CN1488642A - Paddy rice ethylene receptor protein, coded gene and use thereof - Google Patents
Paddy rice ethylene receptor protein, coded gene and use thereof Download PDFInfo
- Publication number
- CN1488642A CN1488642A CNA021311544A CN02131154A CN1488642A CN 1488642 A CN1488642 A CN 1488642A CN A021311544 A CNA021311544 A CN A021311544A CN 02131154 A CN02131154 A CN 02131154A CN 1488642 A CN1488642 A CN 1488642A
- Authority
- CN
- China
- Prior art keywords
- sequence
- leu
- gene
- ser
- val
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 38
- 235000007164 Oryza sativa Nutrition 0.000 title description 17
- 235000009566 rice Nutrition 0.000 title description 13
- 108091054761 ethylene receptor family Proteins 0.000 title description 10
- 240000007594 Oryza sativa Species 0.000 title description 5
- 101710200602 Pyruvate kinase 1, cytosolic Proteins 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 11
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 claims abstract 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 11
- 125000000539 amino acid group Chemical group 0.000 claims description 7
- 150000001413 amino acids Chemical group 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 230000002411 adverse Effects 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- GOJUJUVQIVIZAV-UHFFFAOYSA-N 2-amino-4,6-dichloropyrimidine-5-carbaldehyde Chemical group NC1=NC(Cl)=C(C=O)C(Cl)=N1 GOJUJUVQIVIZAV-UHFFFAOYSA-N 0.000 claims description 3
- 108091000080 Phosphotransferase Proteins 0.000 claims description 3
- 102000020233 phosphotransferase Human genes 0.000 claims description 3
- 230000008034 disappearance Effects 0.000 claims description 2
- 101710154303 Cyclic AMP receptor protein Proteins 0.000 claims 5
- 230000000694 effects Effects 0.000 abstract description 2
- 241001597008 Nomeidae Species 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 21
- 241000209094 Oryza Species 0.000 description 12
- 102220023257 rs387907546 Human genes 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 230000006353 environmental stress Effects 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 2
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000036579 abiotic stress Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 102220369446 c.1274G>A Human genes 0.000 description 2
- 102220369447 c.1352G>A Human genes 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102220023256 rs387907547 Human genes 0.000 description 2
- 102220023258 rs387907548 Human genes 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 2
- 229920002554 vinyl polymer Polymers 0.000 description 2
- DKJPOZOEBONHFS-ZLUOBGJFSA-N Ala-Ala-Asp Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC(O)=O DKJPOZOEBONHFS-ZLUOBGJFSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- RXGLHDWAZQECBI-SRVKXCTJSA-N Leu-Leu-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O RXGLHDWAZQECBI-SRVKXCTJSA-N 0.000 description 1
- WMIOEVKKYIMVKI-DCAQKATOSA-N Leu-Pro-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O WMIOEVKKYIMVKI-DCAQKATOSA-N 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000010624 Medicago sativa Nutrition 0.000 description 1
- BQVUABVGYYSDCJ-UHFFFAOYSA-N Nalpha-L-Leucyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)CC(C)C)C(O)=O)=CNC2=C1 BQVUABVGYYSDCJ-UHFFFAOYSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- ILGCZYGFYQLSDZ-KKUMJFAQSA-N Phe-Ser-His Chemical compound N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(O)=O ILGCZYGFYQLSDZ-KKUMJFAQSA-N 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- DWPXHLIBFQLKLK-CYDGBPFRSA-N Pro-Pro-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H]1NCCC1 DWPXHLIBFQLKLK-CYDGBPFRSA-N 0.000 description 1
- LNICFEXCAHIJOR-DCAQKATOSA-N Pro-Ser-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O LNICFEXCAHIJOR-DCAQKATOSA-N 0.000 description 1
- 238000010240 RT-PCR analysis Methods 0.000 description 1
- 108010025216 RVF peptide Proteins 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 101150006914 TRP1 gene Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- GQHAIUPYZPTADF-FDARSICLSA-N Trp-Ile-Arg Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)=CNC2=C1 GQHAIUPYZPTADF-FDARSICLSA-N 0.000 description 1
- LVTKHGUGBGNBPL-UHFFFAOYSA-N Trp-P-1 Chemical compound N1C2=CC=CC=C2C2=C1C(C)=C(N)N=C2C LVTKHGUGBGNBPL-UHFFFAOYSA-N 0.000 description 1
- BCBFMJYTNKDALA-UFYCRDLUSA-N Val-Phe-Phe Chemical compound N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O BCBFMJYTNKDALA-UFYCRDLUSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 102220369445 c.668T>C Human genes 0.000 description 1
