CN102749327A - Urine sugar rapid detecting method based on chemical chromogenic reaction at room temperature - Google Patents
Urine sugar rapid detecting method based on chemical chromogenic reaction at room temperature Download PDFInfo
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- CN102749327A CN102749327A CN2012102600063A CN201210260006A CN102749327A CN 102749327 A CN102749327 A CN 102749327A CN 2012102600063 A CN2012102600063 A CN 2012102600063A CN 201210260006 A CN201210260006 A CN 201210260006A CN 102749327 A CN102749327 A CN 102749327A
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Abstract
The invention provides an urine sugar rapid detecting method based on chemical chromogenic reaction at a room temperature. The method comprises the step of: through the adoption of the chemical chromogenic reaction after 4-amino-3-hydrazine-5-mercapto-1,2,4-triazole and periodate are oxidized with glucose in urine, determining substantial content of the glucose in the urine according to relevance between reaction solution color and glucose concentration.
Description
Technical field
Based on the glucose in urine fast detecting liquid of chemical colour reaction reaction, comprise A liquid, B liquid and deionized water under a kind of room temperature.Wherein, A liquid comprises 40mmol/L NaIO
4, 2mol/L KOH, solvent are deionized water; B liquid comprises 40mmol/L AHMT, and 0.12mol/L HCl, solvent are deionized water.The invention provides a kind of method of simple and easy to do detection glucose in urine content.Confirm the roughly content of glucose in the glucose in urine according to the difference of reactant liquor color.Still can effectively detect during at concentration of glucose, can effectively avoid false-negative generation at 1mmol/L.
Technical background
Diabetes are one of modal chronic diseases.The Along with people's growth in the living standard, the increase of aging population and incidence of obesity, the incidence of disease of diabetes is ascendant trend year by year.Diabetes reach 2% at the incidence of disease of China, and according to statistics, the diabetic that made a definite diagnosis of China reaches 4,000 ten thousand, and with annual 1000000 speed increase.Clinical early stage or do not have a classical symptom phase and often be difficult to find that be the chronic disease process, the patient is often behind 15-20 and because of having produced concurrent---brain cardiovascular disease, hypopsia, the just beginning knowledge of seeking medical advice such as high sticky blood has been suffered from diabetes for many years.It is the main foundation of examining that hospital inspection surpasses 160mg% kidney threshold value with blood sugar.
The method of traditional detection blood sugar detects blood sugar test like the blood sugar tolerance; Refer to the tip trace detection, all need blood extracting assay, sense cycle is long; Cause wound and pain to human body; Increase to infect and the routes of infection, and must be in hospital with instrument detecting and interpretation, its detection technique and expensive detection equipment all are difficult in broad masses as the general inspection of the examination sub-health population of long-term stability technological and promote the use of.More existing electronics blood-sugar detecting instruments still belong to the category that has wound to detect, and some directly uses blood glucose sensor that the sugar content in the saliva is detected, and the electrical signal conversion that records is become digital signal; Analyze, handle through computing machine; The content that directly shows blood sugar is though this blood glucose meter can draw correlated results, complex equipments fast; Cost is higher, is not suitable for family or the personnel's of family practice use.
Glucose in urine detects the two kinds of methods that mainly contain: the Benedict method, and the method is used for a long time, but because of method of operating is loaded down with trivial details, and the user can be by weak points such as burn or scalds sometimes, and the people who uses now is fewer and feweri.Usually adopt glucose in urine to detect test paper for the screening family of prevention usefulness and the method for the glucose content in the human urine, at present domestic existing many kinds of Tes-Tapes are sold, and this type test paper majority is the color reaction that on the zymochemistry basis, produces the glucose compliance.Its assay method is similar: the container that fills urine is put into Tes-Tape earlier in (1); (2) take out at once, stay a minute; (3) attempted colorimetric with interior with the different glucoses in urine that test paper is wrapped at 30 seconds, to confirm the content of glucose in urine; (4) result representes with "+".Also there is weak point in the Tes-Tape method: some medicine with reductibility also can make the Tes-Tape variable color, causes the high illusion of glucose in urine, like vitamin C, and salicylate etc.; The half-quantitative detection that can only be used for glucose in urine, and remolding sensitivity is lower, generally all reaches 50mg/dL at glucose in urine content, even 100mg/dL could show positive.2 yuan of the market prices of Tes-Tape/inferior, still higher for area underdeveloped.And the test paper of traditional glucolase is owing to the influence of operating ambient temperature, and precision is not too high, and is difficult for storing, and is prone to oxidation takes place in air, causes the inefficacy of test paper.
