CN1460411A - Quick breeding method of foliage plant heterochromous colocasia - Google Patents
Quick breeding method of foliage plant heterochromous colocasia Download PDFInfo
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- CN1460411A CN1460411A CN 03135144 CN03135144A CN1460411A CN 1460411 A CN1460411 A CN 1460411A CN 03135144 CN03135144 CN 03135144 CN 03135144 A CN03135144 A CN 03135144A CN 1460411 A CN1460411 A CN 1460411A
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Abstract
The present invention provides a quick breeding method of foliage plant heterochromous taro. The tufted bud induction culture medium MS+BA 5mg/L+NAA 0-0.1 mg/L, the rooting culture medium is 1/2 MSNAA mg/L, and the quick breeding process is as follows: heterochromous taro tuber mail bud and stole axially bud-obtaining tufted bud from tufted bud induction culture medium-MS+BA 1-2 mg/L+NAA 0.5 mg/L for strengthening seedling-making induction in rooting culture medium to root. Its monthly multiplication rate is 1:4, and its whole reproductive cycle is five months.
Description
Technical field: the invention belongs to biological technical field, relate to the method for quickly breeding of the heterochromatic taro of a kind of ornamental foliage plant particularly.
Background technology: heterochromatic taro (Colocasia heterochroma H.Li et Z.X.Wei) is the taro genus novel species that Lee found and named in identical 1991.It is distributed in the dark and damp place at height above sea level 1100m place, the west and south, Yunnan, about plant height 20cm.Heterochromatic taro blade peltate and is given birth to, and the heart of ovum shape is membranous, long 12cm, wide 10cm.This kind leaf green is blackish green or mulberry between rib and lateral vein, sees that far the blade face is velvet-like, very beautiful like swan, can be used as after transforming and sees the leaf pot plant.But because extremely limit to its area, add the main vegetative propagation by stolon of breeding, natural renewal speed is extremely slow, because its habitat has been subjected to bigger destruction, adds arbitrarily and excavates, and has been difficult to find wild plant at present.
Summary of the invention: the objective of the invention is at prior art problems, heterochromatic taro is carried out tissue culture and the research of breeding fast, the method for quickly breeding of the heterochromatic taro of a kind of ornamental foliage plant is provided, set up fast numerous technology, for the exploitation and this wild resource of sustainable utilization new propagation technique is provided, and for the creation new varieties increased a new starting material.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
The method for quickly breeding of the heterochromatic taro of a kind of ornamental foliage plant, get wild heterochromatic taro stem tuber main bud, stolon axillalry bud, adopt that explant sterilization back inserts that the clump bud that contains different plant hormones is induced, after strong sprout, root media, be transplanted into plantation hardening matrix, clump bud inducing culture is MS+BA3-5mg/L+NAA0-1mg/L, the strong seedling culture base is MS+BA1-2mg/L+BAA0.5mg/L, root media is 1/2MS.NAA1mg/L, agar 6.2g/L, sucrose 30g/L, pH value 5.8,27 ± 2 ℃ of cultivation temperature, light application time 12h/d, intensity of illumination 2000LX.
The sterilization of said method explant rinses out the earth of being with on the material with running water earlier, removes the brown crust of stem tuber and stolon co-fibration, and the alcohol with 75% is handled 10s, uses 0.1%HgCl again
2Sterilization 20-30min, aseptic water washing 5min flushing 2-3 time is blotted the moisture of be with on the material with aseptic paper, the access of finishing back went out bacterium with the blake bottle of moisture, carry out two-stage sterilization about 6d again, difference cut-off blade, petiole, bud and stem are in the access medium.
The loess of soil layer depths and leaf mould were in 1: 1 ratio mixing when said method plantation hardening matrix was dug ground for using, sterilize with 1/20 formalin, sealing 24h, open periphery and cover plastic film, just can adorn basket about about 6d, standby for transplanting seedlings, the transplanting of said test-tube plantlet is for working as the complete about 3-5cm of test tube height of seedling, tool 3-5 sheet leaf and about 3 of roots are arranged, when being about 1cm, just can transplant, be planted on the previous sterile matrix, open and cultivate bottleneck envelope film, do not damage when getting the seedling blade or the tip of a root, the conscientious careful agar of being with on the clean root system of removing just can be transplanted seedlings, irrigate normal root water, note preserving moisture and preventing the sunlight direct projection, will note ventilation, treat that test-tube plantlet grows young leaves at the 3d that transplants seedlings, can go out and cover the plastic foil of preserving moisture, low level management.
Embodiment:
Further flesh and blood of the present invention is described below in conjunction with embodiments of the invention, but content of the present invention is not limited thereto.
