CN1422957A - Method for preparing bioactive micro peptide of plant protein - Google Patents
Method for preparing bioactive micro peptide of plant protein Download PDFInfo
- Publication number
- CN1422957A CN1422957A CN 01140321 CN01140321A CN1422957A CN 1422957 A CN1422957 A CN 1422957A CN 01140321 CN01140321 CN 01140321 CN 01140321 A CN01140321 A CN 01140321A CN 1422957 A CN1422957 A CN 1422957A
- Authority
- CN
- China
- Prior art keywords
- plant protein
- condition
- under
- liquid
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention is a method for producing plant albument biological active peptide by yeasting. The yeasting substrate contains plant albumen material under condition of the existence of seeds yeasting agent. The mentioned seeds yeasting agent is ferment or microbe fungus or the mikxture of them. The mentioned ferment is selected from one or more of glycolytic ferment, amylase, proteinase, semi-fibrin ferment and fibrin ferment. The microbe fungus is selected from one or more of yellow espergillosis, black aspergillosis, sprout bacilli, gross mould, root mould, yeast and plant lactobacillus.
Description
Technical field
The present invention relates to make the method for little peptide, particularly relate to a kind of method of making bioactive micro peptide of plant protein with the fermentation means with the fermentation means.
Background technology
Little peptide is the essential nutrient substance of animal and human.Discovering of physiology of nutrition in recent years, little peptide plays an important role in amino acid digestion, absorption and Animal nutrition metabolism as proteinic main digestion product.Little peptide is that the little peptide absorption rate specific ionization amino acid whose speed of digesting and assimilating form one of, particularly 2-10 amino acid composition of protein in enteron aisle is also fast.This has proposed challenge for people and livestock and poultry gal4 amino acid nutrition " cylinder " principle, has proposed new approaches for setting up the assessment of nutritional value new system and developing the novel protein nutritional additive.
Natural little peptide and the little peptide of proteolysis have multiple biological action, can regulate the vital movement of body.Exogenous bioactive peptide is potential in protein, the active centre sequence of they and endogenous bioactive peptide is same or similar, in digestive process, discharge, by directly combining with intestinal receptor, the physiological regulatory action or the complete absorption that participate in body enter blood circulation, enter target tissue, performance and endogenous bioactive peptide identical functions.Little peptide material generally directly is not present in food or the feed, but is present in many food proteins sequences, can discharge bioactive peptide through enzymolysis.
Bioactive micro peptide of plant protein is to be basic raw material with leguminous forage powder, various grouts, various bean powder, by modern microbial fermentation technology vegetable-protein is converted into little peptide.Little peptide absorbs soon in vivo, utilization ratio is high.Bioactive micro peptide of plant protein outside easy the absorption, also has functions such as trace element absorption such as the growth of promotion, adjusting digestion and absorption function, anti-stress, adjusting immunocompetence, promotion calcium phosphorus except nutritious.
Though the physiological function and the mechanism of action to various bioactive micro peptides have had more research, to its Research on Manufacturing Method seldom.A kind of method of making milk protein active peptide is in the prior art, is raw material with fresh milk or skim-milk, with the short lactobacillus bacterial classification casein in milk or the skim-milk is changed into bioactive peptide, but the newborn bioactive peptide cost of producing with this method is very high.The another kind of method that obtains little peptide is to adopt chemistry and biological synthesis process, is raw material with amino acid, and chemistry or biological process synthesize little peptide, and the amino acid The Nomenclature Composition and Structure of Complexes that the little peptide advantage of producing with this method is little peptide determines that shortcoming is the cost height.Also has a kind of method of making active peptide of soybean protein, be with soybean protein isolate, soyflour, soybean meal, any in the soya-bean cake is raw material, raw material carries out pre-treatment earlier, adding water move to material concentration is 10-12%, and adjust pH is 6.5-7.0, then at high temperature, carry out sterilising treatment under the high pressure, be cooled to 45 ℃, the 0.4-0.6% that presses solid substance inserts aspergillus oryzae, stirs, and carries out 20-30 hour fermentative processing, maintain the temperature at 45 ℃ ± 2 ℃ during fermentation, and constantly stir, fermentation ends is carried out high-temperature inactivation, filtering separation then, the supernatant liquor reconcentration is to 40BX, and drying is made product.The reacting balance of this method, than being easier to control, the foreign matter content in its finished product is low, peculiar smell such as no bitterness, and also its production cost is lower.But this method adopts aspergillus oryzae list strain fermentation, and zymogram is narrower, can't be used for the daily ration of the suitability for industrialized production mankind and livestock and poultry.
Summary of the invention
The purpose of this invention is to provide a kind of low cost, make the method for bioactive micro peptide of plant protein easily.
For achieving the above object, the present invention takes following technical scheme: a kind of method of making bioactive micro peptide of plant protein is under the condition that the seed fermentation agent exists, and the fermention medium that contains plant protein material is fermented; Described seed fermentation agent is the mixture of enzyme or microbial strains or enzyme and microbial strains; Described enzyme is selected from one or more in saccharifying enzyme, amylase, proteolytic enzyme, hemicellulase and the cellulase; Described microbial strains is selected from one or more in aspergillus oryzae, aspergillus niger, genus bacillus, Mucor, head mold, yeast and the plant lactobacillus.
When the seed fermentation agent was microbial strains or the mixture of enzyme and microbial strains, the microbial strains in the described seed fermentation agent was to cultivate the bacterial classification that obtains through level liquid at least, and its preparation method is:
1) slant strains: use the malt extract medium of total sugar degree 10-20%, add 2% agar, streak inoculation, under 30-35 ℃ of condition, shaking table was cultivated 20-24 hour, preserved in 4 ℃ of refrigerators;
2) liquid spawn and level liquid are cultivated bacterial classification: use the malt extract medium of total sugar degree 10-20%, the inoculum size of seed liquor is 5-20%, cultivated 20-24 hour at 30-35 ℃ of shaking table, or under 30-35 ℃ of condition, static cultivation 24-48 hour;
3) secondary liquid culture bacterial classification: use sugar degree 10-20% amylum hydrolysate of the sugar liquid, the bacterial classification that the inoculation level liquid is cultivated, inoculum size is 5-20%, cultivated 20-24 hour at 30-35 ℃ of shaking table, or under 30-35 ℃ of condition, static cultivation 24-48 hour;
4) three grades of liquid culture bacterial classifications: use the amylum hydrolysate of the sugar liquid of sugar degree 10-20%, the bacterial classification of inoculation secondary liquid culture, inoculum size is 5-20%, cultivated 20-24 hour at 30-35 ℃ of shaking table, or under 30-35 ℃ of condition, static cultivation 24-48 hour.
5) fermentor tank or vat liquid culture bacterial classification: use amylum hydrolysate of the sugar liquid, sugar degree 10-20%, the bacterial classification of three grades of liquid culture of inoculation, the inoculum size of seed liquor is 5-20%, under 25-35 ℃ condition, and stir culture 20-24 hour, or under 25-35 ℃ condition, static cultivation 24-48.
Under the condition that the seed fermentation agent exists, the fermention medium that contains plant protein material is fermented, can adopt the method for liquid fermenting, also can adopt the method for solid fermentation.During liquid fermenting, the prescription of fermention medium is (ratio of weight and number):
Plant protein material 30-85
Root of Cornu Cervi Pantotrichum powder 5-10
Wheat bran 5-30
Corn cob 0-30
Semen Maydis powder 0-20
Wheat flour 0-10
Urea or ammonium sulfate 0-0.5
Add water, the gross weight of above-mentioned raw materials: water=1: 1.5-8; The inoculum size of seed fermentation agent is 5-30%; PH6-7; Under 35-50 ℃ the condition, ventilation O
2=25-30%.In order to make the better effects if of fermentation, can also be added with in inorganic salt, VITAMIN, trace element or the calcium preparation one or more in the described substratum.Tunning obtains product after filter press, drying.
During solid fermentation, the prescription of fermention medium is (ratio of weight and number):
Plant protein material 30-85
Root of Cornu Cervi Pantotrichum powder 5-10
Wheat bran 5-30
Corn cob 0-30
Semen Maydis powder 0-20
Wheat flour 0-10
Urea or ammonium sulfate 0-0.5
Add water, the gross weight of above-mentioned raw materials: water=1: 0.5-1.5, the inoculum size of seed fermentation agent is 5-30%; Under 15-50 ℃ the condition, static cultivation 1-4 days.In order to make the better effects if of fermentation, can also be added with in inorganic salt, VITAMIN, trace element or the calcium preparation one or more in the described substratum.Tunning nature airing or oven drying at low temperature to moisture below 65 ℃ are lower than 12%, pulverize and obtain product.
Among the present invention, described plant protein material is one or more in leguminous forage powder, garden sorrel, grouts, Zein powder or the bean powder.Described grouts are one or more in soybean cake dregs, cotton cake dregs, dregs of rapeseed cake, peanut dregs, Sunflower Receptacle grouts, flaxseed grouts or the sesame cake meal.
With the sodium chloride solution extraction of tunning of the present invention, record the 30-80% that soluble substance accounts for dry substance mixture with 2-6 times of volume 0.1-0.15%.Record with Kjeldahl determination, crude protein content accounts for the 40-60% of dry substance mixture.Record amino acid and little peptide attitude nitrogen content accounts for total nitrogen content 30-70% with the NaOH volumetry.
