CN1565614A - Externally applied medicinal composition for resisting inflammation and repressing and killing bacteria and preparing method - Google Patents
Externally applied medicinal composition for resisting inflammation and repressing and killing bacteria and preparing method Download PDFInfo
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Abstract
The invention discloses an externally applied medicinal composition for resisting inflammation and repressing and killing bacteria and preparing method, wherein the composition is prepared from waras, cnidium fruit, Senecio scandens, amboyna extract, kuh-seng extract, acetic acid and borneol.
Description
Invention field
The present invention relates to a kind of pharmaceutical composition, particularly be used for antiinflammatory, antibacterial, germ-resistant topical composition, relate to the preparation method of said composition simultaneously.
Background technology
By cause a disease microbial gynecology colpitis, vulva pruritus such as mycete, antibacterial, and often bring misery to the patient by bacterial dermatosis, and easily recurrence.Side effect such as the antibiotics of Western medicine are bigger, existing Chinese medicine determined curative effect, easy to use less.The invention provides a kind of determined curative effect, side effect is little, Chinese medicine and western medicine topical composition easy to use.
Summary of the invention
One object of the present invention is to disclose a kind of topical composition that is used for the antiinflammatory bacteriostasis and sterilization; Another object of the present invention is the method for a kind of topical composition that is used for the antiinflammatory bacteriostasis and sterilization newly of open preparation.
Pharmaceutical composition of the present invention can be made up of following raw material medicaments respectively, and each crude drug component and proportioning are as follows:
A: Radix Flemingiae Philippinensis 10-30 weight portion Herba Senecionis Scandentis 5-15 weight portion.
Optimize proportioning: Radix Flemingiae Philippinensis 20 weight portion Herba Senecionis Scandentiss 10 weight portions.
Pharmaceutical composition of the present invention can increase crude drug on the basis of A, constitute the B scheme, and each crude drug component and proportioning are as follows:
B: Radix Flemingiae Philippinensis 10-30 weight portion Herba Senecionis Scandentis 5-15 weight portion Cortex Phellodendri extract 0.25-0.75 weight portion Radix Sophorae Flavescentis extract 1-3 weight portion Fructus Cnidii 5-15 weight portion Borneolum Syntheticum 0.4-1.2 weight portion
Optimize proportioning: Radix Flemingiae Philippinensis 20 weight portion Herba Senecionis Scandentiss 15 parts by volume Cortex Phellodendri extracts 0.5 weight portion Radix Sophorae Flavescentis extract 2 weight portion Fructus Cnidiis 10 weight portion Borneolum Syntheticums 0.8 weight portion
Pharmaceutical composition of the present invention can increase crude drug on the basis of B, constitute the C scheme, and each crude drug component and proportioning are as follows:
C: Radix Flemingiae Philippinensis 10-30 weight portion Herba Senecionis Scandentis 5-15 weight portion Cortex Phellodendri extract 0.25-0.75 weight portion Radix Sophorae Flavescentis extract 1-3 weight portion Fructus Cnidii 5-15 weight portion Borneolum Syntheticum 0.4-1.2 weight portion chlorhexidine acetate 1-3 weight portion
Optimize proportioning: Radix Flemingiae Philippinensis 20 weight portion Herba Senecionis Scandentiss 10 weight portion Cortex Phellodendri extracts 0.5 weight portion Radix Sophorae Flavescentis extract 2 weight portion Fructus Cnidiis 10 weight portion Borneolum Syntheticums 0.8 weight portion chlorhexidine acetate 2 weight portions
Described chlorhexidine acetate can be selected from product that sell in market.
C scheme preparation of compositions of the present invention becomes the washing liquid method to be:
More than seven the flavor, get Radix Flemingiae Philippinensis, Fructus Cnidii, Herba Senecionis Scandentis decocts with water 1-3 time, each 1-3 hour, collecting decoction filtered, filtrate is concentrated into 50-65 ℃, relative density is the clear paste of 1.15-1.30, puts coldly, adds ethanol and makes the alcohol amount of containing be 50-85%, stir evenly, left standstill 10-14 hour, and filtered filtrate recycling ethanol, the clear paste that to be concentrated into 50-65 ℃ of relative density be 1.05-1.20, add hot water and make dissolving in right amount, add Cortex Phellodendri extract again and make dissolving fully, add water to 800 parts by volume and stir evenly, add above-mentioned Radix Sophorae Flavescentis extract and chlorhexidine acetate (making dissolving fully with a small amount of dehydrated alcohol tepor in advance) successively, stir, centrifugal, filter, collect filtrate; Other gets Borneolum Syntheticum and adds a small amount of dehydrated alcohol and make dissolving, adds polyoxyethylene sorbitan monoleate, and mixing adds above-mentioned solution, add water to about 900-970 parts by volume, add sodium benzoate 1-4 weight portion again, make dissolving, transfer pH to 4.5-6.5 with 1M acetic acid, add water to 1000 parts by volume, shake up, promptly get washing liquid.Also can add other adjuvants, as essence, freshener.
