CN1431314A - Lipopeptide biosurfactant and its application in making domestic garbage turn to compost - Google Patents
Lipopeptide biosurfactant and its application in making domestic garbage turn to compost Download PDFInfo
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- CN1431314A CN1431314A CN03118039A CN03118039A CN1431314A CN 1431314 A CN1431314 A CN 1431314A CN 03118039 A CN03118039 A CN 03118039A CN 03118039 A CN03118039 A CN 03118039A CN 1431314 A CN1431314 A CN 1431314A
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- lipopeptid
- compost
- active agent
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- subtilis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
A biological surfactant lipopetide is prepared from hay bacillus through fermenting culture by use of plant oil as carbon source to obtain fermented lipopeptide solution, centrifugal removing thallus, and proportionally diluting. It can be directly used for compost to improve the efficiency and quality of compost.
Description
Technical field: the present invention relates to a kind of tensio-active agent, be specifically related to the bio-surfactant and the application thereof of microorganisms.
Background technology: the improvement of composting process medium physicochemical condition can be by the C/N value, humidity, oxygen, temperature, porosity etc. of control compositing system.The application of these means in composting process is necessary, but the purport of these methods all is to improve mass transfer or favourable microorganism growth, and in the interaction of improvement microorganism and medium and directly promote microorganism aspect the decomposition course of hardly degraded organic substance, to have little scope for one's talents.At present domestic have certain research to biological overall situation improvement aspect in the composting process, and technical patent relates to aspects such as novel appts design and improvement.The research aspect the microenvironment improving technology then instrument be the physics method improvement (as using flyash) of medium, the research and development aspect that is used for the bio-surfactant of compost does not see as yet that then patent report is arranged.
There is very big shortcoming in traditional chemical surfactant: because it cannot be degraded by microorganisms, therefore, may bring new pollution to environment, might suppress microbic activity in the compost simultaneously.And bio-surfactant to be vital movement by microorganism form, it does not have toxic action to microorganism, not have the danger of being degraded too early by microorganism as preferential degraded object simultaneously yet, therefore, can be used for the improvement of composting process microenvironment.
Because bio-surfactant has many good character, therefore Mi Yue is subjected to people's attention more.According to constitutional features, bio-surfactant can fall into 5 types: glycolipid, lipopeptid, polyose-protein complex compound, phosphatide and lipid acid or neutral fat.Wherein, lipopeptid is the very strong bio-surfactant of a kind of surfactivity, be by some genus bacillus under aerobic condition, be the secondary metabolite that carbon source through fermentation produces with hydrophobic nature matrix.At present, Buddhist Sha graceful (sufactin) research that produces with the subtilis (Bacillus subtilis ATCC 21332) of American type culture collection (ATCC) at most.
Yet, because lipopeptid is present in the fermented liquid of microorganism, output is very low, and complicated component in the fermented liquid, the technology of the lipopeptid that separation and Extraction is purer is comparatively complicated, have in its production cost be about 70～90% to be used in all that middle and lower reaches are extracted and separating technology on, thereby the large-scale application of lipopeptid just is restricted.
Summary of the invention: the present invention is intended to study a kind of lipopeptid biological tensio-active agent that has than low surface tension, improves the content of lipopeptid, is applied in the compost treatment better.
The present invention is achieved through the following technical solutions the foregoing invention purpose.
The lipopeptid biological tensio-active agent is to be produced through fermentation culture by subtilis (Bacillus subtilis), and output reaches 3.5～5g/L.The medium component of producing the subtilis of lipopeptid is: vegetables oil 50～60g/L, NH
4Cl4～6g/L, MgSO
40.15～0.25g/L, CaCl
20.05～0.1g/L, K
2HPO
43.5～4.5g/L, NaH
2PO
46.5～7.5g/L, pH6.0～6.8.The culture condition of fermentor tank is that temperature is 36～38 ℃, and 450～550 rev/mins of mixing speed, air flow are 1.1～1.3vvm.The application of lipopeptid biological tensio-active agent, be with the compost treatment of lipopeptid biological tensio-active agent as domestic refuse, the fermented liquid of fermentation culture through centrifugal remove thalline after, be mixed with 0.01～0.05% lipopeptid solution, with 1～5% uniform mixing of rubbish weight in the domestic refuse of classification.
