CN1465395A - Productive process for glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor - Google Patents

Abstract

The production process of ganoderma cordyceps lung-boosting oral liquor includes the following procedures: (1) preparing ganoderma mycelium extract; (2). preparing cordyops fermented liquor; (3) preparing tremella extract; (4) preparing Chinese medicinal material extract; and (5). preparing oral liquor, and includes the following steps: mixing all the extracts, concentrating, blending, homogenizing, filling, capping, sterilizing, packaging, checking to obtain the invented finished prdouct with the functions of suppressing cough, transforming phlegm and raising immunity of human body.

Description

The production technology of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor

Technical field: the production technology that the present invention relates to a kind of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor.

Prior art: through consulting patent documentation, an at present domestic existing top is about " ganoderma lucidum cordyceps sinensis nutritional solution and preparation method thereof " (number of patent application: application for a patent for invention 96110518), the shortcoming that exists: the one, do not take biological fermentation engineering and zymolysis technique that raw material is handled, thereby can not fully extract the nutritional labeling of raw material, and the 2nd, the human lung there is not special efficacy.

Summary of the invention: the objective of the invention is to provide a kind of production technology of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor, the oral liquid that this technology is produced not only has cough-relieving, reduce phlegm and the effect of enhancing immunity, and chronic bronchitis is had preventive and therapeutic effect.

Raw material amount of dry matter proportioning of the present invention is: Ganoderma mycelium extracting solution 20-60%, Cordyceps fermented liquid 5-40%, Tremella 2-15%, Bulbus Fritillariae Cirrhosae 1-5%, Radix Ophiopogonis 1-5%, Herb Gynostemmae Pentaphylli 1-5%, Radix Pseudostellariae 1-5%, Radix Codonopsis 1-5%, Radix Glycyrrhizae 1-5%.

Production technology of the present invention is undertaken by following operation:

(1) Ganoderma mycelium extracting solution preparation:

1. Ganderma lucidum strain slant culture; 2. one-level is cultivated; 3. secondary is cultivated; 4. enzymolysis lixiviate; 5. filter; 6. it is standby to sterilize.

(2) preparation of Cordyceps fermented liquid:

1. Cordyceps slant culture; 2. shake-flask seed liquid is cultivated; 3. fermentation tank liquid culture; 4. twist body barreling mill; 5. enzymolysis processing; 6. it is standby to sterilize.

(3) tremella extract preparation:

1. Tremella making beating; 2. enzymolysis lixiviate; 3. filter standby.

(4) extracts of Chinese herbal medicine preparation:

1. Chinese herbal medicine is pulverized; 2. soak; 3. decoct and extract; 4. filter standby.

(5) production of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor:

1. Cordyceps fermented liquid, Tremella, extracts of Chinese herbal medicine are mixed with the Ganoderma mycelium extracting solution; 2. concentrate; 3. allotment; 4. homogenizing; 5. fill; 6. capping; 7. sterilization; 8. pack; 9. check; 10. finished product.

In the present invention, Ganoderma has the asthenia of controlling, cough, asthma, insomnia, dyspepsia, prevents and treats bronchial asthma, treats effects such as leukopenia, cardiovascular disease, acute infectious hepatitis; Cordyceps has strengthening the body resistance, effects such as tonification is strong, strengthening the spleen and stomach, tonifying the lung kidney, beneficial vital essence, the phthisical cough that reduces phlegm only; Tremella can be controlled cough due to consumptive disease, and deficiency-heat is thirsty, has nourishing YIN and moistening the lung, effects such as the cough-relieving of promoting the production of body fluid, pathogenic fire reducing, intestine moistening stomach reinforcing, beauty treatment skin moistening, life lengthening; Have Bulbus Fritillariae Cirrhosae, Radix Ophiopogonis effects such as preventing phlegm from forming and stopping coughing, clearing heat and moistening lung; Herb Gynostemmae Pentaphylli has effects such as nourishing, calmness, antitonic, short appetite, blood fat reducing, cholesterol reducing, transaminase lowering, slow down aging, anti-curing cancers to human body; Radix Pseudostellariae, Radix Codonopsis can tonifying the lung, spleen invigorating, control the deficiency of the lung, lung benefiting gas; Radix Glycyrrhizae energy and middle emergency, lung moistening, detoxifcation, coordinating the actions of various ingredients in a prescription can be controlled effects such as overstrain heating, consumptive lung disease cough.

In a word, the herbal raw material in the prescription all has strengthening the body resistance, nourishing YIN and moistening the lung, preventing phlegm from forming and stopping coughing, prevents and treats effects such as bronchial asthma, resisting fatigue, physical strength reinforcing, is collection health care and the natural conditioning nourishing treasure of treatment for one.In addition, among the preparation technology of the present invention, adopt Ganoderma mycelium extract, Cordyceps fermented liquid is a primary raw material, both had nutrition and the effect of natural glossy ganoderma, Cordyceps, reduced product cost again, be more suitable in industrialized great production, adopted zymolysis technique and high pressure homogenize to handle, can effectively extract active component, make the material granular, improve mouthfeel, thereby make nutrient substance more comprehensively, more help being absorbed by the body.