Summary of the invention
Technical matters:
The invention provides a kind of glucose chromogenic reagent.
The invention provides the glucose in urine method for quick that reacts based on chemical colour reaction under the cover room temperature and detect formula of liquid.
The invention provides the instructions of glucose in urine fast detecting liquid.
The present invention also provides and has measured the different colours deck watch that the glucose in urine variable concentrations is shown, supplies the tester to carry out the contrast of testing result, detects to carry out the glucose in urine oneself.
Technical scheme:
Glucose chromogenic reagent used in the present invention, chemistry 4-amino by name-3-hydrazine-5-dredges base-1,2, the 4-triazole (the Chinese another name: 4-amino-3-diazanyl-5-sulfydryl-1,2, the 4-triazole is abbreviated as AHMT), its structural formula is following:
Based on the glucose in urine method for quick of chemical colour reaction reaction, to be 7 to 14 alkaline solution fragment into dialdehyde with the adjacent hydroxyl oxidize of glucose in the urine to the pH scope of using periodate or acid iodide earlier under the room temperature of the present invention; In above-mentioned system, add the acid solution reaction of AHMT, product further is oxidized to the purple compound by excessive slightly oxygenant; According to the colour developing degree of depth and colorimetric card comparison, glucose is carried out semi-quantitative analysis;
The molar ratio range of described periodate or acid iodide and AHMT is 1:100-100:1;
Described AHMT is that 4-amino-3-hydrazine-5-dredges base-1,2,4-triazole.
The pH scope of described excess of periodate slightly or acid iodide is 7 to 14 alkaline solution, is NaIO
4Perhaps KIO
4The volumetric molar concentration scope be 1mmol/L-10mol/L, to regulate pH by potassium hydroxide, NaOH, supercarbonate, phosphate, borate or ammonium chloride-WS be 7 to 14 alkaline solution.
The acid solution of described AHMT be AHMT be 1mmol/L – 10mol/L, by the solution of hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, acetate, trifluoroacetic acid or other acidic buffer adjustment of acidity.
The pH scope of described excess of periodate slightly or acid iodide is that the volumetric molar concentration of NaOH or KOH is recommended as 2mol/L in 7 to 14 the alkaline solution.
In the acid solution of described AHMT, the volumetric molar concentration of AHMT is recommended as 40mmol/L.
Adopt the glucose in urine method for quick that reacts based on chemical colour reaction under the room temperature of the present invention, step is to get the urine that a volume spends 10 times of ionized water dilutions earlier; With equal-volume slightly the pH scope of excess of periodate or acid iodide be 7 to 14 alkaline solution vibration 30s; Place 10min, add the acid solution of equal-volume AHMT, behind the vibration 30s; With the standard color comparison card comparison, confirm the content of glucose in urine.
In other words, the invention provides the glucose in urine fast detecting liquid that reacts based on chemical colour reaction under a kind of room temperature, comprise A liquid, B liquid and deionized water.Wherein, A liquid comprises 40mmol/L NaIO
4, 2mol/L KOH, solvent are deionized water; B liquid comprises 40mmol/L AHMT, and 0.12mol/L HCl, solvent are deionized water.
The present invention also provides the instructions that detects glucose in urine, has wherein introduced the method for application of glucose in urine fast detecting liquid.
The prescription of said glucose in urine detectable is as follows:
It is some that the 0.6mL centrifuge tube is housed in the packing, is used for the dilution and the reaction vessel of urine.And be furnished with three glue head droppers, be used for the absorption of reactant liquor and the dilution of urine.
B liquid is preserved at the shading shady place, can deposit half a year.
Described instructions comprises:
According to the reaction that glucose in urine fast detecting liquid that above-mentioned prescription disposed and freshly voided urine sample take place, present the depth of purple, the content of the glucose of judging in the urine to be contained.
In the conventional detection method, the glucose in urine positive can be divided into trace, 1-4 plus siges, plus sige more multilist show glucose in urine content high more, because different relevant urinary glucose determination results' method for expressing is arranged, table 5 provides the relation between plus sige and the glucose in urine concentration.