Description of drawings:
Rubbish interstitial fluid surface tension variations in Fig. 1 compost composting process;
CO in Fig. 2 compost composting process
2Produce rate variation.
The below is described in further detail the present invention.
Bacillus subtilis (Bacillus subtilis) is available from Chinese Typical Representative culture collection center (CCTCC), and preserving number is AB90008. Strain transfer is deposited on the beef extract-peptone slant medium, and medium component is: beef extract 2.0g/L, peptone 5.0g/L, NaCl5.0g/L, agar 20.0g/L, pH7.0.
The seed culture based component is: sucrose 18～22g/L, NH4Cl 4～6g/L，MgSO
4 0.15～0.25g/L，CaCl
2
0.05～0.1/L，K
2HPO
4 3.5～4.5g/L，NaH
2PO
46.5～7.5g/L, dusty yeast 0.5～0.8g/L, pH6.4～6.6. The triangular flask liquid amount is 20%, 36～37 ℃ of cultivation temperature, and shaking speed 180～220rpm, after cultivating 24～36 hours, the inoculum concentration with 6～10% is seeded in the fermentation medium.
Fermentation culture based component: vegetables oil 50～60g/L, NH
4Cl4～6g/L, MgSO
40.15～0.25g/L, CaCl
20.05～0.1g/L, K
2HPO
43.5～4.5g/L, NaH
2PO
46.5～7.5g/L, pH6.0～6.8.The culture condition of fermentor tank is that temperature is 36～38 ℃, and 450～550 rev/mins of mixing speed, air flow are 1.1～1.3vvm.
This bacterial strain has stronger bio-surfactant throughput, simultaneously under the inducing action of hydrophobic nature carbon source vegetables oil, makes this bacterial strain in the logarithmic growth later stage with produce the amount maximum of lipopeptid lag phase, reaches 3.5～5g/L.
Subtilis is through 2～4 days cultivation, fermented liquid through centrifugal (7000～10000rpm) remove thalline, obtain lipopeptid solution, are sufactin through identifying this lipopeptid, and molecular structure (HPD is 8-carboxyl-different pentadecanoyl) is:
All the other are neutral fat, polysaccharide, and surface tension is 27～29mN/m.
With the lipopeptid product that obtains, be mixed with 0.01～0.05% lipopeptid solution, can use by following dual mode, according to the water ratio difference of rubbish, the amount of application of lipopeptid solution is 1～5% of a rubbish weight:
1. lipopeptid can add in mixed stirring device, thus in the process that rubbish mix to stir with the abundant mixing of rubbish, the microenvironment of refuse treatment was promptly improved in the starting stage;
2. in the operational process as need add bio-surfactant, then need cooperate humidity control apparatus.In the water source of sprinkling system, add certain density lipopeptid solution.Along with replenishing of moisture, bio-surfactant just can enter reactor, thereby improves the existence microenvironment of microorganism.
In case tensio-active agent is introduced into the gap of granulated garbage, the hydrophobic side of surfactant molecule will with the organic waste affinity, make molecule be fixed on the granulated garbage surface; Water-wet side is dissolved in the intrapore water of particle.The not same-action of this amphipathic group makes water form stable liquid film on the granulated garbage surface, and the reaction for microorganism of maximum possible provides surperficial environment.Simultaneously, the solubleness of organism in moisture film can be improved in the hydrophobic side of surfactant molecule, thereby quickens microorganism to organic decomposition; In addition, the adding of tensio-active agent also helps the bioavailability that improves hardly degraded organic substance.Measure by experiment and can find, add in the compost reactor of lipopeptid,, make the generation speed of CO2 greater than common compost reactor because microorganism is movable vigorous.After becoming thoroughly decomposed, compost finished product character is better, steady quality.