Embodiment 1:
1 materials and methods
1.1 the wild heterochromatic taro stem tuber main bud of material, stolon axillalry bud
1.2 method
1.2.1 explant sterilization because of heterochromatic taro for a long time by asexual multiplication of future generations, stem tuber and stolon are grown in soil again, institute be with the bacterium of mixing more, it is not thorough to sterilize with conventional method, causes waste of material and test failure.For addressing this problem, after the present invention gathers explant, rinse out the earth of being with on the material with running water earlier, remove the brown crust (noting not damaging interior tissue) of stem tuber and stolon co-fibration, the alcohol with 75% is handled 10s, uses 0.1%HgCl again
2Sterilization 20-30min, aseptic water washing 5min flushing 2-3 time is blotted the moisture of being with on the material with aseptic paper, finishing back insert the bacterium of going out with the blake bottle of moisture, carry out two-stage sterilization (method is the same) about 6d again, cut-off blade, petiole, bud and stem insert in the medium respectively.
1.2.2 condition of culture clump bud inducing culture is MS+BA3-5mg/L (unit down together)+NAA0-1 (seeing table 1 for details), the strong seedling culture base is MS+BA1-2+BAA0.5, root media is 1/2MS.NAA1, agar 6.2g/L, sucrose 30g/L, pH value 5.8,27 ± 2 ℃ of cultivation temperature, light application time 12h/d, intensity of illumination 2000LX.
1.2.3 plant the loess plant hardening matrix hardening matrix soil layer depths when digging ground and leaf mould in 1: 1 ratio mixing, with the sterilization of 1/20 formalin, sealing 24h opens periphery covering plastic film, just can adorn basket about about 6d, it is standby to supply to transplant seedlings.
2 experimental results
2.1 the clump bud is induced
As shown in Table 1, the only explant of heterochromatic taro clump bud is stem tuber main bud and stolon axillalry bud.Only medium is MS+BA5+NAA0-0.1, and month rate of increase is 1: the less intensive lumps that is of clump bud that 4-5, MS+BA5 induce, the bud size is about 0.2cm, can cut subculture, but propagation must be through the stage in strong sprout (MS+BA1-2+NAA0.2-0.5), bud just can be grown up.Petiole and root segment are cultivated, and through observation in 5 months, do not induce clump bud, and base portion also can produce downright bad phenomenon in incubation.
The growth response explant medium upgrowth situation of table 1 different explants on medium
MSBA3 51d bastem portion long incision clump bud
MSBA5 26d bastem portion otch has about 20 the long roots of stem tuber main bud MSBA5NAA1 45d bastem portion's otch clump bud of intensive clump bud
MSBA5NAA0.1 43d bastem portion otch has about 10 of clump bud
MSBA5IBA0.1 43d bastem portion otch has about 6 of clump bud
MSBA3 71d bastem portion long incision simple bud stolon axillalry bud
MSBA5NAA0.05 71d bastem portion long incision is slowly grown root white from bud, callus root MSBA4NAA0.05 90d root
MSBA3 20d leaf is grown up, is wrinkled, and few light green callus leaf texture appears in 30d
MSBA4NAA0.05 23d leaf is grown up, is wrinkled, and the light green callus appears in otch
MS 15d does not have significant change
MSBA4NAA0.05 23d incision is expanded, and long undeveloped callus petiole MSBA5 60d incision is expanded, long undeveloped callus
MSBA5NAA1 60d incision is expanded, long undeveloped callus
MSBA5NAA0.05 23d incision is expanded, long undeveloped callus
2.2 the heterochromatic taro clump of root induction bud can be cut into simple bud, the bud height is placed on root induction on the medium of 1/2MS+NAA1 when 1-2cm, cultivate through 20-25d, take root more than 90%, every young plant can grow several roots, when root grows 1cm, there is the seedling of 2-3 sheet leaf just can transplant.
2.3 the transplanting of test-tube plantlet is when the complete about 3-5cm of test tube height of seedling, tool 3-5 sheet leaf and about 3 of roots are arranged, when being about 1cm, just can transplant, and is planted on the previous sterile matrix.Open and cultivate bottleneck envelope film, do not damage when getting the seedling blade or the tip of a root, the conscientious careful agar of being with on the clean root system of removing just can be transplanted seedlings, and irrigates normal root water, note preserving moisture and preventing the sunlight direct projection, to note ventilation at the 3d that transplants seedlings, treat that test-tube plantlet grows young leaves, can go out and cover the plastic foil of preserving moisture, low level management, transplanting survival rate 90%.
3 discuss
In the tissue culture of heterochromatic taro, clump bud inducing culture is MS+BA5mg/L+NAA0-0.1mg/L, and root media is 1/2MSNAAmg/L.Fast the breeding program is: heterochromatic taro stem tuber main bud and the stolon axillalry bud → bud that on clump bud inducing culture, obtains growing thickly → MS+BA1-2mg/L+NAA0.5mg/L strong sprout → and in the root media root induction.Month rate of increase 1: 4, about 5 months of whole cyclostage.
The result shows, the undercut section is only slowly growth in cultivation, blade segment and petiole segment also only grow underdeveloped callus, there is not the regeneration of indefinite bud, possible cause is that condition of culture is improper, or monocotyledon specialization organ culture difficulty is bigger, due to required cultivation cycle is longer, tests still underway.
Heterochromatic taro stem tuber main bud was through 4 months tissue culture, find to have between the clump bud the unknown bacterium of several orange red 1-2mm size round point shapes to overflow clump bud or occur in the one side of clump bud contact medium, can remove this bacterium is transferred on the fresh culture, the clump bud still can continued growth, but there is new roundlet point-like bacterium to occur, as if the white Acarasiales that the 5min material that goes out with 0.1%HGCL2 occurs, material is ruined entirely soon.These have the color bacterium might be saccharomycete, has to be identified and discussion to prevent and treat method.In the training of heterochromatic taro group, the BA5NAA0.1 combination is better than the BA5IBA0.1 combination, and the former is all favourable to clump bud propagation and growth.If in the taro bud was cultivated, bud was in the deadtime that has approximately on the medium about 30d; Just produce simple bud, clump bud then in bastem portion.
Claims (3)
1, the method for quickly breeding of the heterochromatic taro of a kind of ornamental foliage plant, get wild heterochromatic taro stem tuber main bud, the stolon axillalry bud, adopting explant sterilization back to insert the clump bud that contains different plant hormones induces, strong sprout, behind the root media, be transplanted into plantation hardening matrix, it is characterized in that clump bud inducing culture is MS+BA3-5mg/L+NAA0-1mg/L, the strong seedling culture base is MS+BA1-2mg/L+BAA0.5mg/L, root media is 1/2MS.NAA1mg/L, agar 6.2g/L, sucrose 30g/L, pH value 5.8,27 ± 2 ℃ of cultivation temperature, light application time 12h/d, intensity of illumination 2000LX.
2, method according to claim 1 is characterized in that said explant sterilization rinses out the earth of being with on the material with running water earlier, removes the brown crust of stem tuber and stolon co-fibration, and the alcohol with 75% is handled 10s, uses 0.1%HgCl again
2Sterilization 20-30min, aseptic water washing 5min flushing 2-3 time is blotted the moisture of be with on the material with aseptic paper, the access of finishing back went out bacterium with the blake bottle of moisture, carry out two-stage sterilization about 6d again, difference cut-off blade, petiole, bud and stem are in the access medium.
3, method according to claim 1, it is characterized in that said plantation hardening matrix for the loess of soil layer depths when digging ground and leaf mould in 1: 1 ratio mixing, sterilize with 1/20 formalin, sealing 24h, open periphery and cover plastic film, just can adorn basket about about 6d, standby for transplanting seedlings, the transplanting of said test-tube plantlet is for working as the complete about 3-5cm of test tube height of seedling, tool 3-5 sheet leaf and about 3 of roots are arranged, when being about 1cm, just can transplant, be planted on the previous sterile matrix, open and cultivate bottleneck envelope film, do not damage when getting the seedling blade or the tip of a root, the conscientious careful agar of being with on the clean root system of removing, just can transplant seedlings, irrigate normal root water, note preserving moisture and preventing the sunlight direct projection, 3d will note ventilation transplanting seedlings, treat that test-tube plantlet grows young leaves, can go out and cover the plastic foil of preserving moisture, low level management.
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| CN 03135144 CN1460411A (en) | 2003-06-04 | 2003-06-04 | Quick breeding method of foliage plant heterochromous colocasia |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1316877C (en) * | 2005-01-24 | 2007-05-23 | 黎建力 | Tissue culturing and breeding method for wild sea chestnut |
| CN102273406A (en) * | 2010-06-12 | 2011-12-14 | 上海上房园艺有限公司 | Method for culturing red-petiole taros |
| CN102763597A (en) * | 2012-08-15 | 2012-11-07 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103070078A (en) * | 2013-02-07 | 2013-05-01 | 江苏省农业科学院 | Rapid propagation method for performing tissue culture by using taro stem tip |
| CN112106665A (en) * | 2020-10-28 | 2020-12-22 | 中国科学院昆明植物研究所 | Culture medium for one-step seedling formation of amorphophallus rivieri and application and tissue culture method |
-
2003
- 2003-06-04 CN CN 03135144 patent/CN1460411A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1316877C (en) * | 2005-01-24 | 2007-05-23 | 黎建力 | Tissue culturing and breeding method for wild sea chestnut |
| CN102273406A (en) * | 2010-06-12 | 2011-12-14 | 上海上房园艺有限公司 | Method for culturing red-petiole taros |
| CN102273406B (en) * | 2010-06-12 | 2013-03-06 | 上海上房园艺有限公司 | Method for culturing red-petiole taros |
| CN102763597A (en) * | 2012-08-15 | 2012-11-07 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102763597B (en) * | 2012-08-15 | 2013-11-20 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
| CN103070078A (en) * | 2013-02-07 | 2013-05-01 | 江苏省农业科学院 | Rapid propagation method for performing tissue culture by using taro stem tip |
| CN112106665A (en) * | 2020-10-28 | 2020-12-22 | 中国科学院昆明植物研究所 | Culture medium for one-step seedling formation of amorphophallus rivieri and application and tissue culture method |
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