Embodiment:

One, the composition of raw materials of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor:

Press raw material dry matter weight proportioning, every 1000kg product: Ganoderma mycelium extracting solution 500kg, Cordyceps fermented liquid 300kg, Tremella 50kg, Bulbus Fritillariae Cirrhosae 30kg, Radix Ophiopogonis 30kg, Herb Gynostemmae Pentaphylli 30kg, Radix Pseudostellariae 20kg, Radix Codonopsis 20kg, Radix Glycyrrhizae 20kg.

Two, the production technology of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor:

1, the technological process of production:

(1) Ganoderma mycelium extracting solution preparation:

1. Ganderma lucidum strain slant culture; 2. one-level is cultivated; 3. secondary is cultivated; 4. enzymolysis lixiviate; 5. filter; 6. it is standby to sterilize.

(2) preparation of Cordyceps fermented liquid:

1. Cordyceps slant culture; 2. shake-flask seed liquid is cultivated; 3. fermentation tank liquid culture; 4. twist body barreling mill; 5. enzymolysis processing; 6. it is standby to sterilize.

(3) tremella extract preparation:

1. Tremella making beating; 2. enzymolysis lixiviate; 3. filter standby.

(4) extracts of Chinese herbal medicine preparation:

1. Chinese herbal medicine is pulverized; 2. soak; 3. decoct and extract; 4. filter standby.

(5) production of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor:

1. Cordyceps fermented liquid, Tremella, extracts of Chinese herbal medicine are mixed with the Ganoderma mycelium extracting solution; 2. concentrate; 3. allotment; 4. homogenizing; 5. fill; 6. capping; 7. sterilization; 8. pack; 9. check; 10. finished product.

2, key points for operation:

(1) Ganoderma mycelium extracting solution preparation:

Ganderma lucidum strain is provided by Institute of Microorganism, Academia Sinica, and its test tube strains culture medium prescription is: horse bell administration 20%, glucose 2.0%, KH ₂PO ₄0.2%, MgSO ₄0.05%, agar 2%.

The Ganoderma mycelium culture medium prescription is: carbohydrate 50-70%, protein 5-20%, fatty 1-2%, crude fibre 1-4%, ash 1-3%, sucrose 0.5-2%, CaSO ₄0.1-1%, KH ₂PO ₄0.1-1% and micro-Ca, P, Fe, vitamin.

Amylase (7658): food stage, enzyme activity 2000u/g, the prosperous and powerful biofermentation in Beijing welfare country produces.

Compound protease (distant sky 23): food stage, enzyme activity 3000u/g, Guangzhou distant sky enzyme preparation factory produces.

Fruit is twisted enzyme: food stage, Pectinex 5XL, Denmark Novo Nordisk Co.,Ltd produces.

Mycelium culture medium prescription raw material is mixed with water, through autoclaving (126 ℃, 1h) handle, activatory test tube strains is inserted in the cooling back, cultivation temperature is with 25 ℃--27 ℃ of scopes are more suitable.Temperature is low excessively, and mycelial growth is slow, the too high mycelial growth that also is unfavorable for of temperature.Mycelia requires healthy and strong strong, and color and luster is pure, pure white, glossy, has certain fragrant, does not have any living contaminants, and vitality is strong.After treating that mycelia is covered with culture medium, extract, and carry out enzymolysis processing (50 ℃, 2h, pH value 6.0), can obtain the Ganoderma mycelium extracting solution after enzyme denaturing, the filtration with the compound protease of the amylase of 0.01-0.5% and 0.01-0.5% with conventional extraction process by water.

(2) Cordyceps fermented liquid preparation:

Cordyceps strain is provided by Institute of Microorganism, Academia Sinica, and its culture medium prescription is as follows:

Cordyceps test tube strains culture medium: Rhizoma Solani tuber osi 20%, glucose 2.0%, agar 2%.

Cordyceps shake-flask seed culture medium: Semen Glycines powder 1-5%, sucrose 1-3%, KH ₂PO ₄0.01-5%, MgSO ₄0.01-0.1%.

Chinese caterpillar fungus fermentation jar culture medium: Semen Glycines powder 1-5%, sucrose 1-3%, KH ₂PO ₄0.01-5%, MgSO ₄0.01-0.1%.

Deep layer fermenting process key points for operation: a, shake-flask seed are cultivated and are adopted rotary shaking table, and 100 times/min of shaking table frequency cultivated 4-7 days, form shake-flask seed.Inoculum concentration 5-15%, through 4-5 days, after the 25-27 ℃ of constant temperature culture, fermentation liquid was pale yellow little turbid, formed the mycelium pellet of the about 0.5-1.5mm size of diameter, and fermentation liquid has a kind of inherent delicate fragrance flavour of a drug.It is 25-27 ℃ that optimum temperature is cultivated in b, Cordyceps submerged fermentation.When temperature was lower than 25 ℃, mycelial growth obviously was suppressed; When being higher than 28 ℃, the easy old and feeble self-dissolving of mycelial growth, culture fluid darkens.C, Cordyceps deep layer are cultivated and are required to have a meta-acid environment, and this has considered when selecting culture medium constituent, has not needed to test specially pH value during preparation.D, ventilation are cultivated very important to the Cordyceps deep layer, general ventilation is with 1: 0.5-1: 1.0/min is advisable.Bubbling air is too much or very few, all is unfavorable for mycelial growth.E, ventilation deep layer are cultivated jar standard of putting: hyphae content 〉=15% (weight in wet base); Abnormal smells from the patient delicate fragrance; Residual total sugar is reduced to below the 10mg/ml; Fermentation liquid is thickness very, shape such as paste; The mycelium aging, minority self-dissolving, cultivation cycle 3-6 days.F, the mycelium process is broken extracts active substance by modern biotechnology.