The relation of plus sige and glucose in urine concentration
For this method, purple is following according to the pairing glucose in urine concentration of the depth:
(the glucose original liquid concentration that from left to right dilutes before 10 times is followed successively by 10mmol/L, 20mmol/L, 30mmol/L; 40mmol/L, 50mmol/L, 70mmol/L); As can be seen from the figure, glucose content can be divided into five rank: 10mmol/L, 20mmol/L; 30mmol/L, 40-50mmol/L, 70mmol/L.The variable color gradient is suitable with the routine inspection method.If the reactant liquor color drops between two concentration, then get intermediate value.If concentration is greater than 70mmol/L, then color can become darker.If glucose stoste dilution back colour developing is more shallow than 10mmol/L, explaining has micro-glucose to exist in the urine, then need not dilution, stoste is directly dropped into reaction can judge glucose content.As if colourless, then be expressed as normal person's urine.
Description of drawings
Fig. 1 .10,20,30,40,50, color developing effect figure (embodiment 2) after the D/W of 70mmol/L dilutes 10 times;
Fig. 2. it is 0,1,2,3,4,5 that normal person's urine dilution standard is added into concentration of glucose, reaction back color (embodiment 3) during 7mmol/L.
Embodiment
Embodiment 1
Glucose in urine detects the preparation of liquid
Technological requirement:
The preparation of A liquid: the KOH of 1.12g is dissolved in the deionized water of 10mL, heat release in the process, to be cooled to room temperature, to the NaIO that wherein adds 0.086g
4, dissolving, products obtained therefrom is colourless, is stored in sealing normal temperature preservation in the white plastic bottle.
The preparation of B liquid: the dense HCl of 0.2mL is added in the deionized water of 2.2mL, and after mixing, to the AHMT that wherein adds 0.058g, vibration adds deionized water to 10mL after fully dissolving.Be stored in sealing normal temperature preservation in the brown reagent bottle.
Embodiment 2
The sensitivity of Glucose Liquid monitoring
Take by weighing the glucose dry powder of 0.18g, use the 10mL deionized water dissolving, be prepared into the glucose solution of 100mmol/L, it is diluted to 1mmol/L, 2mmol/L, 3mmol/L, 4mmol/L, 5mmol/L, 7mmol/L respectively with deionized water.Simulation glycosuria person urine, and indicate concentration.
In the centrifuge tube of 0.6mL, each adds 50 μ L (glue head dropper then is, down together) sugar juice, and to wherein adding A liquid 50 μ L.After vibration 30s is even, place 10min,, compare with colorimetric card behind the vibration 30s to the B liquid that wherein adds 50 μ L.
For this method, purple is following according to the pairing sugared concentration of the depth:
From left to right the glucose original liquid concentration before the dilution is followed successively by 10mmol/L, 20mmol/L, 30mmol/L; 40mmol/L, 50mmol/L, 70mmol/L); As can be seen from the figure, glucose content can be divided into five rank: 10mmol/L, 20mmol/L; 30mmol/L, 40-50mmol/L, 70mmol/L.The variable color gradient is suitable with the routine inspection method.See Fig. 1.(blank sheet of paper can be filled up in the bottom during observation, reduces aberration.）
Embodiment 3
Glycosuria detects
In the 0.6mL centrifuge tube, add respectively with deionized water and diluted 9 times of 45 μ L normal person urines afterwards; And be respectively the D/W of 10mmol/L, 20mmol/L, 30mmol/L, 40mmol/L, 50mmol/L, 70mmol/L to the concentration that wherein adds 5 μ L respectively, being made into concentration gradient is 1mmol/L, 2mmol/L; 3mmol/L; 4mmol/L, 5mmol/L, the glucose in urine solution of 7mmol/L.In order to simulation glycosuria person urine, and indicate concentration (original glucose content is not calculated interior in the urine).
In the centrifuge tube of the 0.6mL that is placed with 50 μ L dilution back glucose in urine solution, each adds A liquid 50 μ L.After vibration 30s is even, place 10min,, compare with colorimetric card behind the vibration 30s to the B liquid that wherein adds 50 μ L.
For this method, purple can be divided into five scope: 10mmol/L, 20mmol/L, 30mmol/L, 40-50mmol/L, 70mmol/L according to the pairing sugared concentration of the depth.Consistent with the D/W color developing effect of same concentrations gradient.See Fig. 2.(blank sheet of paper can be filled up in the bottom during observation, reduces aberration.）。
Claims (7)
- Under the room temperature based on the glucose in urine method for quick of chemical colour reaction reaction, to it is characterized in that using earlier the excessive slightly pH scope that contains periodate or acid iodide be 7 to 14 alkaline solution fragments into dialdehyde with the adjacent hydroxyl oxidize of glucose in the urine; In above-mentioned system, add the acid solution reaction of AHMT, product further is oxidized to the purple compound by excessive slightly oxygenant; According to the colour developing degree of depth and colorimetric card comparison, glucose is carried out semi-quantitative analysis;The molar ratio range of described periodate or acid iodide and AHMT is 1:100-100:1;Described AHMT is that 4-amino-3-hydrazine-5-dredges base-1,2,4-triazole.
