CN1435087A - Method for artificial fertilization and hatching of kanglang whitefish - Google Patents
Method for artificial fertilization and hatching of kanglang whitefish Download PDFInfo
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- CN1435087A CN1435087A CN02113288A CN02113288A CN1435087A CN 1435087 A CN1435087 A CN 1435087A CN 02113288 A CN02113288 A CN 02113288A CN 02113288 A CN02113288 A CN 02113288A CN 1435087 A CN1435087 A CN 1435087A
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- hatching
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- fertilization
- angler
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
An artificial fertilization and hatching method for Anabarilius grahami includes slightly squeezing out the ova and spermia from the sexually mature female and male Anabarilius grahami, adding clear water, lightly stirring, still standing for 3 min, attaching the fertilized ova to clean fernwort, immersing in the aqueous solution of malachite green for 10 min, and hatching in lake water at 13-18.5 deg.C for 140 hr. Its advantages are low cost and high fertilization rate and hatching rate.
Description
Angler
Artificial insemination of whitefish and hatching m
Technical field:
The present invention relates to Yi Zhong Angler
Artificial insemination of whitefish and hatching m belong to fishery-ies product cultural technique field.
Background technology:
Be grown in the peculiar fish — Angler in the Fuxian Lake of Yunnan
Whitefish is one of the four your name fishes in Yunnan, and local people catch it for food over the past thousands of years, have created unique people-fish culture.Angler Qian the eighties
Whitefish is the main product economic fish of Fuxian Lake always, is the people's along the lake pillar industry.After the eighties, since immersion of external fish and excessive fishing for, Dao Zhi Angler
The whitefish population quantity falls sharply, and has now faced the edge of extinction.Hui Fu Angler
The whitefish resource has become the important research contents in Yunnan Province.The Hang Angler of Zhi Zai Jin of the present invention
Artificial insemination of whitefish and hatching research.By literature search, do not see the report identical with the present invention.
Summary of the invention:
The present invention is for recovering the peculiar fish — Angler in the Fuxian Lake of Yunnan
The resource ， Dui Angler of whitefish
Whitefish carries out artificial insemination and hatching.
Angler
Artificial insemination of whitefish and hatching m are made up of wet method fertilization and artificial incubation.Wherein, wet method fertilization is for respectively with sexually matured Ci, Xiong Angler
Ovum of whitefish and sperm squeeze out gently and add a small amount of clear water from its belly, leave standstill 3 minutes after gently stirring with have gentle hands, allow smart ovum fully contact after fertilization, make above the ovum grain evenly sticks to preprepared clean fernwort, begin to carry out artificial incubation; The fish-egg sterilization was carried out in the malachite green aqueous solution immersion that artificial incubation is put into 4ppm concentration for the fernwort that will adhere to fertilized egg earlier in 10 minutes, thoroughly more renew water then, and it is 13-18.5 ℃ in water temperature with lake water, pH value is to carry out the hatching of miniflow water under 8.5 the condition, finishes hatching after 140 hours.
The present invention has that cost is low, easy and simple to handle, fertilization rate and incubation rate advantages of higher.
Embodiment:
In at 6 o'clock in afternoon June 10 calendar year 2001,, catch the Angler of sexual maturity being positioned near Cui Feng township star water stop ground, Jiangchuan County, the Yunnan lower outlet at Fuxian Lake side
Whitefish 10 tails (female 4 tails, male 6 tails).Respectively with sexually matured Ci, Xiong Angler
Ovum of whitefish and sperm squeeze out the back gently from its belly and add a small amount of clear water, leave standstill 3 minutes after gently stirring with have gentle hands, allow smart ovum fully contact after fertilization, make above the ovum grain evenly sticks to preprepared clean fernwort, begin to carry out artificial incubation.Count 9,500 in fertilized egg at 10 o'clock in morning June 11, and fertilization rate is 95%.The fish-egg sterilization was carried out in the malachite green aqueous solution immersion that artificial incubation is put into 4ppm concentration for the fernwort that will adhere to fertilized egg earlier in 10 minutes, thoroughly more renew water then, and be 13-18.5 ℃ with lake water in water temperature, pH value is to carry out the hatching of miniflow water under 8.5 the condition.It is aroused in interest that observe the embryo at 10 o'clock in morning June 13.Observe eyespot at 10 o'clock in morning June 14.Observe major part membrane at 8 o'clock in morning June 15.Add up at 10 o'clock in morning June 16, hatches fry 8835 tails, and incubation rate is 93%.Artificial incubation was finished after 140 hours.
Claims (1)
- Yi Zhong AnglerArtificial insemination of whitefish and hatching m is characterized in that the wet method fertilization is adopted in the artificial insemination in this method, promptly respectively with sexually matured Ci, Xiong Angler
Ovum of whitefish and sperm squeeze out the back gently from its belly and add a small amount of clear water, leave standstill 3 minutes after gently stirring with have gentle hands, allow smart ovum fully contact after fertilization, make above the ovum grain evenly sticks to preprepared clean fernwort, begin to carry out artificial incubation; The fish-egg sterilization was carried out in the malachite green aqueous solution immersion that artificial incubation is put into 4ppm concentration for the fernwort that will adhere to fertilized egg earlier in 10 minutes, thoroughly more renew water then, and it is 13-18.5 ℃ in water temperature with lake water, pH value is to carry out the hatching of miniflow water under 8.5 the condition, finishes hatching after 140 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021132887A CN1181731C (en) | 2002-01-26 | 2002-01-26 | Method for artificial fertilization and hatching of kanglang whitefish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021132887A CN1181731C (en) | 2002-01-26 | 2002-01-26 | Method for artificial fertilization and hatching of kanglang whitefish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1435087A true CN1435087A (en) | 2003-08-13 |
| CN1181731C CN1181731C (en) | 2004-12-29 |
Family
ID=27628390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021132887A Expired - Fee Related CN1181731C (en) | 2002-01-26 | 2002-01-26 | Method for artificial fertilization and hatching of kanglang whitefish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1181731C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613121A (en) * | 2012-04-17 | 2012-08-01 | 中国科学院昆明动物研究所 | Artificial propagating method of semilabeo obscurus |
| CN102757933A (en) * | 2012-08-07 | 2012-10-31 | 中国科学院昆明动物研究所 | Construction method of muscle cell line of anabarilius grahami |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103931538B (en) * | 2014-05-15 | 2015-09-30 | 云南省渔业科学研究院 | The artificial fecundation method of a kind of wild Xichang whitefish |
-
2002
- 2002-01-26 CN CNB021132887A patent/CN1181731C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613121A (en) * | 2012-04-17 | 2012-08-01 | 中国科学院昆明动物研究所 | Artificial propagating method of semilabeo obscurus |
| CN102757933A (en) * | 2012-08-07 | 2012-10-31 | 中国科学院昆明动物研究所 | Construction method of muscle cell line of anabarilius grahami |
| CN102757933B (en) * | 2012-08-07 | 2013-10-23 | 中国科学院昆明动物研究所 | Construction method of muscle cell line of anabarilius grahami |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1181731C (en) | 2004-12-29 |
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