- 238000010523 cascade reaction Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000009746 freeze damage Effects 0.000 description 1
- 238000012214 genetic breeding Methods 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 108010051242 phenylalanylserine Proteins 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 108010010594 plant ethylene receptors Proteins 0.000 description 1
- 108010020432 prolyl-prolylisoleucine Proteins 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012882 sequential analysis Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of inverse correlation resistant ethane acceptor protein and the encrypt gene and the application. The protein's name is OsPK1, which has 2 cistine residue series in series table, or the derivant protein with the same activity to the former one. The encrypt gene is one of following nucleic acid series: 1)the DNA series in series table 1; 2) DAN series which has 90% similarity to the one limited in table 1 and the same encrypt function.
Description
Technical field
The present invention relates to plant ethylene receptor proteinoid and encoding gene thereof and application, particularly derive from ethylene receptor proteinoid and encoding gene and the application of paddy rice.
Background technology
Arid, saline and alkaline, damage to plants caused by sudden drop in temperature, abiotic stress such as freeze injury, waterlogging coerces factor and biotic factor such as disease and pest, growth and development of plant had material impact, can cause the extensive underproduction of farm crop when serious, cultivating the resistance of reverse crop is one of major objective of plant husbandry.Improve the resistance of reverse of crop, except utilizing traditional breeding method, at present, one of field that the molecular genetic breeding has become the scientific worker to be paid close attention to.Under the coercing of abiotic and biological adverse circumstance, higher plant cell has the number of ways impression and replys the variation of physico-chemical parameter in the external environment, the signal that born of the same parents are outer becomes intracellular signal, pass the signal along to nucleus through a series of phosphorylation cascade reactions, through the relevant functional gene of transcription factor regulation and control, start the expression of adverse circumstance response gene, improve the resistance of reverse of plant.Verified, one of signal pipeline of response abiotic and biological environment stress is relevant with ethene in the plant.
Paddy rice is illustrated the signal transmission of the ethene relational approach of its response abiotic and biological adverse circumstance, and then improves its resistance of reverse as one of most important food crop, has important theory and realistic meaning.
Summary of the invention
The purpose of this invention is to provide and anti-contrary relevant a kind of vinyl receptor protein and the encoding gene thereof of characteristic of plant.
Resistance of reverse vinyl receptor protein provided by the present invention derives from paddy rice, name is called OsPK1, be class protein, or the amino acid residue sequence of sequence 2 is passed through replacement, disappearance or the interpolation of one or several amino-acid residue and has identical active by sequence 2 deutero-protein with the amino acid residue sequence of sequence 2 with sequence 2 amino acid residue sequences in the sequence table.
Sequence 2 protein sequences are made up of 763 amino-acid residues in the sequence table, contain conservative GAF structural domain sequence, kinase domain sequence and susceptor structural domain sequence.GAF structural domain sequence be in the sequence 2 from the nitrogen end to carbon teminal 190-349 amino acids residue; The kinase domain sequence is to carbon teminal 375-615 amino acids residue from the nitrogen end; Susceptor structural domain sequence is to carbon teminal 640-756 amino acids residue from the nitrogen end.
The encoding gene of the ethylene receptor of anti-retrocorrelation proteinoid OsPK1 is one of following nucleotide sequences:
1) dna sequence dna of sequence 1 in the sequence table;
2) with sequence table in the dna sequence dna that limits of sequence 1 have 90% above homology, and the identical function protein DNA sequence of encoding.
