CN1536361A - 以精子分离、胚胎性别鉴定为基础的家畜性别控制方法 - Google Patents
以精子分离、胚胎性别鉴定为基础的家畜性别控制方法 Download PDFInfo
- Publication number
- CN1536361A CN1536361A CNA031094260A CN03109426A CN1536361A CN 1536361 A CN1536361 A CN 1536361A CN A031094260 A CNA031094260 A CN A031094260A CN 03109426 A CN03109426 A CN 03109426A CN 1536361 A CN1536361 A CN 1536361A
- Authority
- CN
- China
- Prior art keywords
- embryo
- sperm
- sex
- milliliter
- minutes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000000926 separation method Methods 0.000 title claims abstract description 9
- 244000144972 livestock Species 0.000 title claims description 3
- 238000007710 freezing Methods 0.000 claims abstract description 12
- 230000008014 freezing Effects 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 9
- 238000005520 cutting process Methods 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 7
- 239000000523 sample Substances 0.000 claims description 34
- 210000000582 semen Anatomy 0.000 claims description 25
- 210000004027 cell Anatomy 0.000 claims description 16
- 239000008363 phosphate buffer Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241001465754 Metazoa Species 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 230000004720 fertilization Effects 0.000 claims description 8
- 238000012546 transfer Methods 0.000 claims description 8
- 238000005516 engineering process Methods 0.000 claims description 7
- 238000004321 preservation Methods 0.000 claims description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 6
- 210000002308 embryonic cell Anatomy 0.000 claims description 6
- 210000004681 ovum Anatomy 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 5
- 238000000338 in vitro Methods 0.000 claims description 5
- 238000001962 electrophoresis Methods 0.000 claims description 4
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical group C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 claims description 3
- 229920000936 Agarose Polymers 0.000 claims description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000013016 damping Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000004043 dyeing Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 230000000050 nutritive effect Effects 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 239000000376 reactant Substances 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- 102000035195 Peptidases Human genes 0.000 claims description 2
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 235000019833 protease Nutrition 0.000 claims description 2
- 108090000942 Lactalbumin Proteins 0.000 claims 1
- 241000283690 Bos taurus Species 0.000 abstract description 3
- 238000005070 sampling Methods 0.000 abstract 2
- 238000003860 storage Methods 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 1
- 238000007689 inspection Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000019100 sperm motility Effects 0.000 description 3
- 210000002593 Y chromosome Anatomy 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 241000283725 Bos Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03109426 CN1232231C (zh) | 2003-04-10 | 2003-04-10 | 以精子分离、胚胎性别鉴定为基础的家畜性别控制方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03109426 CN1232231C (zh) | 2003-04-10 | 2003-04-10 | 以精子分离、胚胎性别鉴定为基础的家畜性别控制方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1536361A true CN1536361A (zh) | 2004-10-13 |
CN1232231C CN1232231C (zh) | 2005-12-21 |
Family
ID=34319327
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 03109426 Expired - Lifetime CN1232231C (zh) | 2003-04-10 | 2003-04-10 | 以精子分离、胚胎性别鉴定为基础的家畜性别控制方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1232231C (zh) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100478675C (zh) * | 2005-02-07 | 2009-04-15 | 北京大学 | 幼鱼或鱼受精卵的dna鉴定性别的方法 |
CN101322489B (zh) * | 2008-08-01 | 2011-11-30 | 内蒙古蒙牛繁育生物技术有限公司 | 一种鹿x/y精子分离冷冻精液及其生产方法和应用 |
CN103858821A (zh) * | 2014-03-25 | 2014-06-18 | 西北农林科技大学 | 一种增加奶山羊x精子比率的饲喂方法及营养添加剂 |
CN108531445A (zh) * | 2017-03-01 | 2018-09-14 | 山下直树 | 人类y精子的筛选方法 |
US10208345B2 (en) | 2000-05-09 | 2019-02-19 | Xy, Llc | Method for producing high purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa |
US11230695B2 (en) | 2002-09-13 | 2022-01-25 | Xy, Llc | Sperm cell processing and preservation systems |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
DK2305173T3 (en) | 2003-03-28 | 2016-08-22 | Inguran Llc | Apparatus and method for providing sorted particles |
-
2003
- 2003-04-10 CN CN 03109426 patent/CN1232231C/zh not_active Expired - Lifetime
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10208345B2 (en) | 2000-05-09 | 2019-02-19 | Xy, Llc | Method for producing high purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa |
US11230695B2 (en) | 2002-09-13 | 2022-01-25 | Xy, Llc | Sperm cell processing and preservation systems |
CN100478675C (zh) * | 2005-02-07 | 2009-04-15 | 北京大学 | 幼鱼或鱼受精卵的dna鉴定性别的方法 |
CN101322489B (zh) * | 2008-08-01 | 2011-11-30 | 内蒙古蒙牛繁育生物技术有限公司 | 一种鹿x/y精子分离冷冻精液及其生产方法和应用 |
CN103858821A (zh) * | 2014-03-25 | 2014-06-18 | 西北农林科技大学 | 一种增加奶山羊x精子比率的饲喂方法及营养添加剂 |
CN103858821B (zh) * | 2014-03-25 | 2015-08-12 | 西北农林科技大学 | 一种增加奶山羊x精子比率的饲喂方法及营养添加剂 |
CN108531445A (zh) * | 2017-03-01 | 2018-09-14 | 山下直树 | 人类y精子的筛选方法 |
Also Published As
Publication number | Publication date |
---|---|
CN1232231C (zh) | 2005-12-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Thundathil et al. | Fertility management of bulls to improve beef cattle productivity | |
CN105506086B (zh) | 鸡受精持续时间性状相关的snp分子标记及其应用 | |
CN101062058A (zh) | 细管高密度冷冻猪精液的方法及其产品 | |
CN1674780A (zh) | 低压精子分离系统 | |
CN1232231C (zh) | 以精子分离、胚胎性别鉴定为基础的家畜性别控制方法 | |
CN1284849C (zh) | 一种分离哺乳动物xy精子的方法 | |
CN1990879A (zh) | 利用巢式pcr进行水牛胚胎性别鉴定的方法 | |
JP7142842B2 (ja) | 哺乳動物精子の分離方法、人工授精方法及び体外受精方法 | |
CN101899526B (zh) | 一种山羊产羔性状的分子标记选择方法 | |
Hansen | Some challenges and unrealized opportunities toward widespread use of the in vitro-produced embryo in cattle production | |
CN102605064A (zh) | 一种良种奶山羊多基因聚合的选育方法 | |
CN101906480A (zh) | 利用神经内分泌因子基因选择山羊产羔性状的分子标记方法 | |
CN101845507B (zh) | 检测山羊促性腺激素释放激素和生长分化因子9的碱基突变多态性的方法 | |
RU2456957C2 (ru) | Способ определения стрессовой чувствительности у свиноматок | |
CN103468819A (zh) | 一种利用双基因聚合效应选育奶山羊产奶性状的方法 | |
CN114085809B (zh) | 一种分离x精子和y精子的方法 | |
Saadi et al. | Modern reproductive technologies and breed improvement. | |
CN101845506A (zh) | 一种良种奶山羊多基因聚合的选育方法 | |
Herbicht et al. | Evaluation of a novel microfluidic chip-like device for purifying bovine frozen-thawed semen for in vitro fertilization | |
CN113564239B (zh) | 牛早期胚胎性别鉴定的方法 | |
Oliveira et al. | Imputation accuracy for genomic selection using embryo biopsy samples in Gir | |
Rocha | Phenotypic and genomic analysis of the number of oocytes and embryos of the Gir breed | |
CN113699256B (zh) | 一种快速鉴定马口鱼及锦鲤雌核发育子代的功能标记及其应用 | |
CN101928774B (zh) | 检测山羊生长激素基因编码区5个碱基突变多态性的方法 | |
CN101956009A (zh) | 检测猪窝产活仔数性状的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Owner name: NEI MENGGU MENGNIU BREEDING BIOLOGY TECHNOLOGYCO. Free format text: FORMER OWNER: LI XIHE Effective date: 20061027 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20061027 Address after: 011517 Hohhot city Helingeer County Inner Mongolia Shengle economic Park Patentee after: Inner Mongolia Mengniu Breeding Biotechnology Co.,Ltd. Address before: 300061 Tianjin city Hexi District heiniucheng road Yicheng District 9-5-601 Patentee before: Li Xihe |
|
C56 | Change in the name or address of the patentee | ||
CP01 | Change in the name or title of a patent holder |
Address after: 011517 Hohhot city Helingeer County Inner Mongolia Shengle economic Park Patentee after: MENGNIU REPRODUCTIVE BILTECHNOLOGY Co.,Ltd. Address before: 011517 Hohhot city Helingeer County Inner Mongolia Shengle economic Park Patentee before: Inner Mongolia Mengniu Breeding Biotechnology Co.,Ltd. |
|
C56 | Change in the name or address of the patentee |
Owner name: INNER MONGOLIA SEXING REPRODUCTIVE BIOTECHNOLOGY C Free format text: FORMER NAME: INNER MONGOLIA MENGNIU REPRODUCTIVE BIOTECHNOLOGY CO., LTD. |
|
CP01 | Change in the name or title of a patent holder |
Address after: 011517 Hohhot city Helingeer County Inner Mongolia Shengle economic Park Patentee after: INNER MONGOLIA SKXING REPRODUCTIVE BIOTCHNOLOGY Co.,Ltd. Address before: 011517 Hohhot city Helingeer County Inner Mongolia Shengle economic Park Patentee before: MENGNIU REPRODUCTIVE BILTECHNOLOGY Co.,Ltd. |
|
C56 | Change in the name or address of the patentee |
Owner name: INNER MONGOLIA SKXING REPRODUCTIVE BIOTECHNOLOGY ( Free format text: FORMER NAME: INNER MONGOLIA SEXING REPRODUCTIVE BIOTECHNOLOGY CO., LTD. |
|
CP03 | Change of name, title or address |
Address after: 011517 the Inner Mongolia Autonomous Region Helingeer County of Hohhot city Shengle economic Park gathering service area management committee building letter South 4 building room 4422 Patentee after: INNER MONGOLIA SK-XING BREEDING BIOTECHNOLOGY (Group) Co.,Ltd. Address before: 011517 the Inner Mongolia Autonomous Region Helingeer County of Hohhot city Shengle Economic Zone Patentee before: INNER MONGOLIA SKXING REPRODUCTIVE BIOTCHNOLOGY Co.,Ltd. |
|
CX01 | Expiry of patent term | ||
CX01 | Expiry of patent term |
Granted publication date: 20051221 |