The present invention has the following advantages owing to adopted technique scheme: 1, low, the less investment of production cost, instant effect, high efficiency.2, practical, easy and simple to handle, be easy to promote.3, fermentation period is short, the production efficiency height.4, the bioactive micro peptide of plant protein of being produced has multiple biological activity, can promote growth of animal, can also eliminate the antinutritional factor in the vegetable-protein simultaneously, improves food utilization efficiency.5, the more former nutrient protein of the bioactive micro peptide of plant protein of Huo Deing is worth and improves, and is easy to digest and assimilate, and biological function obviously improves, each seed amino acid of while, and the content of VITAMIN also obviously improves.6, according to various livestock and poultry, fish and people's nutritive property, can produce dissimilar bioactive micro peptide of plant protein, satisfy many-sided demand.7, the bioactive micro peptide of plant protein of producing with the inventive method can reduce the indispensable amino acid consumption, substitutes animal protein, solves the under-supply problem of high-quality protein feed, alleviates disparities between supply and demand, has remarkable social benefit and economic benefit.
The bioactive micro peptide of plant protein that utilizes the inventive method to produce can be used for various domestic animal daily rations, human daily ration and invigorant, fish daily ration, shrimp crab terrapin daily ration and other cultivated animals daily ration.
Concrete what bacterial classification and what enzyme of using can be selected suitable bacterial classification and enzyme according to the practical situation of coming grown places such as source category, production cost of vegetable-protein in the seed fermentation agent.
The present invention will be further described below in conjunction with specific embodiment.
Embodiment
Embodiment 1, produce bioactive micro peptide of plant protein with single culture
1, preparation seed fermentation agent:
Select aspergillus niger strain for use, carry out following operation:
1) slant strains: use the malt extract medium of total sugar degree 20%, streak inoculation, under 30 ℃ of conditions, shaking table was cultivated 24 hours, preserved in 4 ℃ of refrigerators;
2) liquid spawn and level liquid are cultivated bacterial classification: use the amylum hydrolysate of the sugar liquid of total sugar degree 20%, and the inoculation slant strains, inoculum size is 20%, shaking table was cultivated 24 hours under 30 ℃ of conditions;
3) secondary liquid culture bacterial classification: use the amylum hydrolysate of the sugar liquid of sugar degree 20%, the inoculation level liquid is cultivated bacterial classification, and inoculum size is 20%, and shaking table was cultivated 20 hours under 30 ℃ of conditions;
4) three grades of liquid culture bacterial classifications: use the amylum hydrolysate of the sugar liquid of total sugar degree 20%, inoculation secondary liquid culture bacterial classification, inoculum size is 20%, shaking table was cultivated 24 hours under 30 ℃ of conditions;
5) fermentor tank liquid culture bacterial classification: use the amylum hydrolysate of the sugar liquid of sugar degree 10%, inoculate three grades of liquid culture bacterial classifications, inoculum size is 5%, under 35 ℃ of conditions, and stir culture 24 hours.
2, with the seed fermentation agent fermention medium that contains plant protein material is carried out solid fermentation
The fermentative medium formula that uses is counted with parts by weight: soybean cake: 50 parts; Rapeseed cake: 20; 5 parts in root of Cornu Cervi Pantotrichum powder; 25 parts in wheat bran; The water content of above substratum is controlled at 80%, keeps nature pH value.After substratum stirs, profit water half an hour, go into pot and steam material, normal pressure 1.5 hours.Sieve after taking the dish out of the pot and be cooled to 40 ℃.The seed fermentation agent that adds above-mentioned fermentor cultivation gained, inoculum size are under 20%, 45 ℃ of condition, static cultivation 3 days.
3, oven dry and pulverizing obtain product
With the solid fermentation product of cultivating, natural airing to moisture is lower than 12%, pulverizes then and makes the bioactive micro peptide of plant protein product.
4, the compositional analysis of tunning: tunning records soluble substance and accounts for 65% of dry substance mixture with the sodium chloride solution extraction of 6 times of volumes 0.1%, and crude protein content accounts for 45% of dry-matter, and amino acid and little peptide attitude nitrogen content account for total nitrogen content 70%.
Embodiment 2, produce bioactive micro peptide of plant protein with the mixture of composite bacteria and prozyme
1, preparation seed fermentation agent:
1) mixture of selecting following microbial strains for use is as the bacterium source, and the method for making of its fermented liquid is with embodiment 1:
A) aspergillus niger; B) Mucor; C) head mold
2) select for use following enzyme as the enzyme source:
A) saccharifying enzyme; B) hemicellulase; C) proteolytic enzyme;
3) with 1) in bacterium source and 2) in the enzyme source mix, ratio is 1: 2.
2, with the seed fermentation agent fermention medium that contains plant protein material is carried out solid fermentation
1) medium preparation: the fermentative medium formula of use is counted with parts by weight: peanut dregs: 68.5 parts; 5 parts in root of Cornu Cervi Pantotrichum powder; 25 parts in wheat bran; 1.5 parts in lime; 0.1% 50% derosal stirs, and the water content of above substratum is controlled at 100%, keeps nature pH value.