The dna sequence dna of sequence 1 is by 2292 based compositions in the sequence table, and the reading frame of this gene is the full length DNA sequence, and it is expressed and mainly to be hurt and the inducing of high salt.
Utilize any carrier that can guide foreign gene in plant, to express, with the gene transfered plant cell of coding ethylene receptor proteinoid provided by the present invention, can obtain transgenic cell line and transfer-gen plant that biological environment stress tolerances such as arid and abiotic stress such as high salt and disease and pest are enhanced.Gene of the present invention can add any enhancing promotor or inducible promoter in being building up to plant expression vector the time before its transcription initiation Nucleotide.For the ease of transgenic plant cells or plant being identified and screening, can process employed carrier, as the antibiotic marker thing (gentamicin, kantlex etc.) that adds the alternative mark (gus gene, luciferase genes etc.) of plant or have resistance.Carry that OsPK1 expression carrier of the present invention can Ti-plasmids, Ri plasmid, plant viral vector, directly DNA conversion, microinjection, electricity be led, conventional biological method transformed plant cells or tissue such as agriculture bacillus mediated by using, and the plant transformed tissue cultivating is become plant.By the plant transformed host both can be monocotyledons, also can be dicotyledons, as: paddy rice, wheat, corn, cucumber, tomato, willow, turfgrass, lucerne place etc.Gene pairs of the present invention is cultivated the adversity resistant plant kind, particularly cultivates anti-abiotic and biological coercing as salt tolerant, disease and insect resistance plant variety, and it is significant to improve crop yield.
The present invention will be further described below in conjunction with drawings and the specific embodiments.
Description of drawings
The similarity of Fig. 1, OsPK1 and other ethylene receptor proteinoid gene relatively.
The protein structure pattern of Fig. 2, OsPK1.
Fig. 3, OsPK1 transcribe and are hurt and high-salt stress inductive RT-PCR analytical results.
Embodiment
The screening of embodiment 1, the paddy rice ethylene receptor of anti-retrocorrelation proteinoid OsPK1 and the clone of cDNA thereof
Amino acid conserved sequence with the known tobacco ethylene receptor of anti-retrocorrelation proteinoid gene NTHK1 carries out the BLAST retrieval in the paddy gene storehouse, the reads fragment that obtains through splicing, obtains the ethylene receptor of anti-retrocorrelation proteinoid gene.
Paddy rice (Oryza sativa var.Lansheng) seed kind grows to two leaves and carries out during one heart stage taking a sample after environment stress is handled in culturing room in culture dish.Collect fresh blade 1g and grind in liquid nitrogen, be suspended from the 4mol/L sulphur hydracid guanidine, mixture adds dehydrated alcohol and precipitates total RNA with acid phenol, chloroform extracting in the supernatant, afterwards, water-soluble.RNA is synthesized cDNA with reversed transcriptive enzyme.Sequences Design primer according to above-mentioned spliced gene through pcr amplification, obtains OsPK1.
OsPK1 is carried out sequential analysis, identical with the sequence that obtains in the database, as shown in Figure 1, have and ethylene receptor proteinoid NTHK1, the conservative region that NTHK2 etc. are similar.As shown in Figure 2, the aminoacid sequence of the inferring protein structure that draws according to nucleotide sequence.
The relation of embodiment 2, rice Os PK1 activity and environment-stress
Paddy rice (Oryza sativa var.Lansheng) seed kind grows to two leaves and carries out the following various processing of coercing during one heart stage in culturing room in culture dish:
Injury is handled: the paddy rice seedling is cut into 1cm length, put into water and vibrate.
Salt is handled: the paddy rice seedling is moved in the 1%NaCl solution.
Drought is handled: the paddy rice seedling is moved in 20%PEG 6000 solution.
