CN2603389Y - Glucose oxidase electrode - Google Patents
Glucose oxidase electrode Download PDFInfo
- Publication number
- CN2603389Y CN2603389Y CNU032111029U CN03211102U CN2603389Y CN 2603389 Y CN2603389 Y CN 2603389Y CN U032111029 U CNU032111029 U CN U032111029U CN 03211102 U CN03211102 U CN 03211102U CN 2603389 Y CN2603389 Y CN 2603389Y
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- Prior art keywords
- glucose oxidase
- electrode
- glutaraldehyde
- adsorbed layer
- adsorption layer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
Abstract
Disclosed is a new type glucose oxidase electrode, which relates to an electrode glucose sensor component, especially a glucose oxidase electrode. The utility model comprises a graphite mixture conductor (2), a polyaniline film layer (4), a cellulose film layer (5), a glutaraldehyde adsorption layer (6), a glucose oxidase adsorption layer (7) and a glutaraldehyde adsorption layer (8), the polyaniline film layer (4), the cellulose film layer (5), the glutaraldehyde adsorption layer (6), the glucose oxidase adsorption layer (7) and the glutaraldehyde adsorption layer (8) are bonded on the lower end of the graphite mixture conductor (2) in sequence. The utility model solves the problem that the prior glucose oxidase electrode has high cost, great production difficulty, poor noise immunity, slow response and poor stability.
Description
Technical field: the utility model relates to a kind of element of electrode type glucose sensor, particularly a kind of glucose oxidase electrode.
Background technology: glucose oxidase electrode is as the electrode type glucose sensor of molecular recognition elements, and it is come out the earliest biology sensor, the time of existing four more than ten years so far.Glucose oxidase electrode is that the enzyme electrode of representative is the focus of present field of biosensors research.The enzyme electrode sensor with advantages such as the selectivity of enzyme reaction, sensitivity and electrochemical reaction simple to operate, respond fast that advantage combines, formed a kind of new analyzing and testing means.The enzyme electrode sensor has a wide range of applications at aspects such as Electroanalytical Chemistry, clinical chemistry, biomedicine, food industry and environmental monitorings, obtained numerous researchers' concern, the research paper of delivering in recent years increases year by year, research work related to basic electrode material and structure, enzyme immobilization technology, suppress the theoretical analysis etc. of method, detection means and the response signal of interfering material response.The practicalization of comparing enzyme electrode with the quantity of research paper is quite slow, this be because (1) present most usefulness of enzyme electrode member all be noble metal platinum or gold, owing to cost an arm and a leg, scarcity of resources, be unsuitable for producing in enormous quantities, although many in recent years researchers attempt to replace noble metal with carbon electrode,, limited the progress of practical research because the performance of electrode and manufacturing process do not solve; (2) one of committed step of enzyme electrode making---the immobilization technology of enzyme is still waiting to improve, so that improve the storage stability of enzyme electrode, improves the consistance of response, and the requirement of suitable mass industrialized production; (3) the composition complexity of actual sample, and the enzyme electrode of laboratory development is proofreaied and correct with standard solution usually, can run in use from the interference that has competitive response material in the actual sample or the pollution of other compositions, cause the selectivity of enzyme electrode to reduce, shorten serviceable life.
The utility model content: the utility model provides a kind of novel glucose oxidase electrode, and it has solved cost height that existing glucose oxidase electrode exists, the production difficulty is big, anti-interference is poor, the problem of low-response, poor stability.The utility model comprises graphite mixture electric conductor 2, polyaniline rete 4, cellulose rete 5, glutaraldehyde adsorbed layer 6, glucose oxidase adsorbed layer 7, glutaraldehyde adsorbed layer 8, is bonded with polyaniline rete 4, cellulose rete 5, glutaraldehyde adsorbed layer 6, glucose oxidase adsorbed layer 7 and glutaraldehyde adsorbed layer 8 successively in the bottom of graphite mixture electric conductor 2.Glucose oxidase electrode of the present invention has that the preparation method is simple, cost is low, strong interference immunity, response are fast, the advantage of good stability, and it can be used for the measurement of blood sugar and fermentation liquor glucose.The stable response time of this electrode is 20-40 second; Sensitivity is 100nA/mmolL
-1Linear response range to glucose is 0.01-3.0mmolL
-1, the linearity test lower limit is low; Good stability stores 15 days, the response current no change of enzyme electrode; And after the enzyme electrode response performance descended, the basic electrode material can reuse through polishing; The preparation expense is low, and the expense of its plinth electrode only is 0.20 yuan/; The expense of immobilised enzymes is about 0.35 yuan/; Suppress the interfering material response, the oxidation current of ascorbic acid only is original 7.7% in the solution, and the oxidation current of urea acid does not detect.
