CN1433788A - Method for extracting effective active component of rodgersia aesculifolia and use thereof - Google Patents
Method for extracting effective active component of rodgersia aesculifolia and use thereof Download PDFInfo
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- CN1433788A CN1433788A CN02101992A CN02101992A CN1433788A CN 1433788 A CN1433788 A CN 1433788A CN 02101992 A CN02101992 A CN 02101992A CN 02101992 A CN02101992 A CN 02101992A CN 1433788 A CN1433788 A CN 1433788A
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Abstract
The present invention relates to a method for extracting effective active component of Chinese medicinal material rodgersia aesculifolia and its application in preparation of medicine for resisting virus and inhibiting and killing bacteria. Its preparation method includes the following steps: a. using root tuber of the rodgersia aesculifolia as raw material, slicing, cleaning, drying and pulverizing; b. using organic solution whose concentration is 45-95% to make extraction, separating polar region, using organic solution to make extraction, making activity test, screening and removing residue, its extraction mode can be cold-impregnation or thermal reverse-flow; c. reducing pressure and recovering solvent, extracting, concentrating to obtain the extractum.
Description
(1) technical field:
The present invention relates to a kind of extracting method and the application of effective active component of rodgersia aesculifolia in preparation antiviral and antibacterial Bactericidal medicine of effective active component of rodgersia aesculifolia.
(2) background technology:
At present, it is antiviral agents that some Western medicine and Chinese patent medicine were once arranged both at home and abroad, and the former has certain side effect after the patient is taken, and the latter is not obvious to patient's curative effect.
Show that according to the World Health Organization's whole world virus monitor research report Coxsackie virus infects the most common in the crowd.In neonate, child's sudden death case, 20% is due to the viral myocarditis.Owing to can in cardiac muscle, continue behind the viral infection to exist, still lack effective antiviral means [1] at present.
[1] " healthy newspaper " 2001,03,20, Jiang Ming.
Now studied and found out, herpangina (also claiming herpangina) mostly is and infects due to the Coxsackie virus.Onset just has headache, child cry, general pain, similar cold symptoms such as fever, then find stomachache, refusing to eat, gastrointestinal symptoms such as vomiting, the child is sick high fever therefore, body constitution descends, even occurs fainting from fear, and concurrent parotitis and mumps meningitis are to life constitute a threat to [2].
[2] " Fujian health newspaper " 2000,09,24 Jin Ruomi.
(3) summary of the invention:
The object of the present invention is to provide a kind of extracting method of effective active component of rodgersia aesculifolia.
Another object of the present invention is to provide the purposes of effective active component of rodgersia aesculifolia in preparation antiviral and antibacterial Bactericidal medicine.
The extracting method of effective active component of rodgersia aesculifolia of the present invention, this method may further comprise the steps:
A. the tuber with rodgersia aesculifolia is a raw material, it is selected, section, clean, dry, the granularity that is crushed to 20 orders, 10 orders or 3-5mm;
B. be that 45-95% organic solution always extracts with concentration, wherein organic solution can be that concentration is any of 45～60%, 70～75%, 90～95% medical ethanol; Polarity position organic extractant solution then carries out activity test then, and organic solution can be chloroform (acetone), ethyl acetate, n-butyl alcohol; Cooperate pharmacological screening, discard residue; Wherein the organic solution consumption be material quantity 4-8 doubly, the extraction mode can be merceration or hot widely different stream.The merceration time of merceration mode is each 5-9 days, and extraction times is 3-5 time, and extraction temperature is 12-30 ℃.The widely different stream time of the widely different stream mode of heat is each 1-4 hour, preferred 2-4 hour; Widely different stream number of times is 2-5 time, preferred 2-3 time;
C. behind the decompression and solvent recovery, extracting solution is condensed into extractum;
D. chromatographic isolation is carried out with chromatographic column in the branch position, column chromatography is made filler with polyamide or with silica gel, with chloroform: ethyl acetate: formic acid=5: 4: 0.1 or n-butyl alcohol: chloroform: formic acid=8: 3: 1 is eluant, the eluent of active site, promptly obtain the effective active composition.
