The bioamnion preservation treatment method
Technical field
The present invention relates to a kind of preservation treatment method of medicine equipment dressing amnion raw material.
Background technology
Amnion is because self exclusive characteristic receives the Chinese scholars concern, amnion is meant human placenta's basement membrane layer, it is the collagen tissue layer of no blood vessel, no cell structure, be rich in IV, collagen type v, basic fibroblast growth factor (BFGF), stem cell factor (HGF), the differentiation, the propagation that epithelial cell are divided a word with a hyphen at the end of a line grow into, promote epithelium play an important role in the epithelium wound healing.Amnion contains various albumen inhibiting factors, brings into play antiinflammatory action by suppressing the corresponding protein enzyme.Also contain simultaneously and suppress cytokine-expressing and regulate Apoptosis composition TGF-β
1, can avoid the collagenous fibres hyperplasia, the prevention adhesion.Reach a tissue repair and a purpose that table is rebuild and prevented to recur by above-mentioned mechanism.As a kind of new biomaterial, be widely used in the supplemental treatment of burn wound and ophthalmology disease in recent years both at home and abroad clinically, its curative effect highly significant.From domestic and foreign literature data report, used clinically amnion is fresh amnion, or the amnion that adopts profound hypothermia (80 ℃) to preserve.Fresh amnion often can not guarantee urgent clinical needs, and the amnion holding time that low temperature is preserved is short, maximum 1~2 month of its holding time, and transportation is inconvenient.
Summary of the invention
The objective of the invention is to: at the problems referred to above, provide that a kind of processing method is easy, long shelf-life, the bioamnion preservation treatment method that can meet clinical needs at any time.
Bioamnion preservation treatment method of the present invention is: with the amnion and the chorion blunt separation of placenta; clean; the amnion cuticular cellulose that obtains with the holder of nitrocellulose filter paper below last, with the glycerine protection of 0.1-10%; adopt freeze drying process; material is put 0 ℃~-30 ℃ pre-freezes in operating room 1~2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃~-45 ℃; vacuum cooling drying 3~8 hours, sealing, sterilization, dry shady and cool place preserve.
Bioamnion preservation treatment method of the present invention through after the freeze drying, makes the holding time of amnion extend to 1 year amnion, and preservation condition is a normal temperature, and therefore, transportation easily can meet clinical needs at any time.The present invention adopts freeze-drying, makes fresh amnion after freeze drying, long preservation at normal temperatures, and can keep the activity of its cytoactive and growth factor.Because store method is not subjected to the restriction of temperature, helps amnion dressing large-scale production, preservation,, alleviate clinical pressure for eye surface diseases patient and fire victim provide enough amnion dressing.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1:
Select for use health to cut open the placenta tissue that the puerpera is produced in the palace, antenatal Serological testing is carried out according to relevant national standard, gets rid of hepatitis B, third liver, syphilis and aids.Amnion and chorion blunt separation with placenta, clean, the amnion cuticular cellulose that obtains the glycerine submergence with 8% with the holder of nitrocellulose filter paper below last, adopt freeze drier, material is put 0 ℃~-30 ℃ pre-freezes in operating room 1~2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃~-45 ℃, vacuum cooling drying 3~8 hours, dried amnion can be preserved at normal temperatures through packing, sterilization.
Embodiment 2:
Select for use health to cut open the placenta tissue that the puerpera is produced in the palace, antenatal Serological testing is carried out according to relevant national standard, gets rid of hepatitis B, third liver, syphilis and aids.Amnion and chorion blunt separation with placenta, clean, the amnion cuticular cellulose that obtains the glycerine submergence with 10% with the holder of nitrocellulose filter paper below last, adopt freeze drier, material is put 0 ℃~-30 ℃ pre-freezes in operating room 1~2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃~-45 ℃, vacuum cooling drying 8 hours, dried amnion can be preserved at normal temperatures through packing, sterilization.
Embodiment 3:
Select for use health to cut open the placenta tissue that the puerpera is produced in the palace, antenatal Serological testing is carried out according to relevant national standard, gets rid of hepatitis B, third liver, syphilis and aids.Amnion and chorion blunt separation with placenta, clean, the amnion cuticular cellulose that obtains the glycerine submergence with 0.1% with the holder of nitrocellulose filter paper below last, adopt freeze drier, material is put 0 ℃~-30 ℃ pre-freezes in operating room 2 hours, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃~-45 ℃, vacuum cooling drying 5 hours, dried amnion can be preserved at normal temperatures through packing, sterilization.