CN1452915A - Prepn of pumpkin seed essence with rich citrulline and lipoid - Google Patents
Prepn of pumpkin seed essence with rich citrulline and lipoid Download PDFInfo
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- CN1452915A CN1452915A CN03126659A CN03126659A CN1452915A CN 1452915 A CN1452915 A CN 1452915A CN 03126659 A CN03126659 A CN 03126659A CN 03126659 A CN03126659 A CN 03126659A CN 1452915 A CN1452915 A CN 1452915A
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Abstract
The preparation process of pumpkin seed essence containing rich citrulline and lipoid as antiproliferative factors from pumpkin seed kernel embryonic cell includes the steps of sorting pumpkin seed kernel, washing, enzyme deactivation, cell breaking, separating residue from slurry, blending, emulsifying, homogenization, spray drying or freeze drying, etc. The technological process can reserve citrulline and lipoid as antiproliferative factors contained against damage and oxidation. The produced pumpkin seed essence may be used further in producing medicine, health beverage and food for preventing and treating prostatosis.
Description
Technical field
The present invention relates to a kind of pumpkin seeds essence manufacture method that is rich in anti-hyperplasia factor citrulling and lipid of from pumpkin seeds benevolence blastocyte, extracting.
Background technology
Pumpkin, its kind are divided into musky gourd (Cucurbita moschata Duch), american pumpkin (Cucurbita pepo L.), 3 cultigens of giant pumpkin (Cucurbita maxima Duch.et Lam), belong to Curcurbitaceae annual herb plant.Discover that contained anti-hyperplasia factor citrulling and lipid comprise that to preventing and treating prostatic disorders prostatitis, prostate swelling, hypertrophy of the prostate, hyperplasia of prostate, effect on prostate carcinoma are obvious in the pumpkin seeds benevolence blastocyte.From pumpkin seeds, obtain anti-hyperplasia factor citrulling and lipid correlation technique, become the research and development focus of medicine and health industry in recent years as medical material or functional health-care food.
Summary of the invention
The purpose of this invention is to provide a kind ofly has the pumpkin seeds essence manufacture method of unique effects to preventing and treating prostatic disorders, this method technology is simple, can effectively keep contained anti-hyperplasia factor citrulling and lipid is not damaged and oxidation in process, effectively prolong the finished product storage life, the pumpkin seeds essence that is obtained can further be processed into the control in prostatic disorders of medicine, health drink or food applications.
Method of the present invention is that pumpkin seeds benevolence is separated, allocated emulsification homogeneous and operations such as spray-drying or freeze-drying through screening, cleaning, enzymatic inactivation, clasmatosis, screenings, obtains being rich in the pumpkin seeds essence of anti-hyperplasia factor citrulling and lipid.The pumpkin seeds essence that is obtained also can further be processed into the control in prostatic disorders of medicine, health drink or food applications, so that the medical value of pumpkin seeds is more widely used.
The concrete steps of the inventive method are as follows:
One, screening, cleaning, enzymatic inactivation: get the maturation pumpkin seeds benevolence (comprising musky gourd, american pumpkin, giant pumpkin) of having peeled off, reject flat grain, broken lobe grain, the grain of damaging by worms, screening mature and plump, shape are complete, the pumpkin seeds benevolence of no disease and pest.Clean through the clear water rinsing, place 75～95 ℃ of hot-water soak grooves to soak then and scald 1～10min, lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
Two, clasmatosis, screenings separate: adopt common cell breaking plant with the fragmentation of pumpkin seeds benevolence, the same category of device that also can adopt grinding mill, colloid mill, bruisher or have the smudge cells function of milling; Add pure water before and after the clasmatosis, addition is 0.5～3 times of pumpkin seeds benevolence weight.(the separator rotating speed is 3000～20000r/min) usually, separates the pumpkin seeds benevolence emulsion that obtains to be rich in anti-hyperplasia factor citrulling and lipid again will the pumpkin seeds benevolence after pulverizing to put into screenings separator or centrifuge or filter separating and filtering.