Embodiment:
1, the preparation of lipopeptid biological tensio-active agent
1) subtilis is seeded to the seed culture medium from slant medium, carries out shake-flask culture, the bottled nutrient solution 100ml of the triangle of 500ml, 37 ℃, 200rpm were cultivated 24 hours;
2) in the automatic fermenter of 5L, pack into the fermention medium of 4L, inoculation subtilis seed culture fluid 100mL, 37 ℃ of temperature, 500 rev/mins of mixing speed, air flow is 1.2vvm, and with HCl and NaOH control pH value 6.5, fermentation time is 76 hours, obtain containing the nutrient solution 3.6L of tensio-active agent, the surface tension of this fermented liquid is 28.5mN/m;
3) fermented liquid centrifugal (8000rpm) is removed thalline, obtain lipopeptid solution, by detecting, lipopeptid content is 4.5g/L;
4), obtain 0.03% lipopeptid solution with 15 times of lipopeptid solution dilutions.Seed culture medium and fermentation culture based component are as follows:
| Seed culture medium | Fermention medium | |
| Glucose | ?20.0g/L | ?- |
| Vegetables oil | ?- | ?55g/L |
| ?NH 4Cl | ?5.00g/L | ?5.00g/L |
| ?MgSO 4 | ?0.20g/L | ?0.20g/L |
| ?CaCl 2 | ?0.08g/L | ?0.08g/L |
| ?K 2HPO 4 | ?4.0g/L | ?4.0g/L |
| ?NaH 2PO 4 | ?6.8g/L | ?6.8g/L |
| Yeast powder | ?0.07g/L | ?0.07g/L |
| ?pH | ?6.5 | ?6.5 |
2, the application in compost
Gather the domestic refuse 20kg in Changsha living quarters, obtain Compostable 8kg through broken, screening, after measured, the water ratio of rubbish is 55%, C/N=25, organic content 60%.Rubbish is packed in the composting device, and the volume of this device is 12L, but load garbage 9L.When packing rubbish into, spray the lipopeptid solution of above-mentioned concentration, the sprinkling amount is 2% of a rubbish weight.
Extract the rubbish interstitial fluid in the composting process, measure surface tension, detect CO in the composting process simultaneously
2Generation speed, the surface tension and the CO of the rubbish interstitial fluid in the composting process
2Generation speed see Fig. 1 and Fig. 2.
By comparing with the compositing system that does not spray lipopeptid solution, can find to spray in the compositing system of lipopeptid solution, the surface tension of rubbish interstitial fluid is lower, and CO
2Generation speed relatively large, compost speed is very fast, has shortened the time of compost.
Claims (3)
1. a lipopeptid biological tensio-active agent is characterized in that this bio-surfactant is to be produced through fermentation culture by subtilis (Bacillus subtilis), and lipopeptid content is 3.5～5g/L.
2. lipopeptid biological tensio-active agent according to claim 1 is characterized in that, the medium component of producing the subtilis of lipopeptid is: vegetables oil 50～60g/L, NH
4Cl4～6g/L, MgSO
40.15～0.25g/L, CaCl
20.05～0.1g/L, K
2HPO
43.5～4.5g/L, NaH
2PO
46.5～7.5g/L, pH6.0～6.8, the culture condition of fermentor tank is that temperature is 36～38 ℃, 450～550 rev/mins of mixing speed, air flow are 1.1～1.3vvm.
