CN100444902C - Technique for preparing sponge produced from collagen - Google Patents
Technique for preparing sponge produced from collagen Download PDFInfo
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- CN100444902C CN100444902C CNB2005100287591A CN200510028759A CN100444902C CN 100444902 C CN100444902 C CN 100444902C CN B2005100287591 A CNB2005100287591 A CN B2005100287591A CN 200510028759 A CN200510028759 A CN 200510028759A CN 100444902 C CN100444902 C CN 100444902C
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Abstract
A process for preparing the collagen sponge includes such steps as extracting collagen in acetic acid by digestion of pepsinum, filtering, taking supernatant, neutralizing by sodium hydroxide, salting out in sodium chloride solution, centrifugal separation of deposit, dissolving it in acetic acid, dialyzing by acetic acid and then by the solution of bisodium hydrogen phosphate, and freeze drying.
Description
Technical field
The present invention relates to a kind of preparation technology of collagen protein sponge, relate in particular to preparation a kind of purification freeze dried collagen protein sponge, can be used for hemostasis, the reparation of all kinds of burns, wound and surgical wound, and the filling of ulcer, residual cavity.
Background technology
Collagen protein is one group of extended family of being made up of multiple glycoprotein molecule, is the main protein composition of connective tissue, accounts for 25% of body total protein.Collagen protein is the structural protein of extracellular matrix, and it is supramolecular structure that collagen molecules is assembled in extracellular matrix.Collagen protein is a class protein family, the dissimilar collagen protein of having found now more than 16 kinds, I type, II type, III type and IV collagen type are to find by the Primary Structure Analysis on the protein level, and other then are to find by the similarity analysis of the collagen domain on nucleic acid level.Collagen protein is the same with other protein, also is made up of a-amino acid, and has 1～4 level structure and typical triple-helix structure.
The institute that each collagen type is distributed widely in body in a organized way in, but the distribution of each collagen type and content have very big-difference because of organizing difference.Thereby on purpose the tissue of required collagen molecules type is rich in selection, is considerable for obtaining a large amount of and highly purified collagen product.Though type i collagen albumen can prepare from many tissues or organ, the only raw material of preparation type i collagen albumen is skin, heel string and young organ skeleton.The fiber of heel string and skeleton nearly all is a type i collagen albumen, thereby has simplified the required program of purification type i collagen albumen greatly.Improve the purification efficiency of collagen protein, except selecting suitable tissue as the raw material, appropriate pre-treatment also is extremely important.The used non-collagen tissue that raw material contained, particularly fatty tissue etc. should fully be removed.At the normally used solvent of each collagen type of preparation is neutral salt solution and acid flux material, and the neutral salt solvent generally adopts NaCl solution, and acid flux material adopts acetic acid or citric acid soln more.No matter select which kind of method to extract collagen protein, may contain a large amount of impurity in the extract.General, in neutral or solution near neutral pH, natural collagen molecules high (3.5～4.0MNaCl) or the salinity of low (being lower than 0.15M NaCl) under can be by quantitative precipitation.In the diluted acid solvent, collagen molecules can be precipitated when 1.0～2.0M NaCl.So repeated precipitation is the prefered method that recovery and purification extract collagen molecules from neutral salt or diluted acid solvent.
The collagen protein sponge past that we produce is to be raw material with the cattle heel string, citric acid with 0.05mol/l is the acid soluble collagen that solvent extracts with pepsin digestion, adopt 10mol/l sodium hydrate regulator solution pH to neutral then, add the chlorination sodium salt analyse to collagen protein separate out fully, centrifugal collecting precipitation dissolves with citric acid, the citric acid dialysis is 2 days then, dialyse to pH with phosphate sodium chloride buffer and to reach more than 5.0 lyophilization in last 48 hours.The collagen protein protein content with this explained hereafter is low, salt content is high and product appearance after making lyophilizing is poor, quality hard and the wound surface adhesiveness is relatively poor, thereby easily coming off from wound surface influences haemostatic effect owing to separating out in a large number at collagen protein after the dialysis.
Summary of the invention
The objective of the invention is to invent that a kind of collagen protein sponge quality is pliable and tough, adhesiveness good, salt content is low, improve the preparation technology of the collagen protein sponge of clinical application effect.
For realizing above purpose, technical scheme of the present invention provides a kind of preparation technology of collagen protein sponge, it is characterized in that, and with acetic acid solvent, its technology is:
The first step. the preparation of cattle heel string fragment
Tissue mashing machine is put in the cattle heel string section of cleaning, add an amount of cold distilled water, start is smash the cattle heel string and is fragmentated, choose fascia, and with the cold distilled water washing, embathes 1-2 hour with normal saline, takes out to place spun silk to extract;
Second step. the extraction of collagen protein
1 part of cattle heel string fragment of extracting is placed glass jar, add 4～8 parts of 1～5% acetic acid, allow its swelling, 5～10 parts of 1～5% acetic acid that add sodium chloride-containing 10～15g again, and stir 15-30min with blender, add again and contain 2-10 and restrain 5～10 parts of pepsic 1～5% acetic acid, it is stirred;
After the digestion beginning, suitably add acetum again according to situation about digesting, continue digestion, the whole digestion process time is 2-5 days;
The 3rd step filtered
Digestive system is filtered twice with stainless steel filter, use the coarse filtration net filtration for the first time, remove thick precipitate, will carry out essence again through the collagen solution after the coarse filtration and filter;
The 4th step saltoutd
At first collagen solution is regulated pH to 7-9 with the NaOH solution of 5～12mol/L, in adjustment process, must fully stir, make whole solution even;
Add NaCl solution then, reach 2～3mol/l to sodium chloride concentration, the limit adds the sodium chloride limit stirs, adding finish the back stir treat that precipitation is separated out after, standing over night;
It is centrifugal to take out precipitation at last, removes supernatant, with the acetum washing that precipitates with 1%, and does 1-5 minute stop, extracts with spun silk; The number of times and the degree of washing can be judged according to sedimentary expansion and transparency, generally begin to be dissolved as standard with the collagen precipitation;
The dissolving of the 5th step
Precipitate is dissolved in 1% the acetum;
The dialysis of the 6th step
At first collagen solution is packed in the bag filter, the acetum with 0.1% is as extracellular fluid dialysis, and the volume of each extracellular fluid dialysis is 4～6 times that interior liquid amasss, changes once at least, dialyses two days in one day;
Then with bag filter outside with cold distilled water flushing be placed on dialyse in the sodium hydrogen phosphate extracellular fluid dialysis to the pH of collagen solution be more than 5.0, while each used extracellular fluid dialysis volume in dialysis procedure must be 4～6 times of interior liquid, and must change outer liquid every day;
The 7th step lyophilization
The collagen solution that dialysis is good injects the stainless steel disc that pyrogen-free lyophilizing is used with syringe, and every dish 15～20ml with freeze dryer lyophilization 24～48 hours, makes collagen protein sponge;
The 8th step packing
Open the dehumidifier of lyophilizing between offeing for sale before offeing for sale, make that the humidity when offeing for sale is lower than 40%, collagen sponge is packed in the bubble-cap, the lid aluminium-foil paper is packed in the aluminium foil bag then with the heat seal of bubble-cap heat-sealing machine, seals with sealing machine, carries out at last
60The Co irradiation sterilization;
The preparation technology of described collagen protein sponge is to carry out under 0～15 ℃ temperature conditions from the process of extracting dialysis.
The present invention adopts acetic acid to substitute citric acid, and acetic acid also is the good solvent that acid collagen extracts, and its extraction effect is better than citric acid; Salinity in the collagen sponge mainly be do not have in the dialysis procedure fully to be fallen by dialysis or extracellular fluid dialysis in the salt dialysis enter in the goods, therefore, reduce salt content and just can set about, the salinity in the reduction extracellular fluid dialysis from extracellular fluid dialysis; Moreover, the citric acid molecule amount is far longer than acetic acid, its dialysis speed is slower than acetic acid, and the dissolved collagen of citric acid easily produces the thread collagen of white when above and separates out dialysing to pH4.0, and the collagen of acetate dissolution does not generally have filament yet and separates out more than pH5.0.
Advantage of the present invention is:
(1) reduced the production cycle;
(2) reduce salt content in the collagen sponge, improved collagen content;
(3) improve the outward appearance of collagen sponge, increased pliability and adhesiveness, thereby improve anastalsis, improved clinical application effect.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment
The preparation technology of collagen protein sponge is to carry out under 0～15 ℃ temperature conditions from the process of extracting dialysis, and its technology is:
The first step. the preparation of cattle heel string fragment
Tissue mashing machine is put in the cattle heel string section of cleaning, add an amount of cold distilled water, start is smash the cattle heel string and is fragmentated, choose fascia, and with the cold distilled water washing, embathes 1-2 hour with normal saline, takes out to place spun silk to extract;
Second step. the extraction of collagen protein
The cattle heel string fragment of at first 100g being extracted places glass jar, adds 1% acetic acid 500ml, allows its swelling.The 1% acetic acid 500ml that adds sodium chloride-containing 10g again, and with more than the blender stirring 30min.Add again and contain among the pepsic 1% acetic acid 1000ml of 5 grams, it is stirred;
After the digestion beginning, suitably add acetum again according to situation about digesting then, continue digestion, the whole digestion process time is 3 days;
The 3rd step filtered
Digestive system is filtered twice with stainless steel filter, use the coarse filtration net filtration for the first time, remove thick precipitate, will carry out essence again through the collagen solution after the coarse filtration and filter;
The 4th step saltoutd
At first collagen solution is regulated pH to 8 with the NaOH solution of 10mol/L, in adjustment process, must fully stir, make whole solution even;
Add NaCl solution then, reach 2mol/l to sodium chloride concentration, the limit adds the sodium chloride limit stirs, adding finish the back stir treat that precipitation is separated out after, standing over night;
It is centrifugal to take out precipitation at last, removes supernatant, with the acetum washing that precipitates with 1%, and does 2 minutes stop, extracts with spun silk; The washing number of times and degree can judge according to sedimentary expansion and transparency,
Generally begin to be dissolved as standard with the collagen precipitation;
The dissolving of the 5th step
Precipitate is dissolved in 1% the acetum;
The dialysis of the 6th step
At first collagen solution is packed in the bag filter, the acetum with 0.1% is as extracellular fluid dialysis, and the volume of each extracellular fluid dialysis is 5 times that interior liquid amasss, changes once at least, dialyses two days in one day;
Then with bag filter outside with cold distilled water flushing be placed on dialyse in the sodium hydrogen phosphate extracellular fluid dialysis to the pH of collagen solution be more than 5.0, simultaneously in dialysis procedure at every turn used extracellular fluid dialysis volume must be 5 times of interior liquid, must change outer liquid every day;
The 7th step lyophilization
The collagen solution that dialysis is good injects the stainless steel disc that pyrogen-free lyophilizing is used with syringe, and every dish 15ml with freeze dryer lyophilization 36 hours, makes collagen protein sponge;
The 8th step packing
Open the dehumidifier of lyophilizing between offeing for sale before offeing for sale, make that the humidity when offeing for sale is lower than 40%, collagen sponge is packed in the bubble-cap, the lid aluminum foil paper, seals with sealing machine in the aluminum of packing into the then pool bag with the heat seal of bubble-cap heat-sealing machine, carries out at last
60The Co irradiation sterilization.
Following table is before the invention and the product quality comparison sheet that adopts the different process flow process to produce after the invention.
Collagen protein sponge quality comparison sheet before and after the invention
| Lot number | 040901 (before the invention) | 050102-1 (invention back) |
| Water absorption (doubly) | 16 | 51.89 |
| Protein content % | 62.5 | 82.88 |
| Hydroxyproline % | 12.96 | 13.93 |
| Moisture content | 10.77 | 10.55 |
| pH | 4.8 | 5 |
| Outward appearance | Not attractive in appearance, quality is hard, poor adhesion | Attractive in appearance, the pliability adhesiveness is good |
| Fat % | 0.57 | 0.59 |
| Ash % | 13.09 | 0.94 |
Claims (2)
1. the preparation technology of a collagen protein sponge is characterized in that, is solvent with acetic acid, and its technology is:
The first step. the preparation of cattle heel string fragment
Tissue mashing machine is put in the cattle heel string section of cleaning, add an amount of cold distilled water, start is smash the cattle heel string and is fragmentated, choose fascia, and with the cold distilled water washing, embathes 1-2 hour with normal saline, takes out to place spun silk to extract;
Second step. the extraction of collagen protein
1 part of cattle heel string fragment of extracting is placed glass jar, add 4～8 parts of 1～5% acetic acid, allow its swelling, 5～10 parts of 1～5% acetic acid that add sodium chloride-containing 10～15g again, and stir 15-30min with blender, add again and contain 2-10 and restrain 5～10 parts of pepsic 1～5% acetic acid, it is stirred;
After the digestion beginning, suitably add acetum again according to situation about digesting, continue digestion, the whole digestion process time is 2-5 days;
The 3rd step filtered
Digestive system is filtered twice with stainless steel filter, use the coarse filtration net filtration for the first time, remove thick precipitate, will carry out essence again through the collagen solution after the coarse filtration and filter;
The 4th step saltoutd
At first collagen solution is regulated pH to 7-9 with the NaOH solution of 5～12mol/L, in adjustment process, must fully stir, make whole solution even;
Add NaCl solution then, reach 2～3mol/l to sodium chloride concentration, the limit adds the sodium chloride limit stirs, adding finish the back stir treat that precipitation is separated out after, standing over night;
It is centrifugal to take out precipitation at last, removes supernatant, with the acetum washing that precipitates with 1%, and does 1-5 minute stop, extracts with spun silk; The number of times and the degree of washing can be judged according to sedimentary expansion and transparency, begin to be dissolved as standard with the collagen precipitation;
The dissolving of the 5th step
Precipitate is dissolved in 1% the acetum;
The dialysis of the 6th step
At first collagen solution is packed in the bag filter, the acetum with 0.1% is as extracellular fluid dialysis, and the volume of each extracellular fluid dialysis is 4～6 times that interior liquid amasss, changes once at least, dialyses two days in one day;
Then with bag filter outside with cold distilled water flushing be placed on dialyse in the sodium hydrogen phosphate extracellular fluid dialysis to the pH of collagen solution be more than 5.0, while each used extracellular fluid dialysis volume in dialysis procedure must be 4～6 times of interior liquid, and must change outer liquid every day;
The 7th step lyophilization
The collagen solution that dialysis is good injects the stainless steel disc that pyrogen-free lyophilizing is used with syringe, and every dish 15～20ml with freeze dryer lyophilization 24～48 hours, makes collagen protein sponge;
The 8th step packing
Open the dehumidifier of lyophilizing between offeing for sale before offeing for sale, make that the humidity when offeing for sale is lower than 40%, collagen sponge is packed in the bubble-cap, the lid aluminium-foil paper is packed in the aluminium foil bag then with the heat seal of bubble-cap heat-sealing machine, seals with sealing machine, carries out at last
60The Co irradiation sterilization.
2. the preparation technology of collagen protein sponge according to claim 1 is characterized in that, the preparation technology of described collagen protein sponge is to carry out under 0～15 ℃ temperature conditions from the process of extracting dialysis.
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| CNB2005100287591A CN100444902C (en) | 2005-08-15 | 2005-08-15 | Technique for preparing sponge produced from collagen |
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| CN100444902C true CN100444902C (en) | 2008-12-24 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030036636A1 (en) * | 2001-08-17 | 2003-02-20 | National Cheng Kung University | Process for preparing porous collagen matrix from connective tissue |
| CN1406632A (en) * | 2001-09-10 | 2003-04-02 | 中国人民解放军军事医学科学院卫生装备研究所 | Collagen based composite sponge and production thereof |
| US20040167318A1 (en) * | 2001-06-14 | 2004-08-26 | Bhanumathy Manickavasagam | Process for extracting collagen from marine invertebrates |
| CN1552465A (en) * | 2003-06-05 | 2004-12-08 | 胡庆柳 | Fiber protein stopping bleeding paste |
| CN1179755C (en) * | 2001-11-09 | 2004-12-15 | 武继民 | Medical collagen sponge and its prepn |
-
2005
- 2005-08-15 CN CNB2005100287591A patent/CN100444902C/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040167318A1 (en) * | 2001-06-14 | 2004-08-26 | Bhanumathy Manickavasagam | Process for extracting collagen from marine invertebrates |
| US20030036636A1 (en) * | 2001-08-17 | 2003-02-20 | National Cheng Kung University | Process for preparing porous collagen matrix from connective tissue |
| CN1406632A (en) * | 2001-09-10 | 2003-04-02 | 中国人民解放军军事医学科学院卫生装备研究所 | Collagen based composite sponge and production thereof |
| CN1179755C (en) * | 2001-11-09 | 2004-12-15 | 武继民 | Medical collagen sponge and its prepn |
| CN1552465A (en) * | 2003-06-05 | 2004-12-08 | 胡庆柳 | Fiber protein stopping bleeding paste |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103007336A (en) * | 2013-01-14 | 2013-04-03 | 福建省博特生物科技有限公司 | Fish skin collagen based composite sponge and preparation method thereof |
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