CN1483507A - Method for mfg of acetate rayon film used for fully antomatic electrophoresis apparatus - Google Patents
Method for mfg of acetate rayon film used for fully antomatic electrophoresis apparatus Download PDFInfo
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- CN1483507A CN1483507A CNA031322603A CN03132260A CN1483507A CN 1483507 A CN1483507 A CN 1483507A CN A031322603 A CNA031322603 A CN A031322603A CN 03132260 A CN03132260 A CN 03132260A CN 1483507 A CN1483507 A CN 1483507A
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Abstract
The production method of acetate fibre film for full-automatic electrophoretic apparatus includes the following steps: mixing two cellulose acetates, its solvent and non-solvent, stirring them and dissolving to obtain film-moulding liquor; filtering, standing still and defoaming; applying said film-moulding liquor on the plastic substrate for moulding film; gelling and solidifying said film-moulding liquor; and making after-treatment so as to obtain finished product film with good strength.
Description
Technical field
The present invention relates to a kind of preparation method of film, be specifically related to a kind of preparation method who is used for the cellulose acetate film of full-automatic electrophoresis instrument.
Background technology
Electrophoretic techniques is one of important laboratory facilities of medical science, life science, the serum protein electrophoresis analysis has important value in clinical diagnosis, cellulose acetate film commonly used at present, be single layer structure, thickness is only had an appointment 120 microns, very soft, experimental implementation is very inconvenient, particularly, enter stage of automatical analysis at electrophoresis analytical method, this cellulose acetate film can't be suitable for, and full-automatic analysis of electrophoresis has good analytical precision, and save time, advantage such as laborsaving, be the direction of current electrophoretic analysis.Be directed to this situation, China utility model patent CN2469453Y discloses a kind of electrophoresis experiment carrier film, it is incorporated into the acetate fiber film body on the plastics dura mater by conformable layer, by the plastics dura mater film body is played a supportive role, according to its instructions as seen, conformable layer in this scheme is actually bonding coat, be to be bonding between acetate fiber film body and the plastics dura mater, because the existence of bonding process, make product thickness be difficult to control, and bonding coat may influence experiment effect in experimentation, also easily comes off.
Summary of the invention
The object of the invention provides a kind of manufacture method of cellulose acetate film, and the cellulose acetate film of acquisition is combined with substrate well, can satisfy the request for utilization of full-automatic electrophoresis instrument.
For achieving the above object, the technical solution used in the present invention is: a kind of manufacture method that is used for the cellulose acetate film of full-automatic electrophoresis instrument may further comprise the steps:
(1) provide a kind of casting solution, described casting solution mixes stirring and dissolving by cellulose diacetate, its solvent and non-solvent and forms;
(2) above-mentioned casting solution is filtered, leaves standstill froth breaking;
(3) filtered casting solution is coated casting film on the plastic substrate;
(4), make on-chip casting solution gel solidification through solvent evaporation;
(5) promptly obtain finished film through aftertreatment.
In the technique scheme, common, but not and as limit, described solvent is an acetone, non-solvent comprises deionized water, alkaline solution and grease.
And in order to increase the density of product, described non-solvent can also include ethanol.
The effect content of each composition of casting solution preferably is that per 100 gram cellulose diacetates add
600 milliliters～800 milliliters in acetone
100 milliliters～150 milliliters of ethanol
250 milliliters～300 milliliters of deionized waters
50 milliliters～75 milliliters of 10% alkaline solutions
3 milliliters～4 milliliters of greases.
The mixing order of casting solution is, earlier cellulose diacetate is dissolved in the acetone, fully after the dissolving, adds ethanol, deionized water, 10% alkaline solution, grease successively.
In the technique scheme, described substrate is polyethylene terephthalate (PET) substrate.
The time of described step 4 is 10 minutes to 30 minutes.
Described aftertreatment is with solvent and non-solvent in the water retting replacement film, makes drying and dehydrating then and handles.
Because the technique scheme utilization, the present invention compared with prior art has following advantage:
1. because the present invention is produced on casting solution on the PET substrate with coating method, and the cellulose acetate film that make to obtain has intensity preferably under the support of substrate, be convenient to the carrying out of testing, be particularly suitable for the full-automatic electrophoresis instrument and use;
2. coating method makes the diacetate fiber rete behind the gel combine closely with the PET substrate, and situation can not come off;
3. the adding of ethanol makes the membrane product of acquisition that density preferably be arranged;
4. the employing of alkaline solution has strengthened the electric conductivity of film, helps the quick experiment in the full-automatic electrophoresis instrument.
Description of drawings
Accompanying drawing 1 is the process chart of the embodiment of the invention one;
Accompanying drawing 2 is that casting solution is formed the phasor that changes in the embodiment of the invention one solvent evaporation process;
Accompanying drawing 3 is the product structure cross-sectional schematic of embodiment one.
Wherein: [1], casting solution; [2], two-phase region; [3], monophase field; [4], substrate; [5], porous support layer; [6], tight zone.