Effective active component of rodgersia aesculifolia comprises 1) Rodgersia podophylla A. Gray first element; 2) Rodgersia podophylla A. Gray second element; 3) Quercetin; 4) Arbutin; 5) syringic acid; 6) gallic acid; 7) 3-O-galloyl-(-)-epicatechin; 8) (3-O-galloyl)-epicatechin-(4 β-8)-(3-O-galloyl)-epicatechin; 9) 1,2,4,6-four-O-3-O-galloyl-beta-D-glucose; 10) gallicin; 11) linalool; 12) 2,6-dihydroxyphenyl acetic acid methyl ester etc.
The difficult and complicated illness that the effective active composition of pure Chinese medicine rodgersia aesculifolia causes Coxsackie virus, herpesvirus etc. has obvious curative effects, suitable treatment viral myocarditis, angina, parotitis, herpetic stomatitis and malignant dysentery, hepatitis B, influenza etc., this medication is imitated significantly, take safety, have no side effect, enhance immunity has overcome the shortcoming of existing Western medicine, Chinese patent medicine.The effective active composition of rodgersia aesculifolia of the present invention can use separately, also can with the fluid extract of the active component that extracts in the rodgersia aesculifolia separately and adjuvant (as, medical starch, ethyl cellulose, gelatin etc.) mix homogeneously, drying, pulverize, sieve, make required dosage form after ripening and the sterilization, can treat by the caused difficult and complicated illness of Coxsackie virus, herpes simplex virus and encephalitis b virus, as viral myocarditis, parotitis, angina, herpetic stomatitis etc.Also can be with behind itself and auxiliary agent, the adjuvant science compatibility, be dried and crushed into 100-120 purpose fine powder, carry out ripening, sterilization treatment 10-30 minute under 100-110 ℃ of temperature, meaning promptly can be made into capsule, buccal tablet and also can be made into unguentum, both, made corresponding medicament according to different auxiliary agents.For example, add a kind of or several auxiliary agents are made antiviral, antibacterial sterilization preparation, or control medicaments such as hepatitis B, asthma, influenza.Made medicine has the safety of taking, and curative effect is obvious, has no side effect, and antiviral, antibacterial sterilization scope are wide, and preparation method is simple and easy to do, advantages such as non-environmental-pollution.
Contain rich nutrient contents in the effective active composition medicament of rodgersia aesculifolia.In rodgersia aesculifolia, the content of its total amino acids is 2.01%.Identify the aminoacid of 18 kinds of needed by human body, wherein had 8 kinds to be needed by human body and can not self synthetic aminoacid.
Effective active component of rodgersia aesculifolia also has strong killing action to kind of pathogenic bacteria surplus staphylococcus aureus, Li Side bacillus, yersinia, Bacillus proteus, pseudomonas aeruginosa, anthrax bacillus, Bacillus typhi, the bacterium flexneri etc. 10.DNA (HGV herpes simplex) virus can not only be killed and suppress to this medicine, but and RNA (Ke Saji) virus of can also going out, illustrate that this medicine is the broad spectrum activity antiviral drugs.This medicine safe dose is 192-96mg/Kg, but and the virus of when containing 17-34mg/Kg, can going out, simultaneously the cell of the cell pipe after the kill virus still can continue division and goes down to posterity, and illustrates that this medicine is safe and effective, toxicity is little.
Plant white mice with Switzerland and carry out per os filling stomach viral infection test, with the distilled water contrast, experimental result shows that the effective active composition per os equivalent of rodgersia aesculifolia is irritated stomach (0.01ml/g), and the half of its acute oral causes death greater than 12500mg/Kg.By oral toxicity split pole standard, belong to little poison (actual nontoxic).In addition, test shows that also hepatitis B virus and influenza virus are had 100% inhibitory action.The effective active composition of rodgersia aesculifolia is made medicament, to treating by the caused difficult and complicated illness of Coxsackie virus, herpes simplex virus and encephalitis b virus, as viral myocarditis, parotitis, angina, herpetic stomatitis etc., effective percentage 93.7%, acceptance rate 86%.
(5) specific embodiment:
The following examples can make those skilled in the art more fully understand the present invention, but do not limit the present invention in any way.
Embodiment 1:
Get the tuber 200g of rodgersia aesculifolia, it is selected, section, clean, dry, be crushed to 10 purpose granularities; With the concentration of 1000g is that 45～60% medical ethanol always extracts, and the extraction mode is the merceration mode, and the merceration time is each 7 days, and extraction times is 4 times, and extraction temperature is 25 ℃.Polarity position organic extractant solution then, organic solution can be chloroform, ethyl acetate, n-butyl alcohol; Then carry out activity test, cooperate pharmacological screening, discard residue; Behind the decompression and solvent recovery, extracting solution is condensed into extractum; Chromatographic isolation is carried out with chromatographic column in the branch position, and column chromatography is made filler with silica gel, and with chloroform: ethyl acetate: formic acid=12: 5: 0.1 is eluant, the eluent of active site, promptly obtain the effective active composition.
Embodiment 2:
Get the tuber 150g of rodgersia aesculifolia, it is selected, section, clean, dry, the granularity that is crushed to 3-5mm; With the concentration of 750g amount is that 70～75% medical ethanol always extracts, and the extraction mode is hot widely different stream mode, and the widely different stream time is each 3 hours; Widely different stream number of times is 3 times.Polarity position organic extractant solution then, organic solution can be chloroform, ethyl acetate, n-butyl alcohol; Then carry out activity test, cooperate pharmacological screening, discard residue; Behind the decompression and solvent recovery, extracting solution is condensed into extractum; Chromatographic isolation is carried out with chromatographic column in the branch position, and column chromatography is made filler with polyamide, and with n-butyl alcohol: chloroform: formic acid=8: 3: 1 is eluant, the eluent of active site, promptly obtain the effective active composition.
Claims (6)
1. the extracting method of an effective active component of rodgersia aesculifolia, this method may further comprise the steps:
A. the tuber with rodgersia aesculifolia is a raw material, it is selected, section, clean, dry, the granularity that is crushed to 20 orders, 10 orders or 3-5mm;
B. be that 45-95% organic solution always extracts with concentration, polarity position organic extractant solution then carries out activity test then, cooperates pharmacological screening, discards residue; Wherein the organic solution consumption be material quantity 4-8 doubly, the extraction mode can be merceration or hot widely different stream;
C. behind the decompression and solvent recovery, extracting solution is condensed into extractum;
D. chromatographic isolation is carried out with chromatographic column in the branch position, column chromatography is made filler with polyamide or with silica gel, with chloroform: ethyl acetate: formic acid=5: 4: 0.1 or n-butyl alcohol: chloroform: formic acid=8: 3: 1 is eluant, the eluent of active site, promptly obtain the effective active composition.
2. the extracting method of effective active component of rodgersia aesculifolia according to claim 1, wherein the organic solution of total extraction usefulness can be that concentration is any of 45～60%, 70～75%, 90～95% medical ethanol in the b step; The organic solution of polarity position extraction usefulness can be chloroform (acetone), ethyl acetate, n-butyl alcohol.
3. the extracting method of effective active component of rodgersia aesculifolia according to claim 1, wherein the extraction mode in the b step is a merceration, and the merceration time is each 5-9 days, and extraction times is 3-5 time, and extraction temperature is 12-30 ℃.
4. the extracting method of effective active component of rodgersia aesculifolia according to claim 1, wherein the extraction mode in the b step is hot widely different stream, and the widely different stream time is each 1-4 hour, and widely different stream number of times is 2-5 time.
5. the extracting method of effective active component of rodgersia aesculifolia according to claim 1, wherein the extraction mode in the b step is hot widely different stream, and the widely different stream time is each 2-4 hour, and widely different stream number of times is 2-3 time.
6. the application of effective active component of rodgersia aesculifolia in preparation antiviral and antibacterial Bactericidal medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021019924A CN1256116C (en) | 2002-01-24 | 2002-01-24 | Method for extracting effective active component of rodgersia aesculifolia and use thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021019924A CN1256116C (en) | 2002-01-24 | 2002-01-24 | Method for extracting effective active component of rodgersia aesculifolia and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1433788A true CN1433788A (en) | 2003-08-06 |
| CN1256116C CN1256116C (en) | 2006-05-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB021019924A Expired - Fee Related CN1256116C (en) | 2002-01-24 | 2002-01-24 | Method for extracting effective active component of rodgersia aesculifolia and use thereof |
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- 2002-01-24 CN CNB021019924A patent/CN1256116C/en not_active Expired - Fee Related
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