CN1412302A - Method for preparing transfer mitochondrial genome mouse - Google Patents
Method for preparing transfer mitochondrial genome mouse Download PDFInfo
- Publication number
- CN1412302A CN1412302A CN 01136455 CN01136455A CN1412302A CN 1412302 A CN1412302 A CN 1412302A CN 01136455 CN01136455 CN 01136455 CN 01136455 A CN01136455 A CN 01136455A CN 1412302 A CN1412302 A CN 1412302A
- Authority
- CN
- China
- Prior art keywords
- mouse
- cells
- cell
- plastosome
- cytosome
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The method for preparing transfer nitochondrial genoma mouse includes the following steps: introducing heterogenic mitochondrial genome into mouse embryonic stem cell with caryogenetic marker, then according to the classical method for preparing transgenic mouse introducing the embryonic stem cell into early blastosphere of mouse line with different caryogenetic markers to form mosaic mouse, then making the female progeny mouse with mosaicism and mouse of general line implement hybridization, and making identification and screening of nitrochondrial genomes of female mouses in obtained posterities, only using the mouse with heterogenic mitochondrial genoma as seed.
Description
What the present invention relates to is a kind of method for preparing transfer mitochondrial genome mouse.
Biology general knowledge is told us, and all eukaryotes all have the mechanism of two cover heredity at least: nuclear gene group and Mitochondrial Genome Overview.The gene that the former carries is by the heredity of Mendelian's mode, and the latter promptly passes to the offspring by ovum then by matrilinear inheritance.The goal gene that method shifted of existing preparation transgenic mice all is a nuclear gene.
The objective of the invention is to propose a kind of method for preparing transfer mitochondrial genome mouse, it also can be used for preparing and have genomic other animals of transfer mitochondrial in principle.The animal of this strain is all holding out broad prospects aspect theory research and the practical application, as be used for: influence, the cultivation that the inherited disease of research human mitochondrial genetic flaw, research plastosome are grown animal embryo has the nuclear-matter hybrid livestock of high productivity energy and clones rare and endangered animal, or the like.
Method steps of the present invention is as follows:
One, the plastosome that will have the mouse embryo stem cell (ES cell) of nuclear genetic mark (as the mark of control color inheritance) is removed, and replaces allogenic plastosome.Concrete operations are as follows:
1. mouse ES cells and heterozoic cell are cultivated respectively on suitable substrate.
2. two kinds of cells will cultivating are handled with cytochalasin respectively.
3. two kinds of cells will handling are centrifugal respectively, obtain corresponding nucleome and cytosome.
4. sampling detects the treatment effect of above-mentioned nucleome and cytosome, adjusts treatment process, makes in the nucleome of (1) mouse ES cells not contain or contain hardly plastosome; (2) not residual in the heterocellular cytosome have any nucleus.
5. will reach above-mentioned (1) uses the method for PEG (polyoxyethylene glycol) or electric shock to merge with two kinds of compositions that (2) require.
6. above-mentioned fused cell is cultivated for some time under proper condition.
Two, will be in the early stage blastaea of the mouse species that has different nuclear genetic marks through the ES injection cell of above-mentioned processing.
Three, above-mentioned blastaea is transplanted in the uterus of the female mouse of replace-conceive, is raised until giving birth to sub-mouse.As long as graft procedure is abundant, that always can find to have the nuclear genetic mark in this a little mouse inlays proterties (as variegated fur) person.
Four, get the above-mentioned female mice and the generally mouse hybridization of strain of inlaying proterties that have.Female mice in generation in institute postpartum is carried out the evaluation and screening of Mitochondrial Genome Overview, only have kind of the usefulness that gives over to of external source Mitochondrial Genome Overview.
Five, with the male mice mating breeding of above-mentioned mouse with general strain, the quantity of amplification female mice as kind of a usefulness, is set up the mouse species that carries the external source Mitochondrial Genome Overview.
Effect of the present invention is can obtain the genomic mouse of transfer mitochondrial, and this proterties can stably pass to the offspring by maternal side.
Claims (2)
1. a method for preparing transgenic animal is characterized in that, what shifted is whole Mitochondrial Genome Overview, and its operation steps comprises: the plastosome that will have the mouse embryo stem cell (ES cell) of nuclear genetic mark is removed, and replaces allogenic plastosome.
2. the method for claim 1 is characterized in that, described removal line grain method comprises the steps:
(1) mouse ES cells and heterozoic cell are cultivated respectively on suitable substrate;
(2) two kinds of cells will cultivating are handled with cytochalasin respectively;
(3) two kinds of cells will handling are centrifugal respectively, obtain corresponding nucleome and cytosome;
(4) sampling detects the treatment effect of above-mentioned nucleome and cytosome, adjusts treatment process,
Make not contain or contain hardly in the nucleome of mouse ES cells and not residual in plastosome, the heterocellular cytosome any nucleus arranged;
(5) will reach of the method fusion of two kinds of compositions of above-mentioned requirements with PEG (polyoxyethylene glycol) or electric shock;
(6) above-mentioned fused cell is cultivated for some time under proper condition, be then injected in the blastaea, to obtain inlaying mouse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01136455 CN1247773C (en) | 2001-10-18 | 2001-10-18 | Method for preparing transfer mitochondrial genome mouse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01136455 CN1247773C (en) | 2001-10-18 | 2001-10-18 | Method for preparing transfer mitochondrial genome mouse |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1412302A true CN1412302A (en) | 2003-04-23 |
| CN1247773C CN1247773C (en) | 2006-03-29 |
Family
ID=4673672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01136455 Expired - Fee Related CN1247773C (en) | 2001-10-18 | 2001-10-18 | Method for preparing transfer mitochondrial genome mouse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1247773C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502248B (en) * | 2008-11-05 | 2011-07-27 | 东华大学 | Construction of experimental mouse line C57BL/6J-MitC3H/He with substituted mitochondria |
| CN110777167A (en) * | 2019-11-06 | 2020-02-11 | 广州市妇女儿童医疗中心 | Method for constructing mouse model with movement dysfunction phenotype GTPCH enzyme deficiency disease |
-
2001
- 2001-10-18 CN CN 01136455 patent/CN1247773C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502248B (en) * | 2008-11-05 | 2011-07-27 | 东华大学 | Construction of experimental mouse line C57BL/6J-MitC3H/He with substituted mitochondria |
| CN110777167A (en) * | 2019-11-06 | 2020-02-11 | 广州市妇女儿童医疗中心 | Method for constructing mouse model with movement dysfunction phenotype GTPCH enzyme deficiency disease |
| CN110777167B (en) * | 2019-11-06 | 2021-07-06 | 广州市妇女儿童医疗中心 | Method for constructing mouse model with movement dysfunction phenotype GTPCH enzyme deficiency disease |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1247773C (en) | 2006-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2016119703A1 (en) | Method for conducting site-specific modification on entire plant via gene transient expression | |
| CN101195833B (en) | Low-temperature incubation transgene method for cultivated silkworm diapause breed variety | |
| CN107022562A (en) | Utilize method of the CRISPR/Cas9 systems to corn gene rite-directed mutagenesis | |
| CN111763687B (en) | Method for rapidly cultivating corn haploid induction line based on gene editing technology | |
| CN112899247B (en) | Male sterile gene ZmTKPR1 and application thereof in creating male sterile line of corn | |
| CN107022561A (en) | Culture medium and cultural method for cultivating transgenic corns | |
| CN110129455A (en) | A kind of application growing relevant molecular labeling in litopenaeus vannamei genetic breeding | |
| CN106818552A (en) | A kind of method that Heterologous Sperm induces Mandarin fish artificial gynogenesis | |
| CN103725676B (en) | Target sequence suitable for transgene fixed point insertion of silkworm W-chromosome and locus and application thereof | |
| CN114657264A (en) | Clarias fuscus gender-specific molecular marker primer and application thereof | |
| Zhang et al. | Embryogenic callus induction from immature zygotic embryos and genetic transformation of Larix kaempferi 3x Larix gmelinii 9 | |
| CN112680461B (en) | Male sterile gene ZmPHD11 and application thereof in creating male sterile line of corn | |
| CN107805632A (en) | OsMKK6 albumen and encoding gene are in the regulation and control developmental application of vegetable seeds | |
| CN116769796B (en) | ZmENR1 and application of coded protein thereof in corn fertility control | |
| CN113005128A (en) | Male sterile gene ZmMYB84 and application thereof in creating male sterile line of corn | |
| CN1247773C (en) | Method for preparing transfer mitochondrial genome mouse | |
| CN110205333B (en) | Corn dwarf disease induction gene P3a and construction method and application of genetic transformation system thereof | |
| CN107236810A (en) | Corn male nuclear sterile gene, its molecular labeling and application | |
| CN102559691B (en) | Silkworm sex chromosome linked gene PdpI and recessive lethal mutation gene thereof and application of silkworm sex chromosome linked gene PdpI and recessive lethal mutation gene in silkworm sex ratio control | |
| CN102676576B (en) | Method for improving moisture resistance of cabbage type rape | |
| Moffat | Moving forest trees into the modern genetics era | |
| CN106359073A (en) | Androgenesis inductive gene expression cassette, androgenesis inductive expression carrier, and application thereof | |
| CN105494082B (en) | A kind of form based on maize immature embryos differentiates haploid method | |
| CN110592122A (en) | Zebra fish naalad2 gene promoter and application thereof | |
| CN103497970B (en) | Gene transfer method with corn ovule as receptor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |