CN1552465A - Fiber protein stopping bleeding paste - Google Patents
Fiber protein stopping bleeding paste Download PDFInfo
- Publication number
- CN1552465A CN1552465A CNA031267629A CN03126762A CN1552465A CN 1552465 A CN1552465 A CN 1552465A CN A031267629 A CNA031267629 A CN A031267629A CN 03126762 A CN03126762 A CN 03126762A CN 1552465 A CN1552465 A CN 1552465A
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- collagen sponge
- hemostasis
- tendon
- fibrin
- solution
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Abstract
A staltic fibrin picking used as the absorbable medical dressing is prepared from the leg's muscle tendon of pig, ox, or horse through preparing collegen sponge and coating fibrinogen and thrombase. An absorbabal staltic fibrin bandage is also disclosed, which is prepared from said staltic fibrin picking and conventional staltic bandage.
Description
One, technical field: the present invention relates to a kind of absorbable medical dressing that is used for internal organs and body surface wound hemostasis
Two, technical background:
The surgery of hospital (operation) hemostatic treatment, many at present employing gelfoam, but this material hydrophilic is poor, haemostatic effect is not good enough.What enter the domestic market in recent years separates Er Feisi (Gelfix, the collagen sponge that U.S. a company provides), and effect is remarkable in hemostasis and wound repair treatment, but valuable product, specification are single.Collagen sponge (comprise and separate Er Feisi) is that molecular weight is that about 300,000 gel monomer original molecule is arranged the fibre structure that forms, when contacting with wound surface, except that the absorption platelet promotes the coagulation process, also can activate and induce various somatomedin, promote fibroblast proliferation and from the generation and the arrangement of body collagen fiber, thereby promote the generation of granulation tissue.
Collagen sponge has following characteristics in the surgery hemostatic treatment:
(1) this material hydrophilic is good, and bleeding stopping period is short, adheres to the ability excellence of wound surface, and haemostatic effect is better than gelfoam.
(2) spread on surface wound, the wound surface drying, the difficult infection, wound healing is rapid; After the internal organs of the body hemostasis, local no abnormality seen irritant reaction, absorbed is good.
Tachocomb (TachoComb, U.S.'s product) has added Fibrinogen, thrombin, aprotinin on collagen sponge, strengthened hemostatic function.The clinical practice reflection is fine, but the very expensive (30cm of price
2Tachocomb need more than 2,000 yuan of RMB), be difficult in China and apply.This invention (the fibrin hemostasis is pasted) is exactly the succedaneum of this product, and price is far below Tachocomb.
The fibrin sealant tourniquet bandage
AUS Wal spy. Reed institute and American Red Cross develop a kind of tourniquet bandage that scribbles haemproteins, and the patient who bleeds in a large number because of wound is stopped blooding rapidly.This novel binder just contains a large amount of dried Fibrinogen and thrombin in 10 square centimeters.As long as contact with wound, the formation fibrin that will combine with blood, and knot is silly rapidly, occurs gluey sealant on wound, can make serious arterial hemorrhage person's hemostasis in 2 minutes.
This tourniquet bandage at ordinary times (as disaster) and wartime (extensive injures and deaths) can handle large quantities of wounded rapidly, reduce mortality rate significantly.
Above-mentioned several medical material still is in blank or starting stage in the application of China mainland, and market prospect is wide.
Three, summary of the invention:
1. collagen sponge production technology
The tendon of carefully isolating the tendon → pulverizing tendon of the leg of pork → after will pulverizing is soaked in 0.1% the acetic acid solution, wherein add behind an amount of pepsin fully mixing → under room temperature (or 2 ℃～8 ℃), be saved to solution and reach the suitable viscosity after-filtration slagging-off → collagen solution of 98-99% and the glycerol of 1-2% are mixed, freezingly obtain 100～150 ℃ of spongy materials → place fixedly 60-120 minute down, obtain medical collagen sponge.Technique scheme is compared simpler and more direct with other similar technology, its raw material source is abundanter, cheap, and the collagen of preparation is more natural, pure, safety (then easily propagating bovine spongiform encephalopathy or foot and mouth disease as the heel string with horse or cattle).
2. the fibrin hemostasis is pasted
Production technology: with riboflavin (7～26 μ g/cm
2), Fibrinogen (4.3～6.7mg/cm of from Sanguis sus domestica, extracting
2) and thrombin (1.5～2.5IU/cm
2) lyophilized powder adds in the mortar fully porphyrize according to quantity, mix homogeneously → adding absolute ethyl alcohol and stirring becomes uniform suspension → pour in the stainless steel disc, concussion makes particle uniform distribution → one side that collagen sponge is smooth be affixed on and try to get to the heart of a matter, above-mentioned three kinds of granules promptly are attached on the collagen sponge → the evacuation drying the fibrin hemostasis paste → tailor into different size → encapsulation afterwards cobalt source radiation sterilization, the virus of going out.
During with general Fibrin Glue, to mix fibrinogen solution and thrombin solution earlier, again mixed liquor be applied on wound, utilize biochemical hemostasis reaction, only can stop the hemorrhage of tiny vein and blood capillary; During with product of the present invention, Fibrinogen and thrombin have been attached on the solid gum olynthus, can apply immediately on wound, just can dissolve after Fibrinogen and thrombin and blood, lymph fluid or the contact with normal saline, the blood coagulation reaction promptly begins (biochemical hemostasis), collagen sponge becomes the surface (machinery hemostasis) that thin and fine and close dressing is attached on wound or organ tightly immediately simultaneously, have biochemical hemostasis and mechanical hemostatic function simultaneously, improve haemostatic effect greatly, can stop the hemorrhage of small artery and following blood vessel.This hemostasis is pasted with the body good biocompatibility, is had no side effect, and can be by the thorough degraded and absorbed of body in 3 months.
This invention is in medically application:
(1) hemostasis; (2) leak stopping (fistula); (3) promote tissue regeneration and wound healing; (4) prevent the tissue adhesion.
Can derive another kind of tourniquet bandage with product 2, that is:
3. absorbable fibre albumen tourniquet bandage
The hemostasis of absorbable fibre albumen is pasted and is added that conventional tourniquet bandage is absorbable fibre albumen tourniquet bandage of the present invention (being used for wound).
Absorbable fibre albumen tourniquet bandage of the present invention is better than the fibrin sealant tourniquet bandage of above-mentioned U.S. army development, earlier the hemostasis of absorbable fibre albumen is sticked in wound the common bandaging of reuse when using this invention.Can stop arterial hemorrhage rapidly like this, common binder not can with wound tissue's adhesion.Binder of the present invention can be used as treatment of war wound and emergency care of trauma at ordinary times, reduces mortality rate significantly.This tourniquet bandage still is blank in the application of China, and market prospect is very wide, and economic benefit and social benefit are huge.
Claims (3)
1, the production technology of medical collagen sponge, it is characterized in that: the tendon of carefully isolating the tendon → pulverizing tendon of the leg of pork → after will pulverizing is soaked in 0.1% the acetic acid solution, wherein add behind an amount of pepsin fully mixing → under room temperature (or 2 ℃～8 ℃), be saved to solution and reach the suitable viscosity after-filtration slagging-off → collagen solution of 98-99% and the glycerol of 1-2% are mixed, freezingly obtain 100～150 ℃ of spongy materials → place fixedly 60-120 minute down, obtain medical collagen sponge.
2, fibrin hemostasis plaster formula and production technology is characterized in that: with riboflavin (7～26 μ g/cm
2), Fibrinogen (4.3～6.7mg/cm of from Sanguis sus domestica, extracting
2) and thrombin (1.5～2.5IU/cm
2) lyophilized powder adds in the mortar fully porphyrize according to quantity, mix homogeneously → adding absolute ethyl alcohol and stirring becomes uniform suspension → pour in the stainless steel disc, concussion is affixed on particle uniform distribution → with the one side of collagen sponge and tries to get to the heart of a matter, and above-mentioned three kinds of granules promptly are attached on the collagen sponge → the evacuation drying → tailor into different size → encapsulation afterwards cobalt source radiation sterilization, the virus of going out.