3. the application of a lipopeptid biological tensio-active agent as claimed in claim 1, it is characterized in that the compost treatment of lipopeptid biological tensio-active agent as domestic refuse, the fermented liquid of fermentation culture through centrifugal remove thalline after, be mixed with 0.01～0.05% lipopeptid solution, with 1～5% uniform mixing of rubbish weight in the domestic refuse of classification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031180396A CN1184324C (en) | 2003-01-23 | 2003-01-23 | Lipopeptide biosurfactant and its application in making domestic garbage turn to compost |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031180396A CN1184324C (en) | 2003-01-23 | 2003-01-23 | Lipopeptide biosurfactant and its application in making domestic garbage turn to compost |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1431314A true CN1431314A (en) | 2003-07-23 |
| CN1184324C CN1184324C (en) | 2005-01-12 |
Family
ID=4790741
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|---|---|---|---|
| CNB031180396A Expired - Fee Related CN1184324C (en) | 2003-01-23 | 2003-01-23 | Lipopeptide biosurfactant and its application in making domestic garbage turn to compost |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928686A (en) * | 2010-05-20 | 2010-12-29 | 黑龙江大学 | Bacillus subtilis secreting surfactant |
| CN101086006B (en) * | 2007-06-26 | 2011-02-09 | 湖南大学 | Composite type biological surfactant and its production method |
| CN102373258A (en) * | 2011-11-03 | 2012-03-14 | 大庆华理能源生物技术有限公司 | Industrialized preparation method of a lipopeptide biosurfactant |
| CN106119323A (en) * | 2016-07-11 | 2016-11-16 | 珀莱雅化妆品股份有限公司 | A kind of fermentation culture method improving sodium bacillus subtilis lipopeptide yield |
| CN108676837A (en) * | 2018-06-12 | 2018-10-19 | 中林山水（北京）生态科技股份有限公司 | A method of compost is manufactured based on house refuse |
| CN112209762A (en) * | 2019-07-12 | 2021-01-12 | 山东金必来生物科技有限公司 | Macroelement water-soluble fertilizer containing microbial metabolite |
| WO2021183526A1 (en) * | 2020-03-10 | 2021-09-16 | Locus Ip Company, Llc | Compositions for replacing chemical surfactants |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352227A (en) * | 2011-08-08 | 2012-02-15 | 中国海洋石油总公司 | Lipopeptide biosurfactant, preparation method thereof, and application thereof |
-
2003
- 2003-01-23 CN CNB031180396A patent/CN1184324C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101086006B (en) * | 2007-06-26 | 2011-02-09 | 湖南大学 | Composite type biological surfactant and its production method |
| CN101928686A (en) * | 2010-05-20 | 2010-12-29 | 黑龙江大学 | Bacillus subtilis secreting surfactant |
| CN102373258A (en) * | 2011-11-03 | 2012-03-14 | 大庆华理能源生物技术有限公司 | Industrialized preparation method of a lipopeptide biosurfactant |
| CN102373258B (en) * | 2011-11-03 | 2013-11-27 | 大庆华理能源生物技术有限公司 | Industrialized preparation method of lipopeptide biosurfactant |
| CN106119323A (en) * | 2016-07-11 | 2016-11-16 | 珀莱雅化妆品股份有限公司 | A kind of fermentation culture method improving sodium bacillus subtilis lipopeptide yield |
| CN106119323B (en) * | 2016-07-11 | 2019-02-26 | 珀莱雅化妆品股份有限公司 | A kind of fermentation culture method that sodium bacillus subtilis lipopeptide yield can be improved |
| CN108676837A (en) * | 2018-06-12 | 2018-10-19 | 中林山水（北京）生态科技股份有限公司 | A method of compost is manufactured based on house refuse |
| CN112209762A (en) * | 2019-07-12 | 2021-01-12 | 山东金必来生物科技有限公司 | Macroelement water-soluble fertilizer containing microbial metabolite |
| WO2021183526A1 (en) * | 2020-03-10 | 2021-09-16 | Locus Ip Company, Llc | Compositions for replacing chemical surfactants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1184324C (en) | 2005-01-12 |
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