity, shallow muddy, micro-muddy, little muddy, testing results that vestige shows 0.2%, 0.1%, 0.05%, 0.025%, 0.012%, 0.006%, 0.003% different urine calcium concentrations.Do background with literal paper after test tubes: the clear urine calcium that is enlarged into of background literal reduces (0.006-0.001%); The background literal blurs but can distinguish that reading is urine calcium normal (0.025-0.012%); Fuzzy can not the debating of background literal reads to increase (0.2-0.05%) for urine calcium.
Comparatively identical according to above urine calcium colour developing result with classical urine calcium assay.
Claims (5)
- A urine calcium biochemistry detection liquid its comprise 1.5-2w/v% oxalic acid, 1.5-2w/v% oxamide, 3.2-3.5v/v% glacial acetic acid and distilled water.
- 2. the content of urine calcium biochemistry detection liquid instructions comprises that the ratio of the described urine calcium of calcic urine and claim 1 biochemistry detection liquid consumption is 10: 10 (1: 1).Sheng is urinated the calcium biochemistry detection liquid and is observed with the detection reaction of urine: if show milky white precipitate, white microprecipitation, white opacity, shallow muddy, micro-muddy, little muddy, vestige demonstration 0.2%, 0.1%, 0.05%, 0.025%, 0.012%, 0.006%, 0.003% different testing results of urinating calcium concentrations.
- 3. also comprise according to the described description of claim 2: gradual change quantizes the chromatogram explanation; The different continuously performance graph record diagrams that detect period detection colour developing result.
- 4. also comprise uric acid alkali biochemistry detection liquid and uric acid alkali oneself detection method, test tube, slide according to the described description of claim 2, moulding bottle its amount of groove clip is 20 ± 2 droplets/milliliter.
- 5. be used to urinate the purposes of the qualitative determination of calcium chromogenic reaction according to the described urine calcium of claim 1 biochemistry detection liquid.Mainly can carry out in the family one day repeatedly the oneself follow the tracks of detection of dynamic, show personalized urine calcium metabolism dynamic regulation function whether balance zero, negative balance?, unbalance+early warning signal and probe into virulence factor; Improve the unbalance early detective rate of urine calcium metabolism, and the curative effect synchronization monitoring of therapeutic process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03141652 CN1570635A (en) | 2003-07-17 | 2003-07-17 | Biochemical detection liquid for calcium in urine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03141652 CN1570635A (en) | 2003-07-17 | 2003-07-17 | Biochemical detection liquid for calcium in urine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1570635A true CN1570635A (en) | 2005-01-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03141652 Pending CN1570635A (en) | 2003-07-17 | 2003-07-17 | Biochemical detection liquid for calcium in urine |
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| CN (1) | CN1570635A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100489503C (en) * | 2005-10-28 | 2009-05-20 | 中国科学院长春应用化学研究所 | Method for measuring plasma calcium by triplex pyridine ruthenium electrochemical luminescence |
| CN106770265A (en) * | 2017-03-08 | 2017-05-31 | 山东省交通医院 | A kind of kit of quick detection urinary calcium |
| JP2020514716A (en) * | 2016-12-27 | 2020-05-21 | ヒルズ・ペット・ニュートリシャン・インコーポレーテッド | Calcium oxalate titration test kit and pet food composition |
-
2003
- 2003-07-17 CN CN 03141652 patent/CN1570635A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100489503C (en) * | 2005-10-28 | 2009-05-20 | 中国科学院长春应用化学研究所 | Method for measuring plasma calcium by triplex pyridine ruthenium electrochemical luminescence |
| JP2020514716A (en) * | 2016-12-27 | 2020-05-21 | ヒルズ・ペット・ニュートリシャン・インコーポレーテッド | Calcium oxalate titration test kit and pet food composition |
| US11330830B2 (en) | 2016-12-27 | 2022-05-17 | Hills Pet Nutrition, Inc. | Calcium oxalate titration test kits and pet food compositions |
| CN106770265A (en) * | 2017-03-08 | 2017-05-31 | 山东省交通医院 | A kind of kit of quick detection urinary calcium |
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