- 2. based on the glucose in urine method for quick of chemical colour reaction reaction, the pH scope that it is characterized in that described excess of periodate slightly or acid iodide is 7 to 14 alkaline solution, is NaIO under the room temperature as claimed in claim 1 4Perhaps KIO 4The volumetric molar concentration scope be 1mmol/L-10mol/L, to regulate pH by potassium hydroxide, NaOH, supercarbonate, phosphate, borate or ammonium chloride-WS be 7 to 14 alkaline solution.
- 3. under the room temperature as claimed in claim 1 based on the glucose in urine method for quick of chemical colour reaction reaction, the acid solution that it is characterized in that described AHMT be AHMT be 1mmol/L – 10mol/L, by the solution of hydrochloric acid, phosphoric acid, sulfuric acid, nitric acid, acetate, trifluoroacetic acid or other acidic buffer adjustment of acidity.
- Under according to claim 1 or claim 2 the room temperature based on the glucose in urine method for quick of chemical colour reaction reaction, the pH scope that it is characterized in that described excess of periodate slightly or acid iodide is that the volumetric molar concentration of NaOH or KOH is 2mol/L in 7 to 14 the alkaline solution.
- 5. the glucose in urine method for quick as reacting based on chemical colour reaction under claim 1 or the 3 described room temperatures is characterized in that in the acid solution of described AHMT that the volumetric molar concentration of AHMT is 40mmol/L.
- 6. based on the glucose in urine method for quick of chemical colour reaction reaction, it is characterized in that diluting 10 times with deionized water before the described urine detection under the room temperature as claimed in claim 1; During reaction, the volume of urine consumption after diluting 10 times and the pH scope of glucose in urine fast detecting liquid periodate or acid iodide are that the ratio of volumetric usage of the acid solution of 7 to 14 alkaline solution and AHMT is 1:1:1.
- 7. based on the glucose in urine method for quick of chemical colour reaction reaction, it is characterized in that described step does under the room temperature as claimed in claim 1, get the urine that a volume spends 10 times of ionized water dilutions earlier; With equal-volume slightly the pH scope of excess of periodate or acid iodide be 7 to 14 alkaline solution vibration 30s; Place 10min, add the acid solution of equal-volume AHMT, behind the vibration 30s; With the standard color comparison card comparison, confirm the content of glucose in urine.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016074282A1 (en) * | 2014-11-11 | 2016-05-19 | 深圳市前海安测信息技术有限公司 | Non-invasive detection system and method for diabetes and complications thereof |
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| CN2551996Y (en) * | 2002-07-16 | 2003-05-21 | 吉林大学化学学院 | Disposable colorimetric fast measuring bag |
| CN1570632A (en) * | 2003-07-17 | 2005-01-26 | 赵青华 | Biochemical detection liquid for glucose in urine |
| CN101614720A (en) * | 2009-07-29 | 2009-12-30 | 傅科杰 | Rapid detection method of free formaldehyde in textile and garment products |
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- 2012-07-25 CN CN2012102600063A patent/CN102749327A/en active Pending
Patent Citations (3)
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|---|---|---|---|---|
| CN2551996Y (en) * | 2002-07-16 | 2003-05-21 | 吉林大学化学学院 | Disposable colorimetric fast measuring bag |
| CN1570632A (en) * | 2003-07-17 | 2005-01-26 | 赵青华 | Biochemical detection liquid for glucose in urine |
| CN101614720A (en) * | 2009-07-29 | 2009-12-30 | 傅科杰 | Rapid detection method of free formaldehyde in textile and garment products |
Non-Patent Citations (3)
| Title |
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| 刘坤等: "AHMT分光光度法测定软骨鱼类硫酸软骨素", 《中国海洋药物》, vol. 24, no. 04, 31 August 2005 (2005-08-31) * |
| 王仲孚等: "用于寡糖链分析的HPLC柱前衍生化方法研究进展", 《有机化学》, vol. 26, no. 05, 31 May 2006 (2006-05-31), pages 592 - 598 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016074282A1 (en) * | 2014-11-11 | 2016-05-19 | 深圳市前海安测信息技术有限公司 | Non-invasive detection system and method for diabetes and complications thereof |
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Application publication date: 20121024 |