CN100434529C - III type proteinic small peptide and preparation technique thereof - Google Patents
III type proteinic small peptide and preparation technique thereof Download PDFInfo
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- CN100434529C CN100434529C CNB2005100658638A CN200510065863A CN100434529C CN 100434529 C CN100434529 C CN 100434529C CN B2005100658638 A CNB2005100658638 A CN B2005100658638A CN 200510065863 A CN200510065863 A CN 200510065863A CN 100434529 C CN100434529 C CN 100434529C
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Abstract
The present invention relates to an III type protein small peptide. The main components of the present invention are proteins; the protein content of the present invention is 90% or more than 90%; the molecular weight of the proteins is smaller than 2500D. The III type protein small peptide has small protein molecular weight, the proteins are easily absorbed by human bodies, and ox embryos and pigtails are adopted in preparation by adopting an advanced biologic enzymolysis method; extracted collagen is rich in nutrients, has small molecular weight and is especially suitable for skin maintenance; simultaneously, the preparation technique is scientific and reasonable, has the advantages of simple and easy operation, high efficiency and low cost and is suitable for industrial production.
Description
Technical field
The present invention relates to field of biological product, especially a kind of protein small peptide and preparation technology thereof.
Background technology
Collagen protein also meets the theory of " dirty with dirty benefit " of the traditional Chinese medical science always as the good merchantable brand of skin care.Collagen protein in the present commercially available makeup all is that general animal skin is made raw material and made after enzymic hydrolysis, and molecular weight is greater than 5000D.This molecular weight has certain anaphylactogen characteristic greater than the collagen protein of 5000D, after allergic constitution uses, redness can occur, play rash, even anaphylaxis such as ulcer, and is serious even disfeature.Therefore, there is potential harm in the used for cosmetic of main component that traditional with the collagen protein is in skin care.
Summary of the invention
The purpose of this invention is to provide a kind of protein small peptide, protein molecular weight is little, easily by skin absorption, does not have hazardness, and raw material is good, and preparation technology's science, reasonable is fit to suitability for industrialized production.
Technical solution of the present invention is:
A kind of protein small peptide, main component are protein, and protein content is that protein molecular weight is less than 2500D more than 90% or 90%.
As a kind of preferred version, the protein molecular weight of protein small peptide of the present invention is less than 1000D.
The preparation technology of protein small peptide, raw material is oxtail and/or pigtail, processing step is:
A, oxtail, pigtail are cut into chunks, and separate glue wherein after the boiling;
B, with above-mentioned glue, adjust temperature to 40 ℃-60 ℃, transfer pH value 7.5-9, add compound protease and carry out enzymolysis; The conjugated protein enzyme dosage is the 0.2-1% of the contained total protein of glue, enzymolysis time: 3-5h; The enzymolysis solution enzyme that goes out, enzyme-removal temperature is 85-100 ℃, goes out 10-20 minute enzyme time; Go out enzyme rear decoloring, filtration are filtered rear decoloring liquid and are used molecular sieve filtration again, concentrate then, be drying to obtain.
As a kind of preferred version, when raw material is oxtail and pigtail, oxtail consumption 30%-70%, pigtail consumption 30%-70%.
As a kind of preferred version, the segment length 3-8 that ox, pigtail are cut into chunks in the steps A centimetre.
As a kind of preferred version, boiling autoclave after ox, pigtail are cut into chunks in the steps A, amount of water 1-2 times, vapor pressure 1.5-4kg, cooking time 2-5h.
As a kind of preferred version, enzymolysis solution goes out and is cooled to 20 ℃-80 ℃ behind the enzyme among the step B, adds gac 1.5-3% decolouring, time 30-60 minute.
As a kind of preferred version, the hydrolyzed solution among the step B after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 4 * 48 * 8 pure cotton canvas for use, and pure cotton canvas uses after 0.2%-1% dilute alkaline soln degreasing post rinsing is clean; Filter with microporous membrane is smart behind the Plate Filtration.
As a kind of preferred version, the drying among the step B is vacuum-drying or spraying drying; During spraying drying, the equipment importation hot blast temperature is 90 ℃-140 ℃, and the outlet hot blast temperature is 30 ℃-70 ℃.
Protein small peptide provided by the invention, protein content are more than 90% or 90%, and protein molecular weight is less than 2500D, below the preferred 1000D.Produce with ox, pigtail, ox, pig health are unique to be tail not stopping transport moving, and the collagen protein in the tail is fit to the maintenance of skin very much.Pass through advanced compound protease enzyme solution in the preparation, through molecular sieve filtration, the gained protein small peptide, molecular weight is little, and is nutritious, and no anaphylaxis is the good merchantable brand of skin care.Simultaneously, preparation technology is scientific and reasonable, and is easy to operation, the efficient height, and cost is low, is suitable for suitability for industrialized production.
Embodiment
Embodiment one:
Be equipped with 30 kilograms of cow's tails, 70 kilograms of pigtails.
Ox, pigtail are cut into 3 centimetres section, use the autoclave boiling, add 100 kilograms in water, vapor pressure 1.5kg, cooking time 2h, the glue that separates wherein after finishing is standby.Contain 9 kilograms in albumen (containing 9 kilograms of calculating of albumen) in the glue by per hundred kilograms of tails
With above-mentioned glue, adjust temperature to 40 ℃, transfer pH value 7, add compound protease (directly the buying commodity) enzymolysis that Lingwu, Ningxia safe fortune biochemical product company limited produces.Containing total protein in the said mixture matter is 9 kilograms, and the conjugated protein enzyme dosage is 18 grams.Concentration of substrate is 10% during enzymolysis, the system pH value: initial 8.0, terminal point 6.5, and temperature: 40 ℃, enzymolysis time 3h; The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 85 ℃, goes out 10 minutes enzyme time; Going out is cooled to 20 ℃ after enzyme is finished, and adds gac 135 gram decolourings, 30 minutes time.Hydrolyzed solution after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 4 * 4 pure cotton canvas for use, and pure cotton canvas uses after 0.2% dilute alkaline soln degreasing post rinsing is clean.Filter with microporous membrane is smart again behind the Plate Filtration.Smart filter rear decoloring liquid molecular sieve filtration concentrates with economic benefits and social benefits falling film type thickner then.Concentrate the back and carry out spraying drying in the pressure spray dryer tower, the import hot blast temperature is 90 ℃ during spraying, and the outlet hot blast temperature is 30 ℃.
Make 8.6 kilograms of protein small peptide finished products (yield 95%) after above-mentioned drying step is finished, protein content is 90%, and protein molecular weight is less than 2500D.
Embodiment two:
Be equipped with 70 kilograms of cow's tails, 30 kilograms of pigtails.
Ox, pigtail are cut into 6 centimetres section, use the autoclave boiling, add 200 kilograms in water, vapor pressure 3kg, cooking time 3.5h, the glue that separates wherein after finishing is standby.Contain 9 kilograms in albumen (containing 9 kilograms of calculating of albumen) in the glue by per hundred kilograms of tails
With above-mentioned glue, adjust temperature to 50 ℃, transfer pH value 8, add compound protease (directly the buying commodity) enzymolysis that Novi's letter is produced.Containing total protein in the said mixture matter is 9 kilograms, and the conjugated protein enzyme dosage is 72 grams.Concentration of substrate is 12% during enzymolysis, the system pH value: initial 8.2, terminal point 6.8, and temperature: 50 ℃, enzymolysis time 4h; The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 90 ℃, goes out 15 minutes enzyme time; Going out is cooled to 50 ℃ after enzyme is finished, and adds gac 180 gram decolourings, 45 minutes time.Hydrolyzed solution after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 6 * 6 pure cotton canvas for use, and pure cotton canvas uses after 0.8% dilute alkaline soln degreasing post rinsing is clean.Filter with microporous membrane is smart again behind the Plate Filtration.Smart filter rear decoloring liquid molecular sieve filtration concentrates with economic benefits and social benefits falling film type thickner then.Concentrate the back and carry out spraying drying in the pressure spray dryer tower, the import hot blast temperature is 90 ℃ during spraying, and the outlet hot blast temperature is 30 ℃.
Make 8.8 kilograms of protein small peptide finished products (yield 98%) after above-mentioned drying step is finished, protein content is 95%, and protein molecular weight is less than 1800D.
Embodiment three:
Be equipped with 100 kilograms of cow's tails.
Cow's tail is cut into 8 centimetres section, uses the autoclave boiling, add 300 kilograms in water, vapor pressure 4kg, cooking time 5h, the glue that separates wherein after finishing is standby.Contain 9 kilograms in albumen (containing 9 kilograms of calculating of albumen) in the glue by per hundred kilograms of tails
With above-mentioned glue, adjust temperature to 60 ℃, transfer pH value 9, add compound protease (directly the buying commodity) enzymolysis that Guangzhou distant sky zymin factory produces.Containing total protein in the said mixture matter is 9 kilograms, and the conjugated protein enzyme dosage is 90 grams.Concentration of substrate is 15% during enzymolysis, the system pH value: initial 8.5, terminal point 6.9, and temperature: 60 ℃, enzymolysis time 5h; The enzyme that goes out behind the enzymolysis, enzyme-removal temperature is 100 ℃, goes out 20 minutes enzyme time; Going out is cooled to 80 ℃ after enzyme is finished, and adds gac 270 gram decolourings, 60 minutes time.Hydrolyzed solution after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected 8 * 8 pure cotton canvas for use, and pure cotton canvas uses after 1% dilute alkaline soln degreasing post rinsing is clean.Filter with microporous membrane is smart again behind the Plate Filtration.Smart filter rear decoloring liquid molecular sieve filtration concentrates with economic benefits and social benefits falling film type thickner then.Concentrate the back and carry out spraying drying in the pressure spray dryer tower, the import hot blast temperature is 140 ℃ during spraying, and the outlet hot blast temperature is 70 ℃.
Make 8.3 jin of protein small peptide finished products (yield 92%) after above-mentioned drying step is finished, protein content is 99%, and protein molecular weight is less than 1000D.
Embodiment four:
With 100 kilograms of pigtails, produce with reference to embodiment three, make 8.1 jin of protein small peptide finished products (yield 90%), protein content is 97%, protein molecular weight is less than 500D.
Claims (7)
1, a kind of preparation technology of protein small peptide, raw material is oxtail and/or pigtail, processing step is:
A, oxtail, pigtail are cut into chunks, and separate glue wherein after the boiling;
B, with above-mentioned glue, adjust temperature to 40 ℃-60 ℃, transfer pH value 7.5-9, add compound protease and carry out enzymolysis; The conjugated protein enzyme dosage is the 0.2-1% of the contained total protein of glue, enzymolysis time: 3-5h; The enzymolysis solution enzyme that goes out, enzyme-removal temperature is 85-100 ℃, goes out 10-20 minute enzyme time; Go out enzyme rear decoloring, filtration are filtered rear decoloring liquid and are used molecular sieve filtration again, concentrate then, be drying to obtain;
The protein small peptide main component of producing is a protein, and protein content is that protein molecular weight is less than 2500D more than 90% or 90%.
2, technology as claimed in claim 1, when raw material is oxtail and pigtail, oxtail consumption 30%-70%, pigtail consumption 30%-70%.
3, technology as claimed in claim 1, the segment length 3-8 that ox, pigtail are cut into chunks in the steps A centimetre.
4, technology as claimed in claim 1, boiling autoclave after ox, pigtail are cut into chunks in the steps A, amount of water 1-2 times, vapor pressure 1.5-4kg, cooking time 2-5h.
5, technology as claimed in claim 1, enzymolysis solution goes out and is cooled to 20 ℃-80 ℃ behind the enzyme among the step B, adds gac 1.5-3% decolouring, time 30-60 minute.
6, technology as claimed in claim 1, the hydrolyzed solution among the step B after the decolouring filters with plate-and-frame filter press, and filter material cloth is selected the pure cotton canvas of 4 * 4-8 * 8 for use, and pure cotton canvas uses after 0.2%-1% dilute alkaline soln degreasing post rinsing is clean; Filter with microporous membrane is smart behind the Plate Filtration.
7, technology as claimed in claim 1, the drying among the step B are vacuum-drying or spraying drying; During spraying drying, the equipment importation hot blast temperature is 90 ℃-140 ℃, and the outlet hot blast temperature is 30 ℃-70 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100658638A CN100434529C (en) | 2005-04-22 | 2005-04-22 | III type proteinic small peptide and preparation technique thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100658638A CN100434529C (en) | 2005-04-22 | 2005-04-22 | III type proteinic small peptide and preparation technique thereof |
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| CN1687441A CN1687441A (en) | 2005-10-26 |
| CN100434529C true CN100434529C (en) | 2008-11-19 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101717804B (en) * | 2009-12-11 | 2012-07-04 | 四川大学 | Method for preparing medical III type collagen |
| CN101781672A (en) * | 2010-04-13 | 2010-07-21 | 北京华达杰瑞生物技术有限公司 | Process for preparing hydrolyzed II-type collagen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1351083A (en) * | 2000-11-01 | 2002-05-29 | 四川大学 | Process for preparing collagen |
| CN1434124A (en) * | 2003-01-30 | 2003-08-06 | 大连理工大学 | Method for producing collagen oligopeptide |
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2005
- 2005-04-22 CN CNB2005100658638A patent/CN100434529C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1351083A (en) * | 2000-11-01 | 2002-05-29 | 四川大学 | Process for preparing collagen |
| CN1434124A (en) * | 2003-01-30 | 2003-08-06 | 大连理工大学 | Method for producing collagen oligopeptide |
Non-Patent Citations (4)
| Title |
|---|
| PH值渐变条件下双酶协同水解猪皮制备胶原蛋白寡肽的研究. 李岩等.食品科学，第24卷第7期. 2003 |
| PH值渐变条件下双酶协同水解猪皮制备胶原蛋白寡肽的研究. 李岩等.食品科学，第24卷第7期. 2003 * |
| 酶解胶原蛋白多肽的制备与分析. 李彦春等.中国皮革，第33卷第5期. 2004 |
| 酶解胶原蛋白多肽的制备与分析. 李彦春等.中国皮革，第33卷第5期. 2004 * |
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