2) the bacterium bed is made: spread the plastic film of sterilizing with 0.2% potassium permanganate solution on the bacterium bed earlier, plastic film spreads a little seed fermentation agent earlier than 2.5 times of bacterium bedsides on the film, repave the material of half, spread the agent of one deck seed fermentation on the charge level again, spread second half material then, spread the agent of one deck seed fermentation on the charge level, the inoculum size of seed fermentation agent is 30%, the thick 15cm of bed material, after planting, cover charge level with plastic film again, in order to heat and moisture preserving with bed surface compacting slightly.Under 35 ℃ the condition, static cultivation 4 days.
3, oven dry and pulverizing obtain product
With the solid fermentation product of cultivating, oven drying at low temperature to moisture below 65 ℃ is lower than 12%, pulverizes then and makes the bioactive micro peptide of plant protein product.
4, the compositional analysis of tunning: tunning records soluble substance and accounts for 80% of dry substance mixture with the sodium chloride solution extraction of 6 times of volumes 0.15%, and crude protein content accounts for 49% of dry-matter, and amino acid and little peptide attitude nitrogen content account for total nitrogen content 70%.
Embodiment 3, produce bioactive micro peptide of plant protein with composite bacteria
1, preparation seed fermentation agent:
1) mixture of selecting following microbial strains for use is as the bacterium source, and the method for making of its fermented liquid is with embodiment 1:
A) aspergillus niger; B) aspergillus oryzae
2, with the seed fermentation agent fermention medium that contains plant protein material is carried out solid fermentation
1) medium preparation: the fermentative medium formula of use is counted with parts by weight: Zein powder: 70 parts; Root of Cornu Cervi Pantotrichum powder: 5 parts; Wheat bran: 25 parts; Flour: 5 parts, substratum stirs, and water content is controlled at 80%, keeps nature pH value.Profit water half an hour, go into pot and steam material, 0.11Mpa 30 minutes.Sieve after taking the dish out of the pot and be cooled to 35 ℃.
2) the fermentation charging tray is made: insert 30% seed fermentation agent in the substratum, first trick layer culture is 13 hours on the fermentation charging tray, expects thick 13cm, and temperature 30-32 ℃, moisture 50%.Cover charge level with plastic film then, 29 ℃ of heat and moisture preservings, static cultivation was divided in whole card after 12 hours, expected thick 1.5cm, kept 50 ℃ of temperature, and stirring after 24 hours was cultivated 36 hours for 30 ℃.
3, oven dry and pulverizing obtain product
With the solid fermentation product of cultivating, oven drying at low temperature to moisture below 75 ℃ is lower than 12%, pulverizes then and makes the bioactive micro peptide of plant protein product.
4, the compositional analysis of tunning: tunning records soluble substance and accounts for 75% of dry substance mixture with the sodium chloride solution extraction of 6 times of volumes 0.15%, and crude protein content accounts for 45% of dry-matter, and amino acid and little peptide attitude nitrogen content account for total nitrogen content 65%.
Embodiment 4, produce bioactive micro peptide of plant protein with composite bacteria
1, preparation seed fermentation agent:
1) mixture of selecting following microbial strains for use is as the bacterium source, and the method for making of its fermented liquid is with embodiment 1:
A) aspergillus niger; B) aspergillus oryzae; C) yeast
2, with the seed fermentation agent fermention medium that contains plant protein material is carried out liquid fermenting
Medium preparation: the fermentative medium formula of use is counted with parts by weight: the leguminous forage powder: 70 parts; Root of Cornu Cervi Pantotrichum powder: 5 parts; Wheat bran: 5 parts; Flour: 5 parts, leguminous forage powder profit water half an hour, go into pot and steam material, following 2 hours of normal pressure, admix root of Cornu Cervi Pantotrichum powder, wheat bran and flour after taking the dish out of the pot, add the water of 5 times of raw material gross weights, turn evenly, ventilating is cooled to 40 ℃, insert the seed fermentation agent, inoculum size is 10%, turns evenly, goes into the fermentation vat bed.The thick 25cm of bed material, aeration-cooling, in order to heat and moisture preserving, under 50 ℃ the condition, static cultivation 48 hours, during stirring 2 times.
3, the liquid fermentation production of cultivating is obtained product through filter press, oven dry.
4, the compositional analysis of tunning: tunning records soluble substance and accounts for 72% of dry substance mixture with the sodium chloride solution extraction of 6 times of volumes 0.1%, and crude protein content accounts for 45% of dry-matter, and amino acid and little peptide attitude nitrogen content account for total nitrogen content 65%.
Embodiment 5, produce bioactive micro peptide of plant protein with single enzyme
1, preparation seed fermentation agent:
Select for use proteolytic enzyme as the enzyme source:
2, with the seed fermentation agent fermention medium that contains plant protein material is carried out solid fermentation
1) medium preparation: the fermentative medium formula of use is counted with parts by weight: garden sorrel: 85 parts; Root of Cornu Cervi Pantotrichum powder: 5 parts; Wheat bran: 12.9 parts; Lime: 1 part; CuSO
40.1%; 0.1% 50% derosal; 1 part of seed fermentation agent; The water content of above substratum is controlled at 80%, keeps nature pH value.
2) the fermentation bed is made: spread the plastic film of sterilizing with 0.2% potassium permanganate solution earlier on the fermentation bed, add the substratum that stirs on the film.The thick 30cm of bed material after planting with bed surface compacting slightly, covers charge level with plastic film, in order to heat and moisture preserving again.Under 40 ℃ of conditions, static cultivation 1 day.
3, oven dry and pulverizing obtain product
With the solid fermentation product of cultivating, oven drying at low temperature to moisture below 65 ℃ is lower than 12%, pulverizes then and makes the bioactive micro peptide of plant protein product.
4, the compositional analysis of tunning: tunning records soluble substance and accounts for 70% of dry substance mixture with the sodium chloride solution extraction of 6 times of volumes 0.15%, and crude protein content accounts for 40% of dry-matter, and amino acid and little peptide attitude nitrogen content account for total nitrogen content 70%.
Embodiment 6, produce bioactive micro peptide of plant protein with prozyme
1, preparation seed fermentation agent:
The mixture of selecting following enzyme for use is as the enzyme source:
A) saccharifying enzyme; B) hemicellulase; C) proteolytic enzyme; D) amylase
2, with the seed fermentation agent fermention medium that contains plant protein material is carried out solid fermentation
1) medium preparation: the fermentative medium formula of use is counted with parts by weight: Zein powder: 85 parts; Root of Cornu Cervi Pantotrichum powder: 5 parts; Wheat bran: 12.9 parts; Lime: 1 part; CuSO
40.1%; 0.1% 50% derosal; 1 part of seed fermentation agent stirs, and water content is controlled at 80%, keeps nature pH value.
2) the fermentation bed is made: spread the plastic film of sterilizing with 0.2% potassium permanganate solution earlier on the fermentation bed, add the substratum that stirs on the film.The thick 30cm of bed material after planting with bed surface compacting slightly, covers charge level with plastic film again, in order to heat and moisture preserving, and under 40 ℃ of conditions, static cultivation 1 day.
3, oven dry and pulverizing obtain product
With the solid fermentation product of cultivating, oven drying at low temperature to moisture below 65 ℃ is lower than 12%, pulverizes then and makes the bioactive micro peptide of plant protein product.
4, the compositional analysis of tunning: tunning records soluble substance and accounts for 80% of dry substance mixture with the sodium chloride solution extraction of 6 times of volumes 0.15%, and crude protein content accounts for 40% of dry-matter, and amino acid and little peptide attitude nitrogen content account for total nitrogen content 80%.
Claims (10)
1, a kind of method of making bioactive micro peptide of plant protein is under the condition that the seed fermentation agent exists, and the fermention medium that contains plant protein material is fermented; Described seed fermentation agent is the mixture of enzyme or microbial strains or enzyme and microbial strains; Described enzyme is selected from one or more in saccharifying enzyme, amylase, proteolytic enzyme, hemicellulase and the cellulase; Described microbial strains is selected from one or more in aspergillus oryzae, aspergillus niger, genus bacillus, Mucor, head mold, yeast and the plant lactobacillus.
2, a kind of method of making bioactive micro peptide of plant protein according to claim 1 is characterized in that: the microbial strains in the described seed fermentation agent is to cultivate the bacterial classification that obtains through level liquid at least, and its preparation method is:
1) slant strains: use the malt extract medium of total sugar degree 10-20%, add 2% agar, streak inoculation, under 30-35 ℃ of condition, shaking table was cultivated 20-24 hour, preserved in 4 ℃ of refrigerators;
2) liquid spawn and level liquid are cultivated bacterial classification: use the malt extract medium of total sugar degree 10-20%, the inoculum size of seed liquor is 5-20%, cultivated 20-24 hour at 30-35 ℃ of shaking table, or under 30-35 ℃ of condition, static cultivation 24-48 hour;
3) secondary liquid culture bacterial classification: use sugar degree 10-20% amylum hydrolysate of the sugar liquid, the bacterial classification that the inoculation level liquid is cultivated, inoculum size is 5-20%, cultivated 20-24 hour at 30-35 ℃ of shaking table, or under 30-35 ℃ of condition, static cultivation 24-48 hour;
4) three grades of liquid culture bacterial classifications: use the amylum hydrolysate of the sugar liquid of sugar degree 10-20%, the bacterial classification of inoculation secondary liquid culture, inoculum size is 5-20%, cultivated 20-24 hour at 30-35 ℃ of shaking table, or under 30-35 ℃ of condition, static cultivation 24-48 hour.
5) fermentor tank or vat liquid culture bacterial classification: use amylum hydrolysate of the sugar liquid, sugar degree 10-20%, the bacterial classification of three grades of liquid culture of inoculation, the inoculum size of seed liquor is 5-20%, under 25-35 ℃ condition, and stir culture 20-24 hour, or under 25-35 ℃ condition, static cultivation 24-48.
3, a kind of method of making bioactive micro peptide of plant protein according to claim 1 and 2, it is characterized in that: under the condition that the seed fermentation agent exists, the fermention medium that contains plant protein material is carried out liquid fermenting, and the prescription of fermention medium is (ratio of weight and number):
Plant protein material 30-85
Root of Cornu Cervi Pantotrichum powder 5-10
Wheat bran 5-30
Corn cob 0-30
Semen Maydis powder 0-20
Wheat flour 0-10
Urea or ammonium sulfate 0-0.5
Add water, the gross weight of above-mentioned raw materials: water=1: 1.5-8; The inoculum size of seed fermentation agent is 5-30%; PH6-7; Under 35-50 ℃ the condition, ventilation O
2=25-30%.
4, a kind of method of making bioactive micro peptide of plant protein according to claim 3 is characterized in that: also be added with in inorganic salt, VITAMIN, trace element or the calcium preparation one or more in the described substratum.
5, a kind of method of making bioactive micro peptide of plant protein according to claim 3, it is characterized in that: tunning obtains product after filter press, drying.
6, a kind of method of making bioactive micro peptide of plant protein according to claim 1 and 2, it is characterized in that: under the condition that the seed fermentation agent exists, the fermention medium that contains plant protein material is carried out solid fermentation, and the prescription of fermention medium is (ratio of weight and number):
Plant protein material 30-85
Root of Cornu Cervi Pantotrichum powder 5-10
Wheat bran 5-30
Corn cob 0-30
Semen Maydis powder 0-20
Wheat flour 0-10
Urea or ammonium sulfate 0-0.5
Add water, the gross weight of above-mentioned raw materials: water=1: 0.5-1.5, the inoculum size of seed fermentation agent is 5-30%; Under 15-50 ℃ the condition, static cultivation 1-4 days.
7, a kind of method of making bioactive micro peptide of plant protein according to claim 6 is characterized in that: also be added with in inorganic salt, VITAMIN, trace element or the calcium preparation one or more in the described substratum.
8, a kind of method of making bioactive micro peptide of plant protein according to claim 6 is characterized in that: tunning nature airing or oven drying at low temperature to moisture below 65 ℃ are lower than 12%, pulverize and obtain product.
9, a kind of method of making bioactive micro peptide of plant protein according to claim 1, it is characterized in that: described plant protein material is one or more in leguminous forage powder, garden sorrel, grouts, Zein powder or the bean powder.
10, a kind of method of making bioactive micro peptide of plant protein according to claim 7 is characterized in that: described grouts are one or more in soybean cake dregs, cotton cake dregs, dregs of rapeseed cake, peanut dregs, Sunflower Receptacle grouts, flaxseed grouts or the sesame cake meal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01140321 CN1247792C (en) | 2001-12-07 | 2001-12-07 | Method for preparing bioactive micro peptide of plant protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01140321 CN1247792C (en) | 2001-12-07 | 2001-12-07 | Method for preparing bioactive micro peptide of plant protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1422957A true CN1422957A (en) | 2003-06-11 |
| CN1247792C CN1247792C (en) | 2006-03-29 |
Family
ID=4675798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01140321 Expired - Fee Related CN1247792C (en) | 2001-12-07 | 2001-12-07 | Method for preparing bioactive micro peptide of plant protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1247792C (en) |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399932C (en) * | 2007-08-28 | 2008-07-09 | 张模丹 | Probiotics yeast protein feed |
| CN101433270B (en) * | 2008-12-25 | 2012-04-25 | 南京财经大学 | Preparation method of vegetable seed protein feed |
| CN103283946A (en) * | 2013-06-08 | 2013-09-11 | 浙江大学 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of corncobs and sesame seed cakes |
| CN103609862A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Method for improving feeding nutritive value of sesame seed meal by combining enzymolysis method with fermentation method |
| CN103636928A (en) * | 2013-11-26 | 2014-03-19 | 青岛嘉瑞生物技术有限公司 | Method for preparing functional alga fertilizer through co-fermentation of multiple strains |
| CN103642695A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation |
| CN103734852A (en) * | 2014-01-02 | 2014-04-23 | 江南大学 | Preparation method of aspergillus-oryzae fermented beverage based on wheat-bran bean pulp |
| CN103734851A (en) * | 2014-01-02 | 2014-04-23 | 江南大学 | Method for preparing non-milk probiotic beverage based on aspergillus oryzae culture |
| CN104388513A (en) * | 2014-12-02 | 2015-03-04 | 东莞市荷花食品有限公司 | Method of preparing salty sesame peptide from sesame cake meal by compound enzyme |
| CN104543328A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Preparation method and application for fermented gingko cell protein concentrated solution |
| CN104543832A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Preparation method and application of garlic fermented cell protein concentrated liquor |
| CN104543331A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Method for processing garlic fermentation cell protein and application |
| CN104543332A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Processing method and application for fermented gingko cell proteins |
| CN105124394A (en) * | 2014-06-30 | 2015-12-09 | 罗汉斐 | Corn fermentation nutritious food and producing method thereof |
| CN105285564A (en) * | 2014-06-30 | 2016-02-03 | 雷宗达 | Fermented peanut nutritious food and preparation method thereof |
| CN105624199A (en) * | 2014-11-24 | 2016-06-01 | 施宗雄 | Method for extracting rhizopus intracellular bio-active induced matter and application thereof |
| CN105747082A (en) * | 2016-02-29 | 2016-07-13 | 西华大学 | Fermented compound bran powder and preparation method thereof |
| CN109198189A (en) * | 2018-11-22 | 2019-01-15 | 江门海大饲料有限公司 | A kind of method that bacterium-enzyme cooperative fermentation prepares the corn protein powder of high protein content |
| CN110551790A (en) * | 2019-07-29 | 2019-12-10 | 浙江大学 | Preparation method and application of milk-derived active peptide |
| CN111602813A (en) * | 2020-05-27 | 2020-09-01 | 华熙生物科技股份有限公司 | Preparation method of sunflower disc fermentation stock solution for health food and product thereof |
| CN112586599A (en) * | 2020-12-17 | 2021-04-02 | 江南大学 | Method for preparing peanut meal |
| CN114107414A (en) * | 2021-12-10 | 2022-03-01 | 北海开元生物科技有限公司 | Method for preparing bitter gourd polypeptide by fermentation method |
-
2001
- 2001-12-07 CN CN 01140321 patent/CN1247792C/en not_active Expired - Fee Related
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100399932C (en) * | 2007-08-28 | 2008-07-09 | 张模丹 | Probiotics yeast protein feed |
| CN101433270B (en) * | 2008-12-25 | 2012-04-25 | 南京财经大学 | Preparation method of vegetable seed protein feed |
| CN103283946A (en) * | 2013-06-08 | 2013-09-11 | 浙江大学 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of corncobs and sesame seed cakes |
| CN103283946B (en) * | 2013-06-08 | 2014-07-16 | 浙江大学 | Method for preparing complex enzyme-probiotic preparation for feeds through biotransformation of corncobs and sesame seed cakes |
| CN103642695A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation |
| CN103636928A (en) * | 2013-11-26 | 2014-03-19 | 青岛嘉瑞生物技术有限公司 | Method for preparing functional alga fertilizer through co-fermentation of multiple strains |
| CN103636928B (en) * | 2013-11-26 | 2014-12-10 | 青岛嘉瑞生物技术有限公司 | Method for preparing functional alga fertilizer through co-fermentation of multiple strains |
| CN103609862A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Method for improving feeding nutritive value of sesame seed meal by combining enzymolysis method with fermentation method |
| CN103734852A (en) * | 2014-01-02 | 2014-04-23 | 江南大学 | Preparation method of aspergillus-oryzae fermented beverage based on wheat-bran bean pulp |
| CN103734851A (en) * | 2014-01-02 | 2014-04-23 | 江南大学 | Method for preparing non-milk probiotic beverage based on aspergillus oryzae culture |
| CN103734852B (en) * | 2014-01-02 | 2015-07-08 | 江南大学 | Preparation method of aspergillus-oryzae fermented beverage based on wheat-bran bean pulp |
| CN103734851B (en) * | 2014-01-02 | 2015-06-24 | 江南大学 | Method for preparing non-milk probiotic beverage based on aspergillus oryzae culture |
| CN105285564A (en) * | 2014-06-30 | 2016-02-03 | 雷宗达 | Fermented peanut nutritious food and preparation method thereof |
| CN105124394A (en) * | 2014-06-30 | 2015-12-09 | 罗汉斐 | Corn fermentation nutritious food and producing method thereof |
| CN105624199A (en) * | 2014-11-24 | 2016-06-01 | 施宗雄 | Method for extracting rhizopus intracellular bio-active induced matter and application thereof |
| CN104388513A (en) * | 2014-12-02 | 2015-03-04 | 东莞市荷花食品有限公司 | Method of preparing salty sesame peptide from sesame cake meal by compound enzyme |
| CN104543332A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Processing method and application for fermented gingko cell proteins |
| CN104543331A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Method for processing garlic fermentation cell protein and application |
| CN104543832A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Preparation method and application of garlic fermented cell protein concentrated liquor |
| CN104543328A (en) * | 2014-12-29 | 2015-04-29 | 徐州绿之野生物食品有限公司 | Preparation method and application for fermented gingko cell protein concentrated solution |
| CN105747082A (en) * | 2016-02-29 | 2016-07-13 | 西华大学 | Fermented compound bran powder and preparation method thereof |
| CN109198189A (en) * | 2018-11-22 | 2019-01-15 | 江门海大饲料有限公司 | A kind of method that bacterium-enzyme cooperative fermentation prepares the corn protein powder of high protein content |
| CN110551790A (en) * | 2019-07-29 | 2019-12-10 | 浙江大学 | Preparation method and application of milk-derived active peptide |
| CN111602813A (en) * | 2020-05-27 | 2020-09-01 | 华熙生物科技股份有限公司 | Preparation method of sunflower disc fermentation stock solution for health food and product thereof |
| CN111602813B (en) * | 2020-05-27 | 2022-11-15 | 华熙生物科技股份有限公司 | Preparation method of sunflower disc fermentation stock solution for health food and product thereof |
| CN112586599A (en) * | 2020-12-17 | 2021-04-02 | 江南大学 | Method for preparing peanut meal |
| CN114107414A (en) * | 2021-12-10 | 2022-03-01 | 北海开元生物科技有限公司 | Method for preparing bitter gourd polypeptide by fermentation method |
| CN114107414B (en) * | 2021-12-10 | 2024-03-15 | 北海开元生物科技有限公司 | Method for preparing balsam pear polypeptide by fermentation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1247792C (en) | 2006-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1247792C (en) | Method for preparing bioactive micro peptide of plant protein | |
| CN1199578C (en) | Method for producing straw protein fodder by using microbiological straw fermentation | |
| CN104256057B (en) | A kind of method utilizing alcohol effluent and crop material to prepare forage protein | |
| CN101642187B (en) | Protein feed rich in small peptides and multi-enzyme bacteria and preparation method thereof | |
| CN101182564B (en) | Function aquatic peptide capable of promoting growth of aquatic animals and enhancing disease resistance and method of producing the same | |
| CN110150458A (en) | A method of forage protein is prepared using microbial fermentation black soldier flies larva | |
| CN102669483B (en) | Mixed plant protein fish feed as well as preparation method and application thereof | |
| CN102429163A (en) | Method for preparing tartary buckwheat monascus and application thereof | |
| CN1108053A (en) | Fermented bagasse feed, and its preparation and uses | |
| CN108783106A (en) | A kind of bionical feed of mandarin fish adult fish culture and preparation method thereof | |
| CN1145734A (en) | Quick-fermented feed, its preparation and uses | |
| CN103478417A (en) | Method for producing fermented soybean meal by two-step inoculation segmented solid-state fermentation | |
| CN104247893A (en) | Method for preparing biological fermentation peptide for increasing dairy cattle milk by combination of enzymolysis method and fermentation method | |
| CN103478441B (en) | A kind of rice active bacterium peptide fish meal and application thereof | |
| CN110432400A (en) | A kind of meat goose feed and preparation method thereof improving fattening period feed intake | |
| CN107439844A (en) | A kind of Penaeus Vannmei special swelling feed | |
| CN108432979A (en) | A kind of Australia freshwater lobster ecological feed | |
| CN1139520A (en) | Production method for biological multi-bacterial fermented fodder made from stalks | |
| CN106260589A (en) | A kind of method that recycling wheat bran prepares albumen feedstuff | |
| CN102907568B (en) | Cold-region fermented soybean meal industrialized production method | |
| CN104987153A (en) | Oyster mushroom cultivation material | |
| CN1171539C (en) | Nutrients additive for biological feed of livestock and fowls and its preparing process | |
| CN109170151A (en) | The preparation method of fermentative feedstuff of microbe | |
| CN1537445A (en) | Method for producing forage protein contg. blood powder | |
| CN1248592C (en) | Dehusk and detoxin of cotton-seed cake to produce protein feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| ASS | Succession or assignment of patent right |
Owner name: FEED RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRIC Free format text: FORMER OWNER: RUISI JIAYE FODDER APPLIED TECHNOLOGY RESEARCH CENTER, BEIJING Effective date: 20041022 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20041022 Address after: 100081 feed station, Academy of Agricultural Sciences, 12 South Avenue, Haidian District, Beijing, Zhongguancun Applicant after: Feed Research Institute Chinese Academy of Agricultural Sciences Address before: 100081 feed station, Academy of Agricultural Sciences, 12 South Avenue, Haidian District, Beijing, Zhongguancun Applicant before: Beijing Ruisijiaye Fodder Technology Research Center |
|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Baotou Jiuzhou Dadi Biological Technology Co., Ltd. Assignor: Feed Research Institute Chinese Academy of Agricultural Sciences Contract fulfillment period: 2007.10.18 to 2015.10.17 contract change Contract record no.: 2009990000405 Denomination of invention: Method for preparing bioactive micro peptide of plant protein Granted publication date: 20060329 License type: Exclusive license Record date: 2009.4.30 |
|
| LIC | Patent licence contract for exploitation submitted for record |
Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2007.10.18 TO 2015.10.17; CHANGE OF CONTRACT Name of requester: BAOTOU KYRSHU EARTH BIOISYSTECH CO., LTD. Effective date: 20090430 |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060329 Termination date: 20101207 |