In various processing back 0,0.5,1,3,8 hour (injury is handled) respectively; 0,3,8,24,48 hours (salt processing); 0, (drought is handled) sampling in 2,4,24 hours.Collect fresh blade 1g and grind in liquid nitrogen, be suspended from the 4mol/L sulphur hydracid guanidine, mixture adds dehydrated alcohol and precipitates total RNA with acid phenol, chloroform extracting in the supernatant, water-soluble afterwards, obtains total RNA.With OsPK1 DNA partial sequence is that primer carries out the RT-PCR analysis.Partial results as shown in Figure 3, A handles for injury among the figure; B is that 1%NaCl handles, and analysis revealed, OsPK1 are hurt and high salt is induced, but are not subjected to drought-induced.
＜160〉2＜210〉1＜211〉2292＜212〉DNA＜213〉 ( Oryza sativa var.Lansheng )＜400〉1atgccaccga tcccatctct gtggatccgc gtcttcttct cctggctgct gctgtcgctg 60cccgccgcgg ctgccgccga tttcagccac tgcggcggct gcgacgacgg cgacggcggc 120ggcggcatat ggagcacgga caacatcctg caatgccaga gggtgagcga cttcctcatc 180gccatggcct acttctccat cccgctcgag ctgctctact tcgccacctg ctccgacctc 240ttcccgctca agtggatcgt cctgcagttc ggcgccttca tcgtgctctg cggcctcacc 300cacctcatca ccatgttcac ctacgagccg cactcgttcc acgtcgtgct cgcgctcacc 360gtcgccaagt tcctgaccgc cctggtgtcg ttcgccaccg cgatcacgct gctcacgctc 420ataccgcagc tgctcagggt caaggtcagg gagaacttcc tcaggatcaa ggcgcgggag 480ctggaccggg aggtggggat gatgaagagg caagaggaag cgagttggca cgtgcggatg 540ctcacgcacg agatccgcaa gtcgctcgat cgccacacga tcctgtacac caccatggtt 600gagctctcca agacgctcga gctgcagaac tgcgccgtct ggatgcctag cgagagcggg 660agcgagatga tcctgacaca tcagctgagg cagatggaga cggaggactc gaatagcctg 720tccattgcga tggacaaccc ggatgtactt gaaataaagg caacaaagga tgcaaaagtt 780ctcgcggcag actcagcgct tgggattgca agcagaggca agcttgaagc aggtcctgtt 840gctgcaatac ggatgccgat gttgaaggcg tcgaatttca aaggagggac tccagaagtg 900atggaaacaa gctatgctat tttggtcttg gtactacccg aggatggttc actaggatgg 960ggtgaagagg agttggagat tgttgaagtg gttgctgatc aagtcgcagt cgccctatca 1020catgctgcag ttctggagga atctcaattg atgcgagaga agctcgccgc gcaacacagg 1080gacttgctga gagcaaagca tgaaaccacg atggccactg aagctaggaa ctcctttcag 1140actgccatgt atgatggaat gcgacggcca atgcactcga tccttggtct agtctcaatg 1200atgcagcaag aaaatatgaa ccctgagcaa aggcttgtaa tggatgccat tgtcaagaca 1260agtagcgttg cgtcaacatt gatgaatgat gtcatgcaaa catcaaccgt aaatcgtgag 1320tatttgtctt tggtgaggag ggcattcaac cttcattcgt tggttaagga ggcaatcagt 1380gttgtaagat gcctaactgg ctgcaagggg attgattttg agtttgaagt ggataattct 1440ttgcccgaaa gggtcgtcgg tgatgagaag agggttttcc acatcgtcct gcacatggta 1500ggtactctaa tacagaggtg taatgcaggc tgtctatcct tgtatgcgaa tacttacaat 1560gagaaggaag agaggcacaa tcaggattgg atgctgcgaa gagcaaactt ctctggcagc 1620tatgtgtgtg ttaagtttga gattaggatt agagagtcca gaggtaatct tttgagttcg 1680tcatccagtc ggagacttca agggcccaac tctaccagtt ctgagatggg gcttagcttc 1740aacatgtgca agaaaattgt gcagatgatg