CN1434124A - Method for producing collagen oligopeptide - Google Patents
Method for producing collagen oligopeptide Download PDFInfo
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- CN1434124A CN1434124A CN 03110986 CN03110986A CN1434124A CN 1434124 A CN1434124 A CN 1434124A CN 03110986 CN03110986 CN 03110986 CN 03110986 A CN03110986 A CN 03110986A CN 1434124 A CN1434124 A CN 1434124A
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 38
- 108010035532 Collagen Proteins 0.000 title claims abstract description 38
- 229920001436 collagen Polymers 0.000 title claims abstract description 32
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 21
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 title abstract description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 50
- 230000007062 hydrolysis Effects 0.000 claims abstract description 47
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- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 24
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- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 24
- 108091005804 Peptidases Proteins 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 14
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- 150000001413 amino acids Chemical class 0.000 claims abstract description 7
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- 102000035101 Aspartic proteases Human genes 0.000 claims description 26
- 102000004190 Enzymes Human genes 0.000 claims description 19
- 108090000790 Enzymes Proteins 0.000 claims description 19
- 102000035195 Peptidases Human genes 0.000 claims description 18
- 230000000694 effects Effects 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 230000007935 neutral effect Effects 0.000 claims description 4
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- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 3
- 235000019833 protease Nutrition 0.000 claims 1
- 239000004365 Protease Substances 0.000 abstract description 9
- 239000012670 alkaline solution Substances 0.000 abstract description 4
- 238000011143 downstream manufacturing Methods 0.000 abstract description 4
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- 235000015277 pork Nutrition 0.000 abstract 4
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- 102000004169 proteins and genes Human genes 0.000 description 16
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- 238000006731 degradation reaction Methods 0.000 description 14
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- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
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- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- VHCQVGQULWFQTM-VOTSOKGWSA-N Rubone Chemical compound COC1=CC(OC)=CC(O)=C1C(=O)\C=C\C1=CC(OC)=C(OC)C=C1OC VHCQVGQULWFQTM-VOTSOKGWSA-N 0.000 description 1
- VHCQVGQULWFQTM-UHFFFAOYSA-N Rubone Natural products COC1=CC(OC)=CC(O)=C1C(=O)C=CC1=CC(OC)=C(OC)C=C1OC VHCQVGQULWFQTM-UHFFFAOYSA-N 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- -1 Sumizyme MP Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The method for preparing oligopeptide (number of amino acids contains by peptide is less than 10) by enzymolysis of pork skin collagen is mainly technically characterized by that it uses pork skin collagen as raw material, under the condition of gradually changed pH value without addition of alkali it utilizes the synergistic hydrolysis action of acid protease, alkaline protease or/and neutral protease to obtain oligopeptide. The pork skin raw material for enzymolysis is fresh pork skin, the protease synergistic action hydrolysis condition is: temp. is 30-70 deg.C, time is 2-12 hr, and the substrate concentration is 20-100 g/l. Said invention has no need of adding alkaline solution in hydrolysate in the course of whole hydrolysis, can simplify downstream process and is favorable for reducing production cost of oligopeptide.
Description
Technical field
The invention belongs to the enzyme technology field.Relate to the enzymatic hydrolysis pigskin collagen, provide a kind of under the pH value gradual change condition of exogenously added alkali not, utilize Sumizyme MP and aspartic protease or/and the synergetic hydrolysis effect of neutral protease prepares the method for oligopeptides (less than 10 amino acid whose peptides).
Background technology
Bioactive peptide is the class important function sex factor in the functional health-care food.Bioactive peptide in the market is that the marine organisms albumen valuable protein source such as (as sea cucumbers) with soybean protein isolate, cow's milk, high protein obtains mostly.The product cost height, fetch long price.
China is world-class swine rearing big country and consumption big country, and pigskin accounts for 10% of hog on hook quality, is the maximum byproduct of swine rearing industry.Pigskin is eliminated basically as the raw material of leather industry at present, and it is among the application aspect the food is still being developed.Pigskin collagen is that a kind of waiting of having a large capacity and a wide range exploited natural resources.
Open source literature (meat research; 2000 the 3rd phases; the 41-44 page or leaf) reported; collagen hydrolysate is become the oligopeptides product, can under the situation of not destroying its nutritive value, obtain following functional: protection stomach mucous membrane and antiulcer action, the effect of inhibition increased blood pressure, promotion skin collagen metabolism.In addition, by hydrolysis macromolecular collagen protein is changed into and mainly to contain small-molecular peptides and amino acid whose hydrolyzate, can improve the water-soluble of collagen protein, convenient processing helps widening its food uses.As being that feedstock production goes out similar hydrolyzate with the pigskin collagen, then can open up new way for the reasonable utilization of pigskin.
At present, the method that food protein is hydrolyzed to peptide mainly contains two kinds of acid hydrolyzation and enzymolysis processs.The drawback of acid hydrolyzation is to destroy tryptophane and some hydroxyaminos easily, is difficult to the control hydrolysis degree, and hydrolysate is unfavorable for the oligopeptides preparation based on amino acid.By contrast, the enzymolysis process condition relaxes, and can overcome the shortcoming of acid hydrolyzation.Following Patent publish the concrete grammar of hydrolysis (comprise acid in conjunction with enzymolysis) food protein, as JP8027035A; CN1335405A; The disclosed content of CN1344138A.
The existing disclosed enzyme solution of patent has two characteristics, and the first adopts Sumizyme MP mostly, and it two is all to require in the hydrolytic process constantly to add alkaline solution in hydrolyzed solution, and is constant to keep hydrolyzed solution pH value.
In the alkaline environment of pH6.5-8.5, the hydrolysis efficiency height of Sumizyme MP.But in enzymolysis process, along with constantly opening of protein peptide bond, the carboxyl quantity that of dissociating constantly increases, and the pH value of hydrolyzed solution can constantly descend.Therefore, under the situation of simple use Sumizyme MP, must stablize the pH value by adding alkali lye, thereby keep the hydrolytic activity of Sumizyme MP.Otherwise, be difficult to obtain satisfied raw material (substrate) degradation rate and oligopeptides content.
For the proteic hydrolysis of pigskin collagen, key is to open how as much as possible the peptide bond of collagen protein, improves oligopeptides content.The simple Sumizyme MP of use is stablized under the condition of pH value adding alkali lye, can reach the purpose of raising oligopeptides content.Yet the alkaline solution of keeping the pH value can produce tangible alkaline peculiar smell and introduce salt impurity in hydrolyzate.Therefore, must in downstream processing, adopt complicated desalination to remove miscellaneous operation.This will increase the difficulty of existing method enforcement and the cost of product.
Summary of the invention
The purpose of this invention is to provide a kind ofly under the pH value gradual change condition of exogenously added alkali not, prepare the method for oligopeptides with the enzymic hydrolysis pigskin collagen.Find after deliberation this purpose can be by Sumizyme MP and aspartic protease or/and the synergetic hydrolysis effect of neutral protease realize: at first utilize the hydrolysis by novo pigskin collagen, the pH value of hydrolyzed solution descends gradually along with the quantity of the carrying out of enzyme digestion reaction and free carboxy increases beginning.When the pH of hydrolyzed solution value is lower than the desired lower value of Sumizyme MP, when promptly beginning to present slightly acidic, the hydrolytic activity of Sumizyme MP is suppressed, but aspartic protease or/and neutral protease be activated.So, protein at aspartic protease or/and continue hydrolysis under the effect of neutral protease.Along with open peptide bond quantity continue increase, the pH value of hydrolyzed solution continues to descend, aspartic protease is or/and neutral protease can keep high hydrolytic activity.
Technical scheme of the present invention realizes as described below:
At first, commercially available fresh porcine skin is carried out degrease, rub and give processing, obtain pigskin collagen.Then, in the common fermentation jar that has mechanical stirring device, add an amount of tap water, and require to wherein adding a certain amount of pigskin collagen according to concentration of substrate.Start mechanical stirring arm, make it to stir feed liquid with suitable speed, simultaneously by water jacket or/and water vapor directly contact the reaction material liquid temp mentioned 90 ℃ kept 2-3 minute, make the collagen protein sex change, and then cool to assigned temperature.With pH meter and the pH meter determination of electrode hydrolysis reaction feed liquid potential of hydrogen before that is fixed in the fermentation container.Then, in fermentor tank, add proteolytic enzyme, the beginning hydrolysis reaction.At last, with the variation of pH meter monitoring hydrolyzed solution potential of hydrogen, and analyze peptide molecular weight distribution in different raw material degradation rate, protein degree and the hydrolyzates constantly by sampling (hydrolyzed solution that stirs).After hydrolysis reaction finishes, hydrolyzed solution boiled at 95 ℃ made proteolytic enzyme sex change inactivation, termination reaction in 5-10 minute.
Core of the present invention is the selection and the use of proteolytic enzyme, and the hydrolysising condition that influences the invention process effect comprises concentration of substrate, hydrolysis temperature and time.
Proteolytic enzyme desired pH value condition difference when using can be divided into Sumizyme MP, aspartic protease and neutral protease.
The proteolytic enzyme that the present invention uses comprises Sumizyme MP.Common Sumizyme MP as: alkaline mold protease (derives from aspergillus oryzae, use pH value 6.5-8.5, use temperature 45-60 ℃), alkaline bacterial protease (derives from Bacillus licheniformis, use pH value 6.5-8.5, use temperature 55-70 ℃), the Alcalase alkaline bacterial protease (derives from Bacillus subtilus, use pH value 6.5-8.5, use temperature 55-70 ℃).
The proteolytic enzyme that uses among the present invention also comprises aspartic protease and neutral protease.Common aspartic protease is as: acid mold protease (derive from aspergillus niger, use pH value 2-5.0, use temperature 45-55 ℃), stomach en-(deriving from the animal stomach, use pH value 1.0-3.0, use temperature 30-40 ℃).Common neutral protease as: neutral mold protease (derives from aspergillus oryzae, use pH value 5.0-7.5, use temperature 50-65 ℃), neutral bacteria protease (derives from subtilis, use pH value 4.5-7.0, use temperature 45-60 ℃), the ProtamexTM bacteria protease (belongs to bacillus proteolytic enzyme complex body, use pH value 5.5-7.5, use temperature 55-60 ℃).
In the present invention, Sumizyme MP will be with aspartic protease or/and neutral protease be used.If allow the use temperature scope close, then aspartic protease is or/and neutral protease can add fermentor tank simultaneously with Sumizyme MP.If aspartic protease is or/and the permission use temperature of neutral protease is lower than the use temperature of Sumizyme MP, (the pH value that is hydrolyzed solution stops after the decline) adds aspartic protease again or/and neutral protease after then should disappearing substantially at the hydrolytic activity of Sumizyme MP in fermentor tank.And hydrolysis temperature adjusted to aspartic protease or/and the suitable use temperature of neutral protease.
Or/and neutral protease is used in the scheme, Sumizyme MP can be a kind of enzyme, also can be compound Sumizyme MP at above said Sumizyme MP and aspartic protease.Equally, aspartic protease also can be that combination of acidic proteolytic enzyme is or/and compound neutral protease or/and neutral protease can be a kind of enzyme.
In order to give full play to Sumizyme MP and aspartic protease or/and the concerted catalysis hydrolytic action of neutral protease, Sumizyme MP, aspartic protease are or/and the injected volume of neutral protease (is expressed as enzyme solution and substrate ratio in the present invention, or the enzyme solid accounts for the percentage of substrate weight) can determine by the Utility Engineers's customary way in this field according to its vigor difference.The selection of hydrolysis temperature depends primarily on the use temperature of enzyme.In the mode that Sumizyme MP and aspartic protease or/and neutral protease add simultaneously, it is fixed that hydrolysis temperature should come according to the minimum enzyme of use temperature.In the mode that Sumizyme MP and aspartic protease or/and neutral protease add step by step, hydrolysis temperature can be adjusted according to the permission use temperature of the employed enzyme of different hydrolysis stages.According to the present invention, the range of choice of hydrolysis temperature is 30-70 ℃.