Description of drawings: Fig. 1 is a structural representation of the present utility model, and Fig. 2 is the vertical view of Fig. 1.
Embodiment: the glucose oxidase electrode of present embodiment is made up of glass tube 1, graphite mixture electric conductor 2, contact conductor 3, polyaniline rete 4, cellulose rete 5, glutaraldehyde adsorbed layer 6, glucose oxidase adsorbed layer 7, glutaraldehyde adsorbed layer 8; Be bonded with polyaniline rete 4, cellulose rete 5, glutaraldehyde adsorbed layer 6, glucose oxidase adsorbed layer 7 and glutaraldehyde adsorbed layer 8 successively in the bottom of graphite mixture electric conductor 2.This glucose oxidase electrode is cylindrical.Fixedly connected with the bottom of glass tube 1 in the upper end of graphite mixture electric conductor 2, the contact conductor 3 of fixedlying connected with graphite mixture electric conductor 2 is arranged in the glass tube 1.
Claims (4)
1, a kind of novel glucose oxidase electrode, it is characterized in that it comprises graphite mixture electric conductor (2), polyaniline rete (4), cellulose rete (5), glutaraldehyde adsorbed layer (6), glucose oxidase adsorbed layer (7), glutaraldehyde adsorbed layer (8), is bonded with polyaniline rete (4), cellulose rete (5), glutaraldehyde adsorbed layer (6), glucose oxidase adsorbed layer (7) and glutaraldehyde adsorbed layer (8) successively in the bottom of graphite mixture electric conductor (2).
2, a kind of novel glucose oxidase electrode according to claim 1 is characterized in that this glucose oxidase electrode is cylindrical.
3, a kind of novel glucose oxidase electrode according to claim 1 is characterized in that the upper end of graphite mixture electric conductor (2) fixedlys connected with the bottom of glass tube (1).
4, a kind of novel glucose oxidase electrode according to claim 3 is characterized in that the contact conductor (3) of fixedlying connected with graphite mixture electric conductor (2) is arranged in the glass tube (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU032111029U CN2603389Y (en) | 2003-01-20 | 2003-01-20 | Glucose oxidase electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU032111029U CN2603389Y (en) | 2003-01-20 | 2003-01-20 | Glucose oxidase electrode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2603389Y true CN2603389Y (en) | 2004-02-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU032111029U Expired - Fee Related CN2603389Y (en) | 2003-01-20 | 2003-01-20 | Glucose oxidase electrode |
Country Status (1)
| Country | Link |
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| CN (1) | CN2603389Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101251506B (en) * | 2008-03-15 | 2011-06-15 | 西北师范大学 | Method for manufacturing conductive polyaniline/cellulose composite biosensor |
| CN102320687A (en) * | 2011-06-18 | 2012-01-18 | 山东大学 | The preparation method of a kind of polyaniline-mikrobe combined electrode |
| CN101216449B (en) * | 2008-01-10 | 2012-01-18 | 沈阳师范大学 | Preparation method of polyaniline modified electrode for recognizing dead-live cell |
| CN101530328B (en) * | 2009-04-20 | 2013-06-19 | 湖州美奇医疗器械有限公司 | Hypodermic glucose sensor and making method thereof |
| CN105102972A (en) * | 2013-01-11 | 2015-11-25 | 东北大学 | Saliva glucose monitoring system |
-
2003
- 2003-01-20 CN CNU032111029U patent/CN2603389Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101216449B (en) * | 2008-01-10 | 2012-01-18 | 沈阳师范大学 | Preparation method of polyaniline modified electrode for recognizing dead-live cell |
| CN101251506B (en) * | 2008-03-15 | 2011-06-15 | 西北师范大学 | Method for manufacturing conductive polyaniline/cellulose composite biosensor |
| CN101530328B (en) * | 2009-04-20 | 2013-06-19 | 湖州美奇医疗器械有限公司 | Hypodermic glucose sensor and making method thereof |
| CN102320687A (en) * | 2011-06-18 | 2012-01-18 | 山东大学 | The preparation method of a kind of polyaniline-mikrobe combined electrode |
| CN105102972A (en) * | 2013-01-11 | 2015-11-25 | 东北大学 | Saliva glucose monitoring system |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |