CN106757447B - A kind of silk fibroin protein nanofiber and preparation method thereof - Google Patents
A kind of silk fibroin protein nanofiber and preparation method thereof Download PDFInfo
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- CN106757447B CN106757447B CN201611099113.7A CN201611099113A CN106757447B CN 106757447 B CN106757447 B CN 106757447B CN 201611099113 A CN201611099113 A CN 201611099113A CN 106757447 B CN106757447 B CN 106757447B
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- silk fibroin
- fibroin protein
- nanofiber
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- fibroin
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/42—Formation of filaments, threads, or the like by cutting films into narrow ribbons or filaments or by fibrillation of films or filaments
- D01D5/423—Formation of filaments, threads, or the like by cutting films into narrow ribbons or filaments or by fibrillation of films or filaments by fibrillation of films or filaments
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D1/00—Treatment of filament-forming or like material
- D01D1/02—Preparation of spinning solutions
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
- D01F4/02—Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin
Abstract
The present invention discloses a kind of silk fibroin protein nanofiber and preparation method thereof.Using silkworm silk as raw material, by dissolution regeneration extraction, hydrophily silk fibroin protein peptide chain, then the self assembly in mild aqueous environment are obtained, obtaining diameter is 20~500 nm, and length is up to 1~100 μm of nanofiber.The present invention prepare silk fibroin protein nanofiber without be added any additive can self assembly obtain the nanofiber of longer dimension, the brief, mild condition with technique, the characteristics of low production cost, the nanofiber being prepared can meet actual operation requirements.
Description
Technical field
The present invention relates to a kind of nanofibers, and in particular to a kind of nanofiber prepared using silk fibroin protein as raw material
And preparation method thereof.
Background technique
Natural silk fibroin protein fiber gains great popularity because of its excellent tensile strength and ductility, is widely used in light
The fields such as industry, medical treatment.Silk fibroin protein is regenerated because of the excellent biocompatibility of its native protein substrate, lower immunogene
The advantages such as property, low irritability, mild water phase processing environment, people to its tissue repair, medicine controlled releasing, surface of a wound auxiliary material and
The research report of beauty treatment fields is commonplace.However, either systems such as regenerated silk fibroin film, porous support, gel, all
Hardly match the excellent physical mechanical property of natural fibroin fiber.The fibroin albumen system regenerated is all often on microcosmic
Isotropism system, it is difficult to meet the requirement that anisotropisation cell culture and regenerated silk fibroin substrate are reinforced.
The study found that naturally the excellent physical mechanical property of silk fibroin protein fiber comes not only from high proportion and along fibre
Tie up the II type crystalline texture of Silk of axial alignment, also from fibrous inside by the II type crystalline solid of Silk of orientations and
The orientations for the fibril that unformed state of aggregation strand is constituted.For this purpose, people post through regeneration induction fibroin albumen system
In fibrosis skeleton structure enhance the systems such as regenerated silk fibroin gel, porous support and film, or by three-dimensional each
The external oriented growth of fiber skeleton structure Induction of neuronal, myocyte, canthus theca cell that anisotropy arranges etc. is simultaneously finally used
In in vivo functionality tissue repair.
Currently, the preparation method of nanofiber mainly has Static Spinning, bubble chamber film the traditional handicrafts such as split, are meltblown and by big
The method of molecule.Chinese invention patent " a kind of silk fibroin nano-fiber film and preparation method thereof " (103341209A) and " one
Kind prepare the device of functional nano fiber " (105734697A) etc. be the method preparation that Static Spinning is split by routine or film
Nanometer medium-length fibre, needs to consume the higher energy and dedicated large scale equipment, and production cost is larger, and the Nanowire prepared
Dimension is deposited on two-dimensional surface, it is difficult to meet the three-dimensional requirement used；Chinese invention patent be " a kind of linear nanometre hydroxylapatite
Preparation method " (102491298B) and " a kind of amorphous silk fibroin nano-fiber solution and preparation method thereof " (
Nanofiber 105968384A) etc. is prepared by the method for induction culturing self assembly, though without fixed large type equipment, system
Standby obtained nanofiber length is short, micron level is not achieved, it is difficult to meet actual operation requirements.
Summary of the invention
It is brief, mild condition, low production cost from group that in view of the deficiencies of the prior art, the present invention provides a kind of techniques
Fill silk fibroin protein nanofiber and preparation method thereof.
Realize that the technical solution of the object of the invention is to provide a kind of preparation method of silk fibroin protein nanofiber, with family
Silkworm silk is raw material, first carries out degumming, is dried, obtains pure fibroin fiber, then carry out following steps:
(1) pure silk fibroin Fibrinolysis is obtained into bombyx mori silk fibroin in the lithium bromide dissolution that concentration is 9.3M after dialysis
Protein solution is cast in mold, and under conditions of temperature is 20~25 DEG C, humidity is 45~65%, drying and moulding is thickness
0.5~5 millimeter of fibroin protein film；
(2) under room temperature, fibroin protein film is placed in ethyl alcohol and is impregnated 5~120 minutes, obtain the fibroin egg of recrystallization
Tunica albuginea；
(3) 1/100~1/10 w/v in proportion, it is 25~50 DEG C that the fibroin protein film of recrystallization, which is soaked in temperature,
In buffer, soaking time is 0.5~2 h；Again through 3000~10000 revs/min of centrifugal treatings, separation obtains supernatant；
(4) supernatant of acquisition is diluted to 0.1~10mg/ml, is cultivated in water bath with thermostatic control environment, obtains hygrometric state
Silk fibroin nano-fiber aggregation；
(5) hygrometric state silk fibroin nano-fiber aggregation is subjected to freeze-drying process, obtains silk fibroin protein nanometer
Fiber.
In technical solution of the present invention, the buffer is the trishydroxymethylaminomethane-hydrochloric acid or phosphate of pH=7.0
Buffer, buffer concentration are 0.01~1 M.
The water bath with thermostatic control environment, temperature are 25~50 DEG C；Incubation time is 3~8 h.
Technical solution of the present invention further includes a kind of silk fibroin protein nanofiber being prepared according to the above method, straight
Diameter is 20~500 nm, and length is 1~100 μm.
The principle of the present invention is: since hydrophily silk fibroin protein is in aqueous environment, having and compares conventional regeneration man
The conformation that fibroin protein more stretches, the electrostatic repulsion between the characteristic for being easy to mutually assemble and colloid micelle more tend to it
The characteristics of linear aggregation, therefore there is hydrophilic fibroin albumen colloidal dispersion biggish fibrosis under temperate condition to assemble power, from
And β-sheetization self aggregation is the nanofiber with long length quickly.In the technical solution of the present invention, tune can be passed through
The dipping pretreatment time of fibroin protein film in ethanol is saved, the diameter and length of nanofiber are controlled；Soaking time is longer, parent
Hydrophilic amino acid ratio is bigger in aqueous extract, and the time under isoconcentration is shorter, and the thinner institute the shorter at fiber；It is on the contrary then get over
It is thick longer.
Compared with prior art, present invention has an advantage that
1, compared with existing Static Spinning, meltblown and bubble chamber film split the techniques such as method, hydrophilic-silk of the present invention
It is 20~500 nm that fibroin solution can generate diameter in mild aqueous environment, and length is 1~100 μm of nanofiber, energy
Consume low, technique it is brief, without specific large type equipment, greatly reduce production cost.
2, compared with existing inductivity self-assembling technique, the present invention prepares silk fibroin protein nanofiber and appoints without being added
What additive can self assembly obtain the nanofiber of longer dimension, length increases by hundred times or more, can satisfy actual use
It is required that.
Detailed description of the invention
Fig. 1 is the electromicroscopic photograph figure after silk fibroin nano-fiber freeze-drying provided in an embodiment of the present invention.
Specific embodiment
In the following with reference to the drawings and specific embodiments, technical solution of the present invention is further elaborated.
Embodiment 1
The present embodiment provides a kind of preparation methods of silk fibroin nano-fiber, the specific steps are as follows:
(1) after the lithium bromide dissolution, dialysis of the pure fibroin fiber 9.3M by degumming after dry, fresh silkworm is obtained
Silk fibroin protein solution is cast in polystyrene mould, and sample path length is 1 millimeter, the fast rapid-curing cutback under 25 DEG C, 50% humidity environment
Dry molding.
(2) rapid draing fibroin protein film after molding is impregnated 30 minutes in ethyl alcohol at room temperature, obtains recrystallization silk
Fibroin film.
(3) will recrystallization treated fibroin protein film in 1/30(w/v) ratio is soaked in 37 DEG C, 0.001M, pH=
In 7.0 tris- hydrochloride buffer, soaking time 0.5h, then with 5000 revs/min of 14 min of centrifugal treating, separation is obtained
Obtain supernatant.
(4) after supernatant achieved above being diluted to 0.5mg/ml, 3h is cultivated in water bath with thermostatic control environment, obtains hygrometric state
Silk fibroin nano-fiber aggregation.
(5) above-mentioned hygrometric state silk fibroin nano-fiber aggregation is freeze-dried, obtains dry state silk fibroin nano-fiber.
Referring to attached drawing 1, it is the electromicroscopic photograph figure after the silk fibroin nano-fiber freeze-drying that the present embodiment is prepared.
Embodiment 2
(1) after pure fibroin fiber of the degumming after dry being dissolved and dialysed with the lithium bromide of 9.3M, fresh family is obtained
Fibroin protein solution, is cast in polystyrene mould, and sample path length is 3 millimeters, under 25 DEG C, 60% humidity environment quickly
Drying and moulding.
(2) rapid draing fibroin protein film after molding is impregnated 60 minutes in ethyl alcohol at room temperature, obtains recrystallization silk
Fibroin film.
(3) will recrystallization treated fibroin protein film in 1/50(w/v) ratio is soaked in 37 DEG C, 0.01M, pH=7.0
Dibastic sodium phosphate-phosphate sodium dihydrogen buffer solution in, soaking time 1h, then 3000 revs/min of 17 min of centrifugation, separation obtain
Supernatant.
(4) after supernatant achieved above being diluted to 0.25mg/ml, 6h is cultivated in water bath with thermostatic control environment, is obtained wet
State silk fibroin nano-fiber aggregation.
(5) above-mentioned hygrometric state silk fibroin nano-fiber aggregation is freeze-dried, obtains dry state silk fibroin nano-fiber.
Embodiment 3
(1) after pure fibroin fiber of the degumming after dry being dissolved and dialysed with the lithium bromide of 9.3M, fresh family is obtained
Fibroin protein solution, is cast in polystyrene mould, and sample path length is 2 millimeters, under 23 DEG C, 55% humidity environment quickly
Drying and moulding.
(2) rapid draing fibroin protein film after molding is impregnated 120 minutes in ethyl alcohol at room temperature, obtains recrystallization silk
Fibroin film.
(3) will recrystallization treated fibroin protein film in 1/30(w/v) ratio is soaked in 37 DEG C, 0.05M, pH=
In 7.0 tris- hydrochloride buffer, soaking time 0.5h, then 7000 revs/min of 10 min of centrifugation, separation obtain supernatant
Liquid.
(4) after supernatant achieved above being diluted to 0.75mg/ml, 3h is cultivated in water bath with thermostatic control environment, is obtained wet
State silk fibroin nano-fiber aggregation.
(5) above-mentioned hygrometric state silk fibroin nano-fiber aggregation is freeze-dried, obtains dry state silk fibroin nano-fiber.
Embodiment 4
(1) after pure fibroin fiber of the degumming after dry being dissolved and dialysed with the lithium bromide of 9.3M, fresh family is obtained
Fibroin protein solution, is cast in polystyrene mould, and sample path length is 1 millimeter, under 22 DEG C, 50% humidity environment quickly
Drying and moulding；(2) rapid draing fibroin protein film after molding is impregnated 90 minutes in ethyl alcohol at room temperature, obtains recrystallization silk
Fibroin film.
(3) will recrystallization treated fibroin protein film in 1/100(w/v) ratio is soaked in 37 DEG C, 0.01M, pH=7.0
Potassium hydrogen phosphate-potassium phosphate buffer in, soaking time 0.5h, then 4000 revs/min of 15 min of centrifugation, separation obtain
Obtain supernatant.
(4) after supernatant achieved above being diluted to 0.25mg/ml, 6h is cultivated in water bath with thermostatic control environment, is obtained wet
State silk fibroin nano-fiber aggregation.
(5) above-mentioned hygrometric state silk fibroin nano-fiber aggregation is freeze-dried, obtains dry state silk fibroin nano-fiber.
Claims (4)
1. a kind of preparation method of silk fibroin protein nanofiber obtains after degumming, drying process using silkworm silk as raw material
Pure fibroin fiber, it is characterised in that carry out following steps again:
(1) pure silk fibroin Fibrinolysis is obtained into bombyx mori silk fibroin in the lithium-bromide solution that concentration is 9.3 mol/L after dialysis
Protein solution is cast in mold, and under conditions of temperature is 20~25 DEG C, humidity is 45~65%, drying and moulding is thickness
0.5~5 millimeter of fibroin protein film；
(2) under room temperature, fibroin protein film is placed in ethyl alcohol and is impregnated 5~120 minutes, obtain the fibroin albumen of recrystallization
Film；
(3) fibroin protein film of recrystallization is soaked in the buffering that temperature is 25~50 DEG C by 1/100~1/10 w/v in proportion
In liquid, soaking time is 0.5~2 h；Again through 3000~10000 revs/min of centrifugal treatings, separation obtains supernatant；
(4) supernatant of acquisition is diluted to 0.1~10mg/ml, is cultivated in water bath with thermostatic control environment, obtain hygrometric state fibroin
Protein nano fibril aggregation object；
(5) hygrometric state silk fibroin nano-fiber aggregation is subjected to freeze-drying process, obtains silk fibroin protein nanofiber.