It is hydrolysis material that the present invention adopts fresh porcine skin.Also can adopt and do raw material by the collagen protein that extracts in the pigskin.When the commercially available fresh porcine skin of direct employing is raw material, at first to carry out degreasing and rub handling to it.The present invention adopts the cutter worker to scrape fat.The pigskin of scraping behind the fat shreds with universal cutter, and further rubs one time with civilian mincer.
According to the present invention, the range of choice of concentration of substrate is the 20-100 grams per liter.When concentration of substrate was too high, the hydrolytic activity of enzyme was low, was difficult to obtain satisfied degradation of substrates rate.On the other hand, concentration of substrate is too low, though the hydrolytic activity height of enzyme can increase in the downstream processes difficulty of evaporation, concentrating hydrolysate.When under agitation adding an amount of reaction raw materials in the tap water and being heated to aforesaid temperature range for hydrolysis, the initial pH value of pigskin collagen albumen feed liquid can reach 6.5-7.5, can satisfy the requirement of Sumizyme MP to potential of hydrogen.
According to the present invention, pigskin collagen suitable time scope of hydrolysis under above-described condition is 2-12 hour.Hydrolysis time is unfavorable for obtaining satisfied degradation of substrates rate and protein degree very much in short-term.On the contrary, if hydrolysis time is long, then not only the increase of degradation of substrates rate and protein degree is not obvious, and hydrolyzed solution is rotten under microbial process easily.
The degradation of substrates rate is defined as the per-cent that the pigskin collagen raw material weight that is hydrolyzed accounts for the pigskin collagen raw material weight that feeds intake; Protein degree is defined as the per-cent that the peptide bond quantity of opening accounts for the total peptide bond quantity of protein molecule in hydrolytic process.
The measuring method of degradation rate is as follows: hydrolyzed solution is centrifugal 10min under 4000r/min, supernatant liquid and solids separation, solid insoluble is dried to constant weight under 110 ℃, weighs.Be calculated as follows the degradation of substrates rate then:
Wherein, the pigskin collagen charging capacity is converted to butt.The water content of pigskin collagen is measured after being dried to constant weight under 110 ℃.The degradation of substrates rate is high more, the one way utilization ratio height of expression raw material.
Determination of Hydrolysis Degree of Protein adopts formol titration, concrete measuring method is as follows: with hydrolyzed solution centrifugal 10min under 4000r/min, get the 5ml supernatant liquid, NaOH solution with 0.1M is titrated to pH=8.2, add the formaldehyde solution (used formaldehyde solution is titrated to pH=8.2 with NaOH earlier) of 5ml 37%, the NaOH with 0.1M is titrated to pH=8.2 again.Record is titrated to pH=8.2 institute alkali consumption to solution after adding formaldehyde.The pH value is measured and is used accurate pH meter.Calculate proteinic degree of hydrolysis according to following formula then:
Wherein, at the bottom of the pigskin collagen method of hydrolysis be with pigskin collagen in 6M HCl in 105-110 ℃ of following hydrolysis 24 hours.Proteinic degree of hydrolysis height, the molecular-weight average of expression hydrolyzate is little.
The content of oligopeptides can be measured with product molecular weight distribution in the hydrolyzate.Product molecular weight distribution adopts the sephadex chromatography method to measure: the centrifuged supernatant 1ml that gets the said hydrolyzed thing goes up gel column (SephedaxG-25), elutriant is that the pH value is 8.0 phosphate buffer soln, with peristaltic pump (DESAGA) control flow velocity is 17.3ml/h, effluent liquid is collected with sample run tank (SF-2120), and every pipe collected volume is 1.5ml.Use the absorbancy at ultraviolet spectroscopy 280nm place then, and make the graph of a relation of absorbancy cut.Demarcate the molecular weight distribution of hydrolyzed solution with the molecular weight standard thing.
Effect of the present invention and benefit be, according to the present invention, the degradation rate of pigskin collagen raw material can reach more than 90%, and proteinic degree of hydrolysis can reach about 20%, can reach more than 40% less than 10 amino acid whose oligopeptides in the hydrolyzate.Need not in hydrolyzed solution, add alkaline solution in the whole hydrolytic process of the present invention, therefore simplify the downstream processing technology of product, and helped reducing the production cost of oligopeptides.
Embodiment
Below be described in detail specific embodiments of the invention
Embodiment 1 (comparative example)
Commercially available fresh porcine skin is scraped fat with the cutter worker, be cut into 2 * 2 centimetres of fritters, rub one time with small-sized civilian mincer then.Get 12g and have in the fermentor tank of water jacket, under agitation the temperature in the fermentor tank is brought up to 90 ℃ earlier and kept 5 minutes through pigskin collagen (butt weight) and the adding of 200ml tap water of giving processing.Cool to 60 ℃ of constant temperature then, the pH value that records feed liquid in the fermentor tank by pH meter is about 6.8.Then, in fermentor tank, add 300 μ l Alcalase Sumizyme MPs (2.4L, sign vigor are the 2.4AU/ gram, and Denmark NOVO company produces), under constantly stirring, begin hydrolysis.In this example, concentration of substrate is 60 gram pigskin collagen/premium on currency, and the ratio of enzyme-to-substrate is 20 μ l Alcalase alkalescence enzyme (2.4L)/gram pigskin collagen.Total hydrolysis time is 12 hours.In hydrolytic process, pH value, degradation of substrates rate and protein degree are carried out trace analysis.Analytical results is: hydrolyzed solution pH value increases after hydrolysis begins to reach 6.3-6.4 in 1 hour slowly, and hydrolysis then stopped later on increasing in 4 hours.Degradation of substrates rate and protein degree also increase slowly after hydrolysis begins to reach respectively in 1 hour about 84% and about 8.4%, and hydrolysis stops after 4 hours increasing.
Embodiment 2
Repeat embodiment 1, but when adding 300 μ l Alcalase Sumizyme MPs, add the aspergillus niger aspartic protease (enzyme activity 3000u/g) of 4% (is benchmark with substrate butt weight).Analytical results is: hydrolyzed solution pH value continues to descend in hydrolysis time, and hydrolysis is carried out reaching about 5.3 after 12 hours.The degradation of substrates rate reached maximum after 4 hours about 94.7%, after this changes not quite, and protein degree continued to increase in the investigation time, and when hydrolysis time reached 12 hours, degree of hydrolysis was increased to about 17.7%.Record with the sephadex chromatography method, accounted for 40% of peptide content less than 10 amino acid whose oligopeptides in the resulting hydrolyzate in 12 hours in hydrolysis.
Embodiment 3
Repeat embodiment 2, but the aspergillus niger aspartic protease just adds fermentor tank after the Alcalase Sumizyme MP adds 1 hour, and the temperature of fermentor tank is adjusted into 55 ℃.Then 12 hours result of hydrolysis is: hydrolyzed solution pH value is about 5.1, and the degradation of substrates rate is about 94%, and protein degree is about 16%.
Embodiment 4
Repeat embodiment 2, add 1% Protamex but change
TMNeutral bacteria protease (belong to bacillus proteolytic enzyme complex body, enzyme mark vigor is 1.5 AU/ grams) and 1% aspergillus niger aspartic protease replace added 2% aspergillus niger aspartic protease originally.Hydrolysis 4 hours, 8 hours, 12 hours result such as following table then:
| Hydrolysis time (hour) | ????4 | ????8 | ????12 |
| Hydrolyzed solution pH value | ????5.6 | ????5.2 | ????5.0 |
| Rate (%) falls in substrate | ????92 | ????93 | ????93 |
| Protein degree (%) | ????13 | ????15 | ????17 |
Embodiment 5
Repeat embodiment 2, but the pigskin collagen raw material changes by the collagen protein that extracts in the pigskin into.Then 12 hours result of hydrolysis is: hydrolyzed solution pH value is about 4.5, and the degradation of substrates rate is about 100%, and protein degree is about 21%.
Claims (4)
1. method of producing collagen oligopeptide, it is the method for preparing oligopeptides (less than 10 amino acid whose peptides) from pigskin collagen, under the pH value gradual change condition of exogenously added alkali not, utilize Sumizyme MP and aspartic protease or/and the synergy hydrolysis pigskin collagen of neutral protease, it is characterized in that, Sumizyme MP refers to alkaline fungous enzyme, alkalescence bacterial enzyme and any mixture thereof, aspartic protease refers to acid fungous enzyme, stomach en-and any mixture thereof, neutral protease refers to neutral fungous enzyme, neutral bacterial enzyme and any mixture thereof.
2. a kind of method of producing collagen oligopeptide according to claim 1 is characterized in that, Sumizyme MP and aspartic protease are or/and the adding mode of neutral protease comprises that adding simultaneously and substep add.
3. a kind of method of producing collagen oligopeptide according to claim 1 is characterized in that the pigskin raw material that is used for enzymolysis is a fresh porcine skin.
4. a kind of method of producing collagen oligopeptide according to claim 1 is characterized in that, said proteinase synergy effect hydrolysising condition is temperature 30-70 ℃, time 2-12 hour.Concentration of substrate 20-100 grams per liter.
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| CN 03110986 CN1209465C (en) | 2003-01-30 | 2003-01-30 | Method for producing collagen oligopeptide |
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|---|---|---|---|
| CN 03110986 CN1209465C (en) | 2003-01-30 | 2003-01-30 | Method for producing collagen oligopeptide |
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| CN1209465C CN1209465C (en) | 2005-07-06 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434529C (en) * | 2005-04-22 | 2008-11-19 | 黄磊 | III type proteinic small peptide and preparation technique thereof |
| CN1974783B (en) * | 2006-12-07 | 2010-09-01 | 华南理工大学 | Biocatalytic process for preparing collagen peptide |
| CN102154420A (en) * | 2010-11-11 | 2011-08-17 | 武汉远城科技发展有限公司 | Method for extracting pigskin collagen peptides |
| CN102517367A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Method for preparing collagen anti-oxidation peptide by using pig skin |
| CN102578363A (en) * | 2012-03-29 | 2012-07-18 | 江南大学 | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage |
| CN103060413A (en) * | 2013-01-15 | 2013-04-24 | 青岛贝尔特生物科技有限公司 | Method for preparing collagen oligopeptide through mixed enzyme |
| CN108949885A (en) * | 2018-08-16 | 2018-12-07 | 东北农业大学 | A kind of pair of osteoblastic proliferation has the preparation method and application of the pigskin gelatin peptide of facilitation |
-
2003
- 2003-01-30 CN CN 03110986 patent/CN1209465C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100434529C (en) * | 2005-04-22 | 2008-11-19 | 黄磊 | III type proteinic small peptide and preparation technique thereof |
| CN1974783B (en) * | 2006-12-07 | 2010-09-01 | 华南理工大学 | Biocatalytic process for preparing collagen peptide |
| CN102154420A (en) * | 2010-11-11 | 2011-08-17 | 武汉远城科技发展有限公司 | Method for extracting pigskin collagen peptides |
| CN102154420B (en) * | 2010-11-11 | 2013-05-22 | 湖北远成药业有限公司 | Method for extracting pigskin collagen peptides |
| CN102517367A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Method for preparing collagen anti-oxidation peptide by using pig skin |
| CN102517367B (en) * | 2011-12-15 | 2014-04-02 | 河南科技大学 | Method for preparing collagen anti-oxidation peptide by using pig skin |
| CN102578363A (en) * | 2012-03-29 | 2012-07-18 | 江南大学 | Method for preparing small-molecule collagen polypeptide powder by using poultry skin and application of small-molecule collagen polypeptide powder in fruit juice beverage |
| CN103060413A (en) * | 2013-01-15 | 2013-04-24 | 青岛贝尔特生物科技有限公司 | Method for preparing collagen oligopeptide through mixed enzyme |
| CN108949885A (en) * | 2018-08-16 | 2018-12-07 | 东北农业大学 | A kind of pair of osteoblastic proliferation has the preparation method and application of the pigskin gelatin peptide of facilitation |
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