CN1531876A

CN1531876A - Flourescent serial drink

Info

Publication number: CN1531876A
Application number: CNA031196381A
Authority: CN
Inventors: 力周; 周力
Original assignee: Individual
Current assignee: Individual
Priority date: 2003-03-18
Filing date: 2003-03-18
Publication date: 2004-09-29

Abstract

The serial drinks capable of glowing with colored fluorescence include fluorescent beer, fluorescent white spirit, fluorescent fruit spirit, fluorescent beverage, fluorescent frozen sucker, etc. By means of biological fluorescent mark technology, green fluorescin or composition containing its gene is applied into various drinks, so that the drinks possess green fluorescin gene and characteristic originated from jelly fish and exhibit fluorescent effect. The technological scheme includes the synthesis, expression and cloning of green fluorescin during the beverage brewing process and making fluorescent agent or ice block with fluorescin gene and fluorescent effect for adding into various drinks. The present invention makes drinks vivid, and has increased protein content and no side effect.

Description

The fluorescence beverage series

Technical field: this invention relates to the daily all kinds of common drink of drinking of people.Biotechnologys such as its employing bio-science field green fluorescent protein mark act on common drink, make drink have the ocean of coming from jellyfish medium green look fluorescence protein gene and feature, are a kind of beverage series that can send fluorescent effect of all kinds.Comprise fluorescence wine, fluorescence beverage, fluorescence ice cube etc.

Background technology: as everybody knows, all kinds of drinks such as existing liquor, fruit wine, beer, soda, all present natural colour separately, beverage itself can not send dynamic effects such as fluorescence, and drink self does not contain yet and comes from halobiontic green fluorescent protein (GFP).People when using these drinks, except satisfying with the utility function that all kinds of drinks are possessed self its basic need, the person's that also wishes to express the drink whereby specific mood.And original drink for the happy mood of giving full play to and embody people, is out of reach.

Among the present invention, in modern bio-science field, be mainly used in green fluorescent protein (GFP) or its optimization mutant (fusion) of gene expression label, be applied in all kinds of drink new product developments.Utilization fluorescence labeling technology, with green fluorescent protein (GFP) or fluorescin optimize that mutant (fusion) acts on that all kinds of common drinks are brewageed, in processing or the use, under the prerequisite that does not change the quality of original drink own, mouthfeel, function, make this drink under night or ultraviolet light, can send bright fluorescence of all kinds, have strong dynamic effect, can fully show cheerful and light-hearted, celebrating atmosphere, thereby transfer people's happy mood.

(Green-fluorescentprotein GFP), is the novel reporter molecules that this century, the seventies was found in jellyfish to the green fluorescent protein that the present invention relates to and use, and it can and send fluorescence at multiple biological expression in vivo such as animal, plant, microorganisms.The GFP of purifying is to have 238 amino acid, and molecular weight is the monomeric protein of 26888Da.The green fluorescent protein of wild type (wtGFP) can send green fluorescence under ultraviolet excitation, transform through the specific amino acids fixed point to its ray structure territory, can also produce polytype mutant.Present GFP can be excited in the wavelength of visible light scope (uptake zone red shift), and the original strong hundreds of times of luminous strength ratio, yellow fluorescence protein also occurred, blue fluorescent protein or the like.At present, GFP has been widely used in many-sides such as the location of albumen in the report, cell dynamic research, living cells of gene expression and westernbloting detection, around the correlation technique of green fluorescent protein (GFP), comparatively conventional biological study means have been become.

In recent years, China also obtains a lot of scientific achievements in this field.As calendar year 2001, the method that Xiamen University's pcr amplification that life science adopts and southern screening by hybridization combine, from the large-scale multitube jellyfish in waters, Xiamen, be separated to new green fluorescence protein gene gfpxm, and in Escherichia coli, express, the acquisition national patent (＜notification number〉1376678), or the like.(See Attachment 1)

From the sixties in this century, both at home and abroad on the basis of fluorescence labeling technology, omnibearing big quantity research, the very complete maturation of every technology have been carried out in the gene optimization of green fluorescent protein (GFP), clone, group training, purifying, expression etc.Existing green fluorescent protein correlation technique can satisfy the present invention for the synthetic primary demand of common drink fluorescence.

Summary of the invention: the main technical problem to be solved in the present invention is, keeping under the constant prerequisite of the original quality of drink, adds to come from halobiontic green fluorescent protein (GFP) in all kinds of common drinks, sends versicolor fluorescent effect thereby it is had.

Technical scheme that is to say green fluorescent protein (GFP) or the synthetic (fluorescer, ice cube) that contains this gene are applied in the various common drinks, thereby makes common drink present the scheme of fluorescent effect.

The content of the technical program: in the production or process of consumption of all kinds of drinks, the suitable stage of selection and definite luciferase expression, main by conventional biotechnology means such as pcr amplification, southern screening by hybridization and biological green fluorescin signs, to drink brewing materials or relevant bio-carrier, implement green fluorescent protein (GFP) construction expression, brewage synthetic etc., thereby make common drink obtain green fluorescent protein (GFP) gene and feature, and possess its fluorescent effect with this.

Technical scheme has two:

1. screen and adopt green fluorescent protein (GFP) or its to optimize mutant gene, in the drink brewing process, the semi-finished product raw material that are about to corresponding drink are expressed, are synthesized, are cloned, nurture the raw material of (GFP) gene that has green fluorescent protein, and participate in the brewing process of drink directly with this;

2. cultivation thalline or the related organisms purification or synthetic to containing green fluorescent protein (GFP) or its optimization mutant, make and can be used in the various common drinks, contain green fluorescence protein gene and have the fluorescer or the fluorescence ice cube of fluorescent effect, be applied to the production or the use of drink.

The background technology of fluorescin required for the present invention relates to three contents:

1. screening or rebuild is applicable to that green fluorescent protein of the present invention (GFP) or its optimize mutant (fusion).(case sees Appendix two);

2. select or make up and be fit to green fluorescent protein cloning vector of the present invention (as thalline), and cloned and cultivate (case sees Appendix three); Or green fluorescent protein (GFP) gene expressed in the drink raw material and synthetic, participate in the drink brewing process directly, make its drink have new fluorescent effect.

3. will be purified at the green fluorescent protein in the cloning vector of being cultivated (GFP), fusion or fluorescin object, be synthesized, make and contain green fluorescence protein gene (GFP) and can produce the fluorescer or the fluorescence ice cube of fluorescent effect, be used for the synthetic of all kinds of fluorescence drinks or brewage.

The present invention has broken through the inherent characteristic of traditional drink, keeping under the constant prerequisite of original drink quality, make it present multicoloured fluorescent effect, thereby make static in the past all kinds of drinks shine great innervation and vigor, having satisfied people follows the fashion, thirst for the domestic demand of passion, be particluarly suitable for public place of entertainment and celebrate joyously in one's power under the occasion and enjoy.Simultaneously, because fluorescin GFP is that gene or the gene optimization that extracts in marine organisms forms, itself is just in biological chain, therefore fluorescence drink of the present invention does not have any side effect, also can not change quality, mouthfeel, the color and luster of original drink, and because the protein function that fluorescin itself is had is compared with former drink, the fluorescence drink has also increased its nutritive effect to a certain extent.

The specific embodiment: the present invention preferentially adopts dual mode to make the fluorescence drink in concrete enforcement.

1. the fluorescer that contains green fluorescent protein (GFP) or its optimization mutant (synthetic fusion) gene that will synthesize is melted in the water, makes to be suitable for putting into the small dimension fluorescence ice cube that drinking vessel is drunk.When drinking drink, add in the drink Normal juice, can send corresponding fluorescent effect.

2. be main body fluorescence drink with drinks such as beer.In the brewing process of drinks such as beer, its brewing materials is imposed the gene expression that green fluorescent protein (GFP) or its are optimized mutant (synthetic fusion), thereby brew forms all kinds of fluorescence wine such as fluorescence beer.

Claims

1, a kind of adding green fluorescent protein (GFP) characteristic can be sent the beverage series of fluorescent effect of all kinds.It is characterized in that: with green fluorescent protein (GFP) gene action in all kinds of common drinks, thereby make this drink obtain the luciferase expression effect of green fluorescent protein (GFP).

2, described according to claim 1, by means such as biotechnology such as pcr amplification, southern screening by hybridization and biological green fluorescin signs, make this beverage series have the gene and the gene expression characteristics of green fluorescent protein (GFP) or its optimization mutant (fusion).

3, described according to claim 1, different phase in the drink manufacturing, mainly be biotechnologys such as application fluorescence labeling, through to green fluorescent protein (GFP) and optimize mutant brewing materials or associated biomolecule carrier construction expression, clone organize training, brewage technical schemes such as synthetic, make common drink acquisition fluorescent effect.Therefore, can directly brewage all kinds of fluorescence drinks, the drink that also can make the fluorescence additive of drink or contain green fluorescence protein gene adds ice cube, uses in the drink post-production or when drinking.

4, described according to claim 1, the present invention is same technical scheme to all kinds of drinks employings, has same technical characterictic, therefore, no matter to alcoholic beverages such as beer, fruit wine, liquor, still to all kinds of non-alcohol drinks such as soda, fruit drink, mineral water, cold drink products, ice cubes, perhaps to solid-state drinks such as cold drink products, ice cubes, all can make green fluorescent protein (GFP) gene expression also make by technical scheme of the present invention.Product of the present invention is serial fluorescence drink.