CN1439710A - Environment protecting bacteria for stool fermentation and its preparation - Google Patents
Environment protecting bacteria for stool fermentation and its preparation Download PDFInfo
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- CN1439710A CN1439710A CN03109680A CN03109680A CN1439710A CN 1439710 A CN1439710 A CN 1439710A CN 03109680 A CN03109680 A CN 03109680A CN 03109680 A CN03109680 A CN 03109680A CN 1439710 A CN1439710 A CN 1439710A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
A microbial preparation for fermenting excrement and urine is prepared from vididin, high-temp bacillus, lactic acid bacteria and actinomycetes through three class culturing and proportional mixing. Its advantages are both fermenting and deodouring functions, and low cost.
Description
Technical field
The present invention relates to manure fermentation microbiobacterial agent of a kind of environment-friendly type and preparation method thereof, belong to microbial fermentation technology.
Background technology
At present, be applied to the existing multiple patent of microbial fermentation microbial inoculum and the bibliographical information of feces of livestock and poultry fermentative processing, they roughly can be divided into three types: a kind of is composting process, promptly under aerobic condition, adopt the microbial reproduction of refuse itself, make refuse ferment, the shortcoming of this method is that fermentation time is long, brings certain influence to environment in the fermenting process; Second method is the fermentation that some microbiobacterial agents with fermentation function is applied to ight soil, the advantage of this method is the fermentation time weak point, generally can controls, yet topmost deficiency is not make full use of the thermophilic fermentation microbial inoculum, often fermentation is not thorough, and the stink in the ight soil can not get effectively control and handles simultaneously; The third method is environmental friendliness efficacious microbial colony (being also referred to as the EM flora), belongs to more than 80 kind of microorganism by 10 of mould, milk-acid bacteria, yeast, actinomycetes and photosynthetic bacteriums etc. and forms.This flora has the multi-functional of fermentation, deodorizing, effective kill pathogens, the environmentally advantageous fermentative microflora of generally acknowledging at present.Yet because flora is huge, cost is very high, and application is restricted.The present invention adopt from soil or ight soil isolating in warm cellulose-decomposing bacterium, high temperature cellulose-decomposting bacteria, effectively combine deodorizing microorganism simultaneously, formed the better simply microbial inoculum of only forming by four kinds of bacterial strains.
Summary of the invention
The object of the present invention is to provide manure fermentation microbiobacterial agent of a kind of environment-friendly type and preparation method thereof, this microbial inoculum is fast for manure fermentation speed, environmentally safe; Described preparation method's process is simple, cost is low.
The present invention is realized by following technical proposals.Obtain through separating and cultivating: every gram contains 2.5 * 10
8-3.2 * 10
8Number viride (Trichoderma viride), every gram contain 1.3 * 10
8-1.7 * 10
8The high temperature genus bacillus of number (Bacillus sp.), every gram contain 1.2 * 10
8-1.6 * 10
8Number milk-acid bacteria (Lactococcus lactis) and every gram contain 1.2 * 10
8-1.8 * 10
8The actinomycetes of number (Streptomyces).Then, with they etc. quality mix and just become the microbial fermentation microbial inoculum.Wherein, viride, high temperature genus bacillus, milk-acid bacteria and actinomycetes each bacterium bacterium number in every gram microbial inoculum is than being 38-42: 17-23: 16-21: 15-23.
The preparation method of above-mentioned microbial inoculum comprises the cultivation of various microbial inoculums and their mixing, and its concrete steps are as follows:
1, the cultivation of viride
Viride at first on the PDA substratum, 30 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 3 * 10
8Individual/the gram solid medium;
2, the cultivation of high temperature genus bacillus
The high temperature genus bacillus at first on the meat soup protein culture medium, 30 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.5 * 10
8Individual/the gram solid medium;
3, the cultivation of milk-acid bacteria
Milk-acid bacteria on malt extract medium, 28 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.5 * 10
8Individual/the gram solid medium;
4, actinomycetic cultivation
Actinomycetes at first on the Gause I substratum, 28 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.5 * 10
8Individual/the gram solid medium.