Parts by volume is that milliliter is corresponding with gram with weight portion.
The present composition can also add excipient and make medicine, comes external as suppository, tablet, granule, effervescent tablet, medicine carrying cotton sliver.
Present composition preparation has good effect at the external antiinflammatory bacteriostasis and sterilization that is used for, and especially escherichia coli, staphylococcus aureus and Bai Nian bacterium is all had more antibacterial, bactericidal action, and is non-stimulated to vaginal mucosa.Be used for women mycete, infusorian, candidiasis, colpitis mycotica, cervicitis and pruritus vulvae, leucorrhea with red and white discharge, dysmenorrhea etc. and some dermatosiss of causing of bacterioid thus.
Following experimental example is used to further specify the present invention.It is washing liquid of the present invention that the present invention tests with preparation, and each scheme preparation of the present invention all can be realized following experimental example effect.
Experimental example 1Bacteriostasis
One, equipment:
1, test specimen: washing liquid of the present invention.
2, test strain: escherichia coli 8099, staphylococcus aureus ATCC 6538 (being called for short staphylococcus aureus down), Candida albicans ATCC 10231 (bacterium read in vain in following abbreviation).Provide by Military Medical Science Institute.Above strain algebraically was the 6th～10 generation, and prepared bacteria suspension with PBS solution.
3, test equipment: measuring pipette (0.1ml, 1.0ml, 5.0ml, 10.0ml), middle test tube, plate, water isolation type electro-heating standing-temperature cultivator (PYX-DHS-40 * 50) etc.
Two, method:
1, detects foundation: GB15979-2002 " disposable use hygienic article sanitary standard " appendix C product bactericidal property, bacteriostasis property and stability test method.Detect and repeat 3 times.
2, testing conditions: ambient temperature: 21～22 ℃, relative humidity: 68～76%.
Three, result:
Table 1 washing liquid bacteriostatic test of the present invention result
The average bacteriostasis rate and the scope of effect different time (min)
Sample
Strain (%) average control bacterium number (cfu/ml)
Character
2 5 10 20
Large intestine bar 100 100 100 100
Stock solution 2.18 * 10
4
Bacterium (100) (100) (100) (100)
Gold Fructus Vitis viniferae 100 100 100 100
Stock solution 2.19 * 10
4
Bacterium (100) (100) (100) (100)
100 100 100 100
Read bacterium stock solution 1.97 * 10 in vain
4
(100) (100) (100) (100)
Negative control: diluent, the equal asepsis growth of culture medium.
Four, conclusion: 2 minutes bacteriostasis rates to escherichia coli, staphylococcus aureus and Bai Nian bacterium of washing liquid stock solution of the present invention effect are 100%; Judge that then this product all has strong bacteriostasis to escherichia coli, staphylococcus aureus and Bai Nian bacterium.
Experimental example 2To staphylococcus aureus with colibacillaryly kill test
One, equipment
1, test strain: staphylococcus aureus ATCC6538 (being called for short staphylococcus aureus down); Escherichia coli 8099 are provided by Military Medical Science Institute.Above strain algebraically was the 6th～10 generation, and with containing 1% peptone PBS preparation bacteria suspension.
2, disinfectant: washing liquid of the present invention, make diluent with sterile distilled water.
3, nertralizer: contain 0.3% lecithin+2% Tween 80 0.03moL/LPBS solution.
4, carrier: white plain (1.0 * 1.0cm).
5, useless worker's suction pipe (0.1ml, 1.0ml, 5.0ml, 10.0ml) etc.
One, method
1, the preparation of bacterium sheet: according to Ministry of Public Health " disinfection technology standard " (1991.11) third edition first fascicle " experimental technique standard " 2.1, bacteria containing amount is 5 * 10
5～5 * 10
6The cfu sheet.
2, nertralizer qualification test: according in Ministry of Public Health " disinfection technology standard " (1999.11) third edition first fascicle " experimental technique standard " 2.4.Test organisms is a staphylococcus aureus.Test is grouped into: (1) disinfectant+bacterium sheet; (2) (disinfectant+bacterium sheet)+nertralizer; (3) nertralizer+bacterium sheet; (4) (disinfectant+nertralizer)+bacterium sheet; (5) PBS+ bacterium sheet; (6) test is used with batch PBS; (7) test is used with batch nertralizer; (8) test is used with batch culture medium.Disinfectant (25.0% diluent), 3min tested and repeated 3 times action time.19～21 ℃ of test temperatures
3, carrier soaks quantitative disinfecting test: experimentize according to 2.6.4.2 in Ministry of Public Health " disinfection technology standard " (1999.11) third edition first fascicle " experimental technique standard ".Disinfectant 25.0% diluent, 16.7% diluent, 12.5% diluent are 3,5,10,15min action time, and test repeats 3 times.19～21 ℃ of test temperatures.