Three, allotment emulsification homogeneous and spray-drying: allotment emulsification homogeneous adopts Homogenizing pump homogeneous or vacuum emulsification equipment emulsification (pressure 200～1000kg/cm usually
2Or speed of agitator 1000～20000r/min); Can also select to add emulsifying agent, flavor enhancement, thickener before allocating the emulsification homogeneous or behind the emulsification homogeneous according to the needs of design; But spray-drying working pressure spray-drying or centrifugal spray drying (pressure 100～600kg/cm
2, centrifugal rotational speed 10000～30000r/min, spray-drying hot-wind inlet temperature is 160～190 ℃, outlet temperature is 70～85 ℃); Also can adopt common industrial vacuum freeze drying plant to carry out drying.
The emulsifying agent that the present invention relates to is according to the needs of design, can select following one or more: single palmitic acid sorb glucosides ester, Emulsifier LT-60M, xylitan monostearate, glycerol monostearate acid, tripalmitin, cephalin, lipositol, glycophospholipin, lecithin, sorbitan mono-oleic acid ester, sorbitol anhydride tristearate, sorbitan monostearate, sucrose fatty ester, hydrogenated rosin glyceride, propylene glycol ester etc., the consumption of emulsifying agent is 0.000004～0.1% (weight ratio) of pumpkin seeds benevolence weight.
The flavor enhancement that the present invention relates to can be according to the design needs, select following one or more: sodium chloride, potassium chloride, sucrose, fructose, fruit glucose, glucose, maltose, compound sugar, xylo-oligosaccharide, oligoisomaltose, beet sugar, maltitol, isomerizing lactose, stevia, Aspartame, albumen sugar, acetic acid, citric acid, malic acid, tartaric acid, sodium phosphate etc., the consumption of flavor enhancement is 0.00004～20% (weight ratio) of pumpkin seeds benevolence weight.
The thickener that the present invention relates to is according to the needs of design, can select following one or more: methylcellulose, CMC, ethyl cellulose, HYDROXY PROPYL METHYLCELLULOSE and microcrystalline cellulose, starch, converted starch, dextrin, maltodextrin, Sodium Hydroxymethyl Stalcs, edible gelatin, Sodium Caseinate, vegetable protein, animal protein, Arabic gum, sesbania gum, pectin, agar, locust bean gum, hope Luo Zi glue, xanthan gum, the marine alga propylene glycol ester, alginates, carragheen, guar gum, bassora gums etc., the consumption of thickener are 0.00004～44% (weight ratio) of pumpkin seeds benevolence weight.
The inventive method can effectively be obtained preventing and treating pumpkin seeds anti-hyperplasia factor citrulling and the lipid that prostatic disorders have unique effects, and be processed into the pumpkin seeds essence that the back forms good instant emulsion and tasty milk mouthfeel that reconstitutes with higher commercial value, pumpkin seeds utilization rate and recovery rate height, the added value of product height, grease oxidation rancid does not appear in the product storage life, water-oil separating do not occur, and can effectively keep contained citrulling and lipid functional activity composition is not damaged; The pumpkin seeds essence of the inventive method manufacturing has the peculiar fragrance of pumpkin seeds benevolence.The pumpkin seeds essence also can be used as medical material or is processed into the milk health drink or the food of good to eat delicious food.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1, gets the ripe pumpkin seeds benevolence 50kg that has peeled off, the pumpkin seeds benevolence of, free from insect pests complete and disease and pest, no flat grain through screening full grains, shape, clean through the clear water rinsing, place 75 ℃ of hot-water soak grooves to soak and scald 10min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, add pure water 100kg, after cell breaking plant is milled into slurry with the fragmentation of pumpkin seeds benevolence, obtain emulsion, again by Homogenizing pump emulsifying (pressure 200kg/cm by the screenings separator
2).
3,, obtain to be rich in the pumpkin seeds essence of citrulling and lipid through centrifugal spray drying (centrifugal rotational speed 10000r/min, the hot-wind inlet temperature is 160 ℃, outlet temperature is 80 ℃).
Embodiment two:
1, the fresh no flat grain of screening the pumpkin seeds benevolence 100kg that peels off, clean through the clear water rinsing.Place 95 ℃ of hot-water soak grooves to soak and scald 1min, pick up standby.
2, will add pure water 100kg through the pumpkin seeds benevolence of blanching, and be milled into slurry, use the filter separating and filtering, obtain pumpkin seeds benevolence elite emulsion with grinding mill.
3, allotment adds emulsifier lecithin 0.01kg, by the emulsification of vacuum emulsification equipment (rotating speed 10000r/min), through vacuum freeze drying, obtains to be rich in the pumpkin seeds essence of citrulling and lipid.
Embodiment three:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, the flat grain and the grain of damaging by worms are rejected in screening, and clear water is rinsed well.Place 80 ℃ of hot water of soaking compartment to soak and scald 7min.
2, add pure water 150kg and in colloid mill, be milled into slurry, separate screenings (rotating speed 3000r/min) through centrifuge.
3, the allotment of pumpkin seeds benevolence emulsion is added thickener converted starch 5kg, by homogeneous (pressure 800kg/cm
2), through pressure spray dryer (pressure 200kg/cm
2, 190 ℃ of hot-wind inlet temperature, 85 ℃ of outlet temperatures), the pumpkin seeds essence of citrulling and lipid is rich in acquisition.
Embodiment four:
1, the pumpkin seeds benevolence 100kg that peels off of the broken lobe grain of the fresh nothing of screening and the grain of damaging by worms is clean through the clear water rinsing.Place 90 ℃ of hot-water soak grooves to soak and scald 2min.
2, will add pure water 200kg through the pumpkin seeds benevolence of blanching, and smash the pulp thing to pieces, with filter separating and filtering (rotating speed 5000r/min) with bruisher.
3, allotment adds the acid of emulsifying agent glycerol monostearate 0.005kg, lecithin 0.005kg, flavor enhancement compound sugar 5kg, by the emulsification of vacuum emulsification equipment (rotating speed 2000r/min), through centrifugal spray drying (centrifugal rotational speed 15000r/min, 180 ℃ of hot-wind inlet temperature, 78 ℃ of outlet temperatures), obtain to be rich in the pumpkin seeds essence of citrulling and lipid.
Embodiment five:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, the flat grain and the grain of damaging by worms are rejected in screening, and clear water is rinsed well.Place 82 ℃ of hot water of soaking compartment to soak and scald 7min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, will add pure water 250kg through the pumpkin seeds benevolence of blanching and in colloid mill, be milled into slurry, separate screenings (rotating speed 5800r/min) through centrifuge.
3, pumpkin seeds benevolence emulsion is passed through allotment emulsification homogeneous (pressure 300kg/cm
2), add flavor enhancement xylo-oligosaccharide 15kg then, through pressure spray dryer (pressure 300kg/cm
2, 170 ℃ of hot-wind inlet temperature, 82 ℃ of outlet temperatures), the pumpkin seeds essence of citrulling and lipid is rich in acquisition.
Embodiment six:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, screening mature and plump, the pumpkin seeds benevolence that shape is complete are rinsed well through clear water.Place 88 ℃ of hot water of soaking compartment to soak and scald 5min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, will add pure water 180kg through the pumpkin seeds benevolence of blanching and be milled into slurry, install separating and filtering screenings (rotating speed 8000r/min) after filtration with grinding mill.
3, pumpkin seeds benevolence emulsion is added flavor enhancement oligoisomaltose 20kg, emulsifying agent sucrose fatty ester 0.0001kg, by Homogenizing pump emulsifying (pressure 600kg/cm
2), through centrifugal spray drying (centrifugal rotational speed 20000r/min, 165 ℃ of hot-wind inlet temperature, 72 ℃ of outlet temperatures), obtain to be rich in the pumpkin seeds essence of citrulling and lipid.
Embodiment seven:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, the pumpkin seeds benevolence of the no disease and pest of screening is rinsed well through clear water.Place 85 ℃ of hot water of soaking compartment to soak and scald 6min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, will add pure water 300kg through the pumpkin seeds benevolence of blanching and be milled into slurry through colloid mill, and through screenings separator separating emulsion (rotating speed 10000r/min).
3, pumpkin seeds benevolence emulsion is passed through vacuum emulsification equipment emulsification (rotating speed 5000r/min), add thickener maltodextrin 44kg,, obtain to be rich in the pumpkin seeds essence of citrulling and lipid through vacuum freeze drying.
Embodiment eight:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, the pumpkin seeds benevolence that screening is rejected flat grain and damaged by worms harmful is rinsed well through clear water.Place 93 ℃ of hot water of soaking compartment to soak and scald 2min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, will add pure water 80kg through the pumpkin seeds benevolence of blanching, and smash to pieces, filter, obtain to be rich in the pumpkin seeds benevolence emulsion of anti-hyperplasia factor citrulling and lipid with filter with bruisher.
3, pumpkin seeds benevolence emulsion is added thickener Sodium Caseinate 10kg, by Homogenizing pump emulsifying (pressure 800kg/cm
2), through pressure spray dryer (pressure 400kg/cm
2, the hot-wind inlet temperature is 168 ℃, outlet temperature is 73 ℃), the pumpkin seeds essence of citrulling and lipid is rich in acquisition.
Embodiment nine:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, the pumpkin seeds benevolence that screening is rejected flat grain and damaged by worms harmful is rinsed well through clear water, places 84 ℃ of hot water of soaking compartment to soak and scalds 6.5min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, will add pure water 120kg through the pumpkin seeds benevolence of blanching, and in colloid mill, be milled into slurry, separate screenings (rotating speed 8000r/min) through centrifuge.
3, pumpkin seeds benevolence emulsion is added emulsifying agent sucrose fatty ester 0.01kg, thickener maltodextrin 10kg, by the emulsification of vacuum emulsification equipment (rotating speed 1000r/min), through centrifugal spray drying (centrifugal rotational speed 18000r/min, the hot-wind inlet temperature is 176 ℃, outlet temperature is 82 ℃), the pumpkin seeds essence of citrulling and lipid is rich in acquisition.
Embodiment ten:
1, get the fresh maturation pumpkin seeds benevolence 100kg that peeled off, the screening mature and plump is also rejected the pumpkin seeds benevolence of flat grain, rinses well through clear water.Place 88 ℃ of hot water of soaking compartment to soak and scald 3min, the lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte.
2, will add pure water 200kg through the pumpkin seeds benevolence of blanching, and in bruisher, smash the pulp thing to pieces, separate (rotating speed 10000r/min), obtain pumpkin seeds benevolence extraction emulsion through centrifuge.
3, pumpkin seeds benevolence emulsion is added emulsifying agent glycophospholipin 0.08kg, by Homogenizing pump emulsifying (pressure 600kg/cm
2), through pressure spray dryer (pressure 300kg/cm
2, the hot-wind inlet temperature is 180 ℃, outlet temperature is 81 ℃), the pumpkin seeds essence of citrulling and lipid is rich in acquisition.
Claims (3)
1. one kind is extracted the pumpkin seeds essence manufacture method that is rich in anti-hyperplasia factor citrulling and lipid from pumpkin seeds benevolence blastocyte, it is characterized in that pumpkin seeds benevolence is separated, allocated emulsification homogeneous, spray-drying or freeze-drying through screening, cleaning, enzymatic inactivation, clasmatosis, screenings, obtain being rich in the pumpkin seeds essence of anti-hyperplasia factor citrulling and lipid.
2. the method for claim 1 is characterized in that concrete steps are:
(1) the fresh pumpkin seeds benevolence of peeling off of screening is rejected flat grain, broken lobe grain, the grain of damaging by worms, and is clean through the clear water rinsing, places 75～95 ℃ of hot-water soak grooves to soak then and scald 1～10min, lipoxidase activity in the passivation pumpkin seeds benevolence blastocyte;
(2) adopt common cell breaking plant with the fragmentation of pumpkin seeds benevolence, the same category of device that also can adopt grinding mill, colloid mill, bruisher or have the smudge cells function of milling; Add entry before and after the clasmatosis, addition is 0.5～3 times of pumpkin seeds benevolence weight, pumpkin seeds benevolence screenings that again will be through pulverizing is through common screenings separator or centrifuge, filter separating and filtering, rotating speed 3000～20000r/min, the pumpkin seeds benevolence emulsion of anti-hyperplasia factor citrulling and lipid is rich in acquisition;
(3) allotment emulsification homogeneous adopts emulsification of vacuum emulsification equipment or Homogenizing pump homogeneous, pressure 200～1000kg/cm usually
2, speed of agitator 1000～20000r/min can also select to add emulsifying agent, flavor enhancement, thickener according to the needs of design before allocating the emulsification homogeneous or behind the emulsification homogeneous; But spray-drying working pressure spray-drying or centrifugal spray drying, pressure 100～600kg/em
2, centrifugal rotational speed 1000～30000r/min, the hot-wind inlet temperature is 160～190 ℃, outlet temperature is 70～85 ℃; Also can adopt common industrial vacuum freeze drying plant to carry out drying.
3. method as claimed in claim 2 is characterized in that selecting in the concrete steps (3) to add emulsifying agent, flavor enhancement, and thickener is respectively:
(1) according to the needs that design, emulsifying agent can select following one or more: single palmitic acid sorb glucosides ester, Emulsifier LT-60M, xylitan monostearate, glycerol monostearate acid, tripalmitin, cephalin, lipositol, glycophospholipin, lecithin, sorbitan mono-oleic acid ester, sorbitol anhydride tristearate, sorbitan monostearate, sucrose fatty ester, hydrogenated rosin glyceride, propylene glycol ester, the consumption of emulsifying agent is 0.000004～0.1% of a pumpkin seeds benevolence weight;
(2) flavor enhancement can select following one or more: sodium chloride, potassium chloride, sucrose, fructose, fruit glucose, glucose, maltose, compound sugar, xylo-oligosaccharide, oligoisomaltose, beet sugar, maltitol, isomerizing lactose, stevia, Aspartame, albumen sugar, acetic acid, citric acid, malic acid, tartaric acid, sodium phosphate, the consumption of flavor enhancement is 0.00005～20% of a pumpkin seeds benevolence weight;
(3) thickener can select following one or more: methylcellulose, CMC, ethyl cellulose, HYDROXY PROPYL METHYLCELLULOSE and microcrystalline cellulose, starch, converted starch, dextrin, maltodextrin, Sodium Hydroxymethyl Stalcs, edible gelatin, Sodium Caseinate, vegetable protein, animal protein, Arabic gum, sesbania gum, pectin, agar, locust bean gum, prestige Luo Zi glue, xanthan gum, marine alga propylene glycol ester, alginates, carragheen, guar gum, bassora gum, the consumption of thickener are 0.00004～44% of pumpkin seeds benevolence weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03126659A CN1452915A (en) | 2003-05-26 | 2003-05-26 | Prepn of pumpkin seed essence with rich citrulline and lipoid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03126659A CN1452915A (en) | 2003-05-26 | 2003-05-26 | Prepn of pumpkin seed essence with rich citrulline and lipoid |
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| CN1452915A true CN1452915A (en) | 2003-11-05 |
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| CN03126659A Pending CN1452915A (en) | 2003-05-26 | 2003-05-26 | Prepn of pumpkin seed essence with rich citrulline and lipoid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102551119A (en) * | 2010-12-16 | 2012-07-11 | 苏州工业园区尚融科技有限公司 | Production method of sugar-free healthy sunflower seeds |
| CN104664031A (en) * | 2015-02-05 | 2015-06-03 | 浙江小二黑食品有限公司 | Novel ice cream with corn and preparation method thereof |
-
2003
- 2003-05-26 CN CN03126659A patent/CN1452915A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102551119A (en) * | 2010-12-16 | 2012-07-11 | 苏州工业园区尚融科技有限公司 | Production method of sugar-free healthy sunflower seeds |
| CN104664031A (en) * | 2015-02-05 | 2015-06-03 | 浙江小二黑食品有限公司 | Novel ice cream with corn and preparation method thereof |
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