CN1305465C - Cell growth regulating factor I and its prepn process - Google Patents
Cell growth regulating factor I and its prepn process Download PDFInfo
- Publication number
- CN1305465C CN1305465C CNB021510962A CN02151096A CN1305465C CN 1305465 C CN1305465 C CN 1305465C CN B021510962 A CNB021510962 A CN B021510962A CN 02151096 A CN02151096 A CN 02151096A CN 1305465 C CN1305465 C CN 1305465C
- Authority
- CN
- China
- Prior art keywords
- cell growth
- alanine
- growth regulating
- natural
- natural gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to natural cell growth regulating factor I and a preparation method thereof. The preparation method comprises the following steps: firstly, sieving Gram-positive cocci and Gram-negative cocci or bacilli, and freezing and preserving a strain; conventionally cultivating the strain at 4 to 38 DEG C and leaving beneficial thallus fragments via filtration of at least one time to remove harmful thalli, namely extracting natural genes; adding quantitive alanine in the natural genes; after safety test, sealing a water agent under a GMP standard to prepare the natural cell growth regulating factor I. 1 mu g of the natural genes of the prepared natural cell growth regulating factor I can be compounded with alanine of more than 1 mu g. The natural cell growth regulating factor I can better prompt a body to activate and reinforce immunological functions so as to reinforce TC, re-repair and reshape senescent, necrotic, denatured, atrophic and fiberized pancreatic tissue cells and regenerate and replant capillary vessels to perform the functions of rejuvenescence and senescence delay.
Description
Technical field
What the present invention relates to is a kind of medicine and preparation method that is of value to the Growth of Biologic Cell regulation and control, belongs to a kind of biological medicine.
Background technology
The mankind, birth and old age, sickness and death are a kind of natural laws, along with wearing out of human body cell, the regeneration function of its cell will be lost gradually, human body enters aging period, and the intravital various cellular degenerations of people are had an operation as the old man and to be recovered to want the slow well evidence that is mostly more than the adolescent.The aging of people mainly shows on the cell senescence and deterioration of various internal organs, thereby in recent years, the medical expert of countries in the world is attempting to study relevant " the long-lived medicine " that increases man's lifespan.So-called long-lived medicine is exactly nothing but that this medicine can promote functions such as the enhancing of the regeneration of human body cell and repair ability and improvement.Yet the file that yet there are no similar contents such as " long-lived medicines " is published.On the other hand, even if the someone does not also enter old age, but still will there be its situation about can not normally repair of necrosis of human cell tissue in the part of its human body, as cervical spondylosis patient or prolapse of lumbar intervertebral disc patient or the like.These patients are in the majority with the older, degeneration, atrophy, downright bad cell fibrosis tissue existence mainly still because taken place in the mechanism of its morbidity in the part of its human body, conventional treatment method is exactly an excision, again plantation tissue or transplanting are corresponding then organizes, this therapeutic method of surgery is passive, and do not have the positive therapeutic effect, and it can only be treated devastatingly, and its effect is not desirable.Also have a kind of collagenase, matter acid enzyme or collagen protein of using to treat, but collagenase, matter acid enzyme and collagen protein only have dissolution to the fibrosis tissue of necrosis, but there is not the pair cell tissue regeneration to be arranged, effect such as repair and mould again again, thereby in fact they do not have any good therapeutic effect.
Summary of the invention
The object of the present invention is to provide and a kind of human body cell is had the adjusting and controlling growth effect, strengthen the n cell growth regulatory factor I and the preparation method of functions such as the regeneration of inside of human body cell tissue, reparation, contain natural gene, the alanine that extracts in Gram-positive, negative cocci or the bacillus among this n cell growth regulatory factor I, and the configurable alanine that has more than the 1 μ g of 1 μ g natural gene.
1 μ g natural gene disposes 1 μ g---the alanine of 500mg among the described n cell growth regulatory factor I.
1 μ g natural gene disposes 1 among the described n cell growth regulatory factor I---the alanine of 200mg.
A kind of method of producing n cell growth regulatory factor I, it is earlier Gram-positive, negative cocci or bacillus to be screened and the freezing bacterial strain, with bacterial strain 4---the conventional cultivation under 38 ℃ of temperature, and after at least once removing by filter harmful bacteria, stay useful bacterial chip and promptly extract natural gene, in natural gene, quantitatively add alanine, after safety detection, water preparation or freezing dry powder close to seal and form under the GMP standard.Described useful bacterial chip, its component comprise the genetic stew of thalline, the secretions of thalline and the culture medium complex of this thalline.
