CN1422867A - Method for extracting and separating haematoglobin from animal blood - Google Patents
Method for extracting and separating haematoglobin from animal blood Download PDFInfo
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- CN1422867A CN1422867A CN 01129147 CN01129147A CN1422867A CN 1422867 A CN1422867 A CN 1422867A CN 01129147 CN01129147 CN 01129147 CN 01129147 A CN01129147 A CN 01129147A CN 1422867 A CN1422867 A CN 1422867A
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Abstract
The invention discloses a method to extract and separate hemoglobin from animal blood. The steps: a get blood; after killing animial and bleeding, at once add salt according to 5-20g every litre of blood; b, dissolve by alkali solution; within 48 hours, add 0.5-2 mol alkali and 0.1-1.6 mol urea into every litre of blood and dissolve for 8-48 hours; c, salt out; filter the solution, add acetic acid into the filtrate, make pH value 6-7, and salt out and degrade for 3-6 hours; d, filter, take the deposit anjd wash, e, dry and crush to obtain the hemoglobin.
Description
The present invention relates to a kind of extraction, method of separating protein, relate in particular to a kind of method of from blood, separating, extracting oxyphorase.
Oxyphorase is an erythrocytic main component in high vertebrates and the human blood, have identical chemical structure, material composition and physiological function, the content height of its amino acid and ferrous porphyrin has good benefit iron nutritive value and the unique effects of preventing and treating anaemia with its nutritive food and medicine of making as raw material.The existing method of from animal blood, extracting, separating oxyphorase, requiring earlier blood to be carried out anti-freezing handles, make anticoagulated blood or spraying drying blood meal and could carry out extraction separation again, need to use specific equipment, the processing condition requirement is high, difficulty is big, be applicable to that the large-scale modernized slaughterhouse of R﹠D institution and minority (factory) adopts, most ofs medium and smallly be difficult to accomplish, can't promote, utilize with slaughterhouse (point) rural area.
Purpose of the present invention just provides a kind of blood and does not need that anti-freezing is handled,, the novel method from animal blood extracting, separate oxyphorase that be easy to promote simple without specific equipment, technology.
For achieving the above object, the technical solution used in the present invention is:
A kind of method of from animal blood, extracting, separating oxyphorase, its basic step is:
A, get blood: after slaughtering the animal bloodletting, add the salt of 5～20 grams at once by every liter of blood;
B, alkali are molten: in 48 hours, add 0.5～2 mole alkali, 0.1～1.6 mole urea in every liter of blood, dissolved 8～48 hours;
C, saltout: solution is filtered, adds acetic acid in filtrate, and making the filtrate pH value is 6～7, saltouts, degrades 3～6 hours;
D, filtration, taking precipitate, washing;
E, drying, pulverizing promptly get oxyphorase.
The alkali that adds during above-mentioned b step alkali is molten is at least a in sodium hydroxide, potassium hydroxide, diethylamino, dichloroacetic acid, organic amine, the yellow soda ash.
Above-mentioned a kind of method of from animal blood, extracting, separating oxyphorase, also can in every premium on currency, add 0.5～2 mole alkali, 0.1～1.6 mole urea, add the d step gained throw out of solubilized amount again, dissolve 8～48 hours, carry out the c step then and saltout, d goes on foot filtration; Whole process can be carried out one to three time, carries out e drying, the pulverizing in step subsequently again, makes the oxyphorase elaboration.
Below in conjunction with embodiment the present invention is done to describe in further detail.
Embodiment one: a kind of method of from animal blood, extracting, separating oxyphorase, and its basic step is: a, get blood: after slaughtering the animal bloodletting, (in several minutes) add the salt of 5 grams by every liter of blood at once; B, alkali are molten: in 4 hours, add 0.5 mole sodium hydroxide, 1.6 moles urea in every liter of blood, dissolved 8 hours; C, saltout: solution is filtered, adds acetic acid in filtrate, and making the filtrate pH value is 7, saltouts, degrades 6 hours; D, filtration (be pick up speed, can adopt suction filtration), taking precipitate, washing (purpose of washing is small amounts of water soluble compositions such as removal serum protein); E, drying, pulverizing promptly get oxyphorase.
The oxyphorase that so makes can satisfy the requirement of making nutrition, protective foods fully, but if will be as medicine and other scientific research purposes, then need produce the oxyphorase elaboration, this example can add 0.5 mole sodium hydroxide, 1.6 moles urea in every premium on currency, the d step gained throw out that adds the solubilized amount again, dissolved 8 hours, carry out the c step then and saltout, d goes on foot filtration; Whole process can be carried out one to three time, and (this process multiplicity is many more, and oxyphorase purity is high more, but recovery rate is low more.) carry out e drying, the pulverizing in step subsequently again, make the oxyphorase elaboration.
The d of solubilized amount goes on foot the gained throw out and is meant in the alkali lye of concentration that meets the requirements, in the dissolution time that requires, and the amount that throw out can be dissolved.The alkali lye of the concentration that meets the requirements in this example be 0.5 mole in every premium on currency sodium hydroxide, 1.6 moles urea; General then be the alkali of 0.5～2 mole in every premium on currency, 0.1～1.6 mole urea.The dissolution time that requires, this example is 8 hours, generally can be 8～48 hours when implementing.
Embodiment two: its basic step is: a, get blood: after slaughtering the animal bloodletting, add the salt of 20 grams at once by every liter of blood; B, alkali are molten: in 48 hours, add 2 moles potassium hydroxide, 0.1 mole urea in every liter of blood, dissolved 48 hours; C, saltout: solution is filtered, and adding acetic acid in filtrate, to make the filtrate pH value be 6, saltoutd, degrades 3 hours; D, filtration, taking precipitate, washing; E, drying, pulverizing promptly get oxyphorase.
If need produce the oxyphorase elaboration, can add 2 moles potassium hydroxide, 0.1 mole urea in every premium on currency in this example, add the d step gained throw out of solubilized amount again, dissolve 48 hours, carry out the c step then and saltout, d goes on foot filtration; Whole process can be carried out one to three time, carries out e drying, the pulverizing in step subsequently again, makes the oxyphorase elaboration.
Embodiment three: its basic step is: a, get blood: after slaughtering the animal bloodletting, add the salt of 10 grams at once by every liter of blood; B, alkali are molten: in 12 hours, add 1 mole diethylamino, 0.5 mole urea in every liter of blood, dissolved 12 hours; C, saltout: solution is filtered, and adding acetic acid in filtrate, to make the filtrate pH value be 6.5, saltoutd, degrades 4 hours; D, filtration, taking precipitate, washing; E, drying, pulverizing promptly get oxyphorase.
If need produce the oxyphorase elaboration, then can add 1 mole diethylamino, 0.5 mole urea in every premium on currency in this example, add the d step gained throw out of solubilized amount again, dissolve 12 hours, carry out the c step then and saltout, d goes on foot filtration; Whole process can be carried out one to three time, carry out again subsequently e step drying, pulverize and make the oxyphorase elaboration.
The present invention in force, the alkali that adds during b step alkali is molten is except that the sodium hydroxide that can be embodiment one, two, three, potassium hydroxide, diethylamino, also at least a in sodium hydroxide, potassium hydroxide, diethylamino, dichloroacetic acid, organic amine, the yellow soda ash, as long as its alkali concn total amount meets the demands.
Method of the present invention can be widely used in from various animal blood such as pig, ox, sheep and separate, extracts oxyphorase.
Among the present invention, oxyphorase extracts with the isolating principles of chemistry and is: add salt and prevent that big grumeleuse from appearring in blood, make solubilizing agent with urea subsequently, the caustic solubility oxyphorase in the blood is dissolved in alkali lye; Utilize acetic acid to form the buffer solution system of pH value 6-7 again, oxyphorase is salted out under its iso-electric point (PH6-7), thus separated extraction.
Compared with prior art, the present invention has following obvious advantage:
One, blood need not carry out the anti-freezing processing, also need not special equipment, adopts common filtration etc. Equipment gets final product. Technology is simple, and specification requirement is low, less investment, and production cost is low, is suitable for China Promote the use of in vast middle-size and small-size slaughterhouse and slaughterhouse, rural area (point), be conducive to China amount big and Fully effectively utilizing of the animal blood resource of disperseing has good social benefit.
Two, advanced technology, rationally, is 2-5% at hemoglobin recovery rate height, reaches as high as 10%; The extract purity height, its content of hemoglobin is greater than 95%; Extract can be used for making Various have the iron of benefit and prevent and treat nutraceutical and the medicine of anaemia effect, of many uses, before the market Scape is wide, tempting.
Claims (3)
1, a kind of method of from animal blood, extracting, separating oxyphorase, its basic step is:
A, get blood: after slaughtering the animal bloodletting, add the salt of 5～20 grams at once by every liter of blood;
B, alkali are molten: in 48 hours, add 0.5～2 mole alkali, 0.1～1.6 mole urea in every liter of blood, dissolved 8～48 hours;
C, saltout: solution is filtered, adds acetic acid in filtrate, and making the filtrate pH value is 6～7, saltouts, degrades 3～6 hours;
D, filtration, taking precipitate, washing;
E, drying, pulverizing promptly get oxyphorase.
2, a kind of method of extracting from animal blood, separating oxyphorase according to claim 1 is characterized in that: the alkali that adds during described b step alkali is molten is at least a in sodium hydroxide, potassium hydroxide, diethylamino, dichloroacetic acid, organic amine, the yellow soda ash.
3, a kind of method of from animal blood, extracting, separating oxyphorase according to claim 1, it is characterized in that: in every premium on currency, add 0.5～2 mole alkali, 0.1～1.6 mole urea, the d step gained throw out that adds the solubilized amount again, dissolved 8～48 hours, carry out the c step then and saltout, d goes on foot filtration; Whole process can be carried out one to three time, carries out e drying, the pulverizing in step subsequently again, makes the oxyphorase elaboration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01129147 CN1422867A (en) | 2001-12-05 | 2001-12-05 | Method for extracting and separating haematoglobin from animal blood |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01129147 CN1422867A (en) | 2001-12-05 | 2001-12-05 | Method for extracting and separating haematoglobin from animal blood |
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| CN1422867A true CN1422867A (en) | 2003-06-11 |
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| CN 01129147 Pending CN1422867A (en) | 2001-12-05 | 2001-12-05 | Method for extracting and separating haematoglobin from animal blood |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101941975A (en) * | 2010-08-19 | 2011-01-12 | 四川省德阳市生化制品有限公司 | Method for preparing heme from animal blood corpuscle powder serving as raw material |
| CN116686933A (en) * | 2023-06-14 | 2023-09-05 | 黄安根 | Preparation method of pure bovine hemoglobin pigment |
-
2001
- 2001-12-05 CN CN 01129147 patent/CN1422867A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101941975A (en) * | 2010-08-19 | 2011-01-12 | 四川省德阳市生化制品有限公司 | Method for preparing heme from animal blood corpuscle powder serving as raw material |
| CN101941975B (en) * | 2010-08-19 | 2014-01-15 | 四川德博尔制药有限公司 | Method for preparing heme from animal blood corpuscle powder serving as raw material |
| CN116686933A (en) * | 2023-06-14 | 2023-09-05 | 黄安根 | Preparation method of pure bovine hemoglobin pigment |
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