Embodiment
Below in conjunction with drawings and Examples the present invention is further described:
Embodiment one: to shown in the accompanying drawing 3, a kind of manufacture method that is used for the cellulose acetate film of full-automatic electrophoresis instrument may further comprise the steps referring to accompanying drawing 1:
(1) with 100 gram cellulose diacetate (CA
2) be dissolved in 650 milliliters of acetone, be made into the solution of certain solid content, left standstill then 8 hours, it is fully dissolved evenly, add 300 milliliters of 100 milliliters of ethanol, deionized water, 10% 60 milliliters of alkaline solutions, 3 milliliters of greases successively, stir rapidly;
(2) above-mentioned casting solution being left standstill froth breaking filtered after 8 hours;
(3) filtered casting solution is coated casting film on the PET substrate;
(4) at room temperature, through 10～30 minutes evaporation, casting solution began phase change and turns white, and gel solidification takes place;
(5) put into water, the solvent exchange in the casting solution is come out, form moisture porous wet film, drying dehydration more promptly obtains finished film.
Referring to accompanying drawing 2, casting solution 1 is in evaporation process, and 3 through two-phase region 2 arrival B points from A point monophase field, form to have the anisotropic membrane goods of the porous support layer of tight zone as thin as a wafer.
The acetate fiber membrane structure that obtains with present embodiment on PET transparent substrate 4, is porous support layer 5 as shown in Figure 3, and the surface forms tight zone 6.
Test with the cellulose acetate film that present embodiment obtains, adopt gondola MCROTECH648PC electrophoresis apparatus, test case is as follows:
(1) analysis condition is set: by the instrument requirement, electrophoresis time 15 minutes, dyeing 3 minutes, decolouring each 1 minute (wash-out 3 times), transparent 3 minutes, dried voltage 179V, electric current＞5 milliampere 10 minutes;
(2) analytical approach: undertaken by the instrumental analysis requirement, all analytical procedures are automatically carries out;
(3) repeatability is analyzed: adopt same serum sample to be divided into 8 parts and test and calculate its precision (CV) on same diaphragm, result, 5 districts band (albumin, α of demonstration
1Globulin, α
2Globulin, beta Globulin, gamma Globulin) CV be respectively 1.6%, 0.36%, 1.02%, 0.81%, 1.51%, prove that this diaphragm has good test precision;
(4) betweenrun precision analysis: adopt continuous 20 test analysis on different diaphragms of same routine serum sample at random, the result shows, article 5, the CV of district's band is respectively 1.86%, 1.03%, 1.06%, 1.13%, 1.34%, demonstrates good betweenrun precision equally.
Test result shows, the cellulose acetate film that present embodiment obtains have in good test precision and good batch and batch between repeatability, be suitable for each hospital clinical trial chamber and promote the use of.
Claims (8)
1. a manufacture method that is used for the cellulose acetate film of full-automatic electrophoresis instrument is characterized in that, may further comprise the steps:
(1) provide a kind of casting solution, described casting solution mixes stirring and dissolving by cellulose diacetate, its solvent and non-solvent and forms;
(2) above-mentioned casting solution is filtered, leaves standstill froth breaking;
(3) filtered casting solution is coated casting film on the plastic substrate;
(4), make on-chip casting solution gel solidification through solvent evaporation;
(5) promptly obtain finished film through aftertreatment.
2. the manufacture method of cellulose acetate film according to claim 1, it is characterized in that: described solvent is an acetone, and non-solvent comprises deionized water, alkaline solution and grease.
3. the manufacture method of cellulose acetate film according to claim 2, it is characterized in that: described non-solvent also includes ethanol.
4. the manufacture method of cellulose acetate film according to claim 3 is characterized in that: the content of each composition of casting solution is that per 100 gram cellulose diacetates add
600 milliliters～800 milliliters in acetone
100 milliliters～150 milliliters of ethanol
250 milliliters～300 milliliters of deionized waters
50 milliliters～75 milliliters of 10% alkaline solutions
3 milliliters～4 milliliters of greases.
5. according to the manufacture method of claim 3 or 4 described cellulose acetate films, it is characterized in that: the mixing order of casting solution is, earlier cellulose diacetate is dissolved in the acetone, fully after the dissolving, adds ethanol, deionized water, 10% alkaline solution, grease successively.
6. the manufacture method of cellulose acetate film according to claim 1, it is characterized in that: described substrate is a polyethylene terephthalate substrate.
7. the manufacture method of cellulose acetate film according to claim 1, it is characterized in that: the time of described step 4 is 10 minutes to 30 minutes.
8. the manufacture method of cellulose acetate film according to claim 1 is characterized in that: described aftertreatment is made drying and dehydrating then and is handled for replace solvent and the non-solvent in the film with water retting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03132260 CN1235670C (en) | 2003-08-04 | 2003-08-04 | Method for mfg of acetate rayon film used for fully antomatic electrophoresis apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03132260 CN1235670C (en) | 2003-08-04 | 2003-08-04 | Method for mfg of acetate rayon film used for fully antomatic electrophoresis apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1483507A true CN1483507A (en) | 2004-03-24 |
| CN1235670C CN1235670C (en) | 2006-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03132260 Expired - Fee Related CN1235670C (en) | 2003-08-04 | 2003-08-04 | Method for mfg of acetate rayon film used for fully antomatic electrophoresis apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918467A (en) * | 2017-04-14 | 2017-07-04 | 长庆石油勘探局技术监测中心 | A kind of method for successively gathering iron-based material corrosion product |
-
2003
- 2003-08-04 CN CN 03132260 patent/CN1235670C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106918467A (en) * | 2017-04-14 | 2017-07-04 | 长庆石油勘探局技术监测中心 | A kind of method for successively gathering iron-based material corrosion product |
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| Publication number | Publication date |
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| CN1235670C (en) | 2006-01-11 |
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| SE01 | Entry into force of request for substantive examination | ||
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Granted publication date: 20060111 |