aatggtaata tttggtcagt ctctgattca 1800aaaggccttg gagaaactat catgcttgcc cttcagttcc agctgcagca tgtgactccg 1860gtctctggag catcctcgga cttgttccga tcagcgccaa ttcccaattt taatggactc 1920caagtcattc ttgtggacag tgatgacacc aatcgggccg taactcacaa gctcctggag 1980aagcttggtt gcctagtcct ctcggttact tccggcatcc aatgcatcaa ctcctttgcc 2040agtgctgagt catctttcca gctggtggtt cttgacctca ccatgcgtac aatggatgga 2100tttgatgtag ctcttgcaat caggaagttc agggggaatt gttggccacc gttgatagtt 2160gctcttgcgg caagcaccga tgacaccgtt cgggatcggt gccagcaggc aggaataaac 2220ggtctgatcc aaaaaccagt cactttagca gcactgggag atgaactgta tagagtcctt 2280caaaacaatt ga 2292＜210〉2＜211〉763＜212〉PRT＜213〉 ( Oryza sativa var.Lansheng )＜400〉2Met Pro Pro Ile Pro Ser Leu Trp Ile Arg Val Phe Phe Ser Trp 1 5 10 15Leu Leu Leu Ser Leu Pro Ala Ala Ala Ala Ala Asp Phe Ser His
20 25 30Cys?Gly?Gly?Cys?Asp?Asp?Gly?Asp?Gly?Gly?Gly?Gly?Ile?Trp?Ser
35 40 45Thr?Asp?Asn?Ile?Leu?Gln?Cys?Gln?Arg?Val?Ser?Asp?Phe?Leu?Ile
50 55 60Ala?Met?Ala?Tyr?Phe?Ser?Ile?Pro?Leu?Glu?Leu?Leu?Tyr?Phe?Ala
65 70 75Thr?Cys?Ser?Asp?Leu?Phe?Pro?Leu?Lys?Trp?Ile?Val?Leu?Gln?Phe
80 85 90Gly?Ala?Phe?Ile?Val?Leu?Cys?Gly?Leu?Thr?His?Leu?Ile?Thr?Met
95 100 105Phe?Thr?Tyr?Glu?Pro?His?Ser?Phe?His?Val?Val?Leu?Ala?Leu?Thr
110 115 120Val?Ala?Lys?Phe?Leu?Thr?Ala?Leu?Val?Ser?Phe?Ala?Thr?Ala?Ile
125 130 135Thr?Leu?Leu?Thr?Leu?Ile?Pro?Gln?Leu?Leu?Arg?Val?Lys?Val?Arg
140 145 150Glu?Asn?Phe?Leu?Arg?Ile?Lys?Ala?Arg?Glu?Leu?Asp?Arg?Glu?Val
155 160 165Gly?Met?Met?Lys?Arg?Gln?Glu?Glu?Ala?Ser?Trp?His?Val?Arg?Met
170 175 180Leu?Thr?His?Glu?Ile?Arg?Lys?Ser?Leu?Asp?Arg?His?Thr?Ile?Leu
185 190 195Tyr?Thr?Thr?Met?Val?Glu?Leu?Ser?Lys?Thr?Leu?Glu?Leu?Gln?Asn
200 205 210Cys?Ala?Val?Trp?Met?Pro?Ser?Glu?Ser?Gly?Ser?Glu?Met?Ile?Leu
215 220 225Thr?His?Gln?Leu?Arg?Gln?Met?Glu?Thr?Glu?Asp?Ser?Asn?Ser?Leu
230 235 240Ser?Ile?Ala?Met?Asp?Asn?Pro?Asp?Val?Leu?Glu?Ile?Lys?Ala?Thr
245 250 255Lys?Asp?Ala?Lys?Val?Leu?Ala?Ala?Asp?Ser?Ala?Leu?Gly?Ile?Ala
260 265 270Ser?Arg?Gly?Lys?Leu?Glu?Ala?Gly?Pro?Val?Ala?Ala?Ile?Arg?Met
275 280 285Pro?Met?Leu?Lys?Ala?Ser?Asn?Phe?Lys?Gly?Gly?Thr?Pro?Glu?Val
290 295 300Met?Glu?Thr?Ser?Tyr?Ala?Ile?Leu?Val?Leu?Val?Leu?Pro?Glu?Asp
305 310 315Gly?Ser?Leu?Gly?Trp?Gly?Glu?Glu?Glu?Leu?Glu?Ile?Val?Glu?Val
320 325 330Val?Ala?Asp?Gln?Val?Ala?Val?Ala?Leu?Ser?His?Ala?Ala?Val?Leu
335 340 345Glu?Glu?Ser?Gln?Leu?Met?Arg?Glu?Lys?Leu?Ala?Ala?Gln?His?Arg
350 355 360Asp?Leu?Leu?Arg?Ala?Lys?His?Glu?Thr?Thr?Met?Ala?Thr?Glu?Ala
365 370 375Arg?Asn?Ser?Phe?Gln?Thr?Ala?Met?Tyr?Asp?Gly?Met?Arg?Arg?Pro
380 385 390Met?His?Ser?Ile?Leu?Gly?Leu?Val?Ser?Met?Met?Gln?Gln?Glu?Asn
395 400 405Met?Asn?Pro?Glu?Gln?Arg?Leu?Val?Met?Asp?Ala?Ile?Val?Lys?Thr
410 415 420Ser?Ser?Val?Ala?Ser?Thr?Leu?Met?Asn?Asp?Val?Met?Gln?Thr?Ser
425 430 435Thr?Val?Asn?Arg?Glu?Tyr?Leu?Ser?Leu?Val?Arg?Arg?Ala?Phe?Asn
440 445 450Leu?His?Ser?Leu?Val?Lys?Glu?Ala?Ile?Ser?Val?Val?Arg?Cys?Leu