One, result
1, nertralizer qualification test
The average growth of (1) group bacterium number is the 0cfu/ sheet, and the average growth of (2) group bacterium number is 1.50 * 10
2The cfu/ sheet, (3), the average growth of (4), (5) group bacterium number is close, between three groups error rate be 5.22%, the (6), (7), (8) group asepsis growth (table 2).
Table 2 nertralizer qualification test result
Each test growth bacterium number (cfu/ sheet) bacterium number of on average growing
Group 123 (cfu/ sheet)
1 0 0 0 0
2 2.30×10
2 1.20×10
2 1.00×10
2 1.50×10
2
3 9.10×10
5 7.50×10
5 8.40×10
5 8.33×10
5
4 8.50×10
5 6.40×10
5 7.80×10
5 7.57×10
5
5 8.30×10
5 6.00×10
5 7.50×10
5 7.27×10
5
6 0 0 0 0
7 0 0 0 0
8 0 0 0 0
2, with staphylococcus aureus and colibacillary killing effect
Show through 3 repeated trials: when 19～21 ℃ of test temperatures, this disinfectant 25.0% diluent, effect 3min and 16.7% diluent, effect 15min is respectively 99.97% and 99.95% to the killing rate of staphylococcus aureus.This disinfectant 25.0% diluent, effect 3min and 16.7% diluent, effect 15min is respectively 99.95% and 99.92% (table 3) to colibacillary killing rate.
Table 3 washing liquid of the present invention is to staphylococcus aureus and colibacillary killing effect
The average kill ratio and scope (%) average control of effect different time (min)
Bacterium
Disinfectant sample bacterium number (cfu/
Plant 35 10 15
Sheet
99.97 99.99 100 100
25.0% diluent
Gold (99.97-99.98) (99.99) (100) (100)
The Portugal
97.77 99.07 99.79 99.95
16.7% diluent
7.90×10
5
Ball
(96.96-98.28) (98.70-99.21) (99.75-99.82) (99.94-99.96)
Bacterium
80.75 88.77 97.39 99.76
12.5% diluent
(77.65-83.82) (85.78-91.91) (96.64-97.93) (99.66-99.80)
99.87 99.95 99.99 100
25.0% diluent
(99.86-99.87) (99.94-99.97) (99.99) (100) greatly
Intestinal
78.54 90.00 99.16 99.92
16.7% diluent
7.27×10
5
Bar
(73.80-81.60) (86.82-91.35) (98.87-99.40) (99.90-99.93)
Bacterium
60.66 70.42 96.09 99.70
12.5% diluent
(55.71-64.81) (67.77-72.46) (95.38-96.55) (99.62-99.76)
Negative control: diluent, the equal asepsis growth of culture medium.
One, conclusion
1, contains 0.3% lecithin+2% Tween 80 0.03moL/LPBS solution.This disinfectant that can effectively neutralize is to the effect of staphylococcus aureus, and this nertralizer and neutralized reaction product thereof have no adverse effects to test organisms and culture medium.
2, under experimental condition, this disinfectant 25.0% diluent, effect 3min and 16.7% diluent, effect 15min is respectively 99.97% and 99.95% to the killing rate of staphylococcus aureus.This disinfectant 25.0% diluent, effect 5min and 16.7% diluent, effect 15min is respectively 99.95% and 99.92% to colibacillary killing rate.
Experimental example 3Kill test to white is oidiomycetic
One, equipment
1, test strain: Candida albicans ATCC10231 (bacterium read in vain in following abbreviation) is provided by Military Medical Science Institute.Planting algebraically was the 6th～10 generation, and with containing 1% peptone PBS preparation bacteria suspension.
2, disinfectant: washing liquid of the present invention, make diluent with sterile distilled water.
3, nertralizer: contain 0.3% lecithin+2% Tween 80 0.03moL/LPBS solution.
4, carrier: white plain (1.0 * 1.0cm).
5, measuring pipette (0.1ml, 1.0ml, 5.0ml, 10.0ml) etc.
Two, method
1, the preparation of bacterium sheet: according to Ministry of Public Health " disinfection technology standard " (1991.11) third edition first fascicle " experimental technique standard " 2.1, bacterium sheet bacteria containing amount is 5 * 10
5～5 * 10
6The cfu sheet.
2, nertralizer qualification test: according in Ministry of Public Health " disinfection technology standard " (1999.11) third edition first fascicle " experimental technique standard " 2.4.Test organisms is a Candida albicans.Test is grouped into: (1) disinfectant+bacterium sheet; (2) (disinfectant+bacterium sheet)+nertralizer; (3) nertralizer+bacterium sheet; (4) (disinfectant+nertralizer)+bacterium sheet; (5) PBS+ bacterium sheet; (6) test is used with batch PBS; (7) test is used with batch nertralizer; (8) test is used with batch culture medium.This disinfectant stock solution, 5min tested and repeated 3 times action time.19～21 ℃ of test temperatures.
3, carrier soaks quantitative disinfecting test: " disinfection technology standard is according to 2.6.4.2 in Ministry of Public Health " disinfection technology standard " (1999.11) third edition first fascicle " experimental technique standard ", and 2.7 experimentize according to Ministry of Public Health.This disinfectant stock solution, 50.0% diluent, 25.0% diluent are 5,10,15,20min action time, and test repeats 3 times.19～21 ℃ of test temperatures.
Three, result
1, nertralizer qualification test
The average growth of (1) group bacterium number is the 0cfu/ sheet, and the average growth of (2) group bacterium number is 3.77 * 10
2The cfu/ sheet, (3), the average growth of (4), (5) group bacterium number is close, error rate is 5.13% between three groups.(6), (7), (8) group asepsis growth (table 4).
Table 4 nertralizer qualification test result
Each test growth bacterium number (cfu/ sheet) bacterium number of on average growing
Group 123 (cfu/ sheet)
1 0 0 0 0
2 2.90×10
2 4.10×10
2 4.30×10
2 3.77×10
2
3 7.40×10
5 8.40×10
5 9.40×10
5 8.40×10
5
4 7.10×10
5 7.60×10
5 8.30×10
5 7.67×10
5
5 6.9×10
5 7.1×10
5 8.00×10
5 7.33×10
5
6 0 0 0 0
7 0 0 0 0
8 0 0 0 0
2, dialogue is read the killing effect of bacterium
Show through 3 repeated trials: when 19～21 ℃ of test temperatures, this disinfectant stock solution, effect 5min and 50.0% diluent, effect 15min, the killing rate that dialogue is read bacterium is respectively 99.93% and 99.99% (table 5).
Table 5 washing liquid dialogue of the present invention is read the killing effect of bacterium
The average kill ratio and scope (%) average control of effect different time (min)
Disinfectant
Strain bacterium number (cfu/
Sample 5 10 15 20
Sheet)
99.93 99.99 100 100
Stock solution
Gold (99.91-99.95) (99.99) (100) (100)
Portugal 50% rare 98.93 99.87 99.99 100
6.70×10
5
Ball is released liquid (98.62-99.20) (99.84-99.89) 99.99 100
Bacterium 25% rare 73.28 87.22 95.88 98.77
(85.76-89.48) (95.38-96.46) (98.45-99.02) to release liquid (68.64-77.92)
Negative control: diluent, the equal asepsis growth of culture medium.
Four, conclusion
1, contains 0.3% lecithin+2% Tween 80 0.03moL/LPBS solution.This disinfectant dialogue that can effectively neutralize is read the effect of bacterium, and this nertralizer and neutralized reaction product thereof have no adverse effects to test organisms and culture medium.
2, under experimental condition, this disinfectant stock solution, effect 5min and 50.0% diluent, effect 15min, the killing rate that dialogue is read bacterium is respectively 99.93% and 99.99%.
Experimental example 4The acute oral toxicity experiment
One, material and animal:
1. tried thing: washing liquid of the present invention, liquid, raw sample supplies examination with distilled water preparation back.
2. animal: 20 of NIH mices (regular grade), male and female half and half, body weight 18～22g provides (quality certification 2001A044 of Guangdong Province number) by Guangdong Medical Lab Animal Center.
3. test condition: regular grade zoopery environmental facility (Guangdong Province Science and Technology Commission: Guangdong probatio inspectionem pecuoarem word 2002C001 number).
Two, method:
1. test basis: 3.4 (defending the method prison sends out [1999] No. 448) of " disinfection technology standard " (third edition) first fascicle experimental technique standard.
2. method: animal water supply fasting 12 hours, distilled water is a solvent, being tried thing, to establish dosage be 5500mg/kg, will be tried thing and once irritate to animal with irritating the stomach syringe needle by the 0.2ml/20g body weight.Observe and write down poisoning manifestations, death toll and the death time of animal after the administration immediately, observe secondary every day, two weeks of observation period.Carry out toxicity grading according to acute toxicity grading criteria.
Three, experimental result:
In observation period, tried the NIH mice and manifest symptom do not occurred, do not had dead the generation.
Table 6 is tried thing to NIHd, Mus acute oral toxicity
Sex dosage (mg/kg) number of animals (only) death toll (only) mortality rate (%)
Female 5,500 10 00
Male 5,500 10 00
Four, conclusion: washing liquid of the present invention is acute through LD50＞5000mg/kg to the NIH mice, the nontoxic level in true border.
Experimental example 5Vaginal mucomembranous irritant test
One, material and animal:
1. tried thing: washing liquid of the present invention, raw sample are directly for examination.
2. animal: 18 of female sd inbred rats (regular grade) about body weight 250g, provide (quality certification 2001A046 of Guangdong Province number) by Guangdong Medical Lab Animal Center.
3. test condition: regular grade zoopery environmental facility (Guangdong Province Science and Technology Commission: Guangdong probatio inspectionem pecuoarem word 2002C001 number):
Two, method:
1. test basis: 3.8 (defending the method prison sends out [1999] No. 448) of " disinfection technology standard " (third edition) first fascicle experimental technique standard.
2. method: set up to be subjected to examination group, excipient and blank group every group of 6 adult healthy female sd inbred rats.Soaked to be tried thing with the suitable sterilization absorbent cotton of size by the examination group places and is tried in the SD rat vagina, vehicle group is soaked sterile saline with onesize cotton sliver and is placed and tried in the SD rat vagina, all contact 24h with the animal vaginal mucosa, the blank group does not then give any processing.Put to death animal behind the 24h, take out whole vagina, observe hyperemia, the edema reacting phenomenon of local vagina tissue, carry out the irritant reaction scoring.
Three, experimental result:
Be subjected to the local vagina tissue of examination group and matched group SD rat all not find abnormal responses such as hyperemia, edema, also do not find other symptom.Vaginal mucosa irritation index=0.0 (seeing Table 7).
Table 7 is tried thing to SD rat vagina mucous membrane irritation reaction scoring
The scoring of number of animals irritant reaction
Group
(only) congestion and edema total points
Contamination organizes 6000
Vehicle group 6000
Blank group 6000
Annotate: in irritant reaction scoring hurdle, congested reaction scoring and edema reaction are respectively the meansigma methods of each treated animal; Total points is congested reaction scoring and edema reaction scoring sum.
Four, conclusion:
Washing liquid of the present invention belongs to nonirritant to the vaginal mucosa irritation of female sd inbred rats.
Experimental example 4Stability test
One, equipment:
1, test specimen: washing liquid of the present invention.
2, test strain: Candida albicans ATCC10231 (bacterium read in vain in following abbreviation).Provide by Military Medical Science Institute.Above strain algebraically was the 6th～10 generation, and prepared bacteria suspension with PBS solution.
3, test equipment: measuring pipette (0.1ml, 1.0ml, 5.0ml, 10.0ml), middle test tube, plate, water isolation type electro-heating standing-temperature cultivator (PYX-DHS-40 * 50) etc.
Two, method:
1, detects foundation: GB15979-2002 " disposable use hygienic article sanitary standard " appendix C product bactericidal property, bacteriostasis property and stability test method.Detect and repeat 3 times.
2, testing conditions: ambient temperature: 21～22 ℃, relative humidity: 68～76%.
Three, result:
Table 8 washing liquid of the present invention is to the stability test of Candida albicans fungistatic effect
The average kill ratio and the scope (%) of effect different time (min) are on average right
Deposit
Place temperature according to the bacterium number
Time
Degree (℃) 25 10 20 (cfu/
(my god) sheet)
100 100 100 100
0
(100) (100) (100) (100) 1.72×1
54
73.60 81.89 90.92 94.90 04
14
(71.96-76.22)?(79.68-84.96)?(86.88-93.29)?(92.22-96.32)
Negative control: diluent, the equal asepsis growth of culture medium.
Four, conclusion: washing liquid of the present invention is after 54 ℃ of incubators are placed 14 days (test is valid for one year), and its stock solution effect 2 clocks are 73.60% to the oidiomycetic bacteriostasis rate of white.
The following example all can be realized the effect of above-mentioned experimental example.
Embodiment 1Washing liquid
Radix Flemingiae Philippinensis 20g Herba Senecionis Scandentis 10g Fructus Cnidii 10g Cortex Phellodendri extract 0.5g Radix Sophorae Flavescentis extract 2g Borneolum Syntheticum 0.8g chlorhexidine acetate 2g.
More than seven the flavor, get Radix Flemingiae Philippinensis, Fructus Cnidii, Herba Senecionis Scandentis decocts with water secondary, each 1.5 hours, collecting decoction filtered, the clear paste that it is 1.20-1.25 that filtrate is concentrated into 60 ℃ of relative densities, put coldly, add ethanol and make that to contain alcohol amount be 65%, stir evenly, left standstill 12 hours, filter filtrate recycling ethanol, the clear paste that to be concentrated into 60 ℃ of relative densities be 1.10-1.20, add hot water and make dissolving in right amount, add Cortex Phellodendri extract again and make dissolving fully, add water to 800ml and stir evenly, filter, add above-mentioned Radix Sophorae Flavescentis extract and chlorhexidine acetate (making dissolving fully with a small amount of dehydrated alcohol tepor in advance) in the filtrate successively, stir, centrifugal, filter, collect filtrate; Other gets Borneolum Syntheticum and adds a small amount of dehydrated alcohol and make dissolving, adds polyoxyethylene sorbitan monoleate, and mixing adds above-mentioned solution, adds water to about 950ml, add sodium benzoate 2g again, make dissolving, transfer pH to 4.5-6.5, add water to 1000ml with 1M acetic acid, shake up, fill, every 20ml, promptly.Gynecological inflammation, earlier with the water purification cleaning part, dry, wash in right amount or demibain with this product 1-2 bottle boiled water of heating then.Every day 1-2 time; Or directly be coated with the affected part with this product.Other relevant diseases can directly be applied to the affected part.
Embodiment 2Suppository
Radix Flemingiae Philippinensis 25g Herba Senecionis Scandentis 13g Fructus Cnidii 8g Cortex Phellodendri extract 0.7g Radix Sophorae Flavescentis extract 2g Borneolum Syntheticum 1g.
More than seven the flavor, get Radix Flemingiae Philippinensis, Fructus Cnidii, Herba Senecionis Scandentis decocts with water three times, each 2 hours, collecting decoction filtered, the clear paste that it is 1.20-1.28 that filtrate is concentrated into 65 ℃ of relative densities is put coldly, adds ethanol and makes that to contain alcohol amount be 70%, stir evenly, left standstill 10 hours, filter, filtrate recycling ethanol, the clear paste that to be concentrated into 65 ℃ of relative densities be 1.05-1.20 is got semi-synthetic fatty acid glyceride, puts the heating in water bath fusing, treat that temperature reduces to 50 ℃, other gets the Borneolum Syntheticum porphyrize, adds in the good substrate of fusion, with adding with stirring, add Cortex Phellodendri extract then successively, Radix Sophorae Flavescentis extract and sodium benzoate, fully grinding is evenly poured in the mould, makes 50 altogether, solidify, wipe off, take out, packing promptly.
Embodiment 3Suppository
Radix Flemingiae Philippinensis 20g Herba Senecionis Scandentis 15g Cortex Phellodendri extract 0.5g Radix Sophorae Flavescentis extract 2g Borneolum Syntheticum 0.8g Fructus Cnidii 10g.
More than seven the flavor, get Radix Flemingiae Philippinensis, Fructus Cnidii, Herba Senecionis Scandentis decocts with water three times, each 2 hours, collecting decoction filtered, the clear paste that it is 1.20-1.28 that filtrate is concentrated into 65 ℃ of relative densities is put coldly, adds ethanol and makes that to contain alcohol amount be 70%, stir evenly, left standstill 10 hours, filter, filtrate recycling ethanol, the clear paste that to be concentrated into 65 ℃ of relative densities be 1.05-1.20 is got semi-synthetic fatty acid glyceride, puts the heating in water bath fusing, treat that temperature reduces to 50 ℃, other gets the Borneolum Syntheticum porphyrize, adds in the good substrate of fusion, with adding with stirring, add Cortex Phellodendri extract then successively, Radix Sophorae Flavescentis extract and sodium benzoate, fully grinding is evenly poured in the mould, makes 50 altogether, solidify, wipe off, take out, packing promptly.
Embodiment 4Washing liquid
Radix Flemingiae Philippinensis 250g Herba Senecionis Scandentis 140g
More than two the flavor, decoct with water secondary, each 1.5 hours, collecting decoction, filter, the clear paste that it is 1.20-1.25 that filtrate is concentrated into 60 ℃ of relative densities is put coldly, adds ethanol and makes that to contain the alcohol amount be 65%, stir evenly, left standstill 12 hours, filter filtrate recycling ethanol, the clear paste that to be concentrated into 60 ℃ of relative densities be 1.10-1.20 adds water to about 950ml, adds sodium benzoate 2g again, make dissolving, transfer pH to 4.5-6.5, add water to 10000ml with 1M acetic acid, shake up, fill, promptly.Gynecological inflammation cleans affected part, dries with water purification earlier, and heating with this product 20-40ml then, boiled water is an amount of to be washed or demibain.Every day 1-2 time; Or directly be coated with the affected part with this product.Other relevant diseases can directly be applied to the affected part.
Embodiment 5Washing liquid
Radix Flemingiae Philippinensis 200g Herba Senecionis Scandentis 100g.
More than two the flavor, decoct with water secondary, each 1.5 hours, collecting decoction, filter, the clear paste that it is 1.20-1.25 that filtrate is concentrated into 60 ℃ of relative densities is put coldly, adds ethanol and makes that to contain the alcohol amount be 65%, stir evenly, left standstill 12 hours, filter filtrate recycling ethanol, the clear paste that to be concentrated into 60 ℃ of relative densities be 1.10-1.20 adds water to about 950ml, adds sodium benzoate 2g again, make dissolving, transfer pH to 4.5-6.5, add water to 10000ml with 1M acetic acid, shake up, fill, promptly.Gynecological inflammation cleans affected part, dries with water purification earlier, and heating with this product 20-40ml then, boiled water is an amount of to be washed or demibain.Every day 1-2 time; Or directly be coated with the affected part with this product.Other relevant diseases can directly be applied to the affected part.
Claims (11)
1. externally-applied medicinal composition with antiinflammatory bacteriostasis and sterilization is characterized in that this pharmaceutical composition made by following raw material medicaments:
Radix Flemingiae Philippinensis 10-30 weight portion Herba Senecionis Scandentis 5-15 weight portion.
2. pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:
Radix Flemingiae Philippinensis 20 weight portion Herba Senecionis Scandentiss 10 weight portions.
3. pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:
Radix Flemingiae Philippinensis 10-30 weight portion Herba Senecionis Scandentis 5-15 weight portion Cortex Phellodendri extract 0.25-0.75 weight portion Radix Sophorae Flavescentis extract 1-3 weight portion Borneolum Syntheticum 0.4-1.2 weight portion Fructus Cnidii 5-15 weight portion.
4. pharmaceutical composition as claimed in claim 3 is characterized in that this pharmaceutical composition made by following raw material medicaments:
Radix Flemingiae Philippinensis 20 weight portion Herba Senecionis Scandentiss 15 weight portion Cortex Phellodendri extracts 0.5 weight portion Radix Sophorae Flavescentis extract 2 weight portion Borneolum Syntheticums 0.8 weight portion Fructus Cnidii 10 weight portions.
5. pharmaceutical composition as claimed in claim 1 is characterized in that this pharmaceutical composition made by following raw material medicaments:
Radix Flemingiae Philippinensis 10-30 weight portion Herba Senecionis Scandentis 5-15 weight portion Cortex Phellodendri extract 0.25-0.75 weight portion Radix Sophorae Flavescentis extract 1-3 weight portion Fructus Cnidii 5-15 weight portion Borneolum Syntheticum 0.4-1.2 weight portion chlorhexidine acetate 1-3 weight portion.
6. pharmaceutical composition as claimed in claim 5 is characterized in that this pharmaceutical composition made by following raw material medicaments:
Radix Flemingiae Philippinensis 20 weight portion Herba Senecionis Scandentiss 10 weight portion Cortex Phellodendri extracts 0.5 weight portion Radix Sophorae Flavescentis extract 2 weight portion Fructus Cnidiis 10 weight portion Borneolum Syntheticums 0.8 weight portion chlorhexidine acetate 2 weight portions.
7. as the described pharmaceutical composition of claim 1-6, it is characterized in that this pharmaceutical composition also can add excipient and make washing liquid, capsule, suppository, tablet, granule, effervescent tablet, medicine carrying cotton sliver.
8, as claim 5 or 6 described pharmaceutical compositions, it is characterized in that this preparation of pharmaceutical compositions becomes the method for lotion to be: get Radix Flemingiae Philippinensis, Fructus Cnidii, Herba Senecionis Scandentis, decoct with water 1-3 time, each 1-3 hour, collecting decoction filtered, the clear paste that it is 1.15-1.30 that filtrate is concentrated into 50-65 ℃ of relative density, put coldly, add ethanol and make the alcohol amount of containing be 50-80%, stir evenly, left standstill 10-14 hour, filter filtrate recycling ethanol, the clear paste that to be concentrated into 50-65 ℃ of relative density be 1.05-1.20, add hot water and make dissolving in right amount, add Cortex Phellodendri extract again and make dissolving fully, add water to 800 parts by volume and stir evenly, filter, add above-mentioned Radix Sophorae Flavescentis extract and chlorhexidine acetate in the filtrate successively, stir, centrifugal, filter, collect filtrate; Other gets Borneolum Syntheticum and adds a small amount of dehydrated alcohol and make dissolving, adds polyoxyethylene sorbitan monoleate, and mixing adds above-mentioned solution, add water to about 900-970 parts by volume, add sodium benzoate 1-4 weight portion again, make dissolving, transfer pH to 4.5-6.5 with 1M acetic acid, add water to 1000 parts by volume, shake up, promptly.
