CN1435185A - Cell growth regulatory factor A and preparing process thereof - Google Patents

Abstract

A cell growth regulatory factor A for improving immunity and treating cervical or lumbar intervertebral disk protrusion is prepared through screening gram-positive and gram-negative cocci or bacillis, freeze storage, culturing at 4-38 deg.C, filtering for removing harmful bacteria, extracting natural gene, quantitatively adding ornithin, safety examining, and encapsulating.

Description

Cell growth regulatory factor A and preparation method

Technical field

What the present invention relates to is a kind of medicine and preparation method that is of value to the Growth of Biologic Cell regulation and control, belongs to a kind of biological medicine.

Background technology

The mankind, birth and old age, sickness and death are a kind of natural laws, along with wearing out of human body cell, the regeneration function of its cell will be lost gradually, human body enters aging period, and the intravital various cellular degenerations of people are had an operation as the old man and to be recovered to want the slow well evidence that is mostly more than the adolescent.The aging of people mainly shows on the cell senescence and deterioration of various internal organs, thereby in recent years, the medical expert of countries in the world is attempting to study relevant " the long-lived medicine " that increases man's lifespan.So-called long-lived medicine is exactly nothing but that this medicine can promote functions such as the enhancing of the regeneration of human body cell and repair ability and improvement.Yet the file that yet there are no similar contents such as " long-lived medicines " is published.On the other hand, even if the someone does not also enter old age, but still will there be its situation about can not normally repair of necrosis of human cell tissue in the part of its human body, as cervical spondylosis patient or prolapse of lumbar intervertebral disc patient or the like.These patients are in the majority with the older, degeneration, atrophy, downright bad cell fibrosis tissue existence mainly still because taken place in the mechanism of its morbidity in the part of its human body, conventional treatment method is exactly an excision, again plantation tissue or transplanting are corresponding then organizes, this therapeutic method of surgery is passive, and do not have the positive therapeutic effect, and it can only be treated devastatingly, and its effect is not desirable.Also have a kind of collagenase or collagen protein of using to treat, but collagenase and collagen protein only have dissolution to the fibrosis tissue of necrosis, but do not have the pair cell tissue regeneration to be arranged, effect such as repair and mould again again, thereby in fact they do not have any good therapeutic effect.

Summary of the invention

The object of the present invention is to provide and a kind of human body cell is had the adjusting and controlling growth effect, strengthen the n cell growth regulatory factor A and the preparation method of functions such as the regeneration of inside of human body cell tissue, reparation, contain natural gene, the ornithine that extracts in Gram-positive, negative cocci or the bacillus among this n cell growth regulatory factor A, and the configurable ornithine that has more than the 1 μ g of 1 μ g gene.

1 μ g gene disposes the ornithine of 1 μ g-500mg among the described n cell growth regulatory factor A.

1 μ g gene disposes the ornithine of 1-200mg among the described n cell growth regulatory factor A.

A kind of method of producing n cell growth regulatory factor A, it is earlier Gram-positive, negative cocci or bacillus to be screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 4-38 ℃ of temperature, and after at least once removing by filter harmful bacteria, stay useful bacterial chip and promptly extract natural gene, in natural gene, quantitatively add ornithine, after safety detection, water preparation closes to seal and forms under the GMP standard.

Described bacterial strain is conventional the cultivation under 10-35 ℃ of temperature, and stays useful bacterial chip promptly extract natural gene after secondary filter is removed harmful bacteria at least.

The present invention is by screening Gram-positive, negative cocci or bacillus, through cultivating, filter removal impurity and harmful substance, as the thalline that causes a disease, useful bacterial chip---the natural gene that extraction stays, so-called natural gene is meant that filtrate such as useful bacterial chip do not change its molecular structure and chemical physical property, thereby be natural, institute's tht gene is meant that useful bacterial chip is a kind of little thalline measurement unit; This useful gene pairs human body cell has the adjusting and controlling growth effect, groups of cells to necrosis is woven with reparation and shaping action more again, after itself and ornithine be mixed and made into n cell growth regulatory factor A, both have synergism, its effect will be more obvious, have and better impel body to activate raise immunity, TC is strengthened, and make the aging of cervical vertebra lumbar intervertebral disc, downright bad, degeneration, atrophy, Fibrotic histiocyte is repaired again, mould again, blood capillary regeneration, grow again, recover one's youthful vigour thereby play intervertebral disc, slow down aging, thus make multiple neck, prolapse of lumbar intervertebral disc, degeneration is repaired again, the effect of moulding again.Described useful bacterial chip, its component comprise the genetic stew of thalline, the secretions of thalline and the culture medium complex of this thalline.

The trade name of n cell growth regulatory factor A of the present invention is spirit in Huan days, and it treats multiple neck, prolapse of lumbar intervertebral disc compressing N; Through the animal safety test, acute subacute toxicity test feminine gender there is no undue toxicity's side effect, the long term toxicity test feminine gender; Clinical efficacy can make blood capillary proliferation propagation between the tissue of degeneration of intervertebral disc, moulds thereby make to organize to repair again again.Model case:

Case 1, the week * *, the male, 52 years old, multiple intervertebral disc of cervical vertebra compressing spinal cord was 5 years before the medication, and the compressing spinal cord is more than 1/2.Follow up a case by regular visits to intervertebral disc reparation, spinal cord regeneration with a year and a half after the n cell growth regulatory factor A Drug therapy of the present invention; The week * *, before the medication, the multiple intervertebral disk hernia in MRIC5-6 transverse section is the Semen Glycines size, and spinal cord flattens; The spinal cord that the dissolving of MRI transverse section cervical vertebra 5-6 intervertebral disc fragment absorbs, flattens after the medication is long again multiple.

Case 2, woods * *, the woman, 59 years old, it is big that the preceding multiple especially C6-7 intervertebral disc compressing spinal cord of medication is Semen Glycines, and spinal cord is pressed 2/3.With behind the medicine of the present invention 1 year, the C6-7 intervertebral disk hernia dissolved, clinical symptoms disappears.C6-7 intervertebral disk hernia compressing spinal cord nearly 1/2 before the medication.MRI transverse section C6-7 intervertebral disk hernia district dissolving after the medication, spinal cord is long multiple.

Case 3, Chen Hangjiang, the Malaysian, prolapse of lumbar intervertebral disc, pressuring nerve can not be walked, and can not go up downstairs, and in 6 weeks after this product treatment, resolution of symptoms can normally be walked, former outstanding molten Jie of intervertebral of MRI.

The specific embodiment

The present invention will be described in detail below in conjunction with embodiment:

Embodiment 1, earlier gram-positive cocci is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 6 ℃ of temperature, the general low more incubation time of temperature is long more, bacterial strain stays useful bacterial chip after successively removing by filter impurity and harmful bacteria four times after fully cultivating, be the extraction natural gene, because the gram coccus is a bacterial strain in spite of illness common in the clinical medicine, the public can obtain from public occasion, the sample for test that is provided in the laboratory as various big hospital, therefore this strain need not special preservation, and it through after cultivating fully and filtering, can be reached the purpose of removing disease carrying germ.Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 1mg ornithine therein, measures content and after safety detection, and water preparation or freezing dry powder close envelope and form under the GMP standard, after product inspection is qualified, and printing package.Described urinary ammonia acid is conventional amino acid whose a kind of, and the public can obtain from public occasion easily.

Embodiment 2, earlier Gram-negative coccus is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 12 ℃ of temperature, bacterial strain stays useful bacterial chip after successively removing by filter impurity and harmful bacteria three times after fully cultivating, be the extraction natural gene; Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 3mg ornithine therein, measures content and after safety detection, water preparation or freezing dry powder close envelope and form n cell growth regulatory factor A under the GMP standard, after product inspection is qualified, and printing package.

Embodiment 3, earlier gram negative bacilli is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 20 ℃ of temperature, bacterial strain stays useful bacterial chip after removing impurity and harmful bacteria through the priority secondary filter after the abundant cultivation, be the extraction natural gene; Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 8mg ornithine therein, measures content and after safety detection, water preparation or freezing dry powder close envelope and form n cell growth regulatory factor A under the GMP standard, after product inspection is qualified, and printing package.

Embodiment 4, earlier Gram-positive bacillus is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 38 ℃ of temperature, bacterial strain stays useful bacterial chip through after once removing by filter impurity and harmful bacteria after fully cultivating, be the extraction natural gene, it is many more normally to filter number of times, and the useful bacterial chip of left (extraction) is that natural gene is just pure more.Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 20mg ornithine therein, measures content and after safety detection, water preparation or freezing dry powder close envelope and form n cell growth regulatory factor A under the GMP standard, after product inspection is qualified, and printing package.

Other embodiments of the invention all can dispose the interior arbitrarily configuration of scope of 1 μ g-500mg ornithine at gene 1 μ g.

Claims (5)

1, a kind of n cell growth regulatory factor A, it is characterized in that containing natural gene, the ornithine that extracts in Gram-positive, negative cocci or the bacillus among this n cell growth regulatory factor A, wherein the configurable ornithine that has more than the 1 μ g of 1 μ g natural gene.

2, n cell growth regulatory factor A according to claim 1 is characterized in that among the described n cell growth regulatory factor A, 1 μ g natural gene disposes the ornithine of 1 μ g-500mg.

3, n cell growth regulatory factor A according to claim 2 is characterized in that among the described n cell growth regulatory factor A, 1 μ g natural gene disposes the ornithine of 1-200mg.

4, a kind of method of producing as claim 1 or 2 or 3 described n cell growth regulatory factor A, it is earlier Gram-positive, negative cocci or bacillus to be screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 4-38 ℃ of temperature, and after at least once removing by filter harmful bacteria, stay useful bacterial chip and promptly extract natural gene, in natural gene, quantitatively add ornithine, after safety detection, water preparation closes to seal and forms under the GMP standard.

5, the method for n cell growth regulatory factor A according to claim 4 is characterized in that described bacterial strain conventional cultivation under 10-35 ℃ of temperature, and stays useful bacterial chip promptly extract gene after secondary filter is removed harmful bacteria at least.