CN1219886C - Composite biosurfactant and its application in compost - Google Patents

Composite biosurfactant and its application in compost Download PDF

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Publication number
CN1219886C
CN1219886C CNB03118040XA CN03118040A CN1219886C CN 1219886 C CN1219886 C CN 1219886C CN B03118040X A CNB03118040X A CN B03118040XA CN 03118040 A CN03118040 A CN 03118040A CN 1219886 C CN1219886 C CN 1219886C
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China
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composite
compost
composite bacteria
active agent
tensio
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CN1431312A (en
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傅海燕
曾光明
黄国和
袁兴中
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Hunan University
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Hunan University
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The present invention relates to a composite biosurfactant generated by composite micro organisms, and the application of the composite biosurfactant. Composite bacteria of the present invention are pseudomonas aeruginosa, bacillus subtilis, a small quantity of bacillus licheniformis and ainetabactera, etc. The composite bacteria are fermented and cultivated in a culture medium which uses olive oil as a carbon source. An additive agent of the high-concentration composite biosurfactant is generated. The additive agent of the composite biosurfactant and the thalli of the additive agent are added into the raw material of common living garbage organic compost. The interfacial tension of organic particles in the raw material of compost is decreased in order to increase the affinity of various kinds of compost thalli and organic particles. An interfacial liquid film suitable for micro organism growth is provided in order to accelerate the assimilation of the micro organisms on the organic particles. Thus, the composting time is shortened, and the composting efficiency is increased.

Description

Compound bio tensio-active agent and the application in compost thereof
Technical field: the present invention relates to a kind of tensio-active agent, be specifically related to the bio-surfactant of microorganisms.
Background technology: an important means of China's garbage treatment is a compost at present, just biodegradable organism in the domestic refuse is partly carried out biological decomposition, and makes it stabilization and innoxious.In composting process, microorganism is to carry out in one deck liquid film on granulated garbage surface in the gap organic decomposition.Therefore, the physicochemical condition in granulated garbage gap has very significant effects to the compost result in the reaction system.In view of the granulated garbage gap is the ecological microenvironment of microbial reaction, how to improve this microenvironment, make it more to help the decomposition reaction of microorganism to organic waste, be one of key that improves the garbage compost processing efficiency.
The traditional method of improving the medium physicochemical condition has: the C/N ratio of control compositing system, temperature, humidity, oxygen, voidage etc.The application of these means in composting process is necessary, but the purport of these methods all is to improve mass transfer or help microorganism growth, and in the interaction of improvement microorganism and medium and directly promote microorganism that the decomposition course of hardly degraded organic substance is had little scope for one's talents.Some addings with material (as tensio-active agent) of special construction will be the useful trials that improves the ecological microenvironment of microbial reaction, yet existing " conventional surfactant " is toxic to the microorganism in the rubbish; " green surfactant " then preferentially degraded by microorganism too easily, thereby loses its meaning as tensio-active agent.Therefore, developing did not a kind ofly both have toxicity to microorganism in the rubbish, and the tensio-active agent that can be difficult for preferentially being degraded in rubbish then is to need the problem that solves again.
Summary of the invention: the present invention is intended to develop a kind of compound bio tensio-active agent, the organic compost feed particles Interfacial Liquid Membranes in the rubbish of making the life better, the reaction for microorganism of maximum possible provides good surperficial environment, thereby quickens microorganism to organic decomposition.
The present invention realizes the foregoing invention purpose by the following technical solutions.
The compound bio tensio-active agent is the organic granular interface modifying agent that contains rhamnolipid, lipopeptid and a spot of lipid acid that composite bacteria such as the Pseudomonas aeruginosa that obtains through enrichment culture from the soil that is rich in soil ulmin, subtilis and a small amount of Bacillus licheniformis, acinetobacter calcoaceticus produce through fermentation culture, and its surface tension value is 33～36mN/m.The composite bacteria fermentation culture that produces bio-surfactant is 35～38 ℃ of temperature, 500～700 rev/mins of stirring velocitys, and air flow is 1.2～1.6vvm, carries out under the condition of pH value 6.5～7.0, and inoculum size is 5～8%, and substratum is: Na 2HPO 40.25～0.30g/L, KH 2PO 41.0～1.2g/L, NH 4NO 32.8～3.2g/L, K 2SO 40.35～0.4g/L, MgSO 47H 2O 0.13～0.16g/L, CaCl 22H 2O 0.02～0.03g/L, FeSO 47H 2O 0.035～0.04g/L, NaCl 0.08～0.12g/L, yeast extract paste 0.40～0.60g/L, trace element solution 0.8～1.2mL/L, sweet oil 8～12g/L.
The application of compound bio tensio-active agent, be with the composting process of this compound bio tensio-active agent as domestic refuse, with the composite bacteria fermented liquid and the pending domestic refuse uniform mixing of the compound bio tensio-active agent that produces, the consumption of composite bacteria fermented liquid is 0.3～0.8% of a rubbish weight.
Description of drawings:
Fig. 1 tests sample and contrasts sample raw material gross weight change curve in time;
Fig. 2 tests sample and contrasts the content of sample C in dry-matter variation diagram in time;
The below is described in further detail the present invention.
The complex microorganism bacterial classification is to contain from the mountain that separation and purification obtains the abundant topsoil sample of humus, should be respectively below 20 centimetre, and 10 centimeters and topsoil sampling. Also can near oil factory, grain and oil factory, dining room, be taken a sample the grease degree of depth contaminated soil.
The soil-like that collects is made the soil dilution, then be inoculated in the optimum enriched medium that obtains by orthogonal experiment and cultivate. This enriched medium has suitable surfactant and produces bacteria growing, and suppresses the function of common growth. The enrichment culture based component is mainly indispensable element, trace element, the minute quantity yeast extract of various fungus grown. Its main component sees Table 1.
Table 1 enriched medium component list (unit: g/L)
    Na 2HPO 4     KH 2PO 4     NH 4NO 3     K 2SO 4     MgSO 4·7H 2O     0.25～0.30     1.0～1.2     2.8～3.2     0.35～0.4     0.13～0.16   CaCl 2·2H 2O     0.02～0.03   FeSO 4·7H 2O 0.035～0.04 NaCl 0.08～0.12 yeast extract 0.40～0.60 pH 6.3～6.7
The add-on of trace element solution is 1mL/L in the enrichment medium, and main component sees Table 2.
Table 2 trace element solution component list (unit: μ g/L)
KI CoCl 2·6H 2O Na 2MoO 4·2H 2O CuSO 4·5H 2O 0.83 0.048 0.048 0.125 H 3PO 4 MnSO 4·4H 2O ZnCl 2 0.01 0.223 0.287
After placing constant temperature air bath concussion incubator to cultivate 1～2 day the enrichment medium of having inoculated soil-like, there is obvious thalline to produce, be inoculated into enrichment medium then and add that (add-on of sweet oil is 8～12g/L) in the minimum medium of sweet oil, place constant temperature air bath concussion incubator to continue to cultivate, and sampling in per 24 hours is surveyed surface tension value with automatic interfacial tensimeter.The culture that surface tension value is lower than 36mN/m is defined as having the composite bacteria than Johnson ﹠ Johnson's thing tensio-active agent throughput.
Culture condition is: 37 ℃ of temperature, 220 rev/mins of rotating speeds, triangular flask liquid amount are 20%.
The agar plate line partition method that above-mentioned fermented liquid is used always is carried out the initial gross separation purifying.The main component of agar plate sees Table 3.
Table 3 Agar Plating main component table (unit: g)
Peptone 15 Agar powder 20
Extractum carnis 5 Distilled water 1000
NaCl 5 pH 7.2
Bacterial classification behind the preliminary purification carries out the purification of above-mentioned steps again, obtain producing the stronger composite bacteria of bio-surfactant ability at last, composite bacteria is mainly Pseudomonas aeruginosa (Pseudomonas aeruginosa), subtilis (Bacillussubtilis) and a small amount of Bacillus licheniformis (Bacillus licheniformis), acinetobacter calcoaceticus (Acinetobacter sp.) etc.The surface tension value of the fermented liquid of this composite bacteria is 33～36mN/m.By analysis, mainly contain in the fermented liquid rhamnolipid (4.5～6.3g/L), lipopeptid (1.1～1.9g/L) and a spot of lipid acid (0.1～0.3g/L).After measured, the compound bio surfactant concentrations is 28～35 times of micelle-forming concentration.
The composite bacteria of separating is inoculated into 4 ℃ of preservations, standby in the slant medium.The composition of slant medium is: extractum carnis, 2.0g/L; Peptone, 5.0g/L; NaCl, 5.0g/L; Agar, 20.0g/L; PH7.0.
The seed liquor that the composite bacteria of preserving is cultivated is inoculated in carries out large scale culturing in the fermentor tank, inoculum size is 5%～8%, the cultivation of seed liquor and fermented liquid is the minimum medium that enrichment medium adds sweet oil, temperature is 36～38 ℃, 500～700 rev/mins of mixing speed, air flow is 1.2～1.6vvm, and control pH value is 6.5～7.0, and fermentation time is 40～56 hours.
The fermented liquid of large scale culturing is joined in the pending rubbish, make its in the mixing process of rubbish with the abundant mixing of rubbish, the amount of application of fermented liquid is 0.3～0.8% of a rubbish weight.Through adding the compost of compound bio tensio-active agent, can reduce the organic granular interfacial tension in the composting material, the affinity of thalline and organic granular provides the Interfacial Liquid Membranes that is fit to microorganism growth in the increase compost.Improve organic degradation rate, and the bioavailability that improves the compost product.
Embodiment:
1. the composite bacteria fermented liquid is produced: will be inoculated in the fermentor tank through the composite bacteria liquid that seed liquor is cultivated and cultivate, medium component sees Table 4:
Table 4 fermentation culture based component (g/L)
Na 2HPO 4 KH 2PO 4 NH 4NO 3 NaCl K 2SO 4 MgSO 4.7H 2O 0.28 1.14 3.00 0.10 0.36 0.154 CaCl 2·2H 2O 0.023 FeSO 4·7H 2O 0.038 yeast extract paste 0.50 trace element solution 1mL sweet oil 10 pH 6.8
2. the fermented liquid that contains the compound bio tensio-active agent that large scale culturing is obtained is applied in the compost of domestic refuse.General domestic refuse one quasi-representative organic composition sees Table 5.
Table 5. pilot experiment material composition table
Composition Rice grain Green vegetables Fruit Potato Meat
Content (%) 33.7 25.3 16.9 16.9 7.2
C/N 36.14 34.79 36.92 15.28 5.69
5 kilograms raw materials are broken into the particle of particle diameter less than 1.0cm, mix the back with compound bio tensio-active agent fermented liquid 40ml and add reactor.In the reaction process, reactor keeps 50 ℃ of system temperatures by the mode of heating in water bath, and the reaction times is 7 days.Design contrast sample (do not add bio-surfactant, other condition is identical) experiment simultaneously.By measuring two kinds of surface tension of extracting samples, total C and total N, ash content, water ratio etc., the research bio-surfactant is to the effect of compost.
Find that from result of study the total carbon degradation rate in raw material gross weight and the raw material has largely to be accelerated, raw material gross weight and the C content in dry-matter sees Table 6 and Fig. 1 and Fig. 2.
The degraded information slip of table 6. experiment sample and contrast sample raw material
Time (my god) Raw material gross weight (g) experiment sample contrast sample Content (%) the experiment sample contrast sample of C in dry-matter
0 1 2 3 4 5 6 7 4150.0 4150.0 3585.7 3722.8 3323.5 3512.4 3117.4 3364.0 3027.9 3176.4 3011.9 3056.6 3005.7 3034.9 3003.8 3015.0 46.61 46.61 41.45 42.79 40.47 41.61 40.18 40.89 40.10 40.31 40.05 40.22 40.03 40.11 40.03 40.07
Above statement of facts, the adding of bio-surfactant has certain promoter action to the organic compost process of general domestic refuse, can improve degradation rate to a certain extent, and itself can be degraded.This has proved absolutely that bio-surfactant has the potentiality that are applied to the compost suitability for industrialized production as a kind of novel surfactant.