5, the mixing of microbial inoculum
With 1,2,3,4 four kinds of quality such as microbial inoculum solid medium mixing of being cultivated set by step, just obtained the organism of fermentation microbial inoculum.
The present invention compared with prior art has following advantage: this microbial inoculum will ferment and two kinds of functions of deodorizing organically combine, and alleviate labour intensity greatly, effectively prevent that nutritive element ground runs off simultaneously again.Simultaneously, the decomposition of thermophase had taken into account the decomposition of hot stage simultaneously again during this microbial inoculum laid particular emphasis on, and had strengthened ferment effect greatly.Its preparation method process is simple, and cost is low, and applicability is strong, especially is fit to medium-sized and small enterprises and uses.
Embodiment
Embodiment 1
Viride, high temperature genus bacillus, milk-acid bacteria and actinomycetes are independently cultivated respectively, made the viride number in every gram, reach 3 * 10
8, high temperature genus bacillus number reaches 1.5 * 10 in every gram
8, the milk-acid bacteria number reaches 1.5 * 10 in every gram
8, the actinomycetes number reaches 1.5 * 10 in every gram
8, then quality such as each microbial inoculum solution are mixed, make viride in every gram mix bacterium agent, high temperature genus bacillus, milk-acid bacteria and actinomycetes number ratio reach 40: 20: 20: 20.After placing fermenter to carry out pre-treatment the feces of livestock and poultry, under 25 ℃-30 ℃, add 5% high-effective microorganism fermenting agent, stir, and aeration regularly, can reach fully in 15 days and to become thoroughly decomposed, the material that becomes thoroughly decomposed is grey and the smell that gives out wine brewing.
Embodiment 2
10 kilograms of freshly-slaughtered poultry excrement are through after the pre-treatment, the adding weight ratio is 5% high-effective microorganism fermenting agent, moisture content is adjusted to 45%, began to ferment 10 days in 28 ℃, 50 ℃-55 ℃ fermentations 2 days, stir while fermenting, obtain the leavened prod that grey has the wine brewing taste after fermentation is finished, simultaneously in contrast with the spontaneous fermentation thing.The result shows (as table 1): as can be seen, the chicken manure that adds the fermenting agent secondary fermentation is obvious strong than spontaneous fermentation to the crystallized ability of organic matter, even can exceed 42% from the table, and ammonia nitrogen content is lower.We know that ammonia-state nitrogen is the major cause that unpleasant odor produces, and stink obviously reduces in the chicken manure fermenting process behind the adding fermenting agent, and this is with theoretical consistent.The nitrate nitrogen content that adds bacterium fermentation back material is more than 3 times of spontaneous fermentation, can be very strong to the crystallized ability of nutritive element.Phosphorus and potassium content also slightly exceed a bit.
The main physical and chemical index of table 1 chicken manure fermenting
| Project | Not fermentation | Add the bacterium fermentation | Spontaneous fermentation (contrast) | ||
| Moisture content (%) | ??45.00 | ??48.49 | ??50.26 | ??56.08 | ??62.42 |
| Organic (%) | ??48.34 | ??43.14 | ??40.75 | ??37.01 | ??28.55 |
| Full nitrogen (%) | ??2.50 | ??2.57 | ??2.39 | ??1.94 | ??1.25 |
| Ammonia nitrogen (%) | ??0.14 | ??0.26 | ??0.28 | ??0.41 | ??0.58 |
| Nitric nitrogen (mg/kg) | ??- | ??350.84 | ??301.72 | ??112.56 | ??113.54 |
| Phosphorus (%) | ??0.55 | ??0.54 | ??0.47 | ??0.43 | ??0.38 |
| Potassium (%) | ??1.20 | ??1.22 | ??1.22 | ??1.12 | ??1.00 |
Claims (2)
1, a kind of manure fermentation microbiobacterial agent of environment-friendly type is characterized in that: will obtain through separating and cultivating: every gram contains 3 * 10
8Number viride, every gram contain 1.5 * 10
8Number high temperature genus bacillus, every gram contain 1.5 * 10
8Number milk-acid bacteria and every gram contain 1.5 * 10
8The number actinomycetes, then, according to viride, high temperature genus bacillus, milk-acid bacteria and actinomycetes respectively with each the bacterium bacterium number in every gram microbial inoculum than 38-42: 17-23: 16-21: 15-23 mixes.
2, press the preparation method of the manure fermentation microbiobacterial agent of the described environment-friendly type of claim 1, this method comprises the cultivation and the mixing of microbial inoculum, it is characterized in that step is as follows:
1), the cultivation of viride
Viride at first on the PDA substratum, 30 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 3 * 10
8Individual/the gram solid medium;
2), the cultivation of high temperature genus bacillus
The high temperature genus bacillus at first on the meat soup protein culture medium, 30 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.5 * 10
8Individual/the gram solid medium;
3), the cultivation of milk-acid bacteria
Milk-acid bacteria on malt extract medium, 28 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.5 * 10
8Individual/the gram solid medium;
4), actinomycetic cultivation
Actinomycetes at first on the Gause I substratum, 28 ℃, make primary inclined plane and cultivate, be inoculated into then and make vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.5 * 10
8Individual/the gram solid medium;
5), the mixing of microbial inoculum
Will be set by step 1), 2), 3), 4) four kinds of microbial inoculum solid mediums cultivating at room temperature wait quality to mix, and have just obtained the organism of fermentation microbial inoculum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03109680A CN1439710A (en) | 2003-04-11 | 2003-04-11 | Environment protecting bacteria for stool fermentation and its preparation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN03109680A CN1439710A (en) | 2003-04-11 | 2003-04-11 | Environment protecting bacteria for stool fermentation and its preparation |
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| Publication Number | Publication Date |
|---|---|
| CN1439710A true CN1439710A (en) | 2003-09-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN03109680A Pending CN1439710A (en) | 2003-04-11 | 2003-04-11 | Environment protecting bacteria for stool fermentation and its preparation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425690C (en) * | 2006-07-13 | 2008-10-15 | 中国农业科学院农业环境与可持续发展研究所 | Fast harmless biological treating process of animal excrement and manure water |
| CN102584370A (en) * | 2012-02-29 | 2012-07-18 | 王宝申 | Technology for preparing bio-fertilizer from garbage |
| CN104744091A (en) * | 2015-03-19 | 2015-07-01 | 陕西沁稼生态科技有限公司 | Method for preparing organic material decomposing microbial inoculum based on white trichoderma and bacillus subtilis |
| CN112501030A (en) * | 2020-11-30 | 2021-03-16 | 九江礼涞生物科技有限公司 | Application of livestock and poultry manure leavening agent product |
| CN112812990A (en) * | 2020-12-31 | 2021-05-18 | 宜兴艾科森生态环卫设备有限公司 | Compound microbial agent for excrement treatment and application and compounding method thereof |
-
2003
- 2003-04-11 CN CN03109680A patent/CN1439710A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100425690C (en) * | 2006-07-13 | 2008-10-15 | 中国农业科学院农业环境与可持续发展研究所 | Fast harmless biological treating process of animal excrement and manure water |
| CN102584370A (en) * | 2012-02-29 | 2012-07-18 | 王宝申 | Technology for preparing bio-fertilizer from garbage |
| CN104744091A (en) * | 2015-03-19 | 2015-07-01 | 陕西沁稼生态科技有限公司 | Method for preparing organic material decomposing microbial inoculum based on white trichoderma and bacillus subtilis |
| CN112501030A (en) * | 2020-11-30 | 2021-03-16 | 九江礼涞生物科技有限公司 | Application of livestock and poultry manure leavening agent product |
| CN112812990A (en) * | 2020-12-31 | 2021-05-18 | 宜兴艾科森生态环卫设备有限公司 | Compound microbial agent for excrement treatment and application and compounding method thereof |
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