2. a kind of preparation method of silk fibroin protein nanofiber according to claim 1, it is characterised in that: described
Buffer is the trishydroxymethylaminomethane-hydrochloric acid or phosphate buffer of pH=7.0, and buffer concentration is 0.01~1 mol/
L。
3. a kind of preparation method of silk fibroin protein nanofiber according to claim 1, it is characterised in that: described
Water bath with thermostatic control environment, temperature are 25~50 DEG C；Incubation time is 3~8 h.
4. a kind of silk fibroin protein nanofiber obtained by claim 1 preparation method, diameter is 20~500 nm, length
It is 1~100 μm.
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| CN201611099113.7A CN106757447B (en) | 2016-12-04 | 2016-12-04 | A kind of silk fibroin protein nanofiber and preparation method thereof |
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| CN106757447B true CN106757447B (en) | 2019-06-18 |
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| CN109797447B (en) * | 2019-02-22 | 2021-06-29 | 上海交通大学 | Preparation method of zein nanofiber |
| CN114249982B (en) * | 2022-01-25 | 2023-05-09 | 武汉纺织大学 | Preparation method and application of high-strength high-modulus silk material |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008756A (en) * | 2010-12-10 | 2011-04-13 | 苏州大学 | Preparation method of nano-fibrous silk fibroin-based porous scaffold |
| CN103498210A (en) * | 2013-08-21 | 2014-01-08 | 浙江理工大学 | Biomimetic preparation method of high-strength regenerated silk protein fiber |
| CN103757729A (en) * | 2013-12-28 | 2014-04-30 | 复旦大学 | Silk protein nano-microfiber and preparation method thereof |
| CN105968384A (en) * | 2016-05-19 | 2016-09-28 | 苏州大学 | Amorphous silk fibroin nanofiber solution and preparation method thereof |
| CN106139246A (en) * | 2016-08-19 | 2016-11-23 | 苏州大学 | A kind of regenerated silk azelon support and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5257943B2 (en) * | 2009-05-25 | 2013-08-07 | 国立大学法人信州大学 | Method for producing silk protein nanofiber |
| KR101447256B1 (en) * | 2013-10-10 | 2014-10-08 | 경북대학교 산학협력단 | Process for preparing silk fibroin nano fiber |
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2016
- 2016-12-04 CN CN201611099113.7A patent/CN106757447B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008756A (en) * | 2010-12-10 | 2011-04-13 | 苏州大学 | Preparation method of nano-fibrous silk fibroin-based porous scaffold |
| CN103498210A (en) * | 2013-08-21 | 2014-01-08 | 浙江理工大学 | Biomimetic preparation method of high-strength regenerated silk protein fiber |
| CN103757729A (en) * | 2013-12-28 | 2014-04-30 | 复旦大学 | Silk protein nano-microfiber and preparation method thereof |
| CN105968384A (en) * | 2016-05-19 | 2016-09-28 | 苏州大学 | Amorphous silk fibroin nanofiber solution and preparation method thereof |
| CN106139246A (en) * | 2016-08-19 | 2016-11-23 | 苏州大学 | A kind of regenerated silk azelon support and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 家蚕丝素蛋白纳米纤维的调控及其在组织修复材料中的应用;白树猛;《中国博士学位论文全文数据库 基础科学辑》;20130915(第9期);摘要，正文第30-47、82-84页 |
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Inventor after: Lu Shenzhou Inventor after: Xing Tieling Inventor after: Yin Zhuping Inventor after: Wu Feng Inventor before: Yin Zhuping Inventor before: Lu Shenzhou Inventor before: Wu Feng Inventor before: Xing Tieling |
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Address after: 226300 New Century Avenue, Tongzhou District, Nantong, Jiangsu 266 Patentee after: NANTONG TEXTILE & SILK INDUSTRIAL TECHNOLOGY Research Institute Address before: 226004 Jiangsu Province, Nantong City Chongchuan District Chongchuan Road No. 58 Patentee before: NANTONG TEXTILE & SILK INDUSTRIAL TECHNOLOGY Research Institute |
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