455 460 465Thr?Gly?Cys?Lys?Gly?Ile?Asp?Phe?Glu?Phe?Glu?Val?Asp?Asn?Ser
470 475 480Leu?Pro?Glu?Arg?Val?Val?Gly?Asp?Glu?Lys?Arg?Val?Phe?His?Ile
485 490 495Val?Leu?His?Met?Val?Gly?Thr?Leu?Ile?Gln?Arg?Cys?Asn?Ala?Gly
500 505 510Cys?Leu?Ser?Leu?Tyr?Val?Asn?Thr?Tyr?Asn?Glu?Lys?Glu?Glu?Arg
515 520 525His?Asn?Gln?Asp?Trp?Met?Leu?Arg?Arg?Ala?Asn?Phe?Ser?Gly?Ser
530 535 540Tyr?Val?Cys?Val?Lys?Phe?Glu?Ile?Arg?Ile?Arg?Glu?Ser?Arg?Gly
545 550 555Asn?Leu?Leu?Ser?Ser?Ser?Ser?Ser?Arg?Arg?Leu?Gln?Gly?Pro?Asn
560 565 570Ser?Thr?Ser?Ser?Glu?Met?Gly?Leu?Ser?Phe?Asn?Met?Cys?Lys?Lys
575 580 585Ile?Val?Gln?Met?Met?Asn?Gly?Asn?Ile?Trp?Ser?Val?Ser?Asp?Ser
590 595 600Lys?Gly?Leu?Gly?Glu?Thr?Ile?Met?Leu?Ala?Leu?Gln?Phe?Gln?Leu
605 610 615Gln?His?Val?Thr?Pro?Val?Ser?Gly?Ala?Ser?Ser?Asp?Leu?Phe?Arg
620 625 630Ser?Ala?Pro?Ile?Pro?Tyr?Phe?Asn?Gly?Leu?Gln?Val?Ile?Leu?Val
635 640 645Asp?Ser?Asp?Asp?Thr?Asn?Arg?Ala?Val?Thr?His?Lys?Leu?Leu?Glu
650 655 660Lys?Leu?Gly?Cys?Leu?Val?Leu?Ser?Val?Thr?Ser?Gly?Ile?Gln?Cys
665 670 675Ile?Asn?Ser?Phe?Ala?Ser?Ala?Glu?Ser?Ser?Phe?Gln?Leu?Val?Val
680 685 690Leu?Asp?Leu?Thr?Met?Arg?Thr?Met?Asp?Gly?Phe?Asp?Val?Ala?Leu
695 700 705Ala?Ile?Arg?Glu?Phe?Arg?Gly?Asn?Cys?Trp?Pro?Pro?Leu?Ile?Val
710 715 720Ala?Leu?Ala?Ala?Ser?Thr?Asp?Asp?Thr?Val?Arg?Asp?Arg?Cys?Gln
725 730 735Gln?Ala?Gly?Ile?Asn?Gly?Leu?Ile?Gln?Lys?Pro?Val?Thr?Leu?Ala
740 745 750Ala?Leu?Gly?Asp?Glu?Leu?Tyr?Arg?Val?Leu?Gln?Asn?Asn
755 760 763
Claims (9)
1, the ethane cyclic amp receptor protein of anti-retrocorrelation matter OsPK1, be protein, or the amino acid residue sequence of sequence 2 is passed through replacement, disappearance or the interpolation of one or several amino-acid residue and has identical active by sequence 2 deutero-protein with the amino acid residue sequence of sequence 2 with sequence 2 amino acid residue sequences in the sequence table.
2, ethane cyclic amp receptor protein matter according to claim 1 is characterized in that: it is the protein with sequence 2 amino acid residue sequences in the sequence table.
3, ethane cyclic amp receptor protein matter according to claim 2 is characterized in that: be the conservative territory of kinase function from the nitrogen end to carbon teminal 375-615 amino acids residue in the described sequence 2.
4, the encoding gene of the ethane cyclic amp receptor protein of anti-retrocorrelation matter OsPK1 is one of following nucleotide sequences:
1) dna sequence dna of sequence 1 in the sequence table;
2) with sequence table in the dna sequence dna that limits of sequence 1 have 90% above homology, and the identical function protein DNA sequence of encoding.
5, gene according to claim 4 is characterized in that: the encoding gene of described ethane cyclic amp receptor protein matter OsPK1 is the dna sequence dna of sequence 1 in the sequence table.