Described bacterial strain is 10---the conventional cultivation under 35 ℃ of temperature, and after secondary filter is removed harmful bacteria at least, stay useful bacterial chip and promptly extract natural gene.
The present invention is by screening Gram-positive, negative cocci or bacillus, through cultivating, filter removal impurity and harmful substance, as the thalline that causes a disease, the useful bacterial chip that extraction stays is a natural gene, so-called natural gene is meant that filtrate such as useful bacterial chip do not change its molecular structure and chemical physical property, thereby be natural, institute's tht gene is meant that useful bacterial chip is a kind of measurement unit of somatic cells; This useful gene pairs human body cell has the adjusting and controlling growth effect, groups of cells to necrosis is woven with reparation and shaping action more again, after itself and alanine be mixed and made into n cell growth regulatory factor I, both have synergism, its effect will be more obvious, have and better impel body to activate raise immunity, TC is strengthened, and aging, necrosis, degeneration, atrophy, Fibrotic pancreatic tissue cell are repaired again, mould again, blood capillary is regenerated, is grown, and intervertebral disc is recovered one's youthful vigour, function in delaying senility thereby play.
N cell growth regulatory factor I of the present invention, its commodity day pancreas spring by name, specially for treating diabetes I, II phase; Through animal safety test, acute subacute toxicity test feminine gender, undue toxicity, long term toxicity test feminine gender.
Model case:
Case 1, Wang XX, the women, 70 years old, diabetes II use insulin every day phase, through the made medicine of the present invention---day pancreas spring treat after 3 weeks, be clearly better, it is controlled to treat after 3 months the state of an illness.
Case 2, pottery XX, male, 56 years old, diabetes II phase, the senile decline of companion's first; Be clearly better after 3 weeks of the course of treatment of treatment through this medicine, glucose in urine and blood glucose obviously descend, among further treating.
Case 3, money XX, male, 68 years old, diabetes II, III phase; After the treatment of this medicine, be clearly better.
The specific embodiment
The present invention will be described in detail below in conjunction with embodiment:
Embodiment 1, earlier gram-positive cocci is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 6 ℃ of temperature, the general low more incubation time of temperature is long more, bacterial strain stays useful bacterial chip after successively removing by filter impurity and harmful bacteria four times after fully cultivating, be the extraction natural gene, because the gram coccus is a bacterial strain in spite of illness common in the clinical medicine, therefore this strain need not special preservation, it through after cultivating fully and filtering, can be reached the purpose of removing disease carrying germ.Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 1mg alanine therein, measures content and after safety detection, and water preparation closes envelope and forms under the GMP standard, after product inspection is qualified, and printing package.
Embodiment 2, earlier Gram-negative coccus is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 12 ℃ of temperature, bacterial strain stays useful bacterial chip after successively removing by filter impurity and harmful bacteria three times after fully cultivating, be the extraction natural gene; Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 3mg alanine therein, measures content and after safety detection, water preparation closes envelope and forms n cell growth regulatory factor I under the GMP standard, after product inspection is qualified, and printing package.
Embodiment 3, earlier gram negative bacilli is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 20 ℃ of temperature, bacterial strain stays useful bacterial chip after removing impurity and harmful bacteria through the priority secondary filter after the abundant cultivation, be the extraction natural gene; Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 8mg alanine therein, measures content and after safety detection, water preparation or freezing dry powder close envelope and form n cell growth regulatory factor I under the GMP standard, after product inspection is qualified, and printing package.
Embodiment 4, earlier Gram-positive bacillus is screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 38 ℃ of temperature, bacterial strain stays useful bacterial chip through after once removing by filter impurity and harmful bacteria after fully cultivating, be the extraction natural gene, it is many more normally to filter number of times, and the useful bacterial chip of left (extraction) is that natural gene is just pure more.Getting useful bacterial chip is natural gene 1 μ g, quantitatively adds the 20mg alanine therein: measure content and after safety detection, water preparation closes envelope and forms n cell growth regulatory factor I under the GMP standard, and after product inspection is qualified, printing package.
Other embodiments of the invention all can dispose 1 μ g at gene 1 μ g---arbitrarily configuration in the scope of 500mg alanine.
Claims (4)
1, a kind of n cell growth regulatory factor I, it is characterized in that: contain in Gram-positive, negative cocci or the bacillus among this n cell growth regulatory factor I and extract natural gene, also contain alanine, wherein 1 μ g natural gene disposes the alanine of 1 μ g-500mg.
