CN1546104A - Hypertension treating sage whole plant products and preparation - Google Patents
Hypertension treating sage whole plant products and preparation Download PDFInfo
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- CN1546104A CN1546104A CNA2003101115038A CN200310111503A CN1546104A CN 1546104 A CN1546104 A CN 1546104A CN A2003101115038 A CNA2003101115038 A CN A2003101115038A CN 200310111503 A CN200310111503 A CN 200310111503A CN 1546104 A CN1546104 A CN 1546104A
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- herba hyperici
- hyperici japonici
- big herba
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Abstract
The invention discloses a Datianjihuang extract for lowering blood pressure and the process for preparation, wherein low-temperature extraction membrane separation technique and freeze drying process are employed for the manufacture of Datianjihuang, which comprises, watering and boiling, supersonic extraction, filtering, collecting portions whose molecular weight is greater than 1000, reverse osmosis concentration and freeze drying to obtain the end product.
Description
Technical field:
The present invention relates to a kind of antihypertensive drug and production method thereof.
Background technology:
Big Herba Hyperici Japonici (formal name used at school: be traditional Chinese herbal medicine Lysimachia fortunei Maxim), have heat-clearing and toxic substances removing, antiinflammatory diuretic effect, tradition is used for the treatment of jaundice, hepatitis etc., and process technology is the method by traditional decocting in water diet.
Summary of the invention:
The objective of the invention is to determine the blood pressure reduction effect that big Herba Hyperici Japonici had, and its blood pressure lowering effective part group has been separated, made antihypertensive product.
Main technical schemes of the present invention adopts the extract at low temperature membrane separation technique exactly, and freeze drying process carries out the production and processing of big Herba Hyperici Japonici.Being about to big Herba Hyperici Japonici adds decocting in water (temperature is below 60 ℃) or uses supersound extraction, filter, filtrate is filtered with macroporous adsorbent resin (D101) post, the membrane ultrafiltration of filtrate reuse microfiltration, 1000 molecular weight, collect molecular weight greater than 1000 positions, reverse osmosis concentration, lyophilization (or the cryogenic thermostat vacuum drying is less than 65 ℃) are promptly.Molecular weight has significant hypotensive activity greater than 1000 big Herba Hyperici Japonici effective part group.
1, extraction and separation process flow process:
Big Herba Hyperici Japonici-decocting in water (or supersound extraction, bright material is defibrination directly, keeps from heat to destroy effective ingredient)-coarse filtration-Filter column (filler is the D101 macroporous adsorbent resin) filtration-high speed centrifuge filtration (or plate-and-frame filtration)-microfiltration-ultrafiltration and concentration (or nanofiltration)-lyophilization (or low-temperature vacuum drying is promptly).
2, technological operation main points:
(1) decocting in water (or supersound extraction): because effective ingredient destructible under high temperature decocting in water condition of big Herba Hyperici Japonici, so answer temperature control about about 80 ℃ (being preferably in below 60 ℃), decocting in water twice, 2～2.5 hours for the first time, 1.5～2.0 hours for the second time during decocting in water.Amount of water is 10～20 times (liquid level soaked medicinal herbs and is as the criterion).
(2) filter:
1. coarse filtration: filter for the first time, filtering residue adds water and carries out extracting the second time after-filtration, filters the back medicinal residues for the second time and removes, and first, second time coarse filtration liquid merges treats next step processing.
2. Filter column: will go up step coarse filtration liquid and handle, and remove impurity component through the filter post.Filtrate is waited until next step processing (annotate: the filter post can be isolated glycosides composition with 70%E OH eluant solution).
3. centrifuge filters: on go on foot filtrate through the high speed centrifuge filtering, in order to avoid the water-insoluble granule influences microfiltration.
(3) microfiltration: by microfiltration 0.01 μ m～0.05 μ m, can make product reach the sanitary index standard removal of bacteria, improve ultrasiltrated rate simultaneously.
(4) ultrafiltration (or reverse osmosis concentration): by selecting the film of different molecular weight for use, the blood pressure lowering effective ingredient is carried out exquisiteness separate, antihypertensive compositions is molecular weight 〉=1000 parts.
(5) drying:
1. lyophilization: in order to keep the biological activity of its former medicine, molecular weight 〉=1000 parts are carried out lyophilization, exsiccant product promptly can be used for producing end products such as tablet, capsule, buccal tablet, oral liquid.
2. cryogenic thermostat vacuum drying: because freeze drying equipment is expensive, generally can adopt vacuum drying, baking temperature is controlled at below 65 ℃.Dry products promptly is used to produce end product.
Advantage of the present invention or good effect:
The present invention is the effective part group of having found to have remarkable hypotensive effect from big Herba Hyperici Japonici first, its effect hypotensive effect and Western medicine nifedipine commonly used are with imitating under the condition of suitable dosage, open up the new application of this medicine, broken through the inapparent difficulty of Chinese hypotensor curative effect in the market.Simultaneously, technology of the present invention has adopted advanced membrane separation technique, is solvent with water in the course of processing, and advanced free of contamination advantage possesses skills.More meaningfully, effective blood pressure lowering part group that the present invention finds, its molecular weight is bigger, is the more special macromolecular compound of a class, sees pharmacological effect laboratory report (" research of Chinese medicine Y extract hypotensive activity ") for details, has significant learning value.
The specific embodiment:
Embodiment 1:
Big Herba Hyperici Japonici 150kg soaked raw material, heating extraction 2 times, and each 2 hours, 60 ℃ of water temperatures were filtered.Filtrate is put cold, and (filler is D101, and 150kg), effluent is crossed microfiltration (0.01 μ m～0.05 μ m) to cross chromatography filter post, micro-filtrate ultrafiltration (membrane molecule amount 〉=1000) concentrates, and the concentrated solution lyophilization is refrigerated to-45 ℃ earlier, distillation then, 35 ℃ of sublimation temperatures get dry powder 1.46kg.
Embodiment 2:
Big Herba Hyperici Japonici 101kg adds the hydro-thermal lixiviate, extracts 2 times, each 2 hours, 60 ℃ of water temperatures were filtered, filtrate adds cold water and is cooled to 30 ℃, and (filler is D101,150kg) to cross chromatography filter post, effluent is crossed microfiltration (0.01 μ m～0.05 μ m) micro-filtrate ultrafiltration (membrane molecule amount 〉=1000) and is concentrated, the concentrated solution lyophilization is refrigerated to-45 ℃ earlier, then distillation, 38 ℃ of sublimation temperatures get dry powder 0.9kg.
The dry powder semi-finished product are carried out tabletting again or fill capsule or the modulation oral liquid, get final finished.
Isolated effective blood pressure lowering part group chemical reaction feature is as follows:
A: get the 1ml aqueous extract, add 1～2 of 1% ferric chloride alcoholic solution.Mulberry reaction (tannin reaction) is arranged.
B: get the 1ml aqueous extract, add 4～5 of the new alkaline cupric tartrate reagent of preparing, drop in the boiling water and heated 5 minutes, produce reddish brown precipitation (polysaccharide reaction, reducing sugar reaction).
C: get 1ml cold water extraction liquid, add 2～3 of 0.2% ninhydrin solutions, shake up, heating is 5 minutes in boiling water, after the cooling, and no color reaction (not containing aminoacid, polypeptide or protein-based).
D: this product is easy to water-soluble, is insoluble to organic solvents such as ethanol, chloroform, acetone, petroleum ether.
Claims (3)
1, a kind of big Herba Hyperici Japonici antihypertensive product is characterized in that: molecular weight has significant hypotensive activity greater than 1000 big Herba Hyperici Japonici effective part group.
2, a kind of production technology of big Herba Hyperici Japonici antihypertensive product adopts decocting in water, extraction and filtration, concentrates, and drying means is characterized in that:
Filtrate is filtered with macroporous adsorptive resins, crosses microfiltration, with the membrane ultrafiltration of 1000 molecular weight.
3, the production technology of big Herba Hyperici Japonici antihypertensive product according to claim 2 is characterized in that: filtrate is filtered with macroporous adsorbent resin D101 chromatographic column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310111503 CN1273148C (en) | 2003-11-28 | 2003-11-28 | Hypertension treating sage whole plant products and preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310111503 CN1273148C (en) | 2003-11-28 | 2003-11-28 | Hypertension treating sage whole plant products and preparation |
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| Publication Number | Publication Date |
|---|---|
| CN1546104A true CN1546104A (en) | 2004-11-17 |
| CN1273148C CN1273148C (en) | 2006-09-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200310111503 Expired - Fee Related CN1273148C (en) | 2003-11-28 | 2003-11-28 | Hypertension treating sage whole plant products and preparation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1320891C (en) * | 2004-12-24 | 2007-06-13 | 中山大学 | Extractive of Japanese St.Johnswort and preparation method and application |
| CN100434148C (en) * | 2004-12-08 | 2008-11-19 | 张玲 | Process for separating and purifying organic compound by macroporous absorption resin-reverse osmosis membrane |
| CN101028317B (en) * | 2007-03-30 | 2010-12-22 | 成都南山药业有限公司 | Use of hypericum japonicum in preparation of medicine against nephritis and renal insufficiency |
| CN104352815A (en) * | 2014-11-11 | 2015-02-18 | 成都华熹科技有限公司 | Lysimachia fortunei-containing traditional Chinese medicine composition for lowering blood pressure |
| CN104383012A (en) * | 2014-11-11 | 2015-03-04 | 成都华熹科技有限公司 | Traditional Chinese medicine composition containing phoenix tree leaf for reducing blood pressure |
-
2003
- 2003-11-28 CN CN 200310111503 patent/CN1273148C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434148C (en) * | 2004-12-08 | 2008-11-19 | 张玲 | Process for separating and purifying organic compound by macroporous absorption resin-reverse osmosis membrane |
| CN1320891C (en) * | 2004-12-24 | 2007-06-13 | 中山大学 | Extractive of Japanese St.Johnswort and preparation method and application |
| CN101028317B (en) * | 2007-03-30 | 2010-12-22 | 成都南山药业有限公司 | Use of hypericum japonicum in preparation of medicine against nephritis and renal insufficiency |
| CN104352815A (en) * | 2014-11-11 | 2015-02-18 | 成都华熹科技有限公司 | Lysimachia fortunei-containing traditional Chinese medicine composition for lowering blood pressure |
| CN104383012A (en) * | 2014-11-11 | 2015-03-04 | 成都华熹科技有限公司 | Traditional Chinese medicine composition containing phoenix tree leaf for reducing blood pressure |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1273148C (en) | 2006-09-06 |
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Granted publication date: 20060906 Termination date: 20091228 |