3, the preparation of absorbable fibre albumen tourniquet bandage, the bleeding-stopping dressing that it is characterized in that contacting wound surface are that the 2 fibrins hemostasis of producing are pasted.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031267629A CN1552465A (en) | 2003-06-05 | 2003-06-05 | Fiber protein stopping bleeding paste |
| CN 200410033680 CN1234425C (en) | 2003-06-05 | 2004-04-16 | Method for preparing absorbent fibrin hemostatic plaster |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA031267629A CN1552465A (en) | 2003-06-05 | 2003-06-05 | Fiber protein stopping bleeding paste |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1552465A true CN1552465A (en) | 2004-12-08 |
Family
ID=34321997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA031267629A Pending CN1552465A (en) | 2003-06-05 | 2003-06-05 | Fiber protein stopping bleeding paste |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1552465A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100444902C (en) * | 2005-08-15 | 2008-12-24 | 上海其胜生物制剂有限公司 | Technique for preparing sponge produced from collagen |
| CN102131478A (en) * | 2008-06-26 | 2011-07-20 | 科研制药株式会社 | Agent for regenerating tympanic membrane or external auditory canal |
| CN102178975A (en) * | 2011-04-25 | 2011-09-14 | 福建南生科技有限公司 | Fibrous protein hemostatic patch and making method thereof |
| CN101874903B (en) * | 2009-11-20 | 2013-04-10 | 胡庆柳 | Method for preparing collagen artificial skin |
| CN104490760A (en) * | 2014-12-12 | 2015-04-08 | 广东医学院 | Preparation method and application of capsaicin-collagen sponge |
| CN104805165A (en) * | 2015-05-11 | 2015-07-29 | 江南大学 | Method for extracting water-insoluble collagen from thoracic aorta of pig |
| CN105056214A (en) * | 2015-06-30 | 2015-11-18 | 广西健宝石斛有限责任公司 | Dendrobium officinale haemostasis bandage |
| CN107080857A (en) * | 2017-03-29 | 2017-08-22 | 西南交通大学 | A kind of preparation method of the hemostatic material based on fibrin gel |
| CN112891610A (en) * | 2021-01-25 | 2021-06-04 | 上海利康瑞生物工程有限公司 | Pig-derived fibrin-electrostatic spinning nanofiber antibacterial hemostatic patch and preparation method thereof |
| CN112972755A (en) * | 2021-03-25 | 2021-06-18 | 哈尔滨瀚邦医疗科技有限公司 | Preparation method of biological hemostatic material based on porcine fibrinogen and thrombin |
| CN114177346A (en) * | 2021-12-24 | 2022-03-15 | 中国人民解放军军事科学院军事医学研究院 | Hemostatic composition, hemostatic patch and application thereof |
-
2003
- 2003-06-05 CN CNA031267629A patent/CN1552465A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100444902C (en) * | 2005-08-15 | 2008-12-24 | 上海其胜生物制剂有限公司 | Technique for preparing sponge produced from collagen |
| CN102131478A (en) * | 2008-06-26 | 2011-07-20 | 科研制药株式会社 | Agent for regenerating tympanic membrane or external auditory canal |
| CN101874903B (en) * | 2009-11-20 | 2013-04-10 | 胡庆柳 | Method for preparing collagen artificial skin |
| CN102178975A (en) * | 2011-04-25 | 2011-09-14 | 福建南生科技有限公司 | Fibrous protein hemostatic patch and making method thereof |
| CN102178975B (en) * | 2011-04-25 | 2013-10-23 | 福建南生科技有限公司 | Fibrous protein hemostatic patch and making method thereof |
| CN104490760A (en) * | 2014-12-12 | 2015-04-08 | 广东医学院 | Preparation method and application of capsaicin-collagen sponge |
| CN104805165A (en) * | 2015-05-11 | 2015-07-29 | 江南大学 | Method for extracting water-insoluble collagen from thoracic aorta of pig |
| CN104805165B (en) * | 2015-05-11 | 2018-02-23 | 江南大学 | The method that water-insoluble collagen is extracted in one boar aorta pectoralis |
| CN105056214A (en) * | 2015-06-30 | 2015-11-18 | 广西健宝石斛有限责任公司 | Dendrobium officinale haemostasis bandage |
| CN107080857A (en) * | 2017-03-29 | 2017-08-22 | 西南交通大学 | A kind of preparation method of the hemostatic material based on fibrin gel |
| CN112891610A (en) * | 2021-01-25 | 2021-06-04 | 上海利康瑞生物工程有限公司 | Pig-derived fibrin-electrostatic spinning nanofiber antibacterial hemostatic patch and preparation method thereof |
| CN112972755A (en) * | 2021-03-25 | 2021-06-18 | 哈尔滨瀚邦医疗科技有限公司 | Preparation method of biological hemostatic material based on porcine fibrinogen and thrombin |
| CN112972755B (en) * | 2021-03-25 | 2022-07-05 | 哈尔滨瀚邦医疗科技有限公司 | Preparation method of biological hemostatic material based on porcine fibrinogen and thrombin |
| CN114177346A (en) * | 2021-12-24 | 2022-03-15 | 中国人民解放军军事科学院军事医学研究院 | Hemostatic composition, hemostatic patch and application thereof |
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