CN1552500A - Assembled chromatography dry column - Google Patents
Assembled chromatography dry column Download PDFInfo
- Publication number
- CN1552500A CN1552500A CNA031335063A CN03133506A CN1552500A CN 1552500 A CN1552500 A CN 1552500A CN A031335063 A CNA031335063 A CN A031335063A CN 03133506 A CN03133506 A CN 03133506A CN 1552500 A CN1552500 A CN 1552500A
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- CN
- China
- Prior art keywords
- ring seal
- column
- band
- post
- end cap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Treatment Of Liquids With Adsorbents In General (AREA)
- Gasket Seals (AREA)
Abstract
A combined main chromatographic column is composed of the end cover of column, the flange of said end cover, and multiple column rings with tenon or mortise structure at both ends for combining then together via sealing ring, locating holes and locating axles. Its advantages are high flowing speed and easy discharge of adsorbent.
Description
Technical field:
The present invention relates to the dried post of combined type chromatogram, particularly relate to the dried post of the chromatogram of forming by end cap, flange, the band of column, O-ring seal, filter plate and fastening bolt.
Background technology:
Dry column chromatography is to utilize the difference for the treatment of branch sample chromatographic behavior on dry adsorption stuffing, reaches a kind of chromatographic technique that separates and prepare purpose.It is operated in the chromatographic column of normally dry adsorption stuffing being packed into, goes up sample after sample to be separated is made concentrate or is adsorbed in a small amount of carrier.During chromatography, eluent relies on capillary action to flow down from post top to carry out wash-out, when eluent during near the chromatographic column bottom, stops wash-out.Through colour developing, adsorption stuffing in the chromatography rear pillar is pressed the different cutting and separating of colour band on the post, extract or wash out target substance with appropriate solvent.
Dry column chromatography has separates the advantage of rendeing a service height, weak point consuming time, saving solvent, enjoys favor always.At present the dried post of chromatogram that uses has two types of plastic column and glass columns.The dried post of plastics (Love B, Goodman MM.1967, Chem Ind 2026) makes things convenient for after the chromatogram cutting and separates, but the dress post, go up sample and wash-out extremely difficult, the homogeneity of elution process center pillar bed is difficult to assurance.Glass column (Beveridge1988) obviously is not easy to separation and cutting behind the wash-out though be convenient to adorn post and last sample.In addition, when using tiny granularity adsorption stuffing to separate, the elution speed of above-mentioned two kinds of chromatographic columns is extremely slow, and condition is restive, and separating resulting is difficult to repetition.
It is a kind of that J.T.Baker recommends " high-resolution prepares the type assembly " (it is made of several piece glass or quartz component for preparative high resolutionsegment, PHS) device, and each assembly borrows interface to be coupled to each other into post, ascending development in the cover of sealing.The PHS device has made things convenient for the location of filler to cut apart, but is subjected to the restriction of structure and material, and power only is capillary tension, can not use the fine grain filler, and the homogeneity of loading density is difficult to guarantee simultaneously, and problem consuming time also fails to solve.Nineteen eighty-two, Leopold has developed the dried post of a kind of decompression chromatogram, and the latter has only partly solved problem consuming time.
In sum, though dry column chromatography has some advantages, because the inconvenience of existing application device also fails to obtain excellent popularization and application.
Technology contents:
The objective of the invention is to solve that the flow velocity that the dried post of present chromatogram exists is slow, the time long, adsorbent takes out problems such as inconvenience, provide simple, efficient, convenient and the dried post of chromatogram fast.
The invention provides the dried post of a kind of combined type chromatogram, it is characterized in that:
This chromatographic column mainly comprises the assembly of styletable lid, end cap flange, three kinds of rigidity of the band of column; The two ends of the described band of column are respectively equipped with the ring groove and the bulge loop that compresses O-ring seal for the O-ring seal placement at the tenon fourth of the twelve Earthly Branches each other, and the two ends of the band of column are provided with the pilot hole and the locating shaft at the tenon fourth of the twelve Earthly Branches each other simultaneously; One end of described end cap flange is provided with and an end of the band of column ring groove of placing for O-ring seal at the tenon fourth of the twelve Earthly Branches or the bulge loop that compresses O-ring seal each other, and pilot hole or locating shaft; One end of described styletable lid is provided with the sealing surface with end cap flange coupling, and periphery has the through hole with the corresponding ccontaining fastening bolt of end cap flange;
Several band of columns that have O-ring seal are chimeric as a whole mutually, and two ends are chimeric end cap flange, filter plate, O-ring seal, styletable lid respectively, again by the fastening post that is assembled into of fastening bolt.
In the dried post of combined type chromatogram of the present invention, the bulge loop that compresses O-ring seal on the band of column end face can be parallel with end face with the surface of contact of O-ring seal, also can be the inclined-plane; When the surface of contact that compresses the bulge loop of O-ring seal and O-ring seal on the band of column end face is the inclined-plane, can adopt the trapezoidal O-ring seal that mates fully with the inclined-plane, also can adopt: " O " RunddichtringO; When adopting trapezoidal O-ring seal, bevel angle is 27～30 °; When adopting " O " RunddichtringO, bevel angle is 35～45 °; The end cap flange has location and the hermetically-sealed construction with the band of column and styletable coupling.The ring groove of placing for O-ring seal on the band of column can with pilot hole at same end face, also can with locating shaft at same end face.
Adopt the such structure of the present invention, connect locating shaft and pilot hole relation assembly and disassembly easily according to styletable lid, end cap flange and the band of column; Screw fastening bolt, styletable lid, end cap flange and the band of column can be realized excellent sealing by O-ring seal, make post bear tens of kilograms of pressure, and chromatographic column can help using the fine grain filler at chromatography under the pressurized conditions, can effectively improve resolving power, shortens the chromatography time; In addition, after chromatography finished, the location cutting of adsorbent was very convenient.In a word, the invention solves that the flow velocity that the dried post of present chromatogram exists is slow, the time long, adsorbent takes out problems such as inconvenience, provide simple, efficient, convenient and the dried post of chromatogram fast, can be widely used in research and the mask work produced in.
Description of drawings:
Fig. 1 is the cut-open view of dried first embodiment of post of combined type chromatogram of the present invention;
Fig. 2 is the cut-open view of the band of column of dried first embodiment of post of combined type chromatogram of the present invention;
Fig. 3 is the cut-open view of the band of column of dried another embodiment of post of combined type chromatogram of the present invention;
Fig. 4 is the cut-open view of the trapezoidal O-ring seal of the dried post of combined type chromatogram of the present invention;
Fig. 5 is the cut-open view of the dried post of combined type chromatogram of the present invention " O " shape O-ring seal.
Embodiment:
The dried post of combined type chromatogram shown in Figure 1, be made up of styletable lid 1, end cap flange 2, the band of column 3, O-ring seal 4, filter plate 5 and fastening bolt 6: an end of the band of column has pilot hole 7, and the other end has locating shaft 8; One end of the band of column has the ring groove of placing for O-ring seal 9, and the other end of the band of column has the bulge loop 10 that compresses O-ring seal; The surface of contact of bulge loop and O-ring seal is plane 11, adopts " O " RunddichtringO; The end cap flange has location and the hermetically-sealed construction with the band of column and styletable coupling.
Connect locating shaft 8 and pilot hole 7 relations are assembled into post according to styletable lid 1, end cap flange 2 and the band of column 3; Screw fastening bolt 6, make styletable lid 1, end cap flange 2 and the band of column 3 realize excellent sealing by " O " O-ring seal 14; Select 5～63 μ m fillers, dry column-packing, last sample and seal chromatographic column after, chromatography pressurizes under＜10 bar pressures; Chromatography after finishing is taken post apart, location cutting adsorbent.
The dried post of combined type chromatogram of the present invention also can adopt other version, as the bulge loop that compresses O-ring seal on the band of column end face and the surface of contact of O-ring seal can be inclined-plane 12, when surface of contact is inclined-plane 12, can adopt the trapezoidal O-ring seal 13 that mates fully with the inclined-plane, also can adopt " O " RunddichtringO 14; When adopting trapezoidal O-ring seal 13, bevel angle is 27～30 °; When adopting " O " RunddichtringO 14, bevel angle is 35～45 °; Also can not have the bulge loop that compresses O-ring seal on the band of column end face, at this moment, the degree of depth of the ring groove of placing for O-ring seal on another adjacent with it band of column end face will be provided with more shallow, so that make O-ring seal can give prominence to band of column end face.The ring groove of placing for O-ring seal on the band of column 9 can with pilot hole 7 at same end face, also can with locating shaft 8 at same end face.