(3) tremella extract preparation:

Select free from admixture Tremella fructification dry product for use, by 1: 50-100 water addition ratio example, at room temperature soak 30min, put fully fragmentation in the high speed beater, make the Tremella serosity.PH transfers to 6.0-7.0 with the Tremella serosity, adds the 0.5-1.5% compound enzymic preparation, and the control temperature is between 50 ℃-55 ℃, and enzymolysis 60min is warming up to enzyme denaturing more than 85 ℃ then, and hot water lixiviate two times merges lixiviating solution, filters standby.

(4) Chinese herbal medicine extracting:

Other herbal raw material pulverize separately is a diameter 0.5-1cm granule in will filling a prescription, and drops in the extraction pot, adds 2-10 times of water logging bubble 2-3h, hot water decocts 30-90min then, and second time extraction adds 2-5 times of decocting again and boil 30-60min in medicinal residues, merge secondary raffinate, filter standby.

(5) allotment concentrates:

Ganoderma mycelium extracting solution, Cordyceps fermented liquid, Tremella and extracts of Chinese herbal medicine are mixed in proportion, inject the cryogenic vacuum concentration tank and concentrate.Being concentrated into soluble solid is more than the 5-15% (20 ℃ of refractometers are measured).

(6) allotment homogenizing:

Feed liquid is handled through the 50Mpa high pressure homogenizer, makes the material granular, and diameter is the micromolecule of 0.2-1 μ m, can effectively solve the oral liquid sedimentation problem and improve mouthfeel.

(7) fill sterilization:

With the feed liquid brown feeding solution fill of 250ml, 100ml, capping behind the allotment homogenizing, bactericidal formula is: 30 '-40 '-30 '/118 ℃, and through packing, be after the assay was approved finished product.

Glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor of the present invention is analyzed after tested and is contained rich nutrient contents and special healthy ingredient.Show to have significant cough-relieving, reduce phlegm and the enhancing immunity function, and chronic bronchitis is had certain preventive and therapeutic effect through function test, and safety non-toxic.

Claims (3)

1, a kind of production technology of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor is characterized in that: raw material dry matter weight proportioning is: Ganoderma mycelium extracting solution 20-60%, Cordyceps fermented liquid 5-40%, Tremella 2-15%, Bulbus Fritillariae Cirrhosae 1-5%, Radix Ophiopogonis 1-5%, Herb Gynostemmae Pentaphylli 1-5%, Radix Pseudostellariae 1-5%, Radix Codonopsis 1-5%, Radix Glycyrrhizae 1-5%.

2, the production technology of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor according to claim 1 is characterized in that, is undertaken by following operation:

(1) Ganoderma mycelium extracting solution preparation:

1. Ganderma lucidum strain slant culture; 2. one-level is cultivated; 3. secondary is cultivated; 4. enzymolysis lixiviate; 5. filter; 6. it is standby to sterilize.

(2) preparation of Cordyceps fermented liquid:

1. Cordyceps slant culture; 2. shake-flask seed liquid is cultivated; 3. fermentation tank liquid culture; 4. twist body barreling mill; 5. enzymolysis processing; 6. it is standby to sterilize.

(3) tremella extract preparation:

1. Tremella making beating; 2. enzymolysis lixiviate; 3. filter standby.

(4) extracts of Chinese herbal medicine preparation:

1. Chinese herbal medicine is pulverized; 2. soak; 3. decoct and extract; 4. filter standby.

(5) production of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor:

1. Cordyceps fermented liquid, Tremella, extracts of Chinese herbal medicine are mixed with the Ganoderma mycelium extracting solution; 2. concentrate; 3. allotment; 4. homogenizing; 5. fill; 6. capping; 7. sterilization; 8. pack; 9. check; 10. finished product.

3, the production technology of glossy ganoderma and cordyceps sinensis lung-reinforcing oral liquor according to claim 1 is characterized in that:

In the Ganoderma mycelium extracting solution preparation section:

Its test tube strains culture medium prescription is: Rhizoma Solani tuber osi 20%, glucose 2.0%, KH ₂PO ₄0.2%, MgSO ₄0.05%, agar 2%.

The Ganoderma mycelium culture medium prescription is: carbohydrate 50-70%, protein 5-20%, fatty 1-2%, crude fibre 1-4%, ash 1-3%, sucrose 0.5-2%, CaSO ₄0.1-1%, KH ₂PO ₄0.1-1% and micro-Ca, P, Fe, vitamin.