9. preparation of drug combination method as claimed in claim 8 is characterized in that this method is: get Radix Flemingiae Philippinensis, Fructus Cnidii, Herba Senecionis Scandentis decocts with water secondary, each 1.5 hours, collecting decoction filtered, the clear paste that it is 1.20-1.25 that filtrate is concentrated into 60 ℃ of relative densities, put coldly, add ethanol and make that to contain alcohol amount be 65%, stir evenly, left standstill 12 hours, filter filtrate recycling ethanol, the clear paste that to be concentrated into 60 ℃ of relative densities be 1.10-1.20, add hot water and make dissolving in right amount, add Cortex Phellodendri extract again and make dissolving fully, add water to 800ml and stir evenly, filter, add above-mentioned Radix Sophorae Flavescentis extract and chlorhexidine acetate in the filtrate successively, stir, centrifugal, filter, collect filtrate; Other gets Borneolum Syntheticum and adds a small amount of dehydrated alcohol and make dissolving, adds polyoxyethylene sorbitan monoleate 7.5g again, and mixing adds above-mentioned solution, adds water to about 950ml, adds sodium benzoate 2g again, makes dissolving, transfers pH to 4.5-6.5 with 1M acetic acid, adds water to 1000ml, shakes up, promptly.
10, has application in the external used medicine of effects of antiinflammation and bacteriostasis as the described pharmaceutical composition of claim 1-6 in preparation.
11, application as claimed in claim 11 is characterized in that killing application in escherichia coli, staphylococcus aureus and the Candida albicans external used medicine in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB03148879XA CN1239169C (en) | 2003-06-16 | 2003-06-16 | Externally applied medicinal composition for resisting inflammation and repressing and killing bacteria and preparing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB03148879XA CN1239169C (en) | 2003-06-16 | 2003-06-16 | Externally applied medicinal composition for resisting inflammation and repressing and killing bacteria and preparing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1565614A true CN1565614A (en) | 2005-01-19 |
| CN1239169C CN1239169C (en) | 2006-02-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB03148879XA Expired - Lifetime CN1239169C (en) | 2003-06-16 | 2003-06-16 | Externally applied medicinal composition for resisting inflammation and repressing and killing bacteria and preparing method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101947269A (en) * | 2010-09-03 | 2011-01-19 | 刘安庆 | Medicine for treating viral herpes |
| CN103381253A (en) * | 2013-06-27 | 2013-11-06 | 黄文珍 | Traditional Chinese medicine composite for curing female pruritus vulvue and preparation method thereof |
| CN107149569A (en) * | 2017-03-27 | 2017-09-12 | 广西厚德大健康产业股份有限公司 | A kind of antipruritic skin gels composition |
| CN110433284A (en) * | 2019-08-07 | 2019-11-12 | 广东圆康再生医学科技开发有限公司 | A kind of composition that treating cervicitis, gelling agent and preparation method thereof |
| CN112136838A (en) * | 2020-08-27 | 2020-12-29 | 东莞市泰赛特汽车用品科技有限公司 | Mild and nontoxic sterilization disinfectant, preparation method and application thereof |
-
2003
- 2003-06-16 CN CNB03148879XA patent/CN1239169C/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101947269A (en) * | 2010-09-03 | 2011-01-19 | 刘安庆 | Medicine for treating viral herpes |
| CN103381253A (en) * | 2013-06-27 | 2013-11-06 | 黄文珍 | Traditional Chinese medicine composite for curing female pruritus vulvue and preparation method thereof |
| CN103381253B (en) * | 2013-06-27 | 2015-03-25 | 黄文珍 | Traditional Chinese medicine composite for curing female pruritus vulvue and preparation method thereof |
| CN107149569A (en) * | 2017-03-27 | 2017-09-12 | 广西厚德大健康产业股份有限公司 | A kind of antipruritic skin gels composition |
| CN110433284A (en) * | 2019-08-07 | 2019-11-12 | 广东圆康再生医学科技开发有限公司 | A kind of composition that treating cervicitis, gelling agent and preparation method thereof |
| CN112136838A (en) * | 2020-08-27 | 2020-12-29 | 东莞市泰赛特汽车用品科技有限公司 | Mild and nontoxic sterilization disinfectant, preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1239169C (en) | 2006-02-01 |
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