CN1241474C - Amnia preservation and handling method - Google Patents
Amnia preservation and handling method Download PDFInfo
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- CN1241474C CN1241474C CNB031508383A CN03150838A CN1241474C CN 1241474 C CN1241474 C CN 1241474C CN B031508383 A CNB031508383 A CN B031508383A CN 03150838 A CN03150838 A CN 03150838A CN 1241474 C CN1241474 C CN 1241474C
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- amnions
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- amnion
- freeze drying
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Abstract
The present invention relates to a method for storing and processing biologic amnions. In the method, placental amnions are peeled from chorions in a passive mode and are washed; the epidermal cell layers of the obtained amnions are upward, and the lower surfaces of the amnions are supported by cellulose nitrate filter paper; the amnions are protected by 0.1% to 10% of glycerin by using a freeze drying technique; the materials are put in a working chamber and are froze in a beforehand method for 1 hours to 2 hours at 0 to 30 DEG C; a condensation chamber is opened for refrigeration; when cold trap temperature reaches subzero 35 DEG C to subzero 45 DEG C, a vacuum pump is opened for carrying out vacuum freeze drying for 3 hours to 8 hours; the materials are sealed, sterilized, dried and are stored in shade positions. The present invention causes the retention time of amnions to be lengthened to one year, causes amnions to be stored for a long time at normal temperature, to be conveniently transported and to satisfy clinic requirements at any time, can keep the cytoactive and the activity of growth factors of amnions, is favorable to the large-scale production and the storage of amnion dressing and provides sufficient amnion dressing for patients of eye surface diseases and burn patients for relieving clinical pressure.
Description
Technical field
The present invention relates to a kind of preservation treatment method of medicine equipment dressing amnion raw material.
Background technology
Amnion is because self exclusive characteristic receives the Chinese scholars concern, amnion is meant human placenta's basement membrane layer, it is the collagen tissue layer of no blood vessel, no cell structure, be rich in IV, collagen type v, basic fibroblast growth factor (BFGF), stem cell factor (HGF), the differentiation, the propagation that epithelial cell are divided a word with a hyphen at the end of a line grow into, promote epithelium play an important role in the epithelium wound healing.Amnion contains various albumen inhibiting factors, brings into play antiinflammatory action by suppressing the corresponding protein enzyme.Also contain simultaneously and suppress cytokine-expressing and regulate Apoptosis composition TGF-β
1, can avoid the collagenous fibres hyperplasia, the prevention adhesion.Reach a tissue repair and a purpose that table is rebuild and prevented to recur by above-mentioned mechanism.As a kind of new biomaterial, be widely used in the supplemental treatment of burn wound and ophthalmology disease in recent years both at home and abroad clinically, its curative effect highly significant.From domestic and foreign literature data report, used clinically amnion is fresh amnion, or the amnion that adopts profound hypothermia (80 ℃) to preserve.Fresh amnion often can not guarantee urgent clinical needs, and the amnion holding time that low temperature is preserved is short, maximum 1～2 month of its holding time, and transportation is inconvenient.
Summary of the invention
The objective of the invention is to: at the problems referred to above, provide that a kind of processing method is easy, long shelf-life, the bioamnion preservation treatment method that can meet clinical needs at any time.
Bioamnion preservation treatment method of the present invention is: with the amnion and the chorion blunt separation of placenta; clean; the amnion cuticular cellulose that obtains with the holder of nitrocellulose filter paper below last, the glycerine protection with 0.1～10%; adopt freeze drying process; material is put 0 ℃～-30 ℃ pre-freezes in operating room 1～2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃～-45 ℃; vacuum cooling drying 3～8 hours, sealing, sterilization, dry shady and cool place preserve.
Bioamnion preservation treatment method of the present invention through after the freeze drying, makes the holding time of amnion extend to 1 year amnion, and preservation condition is a normal temperature, and therefore, transportation easily can meet clinical needs at any time.The present invention adopts freeze-drying, makes fresh amnion after freeze drying, long preservation at normal temperatures, and can keep the activity of its cytoactive and growth factor.Because store method is not subjected to the restriction of temperature, helps amnion dressing large-scale production, preservation,, alleviate clinical pressure for eye surface diseases patient and fire victim provide enough amnion dressing.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
Embodiment 1:
Select for use health to cut open the placenta tissue that the puerpera is produced in the palace, antenatal Serological testing is carried out according to relevant national standard, gets rid of hepatitis B, third liver, syphilis and aids.Amnion and chorion blunt separation with placenta, clean, the amnion cuticular cellulose that obtains the glycerine submergence with 8% with the holder of nitrocellulose filter paper below last, adopt freeze drier, material is put 0 ℃～-30 ℃ pre-freezes in operating room 1～2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃～-45 ℃, vacuum cooling drying 3～8 hours, dried amnion can be preserved at normal temperatures through packing, sterilization.
Embodiment 2:
Select for use health to cut open the placenta tissue that the puerpera is produced in the palace, antenatal Serological testing is carried out according to relevant national standard, gets rid of hepatitis B, third liver, syphilis and aids.Amnion and chorion blunt separation with placenta, clean, the amnion cuticular cellulose that obtains the glycerine submergence with 10% with the holder of nitrocellulose filter paper below last, adopt freeze drier, material is put 0 ℃～-30 ℃ pre-freezes in operating room 1～2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃～-45 ℃, vacuum cooling drying 8 hours, dried amnion can be preserved at normal temperatures through packing, sterilization.
Embodiment 3:
Select for use health to cut open the placenta tissue that the puerpera is produced in the palace, antenatal Serological testing is carried out according to relevant national standard, gets rid of hepatitis B, third liver, syphilis and aids.Amnion and chorion blunt separation with placenta, clean, the amnion cuticular cellulose that obtains the glycerine submergence with 0.1% with the holder of nitrocellulose filter paper below last, adopt freeze drier, material is put 0 ℃～-30 ℃ pre-freezes in operating room 2 hours, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃～-45 ℃, vacuum cooling drying 5 hours, dried amnion can be preserved at normal temperatures through packing, sterilization.
Claims (1)
1, bioamnion preservation treatment method, it is characterized in that: with the amnion and the chorion blunt separation of placenta, clean, the amnion cuticular cellulose that obtains is last, glycerine submergence with 0.1-10%, adopt freeze drying process, material was put 0 ℃～-30 ℃ pre-freezes in operating room 1～2 hour, open the condensation chamber refrigeration, open vavuum pump when making condenser temperature reach-35 ℃～-45 ℃, vacuum cooling drying 3～8 hours is packed, is sterilized, and gets final product.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031508383A CN1241474C (en) | 2003-09-03 | 2003-09-03 | Amnia preservation and handling method |
| PCT/CN2004/001010 WO2005021014A1 (en) | 2003-09-03 | 2004-09-02 | Preparation of biological amnion preservable at room temperature |
| CNB2004800239337A CN100496238C (en) | 2003-09-03 | 2004-09-02 | Preparation method of bioamnion capable of preserving at normal temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031508383A CN1241474C (en) | 2003-09-03 | 2003-09-03 | Amnia preservation and handling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1522568A CN1522568A (en) | 2004-08-25 |
| CN1241474C true CN1241474C (en) | 2006-02-15 |
Family
ID=34286784
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031508383A Expired - Lifetime CN1241474C (en) | 2003-09-03 | 2003-09-03 | Amnia preservation and handling method |
| CNB2004800239337A Active CN100496238C (en) | 2003-09-03 | 2004-09-02 | Preparation method of bioamnion capable of preserving at normal temperature |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004800239337A Active CN100496238C (en) | 2003-09-03 | 2004-09-02 | Preparation method of bioamnion capable of preserving at normal temperature |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN1241474C (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1311072C (en) * | 2005-07-12 | 2007-04-18 | 何伟 | Preparation method of dried active amnion |
| ES2437865T3 (en) * | 2005-07-25 | 2014-01-14 | Foundation For Biomedical Research And Innovation | Composition in sheet form |
| CN101889569B (en) * | 2010-07-23 | 2013-05-22 | 马吉献 | Method for drying and storing biological amnion |
| CA3133869A1 (en) | 2011-08-26 | 2013-03-07 | Tissuetech, Inc. | Methods of sterilizing fetal support tissues |
| CN102631707A (en) * | 2012-05-04 | 2012-08-15 | 厦门大学 | Amnion-based biological material and preparation method and uses thereof |
| TW201603818A (en) | 2014-06-03 | 2016-02-01 | 組織科技股份有限公司 | Compositions and methods |
| WO2016138025A2 (en) | 2015-02-23 | 2016-09-01 | Tissuetech, Inc. | Apparatuses and methods for treating ophthalmic diseases and disorders |
| CN104826166B (en) * | 2015-05-06 | 2017-06-06 | 广州优适清生物科技有限公司 | A kind of biomembrane for glaucoma treatment and preparation method thereof |
| CN104825493B (en) * | 2015-05-06 | 2020-02-21 | 广州瑞泰生物科技有限公司 | Biological amniotic membrane for ocular surface treatment and preparation method thereof |
| CN105028386A (en) * | 2015-06-05 | 2015-11-11 | 热孜万·买买提明 | Preservation method of active amniotic membrane |
| CN107296041B (en) * | 2017-07-02 | 2020-12-25 | 江西瑞济生物工程技术股份有限公司 | Fresh amnion preservation solution, fresh amnion preservation method and application |
| AU2018301395A1 (en) * | 2017-07-11 | 2020-02-06 | Universal Stabilization Technologies, Inc. | Method for Preserving Biological Materials |
| CN109288867A (en) * | 2018-10-26 | 2019-02-01 | 上海慧存医疗科技有限公司 | A kind of preparation method of compound amnion powder preparation |
-
2003
- 2003-09-03 CN CNB031508383A patent/CN1241474C/en not_active Expired - Lifetime
-
2004
- 2004-09-02 CN CNB2004800239337A patent/CN100496238C/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN1522568A (en) | 2004-08-25 |
| CN100496238C (en) | 2009-06-10 |
| CN1871019A (en) | 2006-11-29 |
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Owner name: JIANGXI RUIJI BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME OR ADDRESS: SUMITOMO BIOENGINEERING CO., LTD. JIANGXI ACADEMY OF SCIENCES |
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Address after: 330029 No. 108 Fang Fang Road, Nanchang, Jiangxi Patentee after: JIANGXI RUIJI BIO-ENGINEERING TECHNOLOGY Co.,Ltd. Address before: 330029 No. 108 Fang Fang Road, Nanchang, Jiangxi Patentee before: Sumitomo Bioengineering Co.,Ltd. Jiangxi Academy of Sciences |
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Address after: 330096 Nanchang province high tech Development Zone, Jiangxi Road, No. 498 Patentee after: JIANGXI RUIJI BIOTECHNOLOGY Co.,Ltd. Address before: 330029 No. 108 Fang Fang Road, Nanchang, Jiangxi Patentee before: JIANGXI RUIJI BIO-ENGINEERING TECHNOLOGY Co.,Ltd. |
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