Claims (2)

1. compound bio tensio-active agent, it is characterized in that this bio-surfactant is the Pseudomonas aeruginosa that obtains through enrichment culture from the soil that is rich in soil ulmin, subtilis and a small amount of Bacillus licheniformis, the composite bacteria of acinetobacter calcoaceticus is through the rhamnolipid that contains of fermentation culture generation, lipopeptid, and the organic granular interface modifying agent of a spot of lipid acid, its surface tension value is 33～36mN/m, the content of rhamnolipid is 4.5～6.3g/L, the content of lipopeptid is 1.1～1.9g/L, the content of lipid acid is 0.1～0.3g/L, the composite bacteria fermentation culture that produces bio-surfactant is 35～38 ℃ of temperature, 500～700 rev/mins of stirring velocitys, air flow is 1.2～1.6vvm, carry out under the condition of pH value 6.5～7.0, inoculum size is 5～8%, and substratum is: Na 2HPO 40.25～0.30g/L, KH 2PO 41.0～1.2g/L, NH 4NO 32.8～3.2g/L, K 2SO 40.35～0.4g/L, MgSO 47H 2O 0.13～0.16g/L, CaCl 22H 2O0.02～0.03g/L, FeSO 47H 2O 0.035～0.04g/L, NaCl 0.08～0.12g/L, yeast extract paste 0.40～0.60g/L, trace element solution 0.8～1.2mL/L, sweet oil 8～12g/L, the composition of trace element solution are KI0.83mg/L, CoCl 26H 2O 0.048mg/L, Na 2MoO 42H 2O 0.048mg/L, CuSO 45H 2O 0.125mg/L, H 3PO 40.01mg/L, MnSO 44H 2O 0.22mg/L, ZnCl 20.287mg/L.
2. the application of a compound bio tensio-active agent as claimed in claim 1, it is characterized in that the composting process of this compound bio tensio-active agent as domestic refuse, with the composite bacteria fermented liquid and the pending domestic refuse uniform mixing of the compound bio tensio-active agent that produces, the consumption of composite bacteria fermented liquid is 0.3～0.8% of a rubbish weight.
CNB03118040XA 2003-01-23 2003-01-23 Composite biosurfactant and its application in compost Expired - Fee Related CN1219886C (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8217004B2 (en) * 2005-03-15 2012-07-10 Adelaide Research And Innovation Pty. Ltd. Sequestering agent for micronutrient fertilisers
CN103070167B (en) * 2010-03-30 2014-11-26 湖州紫金生物科技有限公司 Application of rhamnolipid as additive
CN103865819B (en) * 2012-12-07 2018-10-26 丰益(上海)生物技术研发中心有限公司 One plant of pseudomonad for producing lipopeptid class surface-active and purposes
CN103848701B (en) * 2014-03-31 2015-11-11 北京林业大学 A kind of preparation method of soilless plant cultivation matrix and the matrix prepared by the method
CN107497364A (en) * 2016-06-14 2017-12-22 洛阳华清天木生物科技有限公司 A kind of composite biosurfactant and its application
CH712859A2 (en) 2016-08-29 2018-03-15 Remo Richli Washing, care and cleaning preparations containing polyoxyalkylene carboxylate and glycolipid biosurfactant.
CH712858A2 (en) 2016-08-29 2018-03-15 Remo Richli Mild preparations containing alkoxylated fatty acid amides and glycolipid biosurfactants.
CH712860A2 (en) 2016-08-29 2018-03-15 Remo Richli Agents with alkoxylated fatty acid amides and glycolipid biosurfactants.
CN106495871B (en) * 2016-11-01 2019-11-26 西北农林科技大学 A kind of method of macrolide resistant gene and intI1 abundance in reduction Chicken Manure Compost
CN108753840A (en) * 2018-06-12 2018-11-06 中林山水（北京）生态科技股份有限公司 A kind of preparation method of composite biosurfactant
CN108676837A (en) * 2018-06-12 2018-10-19 中林山水（北京）生态科技股份有限公司 A method of compost is manufactured based on house refuse
CH720165A2 (en) 2022-10-26 2024-04-30 Chemtek Ug Compositions with N-acylglycamines

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