2, n cell growth regulatory factor I according to claim 1 is characterized in that: among the described n cell growth regulatory factor I, 1 μ g natural gene disposes the alanine of 1-200mg.
3, the preparation method of n cell growth regulating device control factor I as claimed in claim 1 or 2, it is characterized in that: it is earlier Gram-positive, negative cocci or bacillus to be screened and the freezing bacterial strain, with bacterial strain conventional cultivation under 4-38 ℃ of temperature, and after at least once removing by filter harmful bacteria, stay useful bacterial chip and promptly extract natural gene, in natural gene, quantitatively add alanine, after safety detection, water preparation or freezing dry powder close to seal and form under the GMP standard.
4, the preparation method of n cell growth regulatory factor I according to claim 3 is characterized in that: described bacterial strain is conventional the cultivation under 10-35 ℃ of temperature, and stays useful bacterial chip promptly extract gene after secondary filter is removed harmful bacteria at least.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB021510962A CN1305465C (en) | 2002-12-06 | 2002-12-06 | Cell growth regulating factor I and its prepn process |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB021510962A CN1305465C (en) | 2002-12-06 | 2002-12-06 | Cell growth regulating factor I and its prepn process |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1506060A CN1506060A (en) | 2004-06-23 |
CN1305465C true CN1305465C (en) | 2007-03-21 |
Family
ID=34234253
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB021510962A Expired - Fee Related CN1305465C (en) | 2002-12-06 | 2002-12-06 | Cell growth regulating factor I and its prepn process |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1305465C (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1044468A (en) * | 1989-01-28 | 1990-08-08 | 杭州市第三人民医院 | Cell growth stimulant and manufacture method thereof |
-
2002
- 2002-12-06 CN CNB021510962A patent/CN1305465C/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1044468A (en) * | 1989-01-28 | 1990-08-08 | 杭州市第三人民医院 | Cell growth stimulant and manufacture method thereof |
Also Published As
Publication number | Publication date |
---|---|
CN1506060A (en) | 2004-06-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11884953B2 (en) | Method for preparing protein peptide based on connective tissue and prepared protein peptide and use thereof | |
CN104163847B (en) | The preparation method of fly maggot active protein peptide and prepared fly maggot active protein peptide and application thereof | |
CN1305465C (en) | Cell growth regulating factor I and its prepn process | |
CN1263459C (en) | Cell growth regulating factor O and its prepn process | |
CN1263460C (en) | Cell growth regulating factor G and its prepn process | |
CN1241575C (en) | Cell growth regulating factor U and its prepn process | |
CN1323670C (en) | Cell growth regulatory factor L and preparing process thereof | |
CN1235594C (en) | Cell growth regulating factor Q and its prepn process | |
CN1263461C (en) | Cell growth regulatnig factor F and its prepn process | |
CN1233328C (en) | Cell growth regulating factor K and its prepn process | |
CN1323669C (en) | Cell growth regulating factor P and its prepn process | |
CN1235593C (en) | Regulatary factor T for cell growth, and its prepn. method | |
CN1323671C (en) | Cell growth regulating factor J and its prepn process | |
CN1255112C (en) | Cell growth regulating factor E and its prepn process | |
CN1506052A (en) | Cell growth regulating factor X and its prepn process | |
CN1293866C (en) | Cell growth regulating factor II and its prepn process | |
CN1255113C (en) | Cell growth regulating factor D and its prepn process | |
CN1323672C (en) | Cell growth regulatory factor C and preparing process thereof | |
CN1293875C (en) | Cell growth regulatnig factor S and its prepn process | |
CN1293865C (en) | Cell growth regulating factor R and its prepn process | |
CN1435185A (en) | Cell growth regulatory factor A and preparing process thereof | |
CN1421211A (en) | Cell growth regulating factor M and its prepn process | |
CN1506075A (en) | Cell growth regulating factor V and its prepn process | |
CN1245164C (en) | Cell growth regulating factor oral liquid and its prepn process | |
CN106036852A (en) | Method for preparing probiotic fermented functional healthcare food with arctium lappa |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
PP01 | Preservation of patent right |
Effective date of registration: 20171101 Granted publication date: 20070321 |
|
PP01 | Preservation of patent right | ||
PD01 | Discharge of preservation of patent |
Date of cancellation: 20200122 Granted publication date: 20070321 |
|
PD01 | Discharge of preservation of patent | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20070321 Termination date: 20201206 |
|
CF01 | Termination of patent right due to non-payment of annual fee |