Claims (4)
1, the dried post of a kind of combined type chromatogram is characterized in that:
This chromatographic column mainly comprises the assembly of styletable lid (1), end cap flange (2), (3) three kinds of rigidity of the band of column; Ring groove (9) that is provided with the confession O-ring seal placement at the tenon fourth of the twelve Earthly Branches each other in addition and the bulge loop (10) that compresses O-ring seal are divided in the two ends of the described band of column (3), and the two ends of the band of column are provided with pilot hole at the tenon fourth of the twelve Earthly Branches (7) and locating shaft (8) each other simultaneously; One end of described end cap flange (2) is provided with and an end of the band of column (3) ring groove of placing for O-ring seal (9) at the tenon fourth of the twelve Earthly Branches or the bulge loop (10) that compresses O-ring seal each other, and pilot hole (7) or locating shaft (8); One end of described styletable lid (1) is provided with the sealing surface with end cap flange (2) coupling, and periphery has the through hole of corresponding ccontaining fastening bolt with end cap flange (2) (6);
Several band of columns (3) that have O-ring seal (4) are chimeric as a whole mutually, and two ends are chimeric end cap flange (2), filter plate (5), O-ring seal (4), styletable lid (1) respectively, again by the fastening post that is assembled into of fastening bolt (6).
2, according to the dried post of the described combined type chromatogram of claim 1, it is characterized in that: compress the bulge loop and the surface of contact of O-ring seal parallel with end face (11) of O-ring seal on the described band of column (3) end face, or inclined-plane (12).
3, according to the dried post of the described combined type chromatogram of claim 2, it is characterized in that: when the surface of contact of bulge loop that compresses O-ring seal on the described band of column (3) end face and O-ring seal is inclined-plane (12), adopt the trapezoidal O-ring seal (13) that mates fully with the inclined-plane, perhaps adopt " O " RunddichtringO (14).
4, according to claim 1,2, the dried post of 3 described combined type chromatograms, it is characterized in that: when adopting trapezoidal O-ring seal (13), bevel angle is 27～30 °; When adopting " O " RunddichtringO (14), bevel angle is 35～45 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133506 CN1277594C (en) | 2003-05-28 | 2003-05-28 | Assembled chromatography dry column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133506 CN1277594C (en) | 2003-05-28 | 2003-05-28 | Assembled chromatography dry column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1552500A true CN1552500A (en) | 2004-12-08 |
| CN1277594C CN1277594C (en) | 2006-10-04 |
Family
ID=34323056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03133506 Expired - Fee Related CN1277594C (en) | 2003-05-28 | 2003-05-28 | Assembled chromatography dry column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1277594C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101912697A (en) * | 2010-08-13 | 2010-12-15 | 利穗科技(苏州)有限公司 | Quick-release type large-volume preparative chromatographic column |
| CN103752040A (en) * | 2014-01-07 | 2014-04-30 | 南京诺云生物科技有限公司 | Modularized chromatographic column and purposes thereof |
-
2003
- 2003-05-28 CN CN 03133506 patent/CN1277594C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101912697A (en) * | 2010-08-13 | 2010-12-15 | 利穗科技(苏州)有限公司 | Quick-release type large-volume preparative chromatographic column |
| CN101912697B (en) * | 2010-08-13 | 2012-05-23 | 利穗科技(苏州)有限公司 | Quick-release type large-volume preparative chromatographic column |
| CN103752040A (en) * | 2014-01-07 | 2014-04-30 | 南京诺云生物科技有限公司 | Modularized chromatographic column and purposes thereof |
| CN103752040B (en) * | 2014-01-07 | 2016-06-22 | 南京诺云生物科技有限公司 | Modularity chromatographic column and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1277594C (en) | 2006-10-04 |
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| SE01 | Entry into force of request for substantive examination | ||
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Granted publication date: 20061004 |