6, gene according to claim 5 is characterized in that: the reading frame of this gene is for holding the 1st to the 2292nd bit base from 3 '.
7, contain the described expression carrier of claim 4.
8, the clone that contains the described gene of claim 4.
9, the application of the described gene of claim 4 in cultivating the plant with adverse resistance kind.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02131154 CN1218959C (en) | 2002-10-11 | 2002-10-11 | Paddy rice ethylene receptor protein, coded gene and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02131154 CN1218959C (en) | 2002-10-11 | 2002-10-11 | Paddy rice ethylene receptor protein, coded gene and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1488642A true CN1488642A (en) | 2004-04-14 |
| CN1218959C CN1218959C (en) | 2005-09-14 |
Family
ID=34144801
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02131154 Expired - Fee Related CN1218959C (en) | 2002-10-11 | 2002-10-11 | Paddy rice ethylene receptor protein, coded gene and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1218959C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225375B (en) * | 2008-01-22 | 2010-10-13 | 北京未名凯拓作物设计中心有限公司 | Salt-endurance related rice protein kinase gene clone and uses thereof |
| CN106701785A (en) * | 2017-03-02 | 2017-05-24 | 广西壮族自治区农业科学院农产品加工研究所 | Mango ethylene receptor gene |
| CN110669118A (en) * | 2019-10-31 | 2020-01-10 | 河南农业大学 | Agaricus bisporus ethylene protein receptor |
| CN110669117A (en) * | 2019-10-31 | 2020-01-10 | 河南农业大学 | Volvariella volvacea ethylene receptor protein |
| CN111549041A (en) * | 2020-04-22 | 2020-08-18 | 青岛农业大学 | Ethylene-induced BAHD acyltransferase ERAT2 gene and application thereof |
-
2002
- 2002-10-11 CN CN 02131154 patent/CN1218959C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101225375B (en) * | 2008-01-22 | 2010-10-13 | 北京未名凯拓作物设计中心有限公司 | Salt-endurance related rice protein kinase gene clone and uses thereof |
| CN106701785A (en) * | 2017-03-02 | 2017-05-24 | 广西壮族自治区农业科学院农产品加工研究所 | Mango ethylene receptor gene |
| CN110669118A (en) * | 2019-10-31 | 2020-01-10 | 河南农业大学 | Agaricus bisporus ethylene protein receptor |
| CN110669117A (en) * | 2019-10-31 | 2020-01-10 | 河南农业大学 | Volvariella volvacea ethylene receptor protein |
| CN110669117B (en) * | 2019-10-31 | 2022-06-14 | 河南农业大学 | Volvariella volvacea ethylene receptor protein |
| CN110669118B (en) * | 2019-10-31 | 2022-06-14 | 河南农业大学 | Agaricus bisporus ethylene receptor protein |
| CN111549041A (en) * | 2020-04-22 | 2020-08-18 | 青岛农业大学 | Ethylene-induced BAHD acyltransferase ERAT2 gene and application thereof |
| CN111549041B (en) * | 2020-04-22 | 2022-07-08 | 青岛农业大学 | Ethylene-induced BAHD acyltransferase ERAT2 gene and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1218959C (en) | 2005-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101775398B (en) | Stress tolerance genetic engineering application of NAC protein gene DlNAC of chrysanthemum | |
| CN1814620A (en) | Soybean WRKY transcription factor GmWRKY6 and its coding gene and use | |
| CN1243098C (en) | Paddy rice anti-reverse transcripfactor and its coding gene and application | |
| CN105367644A (en) | Plant stress tolerance associated transcription factor, encoding gene of plant stress tolerance associated transcription factor, and application of encoding gene | |
| CN100344761C (en) | Boea clarkeane drought-resistant and salt-tolerance related gene and its coding protein and use | |
| CN100349916C (en) | PHD transcription factor of soybean and its coding gene and usage | |
| CN1216906C (en) | Transcription factor capable of regulating and controlling soybean adverse resistance, its coding gene and application | |
| CN1464907A (en) | Novel rice gene regulating salt stress tolerance | |
| CN1216904C (en) | Soybean ethylene response protein transcription factor and its coded gene and application | |
| CN1324137C (en) | Salt resistant and drought resistant gene of Thellugielkla halophila and its coded protein and use | |
| CN1488642A (en) | Paddy rice ethylene receptor protein, coded gene and use thereof | |
| CN1286849C (en) | Soybean transcripting factor, its coding gene and use thereof | |
| CN1710076A (en) | Use of boea crassifolia BcBCP1 gene for breeding drought-salt-tolerant plants | |
| CN1296383C (en) | Plant DREB transcription factor and its coding gene and uses | |
| CN101250220A (en) | Vegetable stress-resistant related protein as well as coding gene and application thereof | |
| CN1216903C (en) | Ethylene response conjugated protein transcription factor of ice plant and its coded gene and application | |
| CN1472222A (en) | DREB transcription factor of corn and its encoding genes and use | |
| CN1216905C (en) | Transcription factor for regulating and controlling dry land cotton anti contrary property and its coded gene and application | |
| CN102250230A (en) | Rice OsI2 protein, gene for coding protein and application | |
| CN105175522B (en) | Crowtoe AP2/ERF transcription factors and its encoding gene and application | |
| CN1908011A (en) | Plant inverse-resistant zinc finger protein, coding gene and application thereof | |
| CN1821405A (en) | Xinjiang Saussurea involucrata cold regulation protein and its code gene and use | |
| CN108034662A (en) | The breeding method of application and Rust resistance bacterium wheat of the wheat stripe rust PSTG_06025 genes in stripe rust prevention | |
| CN1216907C (en) | Adverse-resistance transcription factor from tomato, its coding gene and application | |
| CN1548453A (en) | Frigostable correlative transcriptive factor of rice and its coding gene and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |